68 research outputs found
Symmetrical Skin Lesions on the Gluteal Region in a Patient with Anti-Laminin-332 Mucous Membrane Pemphigoid
Mucous membrane pemphigoid (MMP), previously
called cicatricial pemphigoid, is a rare subepidermal
immunobullous disorder that primarily affects
the mucous membranes (1,2). MMP is divided
into two major subtypes, anti-BP180-type MMP and
anti-laminin-332 (previously called laminin 5 or epiligrin)
MMP. Anti-laminin-332 MMP is known to be
associated with malignant tumors (3), which may
cause overexpression of autoantibodies and induce
autoimmunity to laminin-332 (4). MMP primarily affects
the mucous membranes, and widespread skin
lesions are rare. In MMP, circumscribed skin lesions
have been previously reported as occurring on the
head, neck, and upper trunk (5). We report a case of
anti-laminin-332 MMP presenting with symmetrical
skin lesions characteristic of MMP on the weightbearing
areas of the gluteal regio
Symmetrical Skin Lesions on the Gluteal Region in a Patient with Anti-Laminin-332 Mucous Membrane Pemphigoid
Mucous membrane pemphigoid (MMP), previously
called cicatricial pemphigoid, is a rare subepidermal
immunobullous disorder that primarily affects
the mucous membranes (1,2). MMP is divided
into two major subtypes, anti-BP180-type MMP and
anti-laminin-332 (previously called laminin 5 or epiligrin)
MMP. Anti-laminin-332 MMP is known to be
associated with malignant tumors (3), which may
cause overexpression of autoantibodies and induce
autoimmunity to laminin-332 (4). MMP primarily affects
the mucous membranes, and widespread skin
lesions are rare. In MMP, circumscribed skin lesions
have been previously reported as occurring on the
head, neck, and upper trunk (5). We report a case of
anti-laminin-332 MMP presenting with symmetrical
skin lesions characteristic of MMP on the weightbearing
areas of the gluteal regio
Editorial: Conflicts
The Editorial Board reflects on the theme of 'conflict', as observed in the work published in this issue, and in the wider world
Role of PSCs in Regeneration of Remnant Pancreas after PX
Background and objectives
Mechanism of regeneration of remnant pancreas after partial pancreatectomy (PX) is still unknown. In this study, effect of siRNA against the collagen specific chaperone, HSP47, which inhibits collagen secretion from activated pancreas stellate cells (aPSCs), and induces their apoptosis, on regeneration of remnant pancreas was determined.
Methods
Pancreatectomy was performed according to established methods. Proliferation of cells was assessed by BrdU incorporation. Immunostaining of HSP47 was employed to identify PSCs. Progenitor cells were identified by SOX9 staining. Acinar cells were immunostained for amylase. Co-culture of acinar cells with aPSCs were carried out in a double chamber with a cell culture insert. siRNA HSP47 encapsulated in vitamin A-coupled liposome (VA-lip siRNA HSP47) was delivered to aPSCs by iv injection.
Results
In remnant pancreas of 90% PX rat, new areas of foci were located separately from duodenal areas with normal pancreatic features. After PX, BrdU uptake of acinar cells and islet cells significantly increased, but was suppressed by treatment with VA-lip siRNA HSP47. BrdU uptake by acinar cells was augmented by co-culturing with aPSCs and the augmentation was nullified by siRNA HSP47. BrdU uptake by progenitor cells in foci area was slightly enhanced by the same treatment. New area which exhibited intermediate features between those of duodenal and area of foci, emerged after the treatment.
Conclusion
aPSCs play a crucial role in regeneration of remnant pancreas, proliferation of acinar and islet cells after PX through the activity of secreted collagen. Characterization of new area emerged by siRNA HSP47 treatment as to its origin is a future task
- …