Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols

Two ‘turn on’ TCF-based fluorescence probes were developed for the detection of biological thiols (TCF-GSH and TCFCl-GSH). TCF-GSH was shown to have a high sensitivity towards glutathione (GSH) with a 0.28 μM limit of detection. Unfortunately, at higher GSH concentrations the fluorescence intensity of TCF-GSH decreased and toxicity was observed for TCF-GSH in live cells. However, TCFCl-GSH was shown to be able to detect GSH at biologically relevant concentrations with a 0.45 μM limit of detection. No toxicity was found for TCFCl-GSH and a clear ‘turn on’ with good photostability was observed for the exogenous addition of GSH, Cys and HCys. Furthermore, TCFCl-GSH was used to evaluate the effects of drug treatment on the levels of GSH in live cells

STECF Fisheries Dependent Information – FDI (STECF-19-11)

Commission Decision of 25 February 2016 setting up a Scientific, Technical and Economic Committee for Fisheries, C(2016) 1084, OJ C 74, 26.2.2016, p. 4–10. The Commission may consult the group on any matter relating to marine and fisheries biology, fishing gear technology, fisheries economics, fisheries governance, ecosystem effects of fisheries, aquaculture or similar disciplines. The STECF reviewed the report of the EWG on Fisheries-dependent Information during its winter 2019 plenary meeting

Report on the STECF Expert Working Group 17-12 Fisheries Dependent Information: ‘New-FDI’

The STECF expert working group (EWG) on Fisheries Dependant Information (FDI) took place in JRC, Ispra from 23 to 27 October 2017 to review the data transmitted by Member States under a new data call (‘New-FDI’). The new data call specification was designed with three broad aims in mind i) Compatibility between the New-FDI data and the data held in the Fleet Economic database. ii) Ability to encompass all EU registered vessels including those from the Mediterranean, Black Sea and external waters fleets. iii) Ability to assess effects of management measures. The main purpose of the EWG was to judge if the call specification was appropriate to accomplish the above aims and to consider any difficulties encountered by member states in fulfilling the data call. Two terms of reference also allowed trial analyses to be conducted of a type relevant to the third broad aim. The EWG addressed all Terms of Reference during the meeting and drew conclusions on the modifications required for the New-FDI data call going forwards. Prior to the EWG it had been agreed by STECF Bureau that the report of the meeting would not be presented to STECF for approval as an STECF report but published separately (as a JRC technical report). This report therefore presents the data, methods observations and findings of an EWG of the STECF but the findings presented in this report do not necessarily constitute the opinion of the STECF or reflect the views of the European Commission and in no way anticipate the Commission’s future policy in this area.JRC.D.2-Water and Marine Resource

Regional variability in peatland burning at mid- to high-latitudes during the Holocene

Acknowledgements This work developed from the PAGES (Past Global Changes) C-PEAT (Carbon in Peat on EArth through Time) working group. PAGES has been supported by the US National Science Foundation, Swiss National Science Foundation, Swiss Academy of Sciences and Chinese Academy of Sciences. We acknowledge the following financial support: UK Natural Environment Research Council Training Grants NE/L002574/1 (T.G.S.) and NE/S007458/1 (R.E.F.); Dutch Foundation for the Conservation of Irish Bogs, Quaternary Research Association and Leverhulme Trust RPG-2021-354 (G.T.S); the Academy of Finland (M.V); PAI/SIA 80002 and FONDECYT Iniciación 11220705 - ANID, Chile (C.A.M.); R20F0002 (PATSER) ANID Chile (R.D.M.); Swedish Strategic Research Area (SRA) MERGE (ModElling the Regional and Global Earth system) (M.J.G.); Polish National Science Centre Grant number NCN 2018/29/B/ST10/00120 (K.A.); Russian Science Foundation Grant No. 19-14-00102 (Y.A.M.); University of Latvia Grant No. AAp2016/B041/Zd2016/AZ03 and the Estonian Science Council grant PRG323 (TrackLag) (N.S. and A.M.); U.S. Geological Survey Land Change Science/Climate Research & Development Program (M.J., L.A., and D.W.); German Research Foundation (DFG), grant MA 8083/2-1 (P.M.) and grant BL 563/19-1 (K.H.K.); German Academic Exchange Service (DAAD), grant no. 57044554, Faculty of Geosciences, University of Münster, and Bavarian University Centre for Latin America (BAYLAT) (K.H.K). Records from the Global Charcoal Database supplemented this work and therefore we would like to thank the contributors and managers of this open-source resource. We also thank Annica Greisman, Jennifer Shiller, Fredrik Olsson and Simon van Bellen for contributing charcoal data to our analyses. Any use of trade, firm, or product name is for descriptive purposes only and does not imply endorsement by the U.S. Government.Peer reviewedPostprin

Regional variability in peatland burning at mid-to high-latitudes during the Holocene

Northern peatlands store globally-important amounts of carbon in the form of partly decomposed plant detritus. Drying associated with climate and land-use change may lead to increased fire frequency and severity in peatlands and the rapid loss of carbon to the atmosphere. However, our understanding of the patterns and drivers of peatland burning on an appropriate decadal to millennial timescale relies heavily on individual site-based reconstructions. For the first time, we synthesise peatland macrocharcoal records from across North America, Europe, and Patagonia to reveal regional variation in peatland burning during the Holocene. We used an existing database of proximal sedimentary charcoal to represent regional burning trends in the wider landscape for each region. Long-term trends in peatland burning appear to be largely climate driven, with human activities likely having an increasing influence in the late Holocene. Warmer conditions during the Holocene Thermal Maximum (∼9–6 cal. ka BP) were associated with greater peatland burning in North America's Atlantic coast, southern Scandinavia and the Baltics, and Patagonia. Since the Little Ice Age, peatland burning has declined across North America and in some areas of Europe. This decline is mirrored by a decrease in wider landscape burning in some, but not all sub-regions, linked to fire-suppression policies, and landscape fragmentation caused by agricultural expansion. Peatlands demonstrate lower susceptibility to burning than the wider landscape in several instances, probably because of autogenic processes that maintain high levels of near-surface wetness even during drought. Nonetheless, widespread drying and degradation of peatlands, particularly in Europe, has likely increased their vulnerability to burning in recent centuries. Consequently, peatland restoration efforts are important to mitigate the risk of peatland fire under a changing climate. Finally, we make recommendations for future research to improve our understanding of the controls on peatland fires

Time Dependent Deterioration of the X-Ray Dental Diagnostic Equipment

Modern dental X-ray examinations are essential for diagnosis. The goal of this paper is to demonstrate time dependent behavior of dose providing parameters, which help to determine equipment’s age effects on the x-ray machine parameters. Also the comparisons between two different dental X-ray generator equipment types half-period and high frequency was made

The Solution of the Heat Conduction Equation in 3D Anisotropic Environment and Possibilities of its Improvement

Nowadays there are high speed improvements in processors frequencies and processors amount on a single map. So these opportunities have to be used in such fields as modeling and simulation, prediction models and simulations. One of these fields is strictly connected with the article's subject (heat conduction). Heat conduction calculation in 3D space is quite a problem for 3D space calculations because the time spent on calculation for usual approaches is quite long. It is possible to separate full iteration of heat conduction calculation into several portions. These portions of calculation could contain separate calculation blocks. It is possible to implement using ADI (Alternating Direction Implicit) principles in dividing full iteration of heat conduction calculation into 3 parts. Each of these parts ignores one of the directions of coordinate axes, but allows to calculate only three diagonal matrix using Thomas algorithm. It means that additional effort on difference scheme construction has the payback of calculation time reducing because of separated calculable blocks. Another boost of calculation speed is dynamic time step implementation by taking into account the prediction matrix of next iteration heat transfer calculations. This approach has no strict impact on time step calculations for each next iteration, but it can be bordered between the possible minimal and maximal time step defined values. Provided solutions allow to manage algorithm calculation time by applying as many computers as many times as needed to reduce the calculation time