Purification and partial characterization of serum immunoglobulin from Persian sturgeon (Acipenser persicus)

In this study, immunoglobulins from serum of Persian sturgeon (Acipenser persicus) were purified and partially characterized. Immunoglobulins were purified from the pooled sera by a combination of salt precipitation, Ion-exchange chromatography and gel filtration methods. DEAE sepharosefast flow and sepharose CL-6B columns were used for Ion exchange-chromatography, and gel filtration, respectively. The purity, molecular weight and molecular distribution of the immunoglobulin preparations was determined by gel electrophoresis (SDS-P AGE) in reducing and non-reducing situations. In gel filtrated immunoglobulins two distinct peaks, high molecular weight (HMW Ig) and low molecular weight (LMW Ig) were obtained. Both HMW Ig and LMW Ig had identical heavy and light chains of 72-75 KDa and 27-29 KDa, respectively, in reducing SDS-P AGE. HMW Ig contained a group of bands, including two major bands in non-reducing SDS-PAGE, In contrast LMW Ig contain more than half of the total immunoglobulin, was 190 KDa. In ion exchange chromatography, immunoglobulins were eluted in three peaks. The first was exclusively monomer and others were mixture of monomer and polymers. This is the first report on persian sturgeon immunoglobulins. Results of this investigation showed that persian sturgeon immunoglobulins was not homogenous in respect of molecular distribution, PI and the type of light chain. The presence of more than one genes for light and\or heavy chains or post transcriptional and\or post modifications may be responsible for these variations

Association of diverse bacterial communities in human bile samples with biliary tract disorders: a survey using culture and polymerase chain reaction-denaturing gradient gel electrophoresis methods

Bacterial infection is considered a predisposing factor for disorders of the biliary tract. This study aimed to determine the diversity of bacterial communities in bile samples and their involvement in the occurrence of biliary tract diseases. A total of 102 bile samples were collected during endoscopic retrograde cholangiopancreatography (ERCP). Characterization of bacteria was done using culture and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) methods. Antimicrobial susceptibility of the isolates was determined based on the Clinical and Laboratory Standards Institute (CLSI) guidelines and identity of the nucleotide sequences of differentiated bands from the DGGE gels was determined based on GenBank data. In total, 41.2 (42/102) of the patients showed bacterial infection in their bile samples. This infection was detected in 21 (4/19), 45.4 (5/11), 53.5 (15/28), and 54.5 (24/44) of patients with common bile duct stone, microlithiasis, malignancy, and gallbladder stone, respectively. Escherichia coli showed a significant association with gallstones. Polymicrobial infection was detected in 48 of the patients. While results of the culture method established coexistence of biofilm-forming bacteria (Pseudomonas aeruginosa, E. coli, Klebsiella pneumoniae, Enterococcus spp., and Acinetobacter spp.) in different combinations, the presence of Capnocytophaga spp., Lactococcus spp., Bacillus spp., Staphylococcus haemolyticus, Enterobacter or Citrobacter spp., Morganella spp., Salmonella spp., and Helicobacter pylori was also characterized in these samples by the PCR-DGGE method. Multidrug resistance phenotypes (87.5 ) and resistance to third- and fourth-generation cephalosporins and quinolones were common in these strains, which could evolve through their selection by bile components. Ability for biofilm formation seems to be a need for polymicrobial infection in this organ. © 2016 Springer-Verlag Berlin Heidelber

Industrial waste management in Shokuhieh industrial zone of Qom province in 2013

Background and aims: Nowadays, the rapid growth of industries and production of various products has led to an increase in hazardous industrial solid waste. The aim of this study was to examine how to manage industrial solid waste in industrial zone of Shokuhiyeh in Qom province in 2013. Methods: This research was a descriptive, cross-sectional one. The data were gathered by first referring to industrial units and the completion of Iranian Environmental Organization Questionnaire and then analyzing the gathered data. This questionnaire included 24 items covering the quantity, quality, and management of industrial solid waste. In this study, 41 industrial units with personnel of more than 50 were examined. Results: The category of" chemical and metal industries" produced the highest solid waste and specific solid waste. The elements making up the produced solid waste were plastic 38% and glass 27.5%, respectively. Among the storage methods, the temporary solid waste storage in baskets 36% and open-doors method 32% were the most common ones. 41% of industrial units disposed of solid waste monthly, 31.7% weekly, 24.4% daily and 2.4% in an inconsistent manner. 58% of produced solid waste was recycled, 35% was landfilled, 4.6% was burned up, and 2.4% was disposed of in an unknown manner. Conclusion: The results of this study showed that the collection and storage of industrial solid waste in the industrial zone of Shokuhiyeh is not done in a suitable manner. Moreover, no educational program has yet been done about the management of solid waste in most of the industries. Therefore, it seems indispensable to establish a suitable system of solid waste management in order to organize the conditions of industrial solid waste produced in this zone

Burden of ischemic heart diseases in Iran, 1990-2010: Findings from the global burden of disease study 2010

Background: Cardiovascular diseases are viewed worldwide as one of the main causes of death.This study aims to report the burden of ischemic heart diseases (IHDs) in Iran by using data of the global burden of disease (GBD) study, 1990-2010. Materials and Methods: The GBD study 2010 was a systematic effort to provide comprehensive data to calculate disability-adjusted life years (DALYs) for diseases and injuries in the world. Years of life lost (YLLs) due to premature mortality were computed on the basis of cause-of-death estimates, using Cause of Death Ensemble model (CODEm). Years lived with disability (YLDs) were assessed by the multiplication of prevalence, the disability weight for a sequel, and the duration of symptoms. A systematic review of published and unpublished data was performed to evaluate the distribution of diseases, and consequently prevalence estimates were calculated with a Bayesian meta-regression method (DisMod-MR). Data from population-based surveys were used for producing disability weights. Uncertainty from all inputs into the calculations of DALYs was disseminated by Monte Carlo simulation techniques. Results: The age-standardized IHDs DALY specified rate decreased 31.25 over 20 years from 1990 to 2010 from 4720 (95% uncertainty interval (UI): 4,341-5,099) to 3,245 (95% UI: 2,810-3,529) person-years per 100,000. The decrease were 38.14% among women and 26.87% among men. The age-standardized IHDs death specefied rate decreased by 21.17% from 222) 95% UI: 207-243 (to 175 (95% UI:152-190) person-years per 100,000 in both the sexes. The age-standardized YLL and YLD rates decreased 32.05% and 4.28%, respectively, in the above period. Conclusion: Despite decreasing age-standardized IHD of mortality, YLL, YLD, and DALY rates from 1990 to 2010, population growth and aging increased the global burden of IHD. YLL has decreased more than IHD deaths and YLD since 1990 but IHD mortality remains the greatest contributor to disease burden. © 2015 Journal of Research in Medical Sciences

Frontiers in conventional and nanomaterials based electrochemical sensing and biosensing approaches for Ochratoxin A analysis in foodstuffs: A review

Highly sensitive and rapid methods for the Ochratoxin A (OTA) detection as a main class of Ochratoxins (OT) in various food samples is significant. In the present review, novel OTA detection techniques based on electrochemical and conventional methods have been extensively explained, and also compared with each other in food samples. We classified the various electrochemical based techniques of OTA determination creatively into Impedimetric, Voltammetric, Amperometric and Electrochemiluminescence assays and their efficient comparison with conventional, and clinical methods including Capillary Electrophoresis (CE), High Performance Liquid Chromatography (HPLC), Liquid Chromatography/Mass Spectroscopy (LC/MS), and Enzyme Linked Immunosorbent Assay (ELISA) has been performed comprehensively. Moreover, structure, characteristics, and different types of OT and OTA are explained as extensively as possible. A summary of the current limitations and future challenges in OTA analysis are described, which efficiently provide a pathway for further developments and applications. © 2021 Elsevier Lt

Patulin and Trichothecene: characteristics, occurrence, toxic effects and detection capabilities via clinical, analytical and nanostructured electrochemical sensing/biosensing assays in foodstuffs

Patulin and Trichothecene as the main groups of mycotoxins in significant quantities can cause health risks from allergic reactions to death on both humans and animals. Accordingly, rapid and highly sensitive determination of these toxics agents is of great importance. This review starts with a comprehensive outlook regarding the characteristics, occurrence and toxic effects of Patulin and Trichothecene. In the following, numerous clinical and analytical approaches have been extensively discussed. The main emphasis of this review is placed on the utilization of novel nanomaterial based electrochemical sensing/biosensing tools for highly sensitive determination of Patulin and Trichothecene. Furthermore, a detailed and comprehensive comparison has been performed between clinical, analytical and sensing methods. Subsequently, the nanomaterial based electrochemical sensing platforms have been approved as reliable tools for on-site analysis of Patulin and Trichothecene in food processing and manufacturing industries. Different nanomaterials in improving the performance of detecting assays were investigated and have various benefits toward clinical and analytical methods. This paper would address the limitations in the current developments as well as the future challenges involved in the successful construction of sensing approaches with the functionalized nanomaterials and also allow exploring into core-research works regarding this area. © 2021 Taylor & Francis Group, LLC