Composition of Human Skin Microbiota Affects Attractiveness to Malaria Mosquitoes

The African malaria mosquito Anopheles gambiae sensu stricto continues to play an important role in malaria transmission, which is aggravated by its high degree of anthropophily, making it among the foremost vectors of this disease. In the current study we set out to unravel the strong association between this mosquito species and human beings, as it is determined by odorant cues derived from the human skin. Microbial communities on the skin play key roles in the production of human body odour. We demonstrate that the composition of the skin microbiota affects the degree of attractiveness of human beings to this mosquito species. Bacterial plate counts and 16S rRNA sequencing revealed that individuals that are highly attractive to An. gambiae s.s. have a significantly higher abundance, but lower diversity of bacteria on their skin than individuals that are poorly attractive. Bacterial genera that are correlated with the relative degree of attractiveness to mosquitoes were identified. The discovery of the connection between skin microbial populations and attractiveness to mosquitoes may lead to the development of new mosquito attractants and personalized methods for protection against vectors of malaria and other infectious diseases

A Push-Pull System to Reduce House Entry of Malaria Mosquitoes.

Mosquitoes are the dominant vectors of pathogens that cause infectious diseases such as malaria, dengue, yellow fever and filariasis. Current vector control strategies often rely on the use of pyrethroids against which mosquitoes are increasingly developing resistance. Here, a push-pull system is presented, that operates by the simultaneous use of repellent and attractive volatile odorants. Experiments were carried out in a semi-field set-up: a traditional house which was constructed inside a screenhouse. The release of different repellent compounds, para-menthane-3,8-diol (PMD), catnip oil e.o. and delta-undecalactone, from the four corners of the house resulted in significant reductions of 45% to 81.5% in house entry of host-seeking malaria mosquitoes. The highest reductions in house entry (up to 95.5%), were achieved by simultaneously repelling mosquitoes from the house (push) and removing them from the experimental set-up using attractant-baited traps (pull). The outcome of this study suggests that a push-pull system based on attractive and repellent volatiles may successfully be employed to target mosquito vectors of human disease. Reductions in house entry of malaria vectors, of the magnitude that was achieved in these experiments, would likely affect malaria transmission. The repellents used are non-toxic and can be used safely in a human environment. Delta-undecalactone is a novel repellent that showed higher effectiveness than the established repellent PMD. These results encourage further development of the system for practical implementation in the field

From Digital Divide to Information Availability: A Wi-Fi-Based Novel Solution for Information Dissemination

Digital divide means unequal access to the people for information and communication technology (ICT) facilities. The developed countries are comparatively less digitally divided as compared to developing countries. This study focuses on District Chitral considering its geographical conditions and high mountainous topography which plays a significant role in its isolation. Aside from the digital divide, the situation in Chitral is even more severe in terms of the absence of basic ICT infrastructure and electricity in the schools. To address this issue, especially in female secondary and higher secondary schools, we designed a project to bridge the digital divide via Wireless Local Area Network on Raspberry Pi3 for balancing the ICT facilities in the targeted area. The Wi-Fi-Based Content Distributors (Wi-Fi-BCDs) were provided to bridge the digital divide in rural area schools of Chitral. The Wi-Fi-BCD is a solar-based system that is used to deliver quality educational contents directly to classroom, library, or other learning environments without electricity connection and Internet wire as these facilities are available by default in it. The close-ended questionnaire was adopted to collect data from the students, teachers, and headmistresses of girl secondary and higher secondary schools in Chitral. The procedure of validity, reliability, regression, correlation, and exploratory factor analysis was used to analyze the obtained data. The technology acceptance model (TAM) was modified and adopted to examine the effects of Wi-Fi-BCD for bridging the digital divide. The relationship of the modified TAM model was examined through regression and correlation to verify the model fitness according to the data obtained. The result analysis of this study shows that the relationship of the modified TAM model with its variables is positively significant, while the analysis of path relationship between model variables and outcomes from the questionnaire shows that it motivates learners to use Wi-Fi-BCD

Targeting nucleolin for better survival in diffuse large B-cell lymphoma

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