Anti-Contraction Effects of Euscaphic Acid Isolated from Crataegus azarolus var. aronia L on Rat’s Aortic Smooth Muscle

The current study represents the first attempt to investigate the effect of the Euscaphic acid (EA) on Rats isolated  thoracic aortic smooth muscle cells. Isolated aorta was used to test the anti-contraction effects and the possible mode of action(s) of the EA (1*10-7 M) and (3*10-7 M) isolated from Crataegus azarolus var. aronia L. Euscaphic acid showed high anti-contraction effects on norepinephrin (NE), (1*10-9-10-4 M) induced contraction in aortic smooth muscle cells in endothelium-intact, endothelium-denuded, and aortic rings pre-incubated with potassium (K+)-channels blocker (tetraethylammonium, TEA), prostaglandin I2 (PGI2) inhibitor (indomethacin) and cyclic guanosine monophosphate (cGMP) inhibitor ( methylene blue). On the other hand, other K+ channels subtype blockers glibenclamide (GLIB); barium chloride (BaCl2) and 4-aminopyridine (4-AP) demonstrated that adenosine triphosphate sensitive K+ (KATP), inwardly rectifying K+ (Kir) and voltage-dependent K+ (KV) channels played no role in anti-contraction induced by EA. Furthermore, the role of L-types calcium (Ca++) channels in EA anti-contractile effects on aortic smooth muscle cells was proved, by using the Ca++-channel blocker verapamil, as indicated by the production of a potent anti-contraction effect . The results of the current study indicate that the anti-contraction effects of EA may be due to the activation of calcium dependent, K+ (KCa) channels and blocking of L-type Ca++ channels. Thus, from these results it can be concluded that both K+ and Ca++ channels play an important role in anti-contraction effects of EA, which are mediated possibly through opening of KCa channels and blockade of voltage-dependent calcium channels, which may justify the use of medicinal plant C. azarolus in cardiovascular disease. Keywords: Crataegus azarolus var. aronia, Euscaphic acid, smooth muscle cells, K+-channels blockers, Ca++-channels blocker

Structure аnd Physical-Mechanical Properties of nc-TiN Coatings Obtained by Vacuum-Arc Deposition and Deposition with HF Discharge

Using the scanning electron microscopy (SEM), X-ray diffraction (XRD) analysis, adhesion strength, friction coefficient, and wear rate of the material we have studied properties of nc-TiN coatings. Depending on the bias potential applied to the substrate and the chamber pressure, the inclusion of HF discharge, it is shown that combination of different parameters recorded during scratching allows to distinguish the threshold values of the critical load which are linked to different types of cohesive and adhesive fracture of coatings in tribological tests. Sizes of nc-TiN nanograins, stoichiometry of coatings, as well as the phase and elemental composition and morphology of the coating surface were determined. When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/3599

Acanthamoeba genotype T4 detected in naturally-infected feline corneas found to be in homology with those causing human keratitis

A total of 10 out of 65 cornea swab samples from cats with eye symptoms showed Acanthamoeba-like morphology after cultivation. By PCR and DNA sequencing of Acanthamoeba diagnostic fragment 3 (DF3), all 10 isolates from the positive samples were categorized into two homologous groups of AfC1 (PM1, PM2, PM3, PF6, KM7, KF8, KMK9) and AfC2 (PM4, PM5, KFK10) due to the presence of bases A(354) and G(354), respectively. Furthermore, DF3 of AfC1 and AfC2 showed 100 similarity with Genbank reference isolates with the accession numbers DQ087314, EU146073 and U07401, GU808323, which were Acanthamoeba castellanii strains genotype T4 originating from human keratitis. This finding suggests that A. castellani strains have the capability to infect cats and human under favorable conditions

Impact of soy milk consumption on cardiometabolic risk factors: A systematic review and meta-analysis of randomized controlled trials

Background: Soy milk contains some beneficial components such as isoflavones which can exert favorable effects on the cardiovascular health. The current study aimed to comprehensively evaluate the potential effects of soy milk consumption on cardiometabolic risk factors in adults. Methods: Relevant articles published up to June 2020 were systematically retrieved from SCOPUS, PubMed/MEDLINE, EMBASE, and Web of Science databases. In our study, we included all the randomized controlled trials (RCTs) investigating the impact of soy milk consumption on various cardiometabolic risk factors in adults (age � 18 years). A meta-analysis of the eligible studies was performed using the random-effects model. Results: The quantitative meta-analysis of 18 eligible RCTs (665 participants, age range 18�65 years) demonstrated that the consumption of soy milk significantly reduced systolic (P < 0.001) and diastolic (P = 0.002) blood pressure, total (P = 0.001) and low-density lipoprotein (P = 0.041) cholesterol, waist circumference (P = 0.005), C-reactive protein (P < 0.001), and tumor necrosis factor-alpha (P = 0.016). Significant between-study heterogeneity was found for the pooled effect sizes of blood pressure and low-density lipoprotein cholesterol. In addition, the subgroup analyses indicated that the decrease in systolic blood pressure (SBP) was more pronounced when soy milk was consumed for � 4 weeks. However, there were no significant differences between soy milk and control groups for the other factors, namely body weight, body mass index (BMI), high-density lipoprotein cholesterol, triglycerides, fasting blood glucose (FBG), and fasting insulin, interleukin-6, and fibrinogen. Conclusions: The current systematic review and meta-analysis revealed that incorporating soy milk into the diet might favorably affect several cardiometabolic risk factors in both healthy and unhealthy individuals. © 2021 The Author

Narcolepsy and adjuvanted pandemic influenza A (H1N1) 2009 vaccines – Multi-country assessment

Background: In 2010, a safety signal was detected for narcolepsy following vaccination with Pandemrix, an AS03-adjuvanted monovalent pandemic H1N1 influenza (pH1N1) vaccine. To further assess a possible association and inform policy on future use of adjuvants, we conducted a multi-country study of narcolepsy and adjuvanted pH1N1 vaccines. Methods: We used electronic health databases to conduct a dynamic retrospective cohort study to assess narcolepsy incidence rates (IR) before and during pH1N1 virus circulation, and after pH1N1 vaccination campaigns in Canada, Denmark, Spain, Sweden, Taiwan, the Netherlands, and the United Kingdom. Using a case-control study design, we evaluated the risk of narcolepsy following AS03- and MF59-adjuvanted pH1N1 vaccines in Argentina, Canada, Spain, Switzerland, Taiwan, and the Netherlands. In the Netherlands, we also conducted a case-coverage study in children born between 2004 and 2009. Results: No changes in narcolepsy IRs were observed in any periods in single study sites except Sweden and Taiwan; in Taiwan incidence increased after wild-type pH1N1 virus circulation and in Sweden (a previously identified signaling country), incidence increased after the start of pH1N1 vaccination. No association was observed for Arepanrix-AS03 or Focetria-MF59 adjuvanted pH1N1 vaccines and narcolepsy in children or adults in the case-control study nor for children born between 2004 and 2009 in the Netherlands case-coverage study for Pandemrix-AS03. Conclusions: Other than elevated narcolepsy IRs in the period after vaccination campaigns in Sweden, we did not find an association between AS03- or MF59-adjuvanted pH1N1 vaccines and narcolepsy in children or adults in the sites studied, although power to evaluate the AS03-adjuvanted Pandemrix brand vaccine was limited in our study

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Studies on Streptococcus pneumoniae associated with pulmonary infection among HIV clients attending Aminu Kano Teaching Hospital (AKTH)

Respiratory infection is highly prevalent in HIV-infected patients and S. pneumoniae was a leading cause. The aim&nbsp;&nbsp; of this study was to isolate and identify Streptococcus pneumoniae associated with pulmonary infection among HIV Clients Attending Aminu Kano Teaching Hospital (AKTH). A total of 300 samples comprising&nbsp;&nbsp; sample from 180 HIV seropositive patients and 120 HIV seronegative patients as a (control) were enrolled in this study.) For the 180 HIV seropositive patients, 105(58.3%) were females, and 75(41.7%) were males. Among the HIV negative subjects 64(53.3%) were males and 56(46.7%) were females. The incident of Streptococcus pneumoniae isolates associated with pneumonia in HIV/AIDS seropositive patients&nbsp; in relation to age group in this&nbsp; study showed the higher&nbsp; prevalence of 15(51.72%),&nbsp; among the age groups of 30-39, had the highest&nbsp; of prevalence of 15(51.72%), which occur more&nbsp; common in HIV infected patients while for HIV seronegative patients age group 10-19&nbsp; had the highest&nbsp; incidence of&nbsp; 7(29.17%).Result showed no significant difference in the prevalence of&nbsp; S .pneumoniae among the HIV seropositive and seronegative patients (P &gt; 0.05) .Antimicrobial susceptibility showed that Ciprofloxacin 25(86.2%), was found to be most active invitro against the isolates followed by Oxacillin 21(72.4%), Augmentin19&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; (65.5), Chloramphenicol 15(51.7%). Tetracycline, 14(48.3%), Clindamycin 14(48.3) and Erythromycin 10(34.5%). Result showed no significant difference in the levels of antibiotic susceptibilities and resistance observed (p &gt; 0.05). The CD4 cells counts for the HIV seropositve patients were 161(89.4%) 350 cells/ml and 19(10.6%) &lt;350 cells/m recorded as normal and low respectively. There was significant difference (p &lt;0.05) for &nbsp;the CD4 cells counts of the study population with the prevalence of Streptococcus pneumoniae isolates among the HIV seropositive patients&nbsp; (P 0.000 &lt;0.05).&nbsp; PCR amplification&nbsp; for Streptococcus pneumoniae penicillin resistance genes showed that Only 2(33.3%)&nbsp; out of 6&nbsp; resistant&nbsp; isolates&nbsp; possed the pneumococcal pbp2b resistance gene. According to the findings, the pbp2b gene can play a role of fundamentalimportance in the resistance of S. pneumoniae.&nbsp; Modify heterogeneity in antibiotic use, which several antibiotics are taken in a rotation against taking just 1 antibiotic such that isolates resistant to 1 antibiotic are killed when the subsequent antibiotic is taken. Keywords: HIV/AIDS,&nbsp; Streptococcus Pneumoniae, Antibiotic Resistance, Minimal Inhibitory Concentration (MIC), Pbp2b&nbsp; CD4 cells count

444 Doa Rasulullah saw

xxvi + 282 hal.; 16 cm