575 research outputs found

    Faktor-faktor yang Memengaruhi Angka Kebuntingan (Conception Rate) pada Sapi Potong Setelah Dilakukan Sinkronisasi Estrus di Kabupaten Lampung Tengah

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    This research used 287 cattle cows owned by 198 breeders, involve 3 sub-districts in Central Lampung District.This research aimed to determine factors affecting conception rate after oestrous synchronization in cattle cows inCentral Lampung District, Lampung Province in November 2012-Februari 2013.The result of this research showed that the conception rate cattle cows after oestrous synchronization in CentralLampung District was 60,53 %, included in less good category. The factors affecting conception rate after oestroussynchronization in Central Lampung District were the amount of pasture given which positively associate with thefactor amount of 0,020, the shape of cage wall which negatively associate with the factor amount of 1,473, thematerial of cage floor which positively associate with the factor amount of 0,701, the cage width which negativelyassociate with the factor amount of 0,062, & the breeding long time which positively associate with the factoramount of 0,057

    Evaluation of two lyophilized molecular assays to rapidly detect foot-and-mouth disease virus directly from clinical samples in field settings

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    Accurate, timely diagnosis is essential for the control, monitoring and eradication of foot‐and‐mouth disease (FMD). Clinical samples from suspect cases are normally tested at reference laboratories. However, transport of samples to these centralized facilities can be a lengthy process that can impose delays on critical decision making. These concerns have motivated work to evaluate simple‐to‐use technologies, including molecular‐based diagnostic platforms, that can be deployed closer to suspect cases of FMD. In this context, FMD virus (FMDV)‐specific reverse transcription loop‐mediated isothermal amplification (RT‐LAMP) and real‐time RT‐PCR (rRT‐PCR) assays, compatible with simple sample preparation methods and in situ visualization, have been developed which share equivalent analytical sensitivity with laboratory‐based rRT‐PCR. However, the lack of robust ‘ready‐to‐use kits’ that utilize stabilized reagents limits the deployment of these tests into field settings. To address this gap, this study describes the performance of lyophilized rRT‐PCR and RT‐LAMP assays to detect FMDV. Both of these assays are compatible with the use of fluorescence to monitor amplification in real‐time, and for the RT‐LAMP assays end point detection could also be achieved using molecular lateral flow devices. Lyophilization of reagents did not adversely affect the performance of the assays. Importantly, when these assays were deployed into challenging laboratory and field settings within East Africa they proved to be reliable in their ability to detect FMDV in a range of clinical samples from acutely infected as well as convalescent cattle. These data support the use of highly sensitive molecular assays into field settings for simple and rapid detection of FMDV

    Exact results on spin dynamics and multiple quantum dynamics in alternating spin-1/2 chains with XY-Hamiltonian at high temperatures

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    We extend the picture of a transfer of nuclear spin-1/2 polarization along a homogeneous one-dimensional chain with the XY-Hamiltonian to the inhomogeneous chain with alternating nearest neighbour couplings and alternating Larmor frequencies. To this end, we calculate exactly the spectrum of the spin-1/2 XY-Hamiltonian of the alternating chain with an odd number of sites. The exact spectrum of the XY-Hamiltonian is also applied to study the multiple quantum (MQ) NMR dynamics of the alternating spin-1/2 chain. MQ NMR spectra are shown to have the MQ coherences of zero and ±\pm second orders just as in the case of a homogeneous chain. The intensities of the MQ coherences are calculated.Comment: 10 pages, 4 figure

    Investigation of continuous-time quantum walk by using Krylov subspace-Lanczos algorithm

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    In papers\cite{js,jsa}, the amplitudes of continuous-time quantum walk on graphs possessing quantum decomposition (QD graphs) have been calculated by a new method based on spectral distribution associated to their adjacency matrix. Here in this paper, it is shown that the continuous-time quantum walk on any arbitrary graph can be investigated by spectral distribution method, simply by using Krylov subspace-Lanczos algorithm to generate orthonormal bases of Hilbert space of quantum walk isomorphic to orthogonal polynomials. Also new type of graphs possessing generalized quantum decomposition have been introduced, where this is achieved simply by relaxing some of the constrains imposed on QD graphs and it is shown that both in QD and GQD graphs, the unit vectors of strata are identical with the orthonormal basis produced by Lanczos algorithm. Moreover, it is shown that probability amplitude of observing walk at a given vertex is proportional to its coefficient in the corresponding unit vector of its stratum, and it can be written in terms of the amplitude of its stratum. Finally the capability of Lanczos-based algorithm for evaluation of walk on arbitrary graphs (GQD or non-QD types), has been tested by calculating the probability amplitudes of quantum walk on some interesting finite (infinite) graph of GQD type and finite (infinite) path graph of non-GQD type, where the asymptotic behavior of the probability amplitudes at infinite limit of number of vertices, are in agreement with those of central limit theorem of Ref.\cite{nko}.Comment: 29 pages, 4 figure

    Um modelo integrado de gestĂŁo do risco agrĂ­cola para o Brasil.

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    O seguro agrĂ­cola Ă© um dos mais importantes instrumentos para o desenvolvimento do setor agrĂ­cola, pois, ao permitir proteção ao produtor rural contra efeitos adversos de eventos ambientais e do mercado torna-se indispensĂĄvel Ă  estabilidade da renda, Ă  geração de emprego e ao desenvolvimento tecnolĂłgico. No setor agrĂ­cola, alĂ©m do risco de mercado, existem diversas outras fontes que a tornam uma atividade eminentemente arriscada. A principal delas se refere ao fato de que a atividade agrĂ­cola Ă© altamente dependente de condiçÔes ambientais de difĂ­cil controle pelo homem, de modo que as variĂĄveis climĂĄticas e sua interação com fatores biĂłticos podem influenciar sobremaneira o resultado final da safra. A realidade histĂłrica mostra que o seguro agrĂ­cola permite ao produtor rural manter sua renda na ocorrĂȘncia de um sinistro, o que Ă© fundamental para o setor agrĂ­cola e a sociedade em geral

    Tracking global changes induced in the CD4 T-cell receptor repertoire by immunization with a complex antigen using short stretches of CDR3 protein sequence.

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    The clonal theory of adaptive immunity proposes that immunological responses are encoded by increases in the frequency of lymphocytes carrying antigen-specific receptors. In this study, we measure the frequency of different T-cell receptors (TcR) in CD4 + T cell populations of mice immunized with a complex antigen, killed Mycobacterium tuberculosis, using high throughput parallel sequencing of the TcRÎČ chain. Our initial hypothesis that immunization would induce repertoire convergence proved to be incorrect, and therefore an alternative approach was developed that allows accurate stratification of TcR repertoires and provides novel insights into the nature of CD4 + T-cell receptor recognition
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