Johannesburg inner city’s appropriated buildings: resident’s responses to vulnerability and precarious living conditions

Thesis is submitted in partial fulfilment for the degree of Master of the Built Environment (Housing), to the Faculty of Engineering and the Built Environment, School of Architecture and Planning at the University of the Witwatersrand, Johannesburg, 2017Johannesburg like many rapidly urbanising cities around the world has the problem of a lack of affordable accommodation and inadequate access to basic services (Tissington, 2013). Residents in the inner city use spaces and buildings in a way that reclaims the promises of the city to a better life. As historian and cultural theorist Abdou Maliq Simone (2004) has noted people within African Cities have a probable tendency to improvise. In this research I use the concept of evolutionary resilience, which has been described to account for individuals and households ability adapt in constantly changing environments (Simmie & Martin, 2010) to explore the responses of residents to precarious living conditions and vulnerability that is created by conditions of insecure tenure and evictions. There is little comparative empirical research about how inner city residents talk about their lives and experiences. This research contributes to filling this gap by examining the experiences of residents and highlighting the ways in which as Cirugeda (2004) points to, residents often use empowerment strategies that encourage inhabitants to subvert laws and regulations, in order to maximise self-help by appropriating structures for better living conditions (Cirugeda 2004). This research utilises in depth interviews that were conducted within selected buildings in the inner city using a semi structured interview guide. The objective is to examine the strategies of coping with the exposure to risk and how individuals respond to these shocks. Şoitu (undated) states that vulnerability is a situation of social, economic and physiological need when individuals are marginalised and resilience is a personal resource that allows individuals to face stress and shocks and provides strength (Şoitu, undated). This research finds that there are many difficulties, threats and vulnerabilities that residents are exposed to and residents invoke various strategies and responses for coping. KEYWORDS ‘Bad buildings’, inner city, Johannesburg, vulnerability, evolutionary resilience, precarious living conditions, basic services, insecure tenureXL201

Law Enforcement Against Criminal Acts Of Cockfighting Gambling

This study aims to determine law enforcement against perpetrators of cockfighting gambling as a crime in the Gorontalo Regency area. The method used in this research is empirical or sociological research methodology. This study uses a qualitative approach and sampling using purposive sampling. The results of this study indicate that the process of law enforcement by the police against perpetrators of criminal acts of cockfighting gambling in the Gorontalo Regency area is carried out in two ways, namely first, pre-emptive law enforcement where in law enforcement the police take a persuasive approach to the community and provide direction on legal awareness in the environment. the community and also carry out mapping so that every crime event can be detected as early as possible. While the second law enforcement is repressive enforcement which is the final step taken by the Gorontalo Resort police to eradicate the practice of cockfighting gambling in Gorontalo Regency. This process consists of several stages; accept fights, investigations, investigations, arrests, confiscation of evidence, and detention. It is better not only to use the applicable criminal law as the overall solution, but also to take an integral or systemic approach. So, the effort to overcome this cockfighting gambling process must also be taken with a socio-cultural approach, a moralist approach, and a flexible educational approach to the current developments of society

Social Expenditure in New Zealand: Stochastic Projections

This paper presents stochastic projections for 13 categories of social spending in New Zealand over the period 2011-2061. These projections are based on detailed demographic estimates covering fertility, migration and mortality disaggregated by single year of age and gender. Distributional parameters are incorporated for all of the major variables, and are used to build up probabilistic projections for social expenditure as a share of GDP using simulation methods, following Creedy and Scobie (2005). Emphasis is placed on the considerable uncertainty involved in projecting future expenditure levels

SEKOLAH MENENGAH ATAS ISLAM TERPADU KOTA GORONTALO

ABSTRACTGorontalo City is the capital of Gorontalo Province which has a philosophy of “Adat Bersendikan Syarak, Syarak Bersendikan Kitabullah”. Which is marked by all operations in various fields that apply Islamic values. One of them is in the fields of education and architecture. The education mentioned is a combination of the learning system regulated in the national curriculum based on Islamic values and teachings, so that it’s implementation is carried out with an approach of combining general aducation and religious education into a curriculum fabric. Educational institutions or schools that implement this education system are called Integrated Islamic Schools. What is meant by Islamic values in architecture is a design process based on Islamic principles that produce a religious experience for it’s users; a process of human servitude to God. This study aimed to examine more deeply the Integrated Islamic School and Islamic Architecture, the values contained and their application, through literature review and observation. The results showed that Gorontalo City does not yet have a high school with an integrated education system, so it is necessary to have an Integrated Islamic Senior High School to City. Furthermore, school construction uses the concept of Islamic architecture with the aim of bringing Islamic values to life in the educational environment. This concept is expected to produce students who not only have qualified science and technology but also have faith and piety. Keywords: Schools, Islamic Architecture, Integrated Curriculum ABSTRAKKota Gorontalo adalah ibukota Provinsi Gorontalo yang merupakan daerah dengan falsafah “Adat Bersendikan Syarak, Syarak Bersendikan Kitabullah”. Hal ini juga ditandai dengan seluruh penyelenggaraan di berbagai bidang yang di dalamnya menerapkan nilai-nilai keislaman. Salah satu penyelenggaraan tersebut yaitu di bidang pendidikan dan arsitektur. Pendidikan yang dimaksud adalah suatu perpaduan antara sistem pembelajaran yang diatur dalam kurikulum nasional dengan kurikulum yang berbasis nilai dan ajaran Islam, sehingga penerapannya dilaksanakan dengan pendekatan penyelenggaraan dengan memadukan pendidikan umum dan pendidikan agama menjadi suatu jalinan kurikulum. Lembaga pendidikan atau sekolah yang menerapkan sistem pendidikan ini disebut dengan Sekolah Islam Terpadu (SIT). Adapun yang dimaksud dengan nilai-nilai keislaman di dalam arsitektur yaitu suatu proses desain berdasarkan kaidah-kaidah Islam yang memberikan hasil berupa pengalaman religius kepada penggunanya yang merupakan proses penghambaan diri seorang manusia kepada Tuhannya. Penelitian ini bertujuan untuk mengkaji lebih dalam lagi tentang Sekolah Islam Terpadu dan Arsitektur Islam, nilai yang terkandung dan penerapannya, melalui kajian literatur dan pengamatan. Hasil penelitian menunjukkan bahwa Kota Gorontalo belum memiliki Lembaga Pendidikan Menengah Atas dengan sistem pendidikan terpadu, sehingga perlu adanya Sekolah Menengah Atas Islam Terpadu (SMAIT) untuk melengkapi proses jenjang pembelajaran Sekolah Islam Terpadu yang telah tersedia (SDIT dan SMPIT) di kota Gorontalo, kemudian bangunan Sekolah Menengah Atas Islam Terpadu tersebut menggunakan konsep Arsitektur Islam dengan tujuan agar lebih menghidupkan nilai-nilai keislaman di lingkungan pendidikan. Konsep ini diharapkan dapat menghasilkan siswa dan siswi yang tidak hanya memiliki ilmu pengetahuan dan teknologi yang mumpuni, tetapi juga mempunyai iman dan takwa yang mapan. Kata Kunci: Sekolah, Arsitektur Islam, Kurikulum Terpad

The Dantu blood group prevents parasite growth in vivo: Evidence from a controlled human malaria infection study

Background: The long co-evolution of Homo sapiens and Plasmodium falciparum has resulted in the selection of numerous human genetic variants that confer an advantage against severe malaria and death. One such variant is the Dantu blood group antigen, which is associated with 74% protection against severe and complicated P. falciparum malaria infections in homozygous individuals, similar to that provided by the sickle haemoglobin allele (HbS). Recent in vitro studies suggest that Dantu exerts this protection by increasing the surface tension of red blood cells, thereby impeding the ability of P. falciparum merozoites to invade them and reducing parasite multiplication. However, no studies have yet explored this hypothesis in vivo. Methods: We investigated the effect of Dantu on early phase P. falciparum (Pf) infections in a controlled human malaria infection (CHMI) study. 141 sickle-negative Kenyan adults were inoculated with 3.2 × 103 aseptic, purified, cryopreserved Pf sporozoites (PfSPZ Challenge) then monitored for blood-stage parasitaemia for 21 days by quantitative polymerase chain reaction (qPCR)analysis of the 18S ribosomal RNA P. falciparum gene. The primary endpoint was blood-stage P. falciparum parasitaemia of ≥500/μl while the secondary endpoint was the receipt of antimalarial treatment in the presence of parasitaemia of any density. On study completion, all participants were genotyped both for Dantu and for four other polymorphisms that are associated with protection against severe falciparum malaria: α+-thalassaemia, blood group O, G6PD deficiency, and the rs4951074 allele in the red cell calcium transporter ATP2B4. Results: The primary endpoint was reached in 25/111 (22.5%) non-Dantu subjects in comparison to 0/27 (0%) Dantu heterozygotes and 0/3 (0.0%) Dantu homozygotes (p=0.01). Similarly, 49/111 (44.1%) non-Dantu subjects reached the secondary endpoint in comparison to only 7/27 (25.9%) and 0/3 (0.0%) Dantu heterozygotes and homozygotes, respectively (p=0.021). No significant impacts on either outcome were seen for any of the other genetic variants under study. Conclusions: This study reveals, for the first time, that the Dantu blood group is associated with high-level protection against early, non-clinical, P. falciparum malaria infections in vivo. Learning more about the mechanisms involved could potentially lead to new approaches to the prevention or treatment of the disease. Our study illustrates the power of CHMI with PfSPZ Challenge for directly testing the protective impact of genotypes previously identified using other methods

Characterization of a human tumorsphere glioma orthotopic model using magnetic resonance imaging

Magnetic resonance imaging (MRI) is the imaging modality of choice by which to monitor patient gliomas and treatment effects, and has been applied to murine models of glioma. However, a major obstacle to the development of effective glioma therapeutics has been that widely used animal models of glioma have not accurately recapitulated the morphological heterogeneity and invasive nature of this very lethal human cancer. This deficiency is being alleviated somewhat as more representative models are being developed, but there is still a clear need for relevant yet practical models that are well-characterized in terms of their MRI features. Hence we sought to chronicle the MRI profile of a recently developed, comparatively straightforward human tumor stem cell (hTSC) derived glioma model in mice using conventional MRI methods. This model reproduces the salient features of gliomas in humans, including florid neoangiogenesis and aggressive invasion of normal brain. Accordingly, the variable, invasive morphology of hTSC gliomas visualized on MRI duplicated that seen in patients, and it differed considerably from the widely used U87 glioma model that does not invade normal brain. After several weeks of tumor growth the hTSC model exhibited an MRI contrast enhancing phenotype having variable intensity and an irregular shape, which mimicked the heterogeneous appearance observed with human glioma patients. The MRI findings reported here support the use of the hTSC glioma xenograft model combined with MRI, as a test platform for assessing candidate therapeutics for glioma, and for developing novel MR methods

Genetic determinants of glucose-6-phosphate dehydrogenase activity in Kenya

Background: The relationship between glucose-6-phosphate dehydrogenase (G6PD) deficiency and clinical phenomena such as primaquine-sensitivity and protection from severe malaria remains poorly defined, with past association studies yielding inconsistent and conflicting results. One possibility is that examination of a single genetic variant might underestimate the presence of true effects in the presence of unrecognized functional allelic diversity. Methods: We systematically examined this possibility in Kenya, conducting a fine-mapping association study of erythrocyte G6PD activity in 1828 Kenyan children across 30 polymorphisms at or around the G6PD locus. Results: We demonstrate a strong functional role for c.202G>A (rs1050828), which accounts for the majority of variance in enzyme activity observed (P=1.5 × 10-200, additive model). Additionally, we identify other common variants that exert smaller, intercorrelated effects independent of c.202G>A, and haplotype analyses suggest that each variant tags one of two haplotype motifs that are opposite in sequence identity and effect direction. We posit that these effects are of biological and possible clinical significance, specifically noting that c.376A>G (rs1050829) augments 202AG heterozygote risk for deficiency trait by two-fold (OR = 2.11 [1.12 - 3.84], P=0.014). Conclusions: Our results suggest that c.202G>A is responsible for the majority of the observed prevalence of G6PD deficiency trait in Kenya, but also identify a novel role for c.376A>G as a genetic modifier which marks a common haplotype that augments the risk conferred to 202AG heterozygotes, suggesting that variation at both loci merits consideration in genetic association studies probing G6PD deficiency-associated clinical phenotypes. </p

Effect of the JAK2/STAT3 inhibitor SAR317461 on human glioblastoma tumorspheres

BackgroundThe STAT3 transcription factor is a major intracellular signaling protein and is frequently dysregulated in the most common and lethal brain malignancy in adults, glioblastoma multiforme (GBM). Activation of STAT3 in GBM correlates with malignancy and poor prognosis. The phosphorylating signal transducer JAK2 activates STAT3 in response to cytokines and growth factors. Currently there are no JAK-STAT pathway inhibitors in clinical trials for GBM, so we sought to examine the anti-GBM activity of SAR317461 (Sanofi-Aventis), a newer generation, highly potent JAK2 inhibitor that exhibits low toxicity and good pharmacokinetics. SAR317461 was initially approved for patient testing in the treatment of primary myelofibrosis (PMF), and has shown activity in preclinical models of melanoma and pulmonary cancer, but has not been tested in GBM.MethodsWe hypothesized that a potent small molecule JAK2 inhibitor could overcome the heterogeneous nature of GBM, and suppress a range of patient derived GBM tumorsphere lines and immortalized GBM cell lines. We treated with SAR317461 to determine IC50 values, and using Western blot analysis we asked whether the response was linked to STAT3 expression. Western blot analysis, FACS, and cell viability studies were used to identify the mechanism of SAR317461 induced cell death.ResultsWe report for the first time that the JAK2 inhibitor SAR317461 clearly inhibited STAT3 phosphorylation and had substantial activity against cells (IC50 1-10&nbsp;µM) from 6 of 7 different patient GSC derived GBM tumorsphere lines and three immortalized GBM lines. One patient GSC derived line did not constitutively express STAT3 and was more resistant to SAR317461 (IC50 ≈25&nbsp;µM). In terms of mechanism we found cleaved PARP and clear apoptosis following SAR317461. SAR317461 also induced autophagy and the addition of an autophagy inhibitor markedly enhanced cell killing by SAR317461.ConclusionsWe conclude that SAR317461 potently inhibits STAT3 phosphorylation and that it has significant activity against those GBM cells which express activated STAT3. Further studies are warranted in terms of the potential of SAR317461 as single and combined therapy for selectively treating human patients afflicted with GBMs expressing activation of the JAK2-STAT3 signaling axis

β-Thalassemia pathogenic variants in a cohort of children from the East African coast

BACKGROUND: β-Thalassemia is rare in sub-Saharan Africa. Previous studies have suggested that it is limited to specific parts of West Africa. Based on hemoglobin A2 (HbA2 ) concentrations measured by HPLC, we recently speculated that β-thalassemia might also be present on the East African coast of Kenya. Here, we follow this up using molecular methods. METHODS: We used raised hemoglobin A2 (HbA2 ) values (> 4.0% of total Hb) to target all HbAA members of a cohort study in Kilifi, Kenya, for HBB sequencing for β-thalassemia (n = 99) together with a sample of HbAA subjects with lower HbA2 levels. Because HbA2 values are artifactually raised in subjects carrying sickle hemoglobin (HbS) we sequenced all participants with an HPLC pattern showing HbS without HbA (n = 116) and a sample with a pattern showing both HbA and HbS. RESULTS: Overall, we identified 83 carriers of four separate β-thalassemia pathogenic variants: three β0 -thalassemia [CD22 (GAA→TAA), initiation codon (ATG→ACG), and IVS1-3' end del 25bp] and one β+ -thalassemia pathogenic variants (IVS-I-110 (G→A)). We estimated the minimum allele frequency of all variants combined within the study population at 0.3%. CONCLUSIONS: β-Thalassemia is present in Kilifi, Kenya, an observation that has implications for the diagnosis and clinical care of children from the East Africa region

Gastrointestinal effects associated with soluble and insoluble copper in drinking water.

The aim of this study was to determine whether total copper or soluble copper concentration is associated with gastrointestinal signs and symptoms. Forty-five healthy adult women (18-55 years of age), living in Santiago, Chile, ingested tap water with 5 mg/L of copper containing different ratios of soluble copper (copper sulfate) and insoluble copper (copper oxide) over a 9-week period. Three randomized sequences of the different copper ratios (0:5, 1:4, 2:3, 3:2, and 5:0 mg/L) were followed. Subjects recorded their water consumption and gastrointestinal symptoms daily on a special form. Mean water consumption was similar among groups. Serum copper levels, ceruloplasmin, and activities of liver enzymes were within normal limits. No differences were detected between the means of biochemical parameters at the beginning and at the end of the study. Twenty subjects presented gastrointestinal disturbances at least once during the study, 9 suffered diarrhea (with or without abdominal pain and vomiting), and the other 11 subjects reported abdominal pain, nausea, or vomiting. No differences were found in incidence of abdominal pain, nausea, vomiting, and diarrhea regardless of the ratio of copper sulfate to copper oxide. In conclusion, both copper sulfate (a soluble compound) and copper oxide (an insoluble compound) have comparable effects on the induction of gastrointestinal manifestations, implying that similar levels of ionic copper were present in the stomach