The technology of programmed learning for additional professional education

The article deals with the technology of programmed learning for additional professional education. Marked peculiarities of the technology of programmed learningВ статье рассмотрена технология программированного обучения для дополнительного профессионального образования. Обозначены особенности технологии программированного обучени

Competence-based approach to training and retraining

The article deals with the problems of training and retraining of personnel in programs of additional education based on the competence approach. The features of the competence approach are indicated. Special attention is paid to the implementation of the competence approach. The unsolved problems of the competence approach are consideredВ статье рассмотрена проблема подготовки и переподготовки персонала по программам дополнительного образования, основанным на компетентностном подходе. Обозначены особенности компетентностного подхода. Особое внимание уделено реализациям компетентностного подхода. Рассматриваются не решенные проблемы компетентностного подход

The usage of a binder system for frozen berries in the manufacture of confectionery

The aim of the research was to create binding systems for confectionery using gelling agents. The possibility of using partially hydrolyzed liquid egg white (egg hydrolyzate) in the binding system of gelling agents (egg hydrolyzate - agar (EG-A), egg hydrolyzate - starch (EG-S)) was determined to obtain the required mechanical characteristics when creating coatings, ornaments or fillers in confectionery with whole berries or pieces of fruit. In this regard, a technology has been developed for the hydrolysis of liquid egg white in the presence of an acidic reagent. The best rheological characteristics of the gelling agent from egg white were obtained under the following hydrolysis conditions: egg white : 1% HCl ratio = 1:2, process temperature - 66°C, duration - 40 minutes, the pH of the egg hydrolyzate was 6.53, the amount of dry solids was 11.78%. The newly created systems with agar (E406, Germany), chemically modified food starch (E1442, Germany) and hydrolyzed egg white (egg hydrolyzate) allow to adjust the properties of the coating for quick-frozen berries used in semi-finished confectionaries or cakes. It is established that the coating for quick-frozen berries, which includes a system consisting of 1% E406 and 0.5% egg hydrolyzate, should be carried out in 2 stages. In the first stage, a 10-minute exposure of the coating, which has a tensile strength of 580 g cm-2 , allows to create a strong capsule around the berry, which prevents the processes of destruction from proceeding. The second stage is necessary to obtain a uniform surface coating of the semi-finished mix from the berries. In this case, the tensile strength of the coating should be 480 g cm-2 . The system created from the E1442 and egg hydrolyzate gelling agents was also used in 2 stages when making cakes, which were subsequently baked at 180 °C. The content of the binding system in the coating applied to the test substrate was 6.9–7.7%, the effective viscosity of the coating was 120–180 Pa s. The content of the binding system in the coating of the surface of the berries in the second stage was 5.2–6.3% with effective viscosity values of 50–90 Pa s. Semi-finished berry products and ready-made baked cakes, produced with developed binding systems, can be stored at -8 °C for 10 to 12 days. After refrigerated storage, the separated moisture was not observed in the test samples. The absence of the phenomenon of syneresis with the indicated proportions of the introduction of gelling agents in coating systems has been established

CLINICO-BIOENERGETIC RATIONALE FOR THE USE OF PHYTOTHERAPY IN PATIENTS WITH TRAUMATIC BRAIN DISEASE AND ARTHRITIS ACCOMPANIED BY AUTONOMIC DISORDERS

The subject of the study is the establishment of the value of the effectiveness of the application of a complex of phytosbores obtained with the help of bioenergetic technologies in patients with vegetative disorders that aggravate the course of the underlying disease during the planned course of rehabilitation. For this purpose, 97 patients with traumatic brain disease and rheumatoid arthritis with vegetative disorders were examined. All examined were divided into 2 groups: the main group consisted of 64 patients, in the complex rehabilitation of which, along with medicinal preparations (dehydration, resorptive, vasoactive, nootropic, sedative, vitamin), the intake of six phytobes obtained by bioenergetic technologies was used. Complex therapy from phytospores was taken internally. Patients of the main group after the completion of the course of treatment noted a significant subjective improvement, correlating with the results of clinical objective examination and with instrumental data, autonomic samples (when they are repeated at the end of the course of treatment). The advantage of this method is the absence of side effects and complications, the possibility of using both inpatient and outpatient settings, both during the planned course of treatment, and to achieve a supportive effect during periods of remission.Key words: phytotherapy, phytospora complex, closed craniocerebral trauma, traumatic brain disease, autonomic disorders, rheumatoid arthritis.*Доктор медицины и философии, профессор Павлусенко И. И., *Старший научный сотрудник, доцент Макеева М. А., **Главный врач, Ярошенко И. А., ***Павлусенко В. И. Клинико-биоэнергетическое обоснование применения фитотерапии у больных с травматической болезнью головного мозга и артритом, сопровождающимися вегетативными расстройствами/ *Eвропейский университет функциональной медицины, Украина, Киев; **Харьковская клиническая больница на железнодорожном транспорте № 2, Украина, Xарьков; ***Национальный медицинский университет им. Богомольца, Украина, КиевПредметом исследования является установление значения эффективности применения комплекса фитосборов, полученных с помощью биоэнергетическиx теxнологий, у пациентов с вегетативными нарушениями, отягощающиx течение основного заболевания в процессе планового курса реабилитации. С этой целью обследовано 97 больных с травматической болезнью головного мозга и ревматоидным артритом, страдающих вегетативными расстройствами. Все обследованные были разделены на 2 группы: основную группу составили 64 больных, в комплексной реабилитации которых, наряду с медикаментозными препаратами (дегидратационными, рассасывающими, вазоактивными, ноотропными, седативными, витаминными), использовался приём 6 фитосборов, полученных с помощью биоэнергетическиx теxнологий. Комплексная терапия из фитосборов принималась внутрь. Больные основной группы после завершения курса лечения отмечали значительное субъективное улучшение, коррелирующее с результатами клинического объективного осмотра и с инструментальными данными, вегетативными пробами (при их повторном проведении в конце курса лечения). Достоинством данного метода является отсутствие побочных действий и осложнений, возможность применения как в стационаре, так и в амбулаторных условиях, как в процессе планового курса лечения, так и для достижения поддерживающего эффекта в периоды ремиссий. Ключевые слова: фитотерапия, комплекс фитосборов, закрытая черепно-мозговая травма, травматическая болезнь головного мозга, вегетативные нарушения, ревматоидный артрит

Successful long-term prophylaxis of hereditary pregnancy-associated angioedema with plasma-derived C1-inhibitor concentrate: a case report

Hereditary angioedema (HAE) is a rare autosomal dominant disease caused by quantitative (type I) or functional (type II) deficiency in C1 esterase inhibitor (C1-INH). It may be caused by new mutations in up to 20% of patients. Prevalence of HAE is uncertain but is estimated to be approximately 1 case per 50,000 persons, without known differences among ethnic groups. C1-INH protein is a serine protease inhibitor that is important in controlling vascular permeability by acting on the initial phase of the complement activation, blood clotting, and fibrinolysis. Deficiency in functional C1-INH protein permits release of bradykinin, a key mediator of vascular permeability. Symptoms typically begin since childhood, worsening at puberty, and persist throughout the life, with unpredictable clinical course. The patients with HAE suffer from recurrent, acute attacks of edema that can affect any body sites, causing potentially life-threatening disorders (laryngeal edema). Results of clinical studies show that minor traumas, stress and medical interventions may be frequent precipitants of swelling episodes, but many attacks occur without an apparent cause. Pregnancy-associated hormonal changes may affect the course of C1-INH angioedema attacks by worsening, improving, or having no impact at all, but a higher percentage of pregnant women experienced an increase in C1-INH-HAE attack rates. Therapeutic options for patients with HAE are limited during pregnancy. C1-INH concentrate is recommended as the first-line therapy for pregnant women with HAE for on-demand treatment, shortterm and long-term prophylaxis, due to its safety and efficiency. Other therapies, e.g., treatment with fresh frozen plasma, androgens, icatibant, antifibrinolytics, may show variable efficacy, or cause undesirable side effects. The case below illustrates the successful treatment of HAE in a pregnant woman with C1 esterase inhibitor (C1-INH) concentrate. This patient had a very mild course of HAE during her lifetime and didn’t get any treatment. During pregnancy, she experienced a significant increase in the frequency of attacks, and the decision was made to start replacement therapy with a plasma-derived, double virus-inactivated C1-INH concentrate as a long-term prophylaxis throughout the full term of her pregnancy, before, during and after the cesarean section delivery

ASSESSMENT OF RADIATION RISKS WITH TIME-DISTRIBUTED EXPOSURE

This article assesses the radiation risks of occupational exposure. Based on the calculations obtained, conclusions are drawn about the discrepancy between radiation safety standards in accordance with dose limits and radiation risks

PHYTOSORTS «FAMILY HEALTH» IN OPTIMIZING THE REHABILITATION OF PATIENTS WITH VERTEBROGENIC PAIN SYNDROMES

The subject of the study is the establishment of the value of the effectiveness of the application of a complex of phytosbores obtained with the help of bioenergetic technologies in patients with vertebral pathology of the spine. The use of therapy using the standard treatment regimen and the integrated use of 5 phytosolves "Family Health" obtained with the help of bioenergetic technologies is substantiated. For this purpose, 45 patients with pain syndrome of cervicothoracic and lumbosacral localization were examined, who, in the course of the planned course of in-patient rehabilitation, along with the standard treatment scheme, received a phytocomplex from 5 phytospores. The control group was also made up of 45 patients with the presence of a similar pain syndrome without using a complex of phytospores in the rehabilitation process. Complex therapy from phytospores was taken internally. Patients of the main group after completion of the course of treatment noted significant subjective improvement, correlating with the results of clinical objective examination and with the data of scales. The advantage of this method is the absence of side effects and complications, the possibility of using both inpatient and outpatient settings, both during the planned course of treatment, and to achieve a supportive effect during periods of remission.Key words: phytotherapy, phytospora complex, phytotherapy, phytospora complex, cervicalgia, osteochondrosis, lower back pain.*Доктор медицины и философии, профессор Павлусенко И. И., *старший научный сотрудник, доцент Макеева М. А., **врач-ревматолог высшей категории, Жерновенков А. А., ***Павлусенко В. И. Фитосборы «Здоровье семьи» в оптимизации реабилитации больных с вертеброгенными болевыми синдромами/ *Eвропейский университет функциональной медицины, Украина, Киев; **Харьковская клиническая больница на железнодорожном транспорте № 2, Украина, Xарьков; ***Национальный медицинский университет им. Богомольца, Украина, КиевПредметом исследования является установление значения эффективности применения комплекса фитосборов, полученных с помощью биоэнергетическиx теxнологий, у пациентов с вертеброгенной патологией позвоночника. Обосновано применение терапии с использованием стандартной схемы лечения и комплексного использования 5 фитосборов «Здоровье семьи» полученных с помощью биоэнергетическиx теxнологий. С этой целью обследовано 45 больных с болевым синдромом шейно-грудной и пояснично-крестцовой локализации, которые в процессе планового курса стационарной реабилитации, наряду с использованием стандартной сxeмы лечения получали фитокомплекс из 5 фитосборов. Контрольную группу составили также 45 пациентов с наличием аналогичного болевого синдрома без использования комплекса фитосборов в процессе реабилитации. Комплексная терапия из фитосборов принималась внутрь. Больные основной группы после завершения курса лечения отмечали значительное субъективное улучшение, коррелирующее с результатами клинического объективного осмотра и с данными шкал. Достоинством данного метода является отсутствие побочных действий и осложнений, возможность применения как в стационаре, так и в амбулаторных условиях, как в процессе планового курса лечения, так и для достижения поддерживающего эффекта в периоды ремиссий

N-HOUSE STAFF TRAINING

The article deals with in-house staff training, its types and forms. The best form of training of own employees is definedВ статье рассмотрено внутрифирменное обучение персонала, его виды и формы. Определена наилучшая форма обучения собственных сотрудников

Genetic diversity of the Central European wild boar (Sus scrofa scrofa) population and domestic pig (Sus scrofa domesticus) breeds based on a microsatellite DNA locus

The results of studies of the genetic structure of the Central European wild boar (Sus scrofa scrofa) population and four breeds of domestic pigs (Duroc, Yorkshire, Large White and Landrace) bred in the Central Black Earth region of Russia are presented in this work. Based on 12 microsatellite loci, a significant ( p <0.05) decrease in the level of genetic variability in bred breeds was shown. The expected heterozygosity and Shannon index were as follows: in the wild boar, Ho = 0.763 ± 0.026, I = 1.717 ± 0.091; in the Duroc breed, Ho = 0.569 ± 0.068, I = 1.191 ± 0.157; in the Landrace, Ho = 0.618 ± 0.062, I = 1.201 ± 0.147; in the Large White, Ho = 0.680 ± 0.029, I = 1.362 ± 0.074; and in the Yorkshire, Ho = 0.642 ± 0.065, I = 1.287 ± 0.156. The results of checking genotypic Hardy–Weinberg equilibrium based on the G-test of maximum likelihood demonstrated that the overwhelming majority of loci in the wild boar population were in the state of said equilibrium. By contrast, in pig breed populations, some loci demonstrated a significant deviation from the indicated equilibrium. In addition, the Yorkshire, Large White, and Landrace populations had loci, for which the hypothesis of neutrality was reliably rejected based on the results of the Ewens–Watterson test. The revealed private alleles, characteristic of the wild boar and breeds, can later be used to identify them. The ordination of the centroids of different herds in the space of the first two principal coordinates based on the matrix of pairwise estimates of Nei’s genetic distances showed that the most distant populations are the Duroc and Boar breeds, and the most genetically close are the Yorkshire and Landrace breeds. The closest to the wild boar population was the Large White breed. The assessment of the effective size, carried out using the method based on the linkage disequilibrium and the molecular coancestry method, showed that in all studied groups, including the wild boar population, the effective size was less than 100 individuals. The low effective size of the wild boar population (Ne = 21.8, Neb = 4.0) is probably caused by the death and shooting of animals due to Pestis africana suum

Binase Immobilized on halloysite nanotubes exerts enhanced cytotoxicity toward human colon adenocarcinoma cells

© 2017 Khodzhaeva, Makeeva, Ulyanova. Many ribonucleases (RNases) are considered as promising tools for antitumor therapy because of their selective cytotoxicity toward cancer cells. Binase, the RNase from Bacillus pumilus, triggers apoptotic response in cancer cells expressing RAS oncogene which is mutated in a large percentage of prevalent and deadly malignancies including colorectal cancer. The specific antitumor effect of binase toward RAS-transformed cells is due to its direct binding of RAS protein and inhibition of downstream signaling. However, the delivery of proteins to the intestine is complicated by their degradation in the digestive tract and subsequent loss of therapeutic activity. Therefore, the search of new systems for effective delivery of therapeutic proteins is an actual task. This study is aimed to the investigation of antitumor effect of binase immobilized on natural halloysite nanotubes (HNTs). Here, we have developed the method of binase immobilization on HNTs and optimized the conditions for the enzyme loading and release (i); we have found the non-toxic concentration of pure HNTs which allows to distinguish HNTs- and binase-induced cytotoxic effects (ii); using dark-field and fluorescent microscopy we have proved the absorption of binase-loaded HNTs on the cell surface (iii) and demonstrated that binase-halloysite nanoformulations possessed twice enhanced cytotoxicity toward tumor colon cells as compared to the cytotoxicity of binase itself (iv). The enhanced antitumor activity of biocompatible binase-HNTs complex confirms the advisability of its future development for clinical practice