7 research outputs found

    Characterization and pathogenicity of Cylindrocarpon-like asexual morphs associated with black foot disease in algerian grapevine nurseries, with the description of Pleiocarpon algeriense sp. nov

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    [EN] During a survey of black foot disease in Algerian grapevine nurseries, a collection of 79 Cylindrocarpon-like isolates were obtained. Based on morphology and DNA sequence data of histone H3 (his3), three species of Dactylonectria were identified including Dactylonectria torresensis (40 isolates), D. macrodidyma (24 isolates) and D. novozelandica (14 isolates). In addition, one isolate belonging to the genus Pleiocarpon was found and it is described here as a new species, Pleiocarpon algeriense, based on morphological features and DNA sequence data of the internal transcribed spacer region (ITS), translation elongation factor 1-alpha (tef1), beta-tubulin (tub2), large subunit nrDNA (LSU) and histone H3 (his3). This is the first time that these species are reported in Algeria. Pathogenicity tests, were conducted with representative isolates from each species. All of them were able to induce typical necrosis symptoms on grapevine cuttings. These results emphasize the urgent need to implement an integrated management strategy for black foot disease in Algerian grapevine nurseries in order to reduce the incidence of this disease on grapevine planting material and to prevent that it spreads to new grapevine production areas.Much of this work was supported by the laboratory of the Grupo de Investigacion en Hongos Fitopatogenos, Instituto Agroforestal Mediterraneo (IAM), Universitat Politecnica de Valencia (UPV), Spain. W. Aigoun-Mouhous thanks the University of Blida for funding the research stay in Valencia, Spain. G. Elena was supported by the Spanish post-doctoral grant Juan de la Cierva-Formacion. A. Cabral was supported by Portuguese national funds through FundacAo para a Ciencia e a Tecnologia grant SFRH/BPD/84508/2012 and FCT Unit funding UID/AGR/04129/2013. This work was also supported by EFRR "Multidisciplinary research to increase application potential of nanomaterials in agricultural practice" (No. CZ.02.1.01/0.0/0.0/16_025/0007314).Aigoun-Mouhous, W.; Elena-Jiménez, G.; Cabral, A.; León Santana, M.; Sabaou, N.; Armengol Fortí, J.; Chaouia, C.... (2019). Characterization and pathogenicity of Cylindrocarpon-like asexual morphs associated with black foot disease in algerian grapevine nurseries, with the description of Pleiocarpon algeriense sp. nov. European Journal of Plant Pathology. 154(4):887-901. https://doi.org/10.1007/s10658-019-01708-zS8879011544Abreo, E., Martinez, S., Bettucci, L., & Lupo, S. (2010). Morphological and molecular characterisation of Campylocarpon and Cylindrocarpon spp. associated with black foot disease of grapevines in Uruguay. Australasian Plant Pathology, 39(5), 446–452.Agustí-Brisach, C., & Armengol, J. (2013). Black-foot disease of grapevine: an update on taxonomy, epidemiology and management strategies. 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    Cadophora sabaouae sp. nov. and Phaeoacremonium Species Associated with Petri Disease on Grapevine Propagation Material and Young Grapevines in Algeria

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    [EN] A field survey conducted on asymptomatic grapevine propagation material from nurseries and symptomatic young grapevines throughout different regions of Algeria yielded a collection of 70 Phaeoacremonium-like isolates and three Cadophora-like isolates. Based on morphology and DNA sequence data of I3-tubulin (tub2) and actin, five Phaeoacremonium species were identified including Phaeoacremonium minimum (22 isolates), Phaeoacremonium venezuelense (19 isolates), Phaeoacremonium parasiticum (17 isolates), Phaeoacremonium australiense (8 isolates), and Phaeoacremon bun ira nianu m (4 isolates). The latter two species (P. australiense and P. iranianum) were reported for the first time in Algeria. Multilocus phylogenetic analyses (internal transcribed spacer, tub2, and translation elongation factor 1-alpha) and morphological features, allowed the description of the three isolates belonging to the genus Cadophora (WAMC34, WAMC117, and WAMC118) as a novel species, named Cadophora sabaouae sp. nov. Pathogenicity tests were conducted on grapevine cuttings cultivar Cardinal. All the identified species were pathogenic on grapevine cuttings.This work was supported by the Ministerstvo Skolstvi, Mladeze a Telovychovy, Czech Republic under grant no. CZ-02-1-01/0-0/0-0/16-025/0007314, the Technologicka Agentura Ceske Republiky under grant no. TJ02000096, and the Spanish Government, Ramon y Cajal program under grant no. RYC-2017-23098 (to D. Gramaje).Aigoun-Mouhous, W.; Mahamedi, AE.; LeĂłn Santana, M.; Chaouia, C.; Zitouni, A.; Barankova, K.; Eichmeier, A.... (2021). Cadophora sabaouae sp. nov. and Phaeoacremonium Species Associated with Petri Disease on Grapevine Propagation Material and Young Grapevines in Algeria. Plant Disease. 105(11):3657-3668. https://doi.org/10.1094/PDIS-11-20-2380-RES365736681051

    Paecilomyces clematidis (Eurotiales, Thermoascaceae): a new species from Clematis root

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    During a survey of endophytic fungi associated with ornamental plants in the Czech Republic, Paecilomyces-like strains were isolated from the root of Clematis. Analyses based on a combined internal transcribed spacer region (ITS), beta-tubulin (tub2) and calmodulin (CaM) sequence data matrix were applied to infer the phylogenetic position of these isolates. The novel species is characterized by phialides with a cylindrical basal portion tapering to a thin long neck producing pyriform conidia in chains. The new species is introduced with comprehensive descriptions, illustrations and a phylogenetic tree herein. Two primer pairs targeting the partial CaM gene, cm1F/cm1R and cm2F/cm2R, were designed in this study.This work was supported by project No. IGA-ZF/2021-ST2003 and CZ.02.1.01/0.0/0.0/16_025/0007314

    Lasiodiplodia mitidjana sp. nov. and other Botryosphaeriaceae species causing branch canker and dieback of Citrus sinensis in Algeria.

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    Several Botryosphaeriaceae species are known to occur worldwide, causing dieback, canker and fruit rot on various hosts. Surveys conducted in ten commercial citrus orchards in the northern region of Algeria revealed five species of Botryosphaeriaceae belonging to three genera associated with diseased trees. Morphological and cultural characteristics as well as phylogenetic analyses of the internal transcribed spacer (ITS) region and the translation elongation factor 1-alpha (tef1-α) identified Diplodia mutila, Diplodia seriata, Dothiorella viticola, Lasiodiplodia mediterranea and a novel species which is here described as Lasiodiplodia mithidjana sp. nov.. Of these, L. mithidjana (14.1% of the samples) and L. mediterranea (13% of the samples) were the most widespread and abundant species. Pathogenicity tests revealed that L. mediterranea and D. seriata were the most aggressive species on citrus shoots. This study highlights the importance of Botryosphaeriaceae species as agents of canker and dieback of citrus trees in Algeria

    Defensive Mutualism of Endophytic Fungi: Effects of Sphaeropsidin A against a Model Lepidopteran Pest

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    Sphaeropsidin A (SphA) is a pimarane diterpene produced by several fungi associated with plants. Following previous evidence of insecticidal properties of SphA, we investigated its contact and oral toxicity against the model chewing lepidopteran Spodoptera littoralis. The compound showed no lethal effect when directly sprayed on larvae, while it produced an evident oral toxic effect, associated with sublethal effects. These results demonstrated that SphA might play a defensive role against lepidopteran insects in plants harboring the producing fungus, depending on the extent at which the endophytic strains are able to perform biosynthesis of this and eventually other bioactive metabolites in vivo

    Defensive Mutualism of Endophytic Fungi: Effects of Sphaeropsidin A against a Model Lepidopteran Pest

    No full text
    Sphaeropsidin A (SphA) is a pimarane diterpene produced by several fungi associated with plants. Following previous evidence of insecticidal properties of SphA, we investigated its contact and oral toxicity against the model chewing lepidopteran Spodoptera littoralis. The compound showed no lethal effect when directly sprayed on larvae, while it produced an evident oral toxic effect, associated with sublethal effects. These results demonstrated that SphA might play a defensive role against lepidopteran insects in plants harboring the producing fungus, depending on the extent at which the endophytic strains are able to perform biosynthesis of this and eventually other bioactive metabolites in vivo
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