Mortality of fish fry as a result of specific and aspecific cercarial invasion under experimental conditions

Although the effect exerted on fish by fluke cercariae developing in fish as intermediate hosts is fairly well known, cercariae of flukes requiring intermediate hosts other than fish occur in fish ponds in much higher numbers. To study the effect of these latter cercariae on fish fry, a few days old common carp fry (Cyprinus carpio) were exposed to cohorts of xiphidiocercariae or echinocercariae shed by one or two snails acting as intermediate hosts. The observed pathogenicity of these cercariae was compared to the well-known pathogenic effect ofDiplostomumcercariae. AlthoughDiplostomumcercariae proved to be the most pathogenic, occasionally also the other two types of cercariae could kill the common carp fry by invading their body. Xiphidiocercariae ingested by the fish penetrated the intestinal wall and developed into metacercariae within the body of the fry. They could also get into the musculature through the skin. The much larger echinocercariae invaded the gill cavity where they became encapsulated, thus hampering gill function and leading to fish mortality due to oxygen deficiency.Diplostomumcercariae were more pathogenic than the cercariae of the other two flukes in that they were shed by the intermediate host in very large numbers. Although much less xiphidiocercariae and echinocercariae emerged from the intermediate host snails thanDiplostomumcercariae, the former could kill the fish fry already in lower numbers. These findings prove that fluke cercariae nonspecific to fish may exert pathogenic effects on fish, and presumably contribute to the early mortality of young fry placed out into fish ponds

First autochthonous Dirofilaria immitis (Leidy, 1856) infection in a dog in Hungary

AbstractA 4 year-old, male Hungarian Vizsla dog which had never been abroad was referred with poor general condition, decrease in body weight, haematemesis and jaundice to the Central Clinic of Veterinary Science University, Budapest. After symptomatic treatment abdominal ultrasonography and diagnostic laparatomy were carried out. The dog was humanely euthanized two days later following owner's consent because of sudden worsening of clinical conditions. Two adult heartworms (Dirofilaria immitis) were found in the right ventricle partially coiling around the tricuspid valve. PCR on blood was positive for both D. immitis and Dirofilaria repens while only D. repens microfilariae were found by modified Knott's test and the serological test was negative for D. immitis antigens. This is the first, confirmed report of autochthonous canine heartworm infection in Hungary

The Bean-Livingston barrier at a superconductor/magnet interface

The Bean-Livingston barrier at the interface of type-II superconductor/soft-magnet heterostructures is studied on the basis of the classical London approach. This shows a characteristic dependence on the geometry of the particular structure and its interface as well as on the relative permeability of the involved magnetic constituent. The modification of the barrier by the presence of the magnet can be significant, as demonstrated for a cylindrical superconducting filament covered with a coaxial magnetic sheath. Using typical values of the relative permeability, the critical field of first penetration of magnetic flux is predicted to be strongly enhanced, whereas the variation of the average critical current density with the external field is strongly depressed, in accord with the observations of recent experiments.Comment: RevTeX 4; revised version; accepted in Journal of Physics: Condensed Matte

The role of p53 in the DNA damage-related ubiquitylation of S2P RNAPII

DNA double-strand breaks are one of the most deleterious lesions for the cells, therefore understanding the macromolecular interactions of the DNA repair-related mechanisms is essential. DNA damage triggers transcription silencing at the damage site, leading to the removal of the elongating RNA polymerase II (S2P RNAPII) from this locus, which provides accessibility for the repair factors to the lesion. We previously demonstrated that following transcription block, p53 plays a pivotal role in transcription elongation by interacting with S2P RNAPII. In the current study, we reveal that p53 is involved in the fine-tune regulation of S2P RNAPII ubiquitylation. Furthermore, we emphasize the potential role of p53 in delaying the premature ubiquitylation and the subsequent chromatin removal of S2P RNAPII as a response to transcription block

Niobium based intermetallics as a source of high-current/high-magnetic field superconductors

The article is focused on low temperature intermetallic A15 superconducting wires development for Nuclear Magnetic Resonance, NMR, and Nuclear Magnetic Imaging, MRI, magnets and also on cryogen-free magnets. There are many other applications which would benefit from new development such as future Large Hadron Collider to be built from A15 intermetallic conductors. This paper highlights the current status of development of the niobium based intermetallics with special attention to Nb 3 (Al 1-x, Ge x). Discussion is focused on the materials science aspects of conductor manufacture, such as b-phase (A15) formation, with particular emphasis on the maximisation of the superconducting parameters, such as critical current density, Jc, critical temperature, Tc, and upper critical field, Hc2 . Many successful manufacturing techniques of the potential niobium-aluminide intermetallic superconducting conductors, such as solid-state processing, liquid-solid processing, rapid heating/cooling processes, are described, compared and assessed. Special emphasis has been laid on conditions under which the Jc (B) peak effect occurs in some of the Nb3(Al,Ge) wires. A novel electrodeoxidizing method developed in Cambridge whereby the alloys and intermetallics are produced cheaply making all superconducting electromagnetic devices, using low cost LTCs, more cost effective is presented.This new technique has potential to revolutionise the existing superconducting industry enabling reduction of cost orders of magnitude.Comment: Paper presented at EUCAS'01 conference, Copenhagen, 26-30 August 200

Angiostrongylosis-related restrictive pneumopathy assessed by arterial blood gas analysis in a dog

Pulmonary angiostrongylosis was diagnosed by the Baermann method and larval identification from faecal and bronchoalveolar lavage samples in a five-month- old male mongrel dog with dyspnoea and cough. Arterial blood gas analysis indicated arterial hypoxaemia and restrictive pneumopathy. In addition to the palliative treatment, fenbendazole was administered (50 mg/kg/24 h per os) for 14 days. The respiratory signs subsided within a short time clinically, but serial arterial blood gas analysis demonstrated an ongoing ventilation disorder. Repeated haematology, thoracic radiography, bronchoscopy and blood gas analysis were performed to follow the course of the disease. The most severe eosinophilia was detected after the beginning of the anthelmintic therapy, and the arterial pO2 level was permanently low. Arterial blood gas analysis provided the most adequate information about the course of the pneumopathy and it greatly facilitated the patient’s medical management

Modeling the Evolution of Regulatory Elements by Simultaneous Detection and Alignment with Phylogenetic Pair HMMs

The computational detection of regulatory elements in DNA is a difficult but important problem impacting our progress in understanding the complex nature of eukaryotic gene regulation. Attempts to utilize cross-species conservation for this task have been hampered both by evolutionary changes of functional sites and poor performance of general-purpose alignment programs when applied to non-coding sequence. We describe a new and flexible framework for modeling binding site evolution in multiple related genomes, based on phylogenetic pair hidden Markov models which explicitly model the gain and loss of binding sites along a phylogeny. We demonstrate the value of this framework for both the alignment of regulatory regions and the inference of precise binding-site locations within those regions. As the underlying formalism is a stochastic, generative model, it can also be used to simulate the evolution of regulatory elements. Our implementation is scalable in terms of numbers of species and sequence lengths and can produce alignments and binding-site predictions with accuracy rivaling or exceeding current systems that specialize in only alignment or only binding-site prediction. We demonstrate the validity and power of various model components on extensive simulations of realistic sequence data and apply a specific model to study Drosophila enhancers in as many as ten related genomes and in the presence of gain and loss of binding sites. Different models and modeling assumptions can be easily specified, thus providing an invaluable tool for the exploration of biological hypotheses that can drive improvements in our understanding of the mechanisms and evolution of gene regulation

Diffusion of Alexa Fluor 488-Conjugated Dendrimers in Rat Aortic Tissue

In this study, the distribution of labeled dendrimers in native and aneurysmal rat aortic tissue was examined. Adult male rats underwent infrarenal aorta perfusion with generation 5 (G5) acetylated Alexa Fluor 488-conjugated dendrimers for varying lengths of time. In a second set of experiments, rats underwent aortic elastase perfusion followed by aortic dendrimer perfusion 7 days later. Aortic diameters were measured prior to and postelastase perfusion, and again on the day of harvest. Aortas were harvested 0, 12, or 24 h postperfusion, fixed, and mounted. Native aortas were harvested and viewed as negative controls. Aortic cross-sections were viewed and imaged using confocal microscopy. Dendrimers were quantified (counts high-powered field). Results were evaluated by repeated measures ANOVA and Student's t -test. We found that in native aortas, dendrimers penetrated the aortic wall in all groups. For all perfusion times, fewer dendrimers were present as time between dendrimer perfusion and aortic harvest increased. Longer perfusion times resulted in increased diffusion of dendrimers throughout the aortic wall. By 24 h, the majority of the dendrimers were through the wall. Dendrimers in aneurysmal aortas, on day 0 postdendrimer perfusion, diffused farther into the aortic wall than controls. In conclusion, this study documents labeled dendrimers delivered intra-arterially to native rat aortas in vivo , and the temporal diffusion of these molecules within the aortic wall. Increasing perfusion time and length of time prior to harvest resulted in continued dendrimer diffusion into the aortic wall. These preliminary data provide a novel mechanism whereby local inhibitory therapy may be delivered locally to aortic tissue.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72448/1/annals.1383.004.pd

SerpinB10, a Serine Protease Inhibitor, Is Implicated in UV-Induced Cellular Response

UV-induced DNA damage response and repair are extensively studied processes, as any malfunction in these pathways contributes to the activation of tumorigenesis. Although several proteins involved in these cellular mechanisms have been described, the entire repair cascade has remained unexplored. To identify new players in UV-induced repair, we performed a microarray screen, in which we found SerpinB10 (SPB10, Bomapin) as one of the most dramatically upregulated genes following UV irradiation. Here, we demonstrated that an increased mRNA level of SPB10 is a general cellular response following UV irradiation regardless of the cell type. We showed that although SPB10 is implicated in the UV-induced cellular response, it has no indispensable function in cell survival upon UV irradiation. Nonetheless, we revealed that SPB10 might be involved in delaying the duration of DNA repair in interphase and also in S-phase cells. Additionally, we also highlighted the interaction between SPB10 and H3. Based on our results, it seems that SPB10 protein is implicated in UV-induced stress as a “quality control protein”, presumably by slowing down the repair process