1,280 research outputs found
Applications of Information Theory to Analysis of Neural Data
Information theory is a practical and theoretical framework developed for the
study of communication over noisy channels. Its probabilistic basis and
capacity to relate statistical structure to function make it ideally suited for
studying information flow in the nervous system. It has a number of useful
properties: it is a general measure sensitive to any relationship, not only
linear effects; it has meaningful units which in many cases allow direct
comparison between different experiments; and it can be used to study how much
information can be gained by observing neural responses in single trials,
rather than in averages over multiple trials. A variety of information
theoretic quantities are commonly used in neuroscience - (see entry
"Definitions of Information-Theoretic Quantities"). In this entry we review
some applications of information theory in neuroscience to study encoding of
information in both single neurons and neuronal populations.Comment: 8 pages, 2 figure
A tunable nanoplatform of nanogold functionalised with Angiogenin peptides for anti-angiogenic therapy of brain tumours
Angiogenin (ANG), an endogenous protein that plays a key role in cell growth and survival, has been scrutinised here as promising nanomedicine tool for the modulation of pro-/anti-angiogenic processes in brain cancer therapy. Specifically, peptide fragments from the putative cell membrane binding domain (residues 60-68) of the protein were used in this study to obtain peptide-functionalised spherical gold nanoparticles (AuNPs) of about 10 nm and 30 nm in optical and hydrodynamic size, respectively. Different hybrid biointerfaces were fabricated by peptide physical adsorption (Ang60-68) or chemisorption (the cysteine analogous Ang60-68Cys) at the metal nanoparticle surface, and cellular assays were performed in the comparison with ANG-functionalised AuNPs. Cellular treatments were performed both in basal and in copper-supplemented cell culture medium, to scrutinise the synergic effect of the metal, which is another known angiogenic factor. Two brain cell lines were investigated in parallel, namely tumour glioblastoma (A172) and neuron-like differentiated neuroblastoma (d-SH-SY5Y). Results on cell viability/proliferation, cytoskeleton actin, angiogenin translocation and vascular endothelial growth factor (VEGF) release pointed to the promising potentialities of the developed systems as anti-angiogenic tunable nanoplaftforms in cancer cells treatment
Ultraviolet and visible photometry of asteroid (21) Lutetia using the Hubble Space Telescope
The asteroid (21) Lutetia is the target of a planned close encounter by the
Rosetta spacecraft in July 2010. To prepare for that flyby, Lutetia has been
extensively observed by a variety of astronomical facilities. We used the
Hubble Space Telescope (HST) to determine the albedo of Lutetia over a wide
wavelength range, extending from ~150 nm to ~700 nm. Using data from a variety
of HST filters and a ground-based visible light spectrum, we employed synthetic
photometry techniques to derive absolute fluxes for Lutetia. New results from
ground-based measurements of Lutetia's size and shape were used to convert the
absolute fluxes into albedos. We present our best model for the spectral energy
distribution of Lutetia over the wavelength range 120-800 nm. There appears to
be a steep drop in the albedo (by a factor of ~2) for wavelengths shorter than
~300 nm. Nevertheless, the far ultraviolet albedo of Lutetia (~10%) is
considerably larger than that of typical C-chondrite material (~4%). The
geometric albedo at 550 nm is 16.5 +/- 1%. Lutetia's reflectivity is not
consistent with a metal-dominated surface at infrared or radar wavelengths, and
its albedo at all wavelengths (UV-visibile-IR-radar) is larger than observed
for typical primitive, chondritic material. We derive a relatively high FUV
albedo of ~10%, a result that will be tested by observations with the Alice
spectrograph during the Rosetta flyby of Lutetia in July 2010.Comment: 14 pages, 2 tables, 8 figure
Anti-angiogenic and anti-proliferative graphene oxide nanosheets for tumor cell therapy
Graphene oxide (GO) is a bidimensional novel material that exhibits high biocompatibility and angiogenic properties, mostly related to the intracellular formation of reactive oxygen species (ROS). In this work, we set up an experimental methodology for the fabrication of GO@peptide hybrids by the immobilization, via irreversible physical adsorption, of the Ac-(GHHPH)4-NH2 peptide sequence, known to mimic the anti-angiogenic domain of the histidine-proline-rich glycoprotein (HPRG). The anti-proliferative capability of the graphene-peptide hybrids were tested in vitro by viability assays on prostate cancer cells (PC-3 line), human neuroblastoma (SH-SY5Y), and human retinal endothelial cells (primary HREC). The anti-angiogenic response of the two cellular models of angiogenesis, namely endothelial and prostate cancer cells, was scrutinized by prostaglandin E2 (PGE2) release and wound scratch assays, to correlate the activation of inflammatory response upon the cell treatments with the GO@peptide nanocomposites to the cell migration processes. Results showed that the GO@peptide nanoassemblies not only effectively induced toxicity in the prostate cancer cells, but also strongly blocked the cell migration and inhibited the prostaglandin-mediated inflammatory process both in PC-3 and in HRECs. Moreover, the cytotoxic mechanism and the internalization efficiency of the theranostic nanoplatforms, investigated by mitochondrial ROS production analyses and confocal microscopy imaging, unraveled a dose-dependent manifold mechanism of action performed by the hybrid nanoassemblies against the PC-3 cells, with the detection of the GO-characteristic cell wrapping and mitochondrial perturbation. The obtained results pointed out to the very promising potential of the synthetized graphene-based hybrids for cancer therapy
The triaxial ellipsoid dimensions, rotational pole, and bulk density of ESA Rosetta target asteroid (21) Lutetia
We seek the best size estimates of the asteroid (21) Lutetia, the direction
of its spin axis, and its bulk density, assuming its shape is well described by
a smooth featureless triaxial ellipsoid, and to evaluate the deviations from
this assumption. Methods. We derive these quantities from the outlines of the
asteroid in 307 images of its resolved apparent disk obtained with adaptive
optics (AO) at Keck II and VLT, and combine these with recent mass
determinations to estimate a bulk density. Our best triaxial ellipsoid
diameters for Lutetia, based on our AO images alone, are a x b x c = 132 x 101
x 93 km, with uncertainties of 4 x 3 x 13 km including estimated systematics,
with a rotational pole within 5 deg. of ECJ2000 [long,lat] = [45, -7], or
EQJ2000 [RA, DEC] = [44, +9]. The AO model fit itself has internal precisions
of 1 x 1 x 8 km, but it is evident, both from this model derived from limited
viewing aspects and the radius vector model given in a companion paper, that
Lutetia has significant departures from an idealized ellipsoid. In particular,
the long axis may be overestimated from the AO images alone by about 10 km.
Therefore, we combine the best aspects of the radius vector and ellipsoid model
into a hybrid ellipsoid model, as our final result, of 124 +/- 5 x 101 +/- 4 x
93 +/- 13 km that can be used to estimate volumes, sizes, and projected areas.
The adopted pole position is within 5 deg. of [long, lat] = [52, -6] or[RA DEC]
= [52, +12]. Using two separately determined masses and the volume of our
hybrid model, we estimate a density of 3.5 +/- 1.1 or 4.3 +/- 0.8 g cm-3 . From
the density evidence alone, we argue that this favors an enstatite-chondrite
composition, although other compositions are formally allowed at the extremes
(low-porosity CV/CO carbonaceous chondrite or high-porosity metallic). We
discuss this in the context of other evidence.Comment: 9 pages, 8 figures, 5 tables, submitted to Astronomy and Astrophysic
Hamiltonian structure of real Monge-Amp\`ere equations
The real homogeneous Monge-Amp\`{e}re equation in one space and one time
dimensions admits infinitely many Hamiltonian operators and is completely
integrable by Magri's theorem. This remarkable property holds in arbitrary
number of dimensions as well, so that among all integrable nonlinear evolution
equations the real homogeneous Monge-Amp\`{e}re equation is distinguished as
one that retains its character as an integrable system in multi-dimensions.
This property can be traced back to the appearance of arbitrary functions in
the Lagrangian formulation of the real homogeneous Monge-Amp\`ere equation
which is degenerate and requires use of Dirac's theory of constraints for its
Hamiltonian formulation. As in the case of most completely integrable systems
the constraints are second class and Dirac brackets directly yield the
Hamiltonian operators. The simplest Hamiltonian operator results in the
Kac-Moody algebra of vector fields and functions on the unit circle.Comment: published in J. Phys. A 29 (1996) 325
Dark-bright magneto-exciton mixing induced by Coulomb interaction in strained quantum wells
Coupled magneto-exciton states between allowed (`bright') and forbidden
(`dark') transitions are found in absorption spectra of strained
InGaAs/GaAs quantum wells with increasing magnetic field up to
30 T. We found large (~ 10 meV) energy splittings in the mixed states. The
observed anticrossing behavior is independent of polarization, and sensitive
only to the parity of the quantum confined states. Detailed experimental and
theoretical investigations indicate that the excitonic Coulomb interaction
rather than valence band complexity is responsible for the splittings. In
addition, we determine the spin composition of the mixed states.Comment: 4 pages, 4 figure
Differential Calculi on Associative Algebras and Integrable Systems
After an introduction to some aspects of bidifferential calculus on
associative algebras, we focus on the notion of a "symmetry" of a generalized
zero curvature equation and derive Backlund and (forward, backward and binary)
Darboux transformations from it. We also recall a matrix version of the binary
Darboux transformation and, inspired by the so-called Cauchy matrix approach,
present an infinite system of equations solved by it. Finally, we sketch recent
work on a deformation of the matrix binary Darboux transformation in
bidifferential calculus, leading to a treatment of integrable equations with
sources.Comment: 19 pages, to appear in "Algebraic Structures and Applications", S.
Silvestrov et al (eds.), Springer Proceedings in Mathematics & Statistics,
202
Concurrent AFG3L2 and SPG7 mutations associated with syndromic parkinsonism and optic atrophy with aberrant OPA1 processing and mitochondrial network fragmentation
Mitochondrial dynamics and quality control are crucial for neuronal survival and their perturbation is a major cause of neurodegeneration. m-AAA complex is an ATP-dependent metalloprotease located in the inner mitochondrial membrane and involved in protein quality control. Mutations in the m-AAA subunits AFG3L2 and paraplegin are associated with autosomal dominant spinocerebellar ataxia (SCA28) and autosomal recessive hereditary spastic paraplegia (SPG7), respectively. We report a novel m-AAA-associated phenotype characterized by early-onset optic atrophy with spastic ataxia and L-Dopa-responsive parkinsonism. The proband carried a de-novo AFG3L2 heterozygous mutation (p.R468C) along with a heterozygous maternally-inherited intragenic deletion of SPG7. Functional analysis in yeast demonstrated the pathogenic role of AFG3L2 p.R468C mutation shedding light on its pathogenic mechanism. Analysis of patient's fibroblasts showed an abnormal processing pattern of OPA1, a dynamin-related protein essential for mitochondrial fusion and responsible for most cases of hereditary optic atrophy. Consistently, assessment of mitochondrial morphology revealed a severe fragmentation of the mitochondrial network, not observed in SCA28 and SPG7 patients\u2019 cells. This case suggests that coincidental mutations in both components of the mitochondrial m-AAA protease may result in a complex phenotype and reveals a crucial role for OPA1 processing in the pathogenesis of neurodegenerative disease caused by m-AAA defects
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