Bioenergetic/oxidative balance in feline morulae and blastocysts produced in vitro after temporary oocyte holding at room temperature versus cold ovary storage

A major problem in in vitro embryo production (IVEP) is the availability of methods for ovary or oocyte preservation and transport. The suitability of a given method can be assessed by analizing the bioenergetic/oxidative balance of obtained embryos, indicating oocyte/embryo health status during the whole IVEP chain. Unlike other species, cat oocytes have the unique ability to mature in vitro after cold ovary storage (COS; 1), which provides opportunities to rescue oocytes after ovariohysterectomy or sudden death. An alternative strategy is oocyte holding at room temperature (r.t.) in a medium without meiotic inhibitors, namely EH medium, as established in the mare (EH, 2). The aim of this study was to evaluate the effects of temporary (6h) oocyte holding at r.t. in EH medium versus COS on the development and bioenergetic/oxidative balance of feline late stage embryos, morulae (M) and blastocysts (Bl), obtained after in vitro maturation (IVM), fertilization (IVF) and embryo culture (IVEC). The study was conducted in autumn 2016 in 7 replicates. Ovaries were collected from 17 anestrous queens by routine ovariohysterectomy at the Veterinary Hospital of University of Bari. For each queen, one ovary underwent immediate slicing and retrieved cumulus-oocyte complexes (COCs) were placed at r.t. (22-27\ub0C) in the dark for 6h in EH medium (40% Earle\u2019s, 40% Hank\u2019s salts-buffered M199 and 20% FCS; 2), and the other ovary was placed in PBS solution and stored for 6h at 4\ub0C (1) until slicing. Only COCs with uniform, darkly pigmented ooplasm and intact cumulus (1) underwent 24h IVM in a TCM199-based medium at 38.5\ub0C under 5% CO2 in air (3). Fresh sperm cells were collected by flushing cauda epididymis of male cats, following routine orchiectomy. COCs and sperm cells (2x106/ml) were coincubated for 18-22h for IVF under 5% CO2 in air. IVEC lasted for 7 days under 5% CO2, 5% O2, 90% N2. IVF and IVEC were performed in SOF media (3). Embryo development was followed up to M and Bl stage and confirmed by nuclear chromatin evaluation. The bioenergetic/oxidative status of M and Bl was assessed by confocal microscopy after mitochondria and reactive oxygen species (ROS) labeling (4). Data were analysed by Chi-square and Student\u2019s t Test (Significance when P 640.05). A total of 359 oocytes were used, 185 after EH and 174 after COS. No differences were observed between the two groups for embryo cleavage (22/185, 12% vs 21/174, 12%; P>0.05), M/cleaved (4/22, 18% vs 8/21, 38%; P>0.05), Bl/cleaved (2/22, 9% vs 5/21, 24%; P>0.05) and number of nuclei (M: 39.5\ub130.4 vs 40\ub10; P>0.05; Bl: 100\ub114.1 vs 107.6\ub121.9; P>0.05). Compared with the EH group, COS-embryos showed mitochondria over-activity (arbitrary densitometry units, ADU: 38.2\ub115.0 vs 19.9\ub112.2; P<0.0001), increased ROS levels (ADU: 17.8\ub17.9 vs 10.9\ub14.1; P<0.0001) and significantly lower mitochondria/ROS colocalization (Pearson coefficient: 0.25\ub10.1 vs 0.34\ub10.2; P<0.001), overall indicating worse health conditions. We conclude that EH could be a valid alternative to COS for feline oocyte hortterm preservation, as it allowed to produce similar rates of M+Bl and these embryos showed improved bioenergetic/oxidative balance. [1]Wolfe & Wildt, J Reprod Fertil 1996;106:135-41. [2]Choi et al, Theriogenology 2006;66:955-63. [3]Martino et al, Reprod Toxicol 2016;65:204\u201311. [4]Martino et al, Reprod Biol Endocrinol 2013;11:27. [5]Gonzalez et al, Reprod Dom Anim 2012;47:118-2

Euclid: Early Release Observations -- Dwarf galaxies in the Perseus galaxy cluster

International audienceWe make use of the unprecedented depth, spatial resolution, and field of view of the Euclid Early Release Observations of the Perseus galaxy cluster to detect and characterise the dwarf galaxy population in this massive system. The Euclid high resolution VIS and combined VIS+NIR colour images were visually inspected and dwarf galaxy candidates were identified. Their morphologies, the presence of nuclei, and their globular cluster (GC) richness were visually assessed, complementing an automatic detection of the GC candidates. Structural and photometric parameters, including Euclid filter colours, were extracted from 2-dimensional fitting. Based on this analysis, a total of 1100 dwarf candidates were found across the image, with 638 appearing to be new identifications. The majority (96%) are classified as dwarf ellipticals, 53% are nucleated, 26% are GC-rich, and 6% show disturbed morphologies. A relatively high fraction of galaxies, 8%, are categorised as ultra-diffuse galaxies. The majority of the dwarfs follow the expected scaling relations. Globally, the GC specific frequency, S_N, of the Perseus dwarfs is intermediate between those measured in the Virgo and Coma clusters. While the dwarfs with the largest GC counts are found throughout the Euclid field of view, those located around the east-west strip, where most of the brightest cluster members are found, exhibit larger S_N values, on average. The spatial distribution of the dwarfs, GCs, and intracluster light show a main iso-density/isophotal centre displaced to the west of the bright galaxy light distribution. The ERO imaging of the Perseus cluster demonstrates the unique capability of Euclid to concurrently detect and characterise large samples of dwarfs, their nuclei, and their GC systems, allowing us to construct a detailed picture of the formation and evolution of galaxies over a wide range of mass scales and environments

Euclid: Early Release Observations -- A preview of the Euclid era through a galaxy cluster magnifying lens

International audienceWe present the first analysis of the Euclid Early Release Observations (ERO) program that targets fields around two lensing clusters, Abell 2390 and Abell 2764. We use VIS and NISP imaging to produce photometric catalogs for a total of $\sim 500\,000$ objects. The imaging data reach a $5\,\sigma$ typical depth in the range 25.1-25.4 AB in the NISP bands, and 27.1-27.3 AB in the VIS band. Using the Lyman-break method in combination with photometric redshifts, we identify $30$ Lyman-break galaxy (LBG) candidates at $z>6$ and 139 extremely red sources (ERSs), most likely at lower redshift. The deeper VIS imaging compared to NISP means we can routinely identify high-redshift Lyman breaks of the order of $3$ magnitudes, which reduces contamination by brown dwarf stars and low-redshift galaxies. Spectroscopic follow-up campaigns of such bright sources will help constrain both the bright end of the ultraviolet galaxy luminosity function and the quasar luminosity function at $z>6$, and constrain the physical nature of these objects. Additionally, we have performed a combined strong lensing and weak lensing analysis of A2390, and demonstrate how Euclid will contribute to better constraining the virial mass of galaxy clusters. From these data, we also identify optical and near-infrared counterparts of known $z>0.6$ clusters, which exhibit strong lensing features, establishing the ability of Euclid to characterize high-redshift clusters. Finally, we provide a glimpse of Euclid's ability to map the intracluster light out to larger radii than current facilities, enabling a better understanding of the cluster assembly history and mapping of the dark matter distribution. This initial dataset illustrates the diverse spectrum of legacy science that will be enabled by the Euclid survey

Euclid: Early Release Observations -- Dwarf galaxies in the Perseus galaxy cluster

International audienceWe make use of the unprecedented depth, spatial resolution, and field of view of the Euclid Early Release Observations of the Perseus galaxy cluster to detect and characterise the dwarf galaxy population in this massive system. The Euclid high resolution VIS and combined VIS+NIR colour images were visually inspected and dwarf galaxy candidates were identified. Their morphologies, the presence of nuclei, and their globular cluster (GC) richness were visually assessed, complementing an automatic detection of the GC candidates. Structural and photometric parameters, including Euclid filter colours, were extracted from 2-dimensional fitting. Based on this analysis, a total of 1100 dwarf candidates were found across the image, with 638 appearing to be new identifications. The majority (96%) are classified as dwarf ellipticals, 53% are nucleated, 26% are GC-rich, and 6% show disturbed morphologies. A relatively high fraction of galaxies, 8%, are categorised as ultra-diffuse galaxies. The majority of the dwarfs follow the expected scaling relations. Globally, the GC specific frequency, S_N, of the Perseus dwarfs is intermediate between those measured in the Virgo and Coma clusters. While the dwarfs with the largest GC counts are found throughout the Euclid field of view, those located around the east-west strip, where most of the brightest cluster members are found, exhibit larger S_N values, on average. The spatial distribution of the dwarfs, GCs, and intracluster light show a main iso-density/isophotal centre displaced to the west of the bright galaxy light distribution. The ERO imaging of the Perseus cluster demonstrates the unique capability of Euclid to concurrently detect and characterise large samples of dwarfs, their nuclei, and their GC systems, allowing us to construct a detailed picture of the formation and evolution of galaxies over a wide range of mass scales and environments

Euclid: Early Release Observations -- Overview of the Perseus cluster and analysis of its luminosity and stellar mass functions

International audienceThe Euclid ERO programme targeted the Perseus cluster of galaxies, gathering deep data in the central region of the cluster over 0.7 square degree, corresponding to approximately 0.25 r_200. The data set reaches a point-source depth of IE=28.0 (YE, JE, HE = 25.3) AB magnitudes at 5 sigma with a 0.16" and 0.48" FWHM, and a surface brightness limit of 30.1 (29.2) mag per square arcsec. The exceptional depth and spatial resolution of this wide-field multi-band data enable the simultaneous detection and characterisation of both bright and low surface brightness galaxies, along with their globular cluster systems, from the optical to the NIR. This study advances beyond previous analyses of the cluster and enables a range of scientific investigations summarised here. We derive the luminosity and stellar mass functions (LF and SMF) of the Perseus cluster in the Euclid IE band, thanks to supplementary u,g,r,i,z and Halpha data from the CFHT. We adopt a catalogue of 1100 dwarf galaxies, detailed in the corresponding ERO paper. We identify all other sources in the Euclid images and obtain accurate photometric measurements using AutoProf or AstroPhot for 138 bright cluster galaxies, and SourceExtractor for half a million compact sources. Cluster membership for the bright sample is determined by calculating photometric redshifts with Phosphoros. Our LF and SMF are the deepest recorded for the Perseus cluster, highlighting the groundbreaking capabilities of the Euclid telescope. Both the LF and SMF fit a Schechter plus Gaussian model. The LF features a dip at M(IE)=-19 and a faint-end slope of alpha_S = -1.2 to -1.3. The SMF displays a low-mass-end slope of alpha_S = -1.2 to -1.35. These observed slopes are flatter than those predicted for dark matter halos in cosmological simulations, offering significant insights for models of galaxy formation and evolution

Euclid: Early Release Observations -- Overview of the Perseus cluster and analysis of its luminosity and stellar mass functions

International audienceThe Euclid ERO programme targeted the Perseus cluster of galaxies, gathering deep data in the central region of the cluster over 0.7 square degree, corresponding to approximately 0.25 r_200. The data set reaches a point-source depth of IE=28.0 (YE, JE, HE = 25.3) AB magnitudes at 5 sigma with a 0.16" and 0.48" FWHM, and a surface brightness limit of 30.1 (29.2) mag per square arcsec. The exceptional depth and spatial resolution of this wide-field multi-band data enable the simultaneous detection and characterisation of both bright and low surface brightness galaxies, along with their globular cluster systems, from the optical to the NIR. This study advances beyond previous analyses of the cluster and enables a range of scientific investigations summarised here. We derive the luminosity and stellar mass functions (LF and SMF) of the Perseus cluster in the Euclid IE band, thanks to supplementary u,g,r,i,z and Halpha data from the CFHT. We adopt a catalogue of 1100 dwarf galaxies, detailed in the corresponding ERO paper. We identify all other sources in the Euclid images and obtain accurate photometric measurements using AutoProf or AstroPhot for 138 bright cluster galaxies, and SourceExtractor for half a million compact sources. Cluster membership for the bright sample is determined by calculating photometric redshifts with Phosphoros. Our LF and SMF are the deepest recorded for the Perseus cluster, highlighting the groundbreaking capabilities of the Euclid telescope. Both the LF and SMF fit a Schechter plus Gaussian model. The LF features a dip at M(IE)=-19 and a faint-end slope of alpha_S = -1.2 to -1.3. The SMF displays a low-mass-end slope of alpha_S = -1.2 to -1.35. These observed slopes are flatter than those predicted for dark matter halos in cosmological simulations, offering significant insights for models of galaxy formation and evolution

Euclid: Early Release Observations -- Dwarf galaxies in the Perseus galaxy cluster

International audienceWe make use of the unprecedented depth, spatial resolution, and field of view of the Euclid Early Release Observations of the Perseus galaxy cluster to detect and characterise the dwarf galaxy population in this massive system. The Euclid high resolution VIS and combined VIS+NIR colour images were visually inspected and dwarf galaxy candidates were identified. Their morphologies, the presence of nuclei, and their globular cluster (GC) richness were visually assessed, complementing an automatic detection of the GC candidates. Structural and photometric parameters, including Euclid filter colours, were extracted from 2-dimensional fitting. Based on this analysis, a total of 1100 dwarf candidates were found across the image, with 638 appearing to be new identifications. The majority (96%) are classified as dwarf ellipticals, 53% are nucleated, 26% are GC-rich, and 6% show disturbed morphologies. A relatively high fraction of galaxies, 8%, are categorised as ultra-diffuse galaxies. The majority of the dwarfs follow the expected scaling relations. Globally, the GC specific frequency, S_N, of the Perseus dwarfs is intermediate between those measured in the Virgo and Coma clusters. While the dwarfs with the largest GC counts are found throughout the Euclid field of view, those located around the east-west strip, where most of the brightest cluster members are found, exhibit larger S_N values, on average. The spatial distribution of the dwarfs, GCs, and intracluster light show a main iso-density/isophotal centre displaced to the west of the bright galaxy light distribution. The ERO imaging of the Perseus cluster demonstrates the unique capability of Euclid to concurrently detect and characterise large samples of dwarfs, their nuclei, and their GC systems, allowing us to construct a detailed picture of the formation and evolution of galaxies over a wide range of mass scales and environments

Euclid: Early Release Observations -- Overview of the Perseus cluster and analysis of its luminosity and stellar mass functions

International audienceThe Euclid ERO programme targeted the Perseus cluster of galaxies, gathering deep data in the central region of the cluster over 0.7 square degree, corresponding to approximately 0.25 r_200. The data set reaches a point-source depth of IE=28.0 (YE, JE, HE = 25.3) AB magnitudes at 5 sigma with a 0.16" and 0.48" FWHM, and a surface brightness limit of 30.1 (29.2) mag per square arcsec. The exceptional depth and spatial resolution of this wide-field multi-band data enable the simultaneous detection and characterisation of both bright and low surface brightness galaxies, along with their globular cluster systems, from the optical to the NIR. This study advances beyond previous analyses of the cluster and enables a range of scientific investigations summarised here. We derive the luminosity and stellar mass functions (LF and SMF) of the Perseus cluster in the Euclid IE band, thanks to supplementary u,g,r,i,z and Halpha data from the CFHT. We adopt a catalogue of 1100 dwarf galaxies, detailed in the corresponding ERO paper. We identify all other sources in the Euclid images and obtain accurate photometric measurements using AutoProf or AstroPhot for 138 bright cluster galaxies, and SourceExtractor for half a million compact sources. Cluster membership for the bright sample is determined by calculating photometric redshifts with Phosphoros. Our LF and SMF are the deepest recorded for the Perseus cluster, highlighting the groundbreaking capabilities of the Euclid telescope. Both the LF and SMF fit a Schechter plus Gaussian model. The LF features a dip at M(IE)=-19 and a faint-end slope of alpha_S = -1.2 to -1.3. The SMF displays a low-mass-end slope of alpha_S = -1.2 to -1.35. These observed slopes are flatter than those predicted for dark matter halos in cosmological simulations, offering significant insights for models of galaxy formation and evolution

Euclid: Early Release Observations -- Overview of the Perseus cluster and analysis of its luminosity and stellar mass functions

International audienceThe Euclid ERO programme targeted the Perseus cluster of galaxies, gathering deep data in the central region of the cluster over 0.7 square degree, corresponding to approximately 0.25 r_200. The data set reaches a point-source depth of IE=28.0 (YE, JE, HE = 25.3) AB magnitudes at 5 sigma with a 0.16" and 0.48" FWHM, and a surface brightness limit of 30.1 (29.2) mag per square arcsec. The exceptional depth and spatial resolution of this wide-field multi-band data enable the simultaneous detection and characterisation of both bright and low surface brightness galaxies, along with their globular cluster systems, from the optical to the NIR. This study advances beyond previous analyses of the cluster and enables a range of scientific investigations summarised here. We derive the luminosity and stellar mass functions (LF and SMF) of the Perseus cluster in the Euclid IE band, thanks to supplementary u,g,r,i,z and Halpha data from the CFHT. We adopt a catalogue of 1100 dwarf galaxies, detailed in the corresponding ERO paper. We identify all other sources in the Euclid images and obtain accurate photometric measurements using AutoProf or AstroPhot for 138 bright cluster galaxies, and SourceExtractor for half a million compact sources. Cluster membership for the bright sample is determined by calculating photometric redshifts with Phosphoros. Our LF and SMF are the deepest recorded for the Perseus cluster, highlighting the groundbreaking capabilities of the Euclid telescope. Both the LF and SMF fit a Schechter plus Gaussian model. The LF features a dip at M(IE)=-19 and a faint-end slope of alpha_S = -1.2 to -1.3. The SMF displays a low-mass-end slope of alpha_S = -1.2 to -1.35. These observed slopes are flatter than those predicted for dark matter halos in cosmological simulations, offering significant insights for models of galaxy formation and evolution