Microbiological surveillance of operation theatres, intensive care units and labor room of a teaching hospital in Telangana, India

Background: Hospital-associated infections are the major cause of patient morbidity and mortality. Environmental monitoring by the microbiological testing of surfaces and equipments is useful to detect changing trends of types and counts of microbial flora. The aims of the study were to count CFU (colony forming unit) rate of indoor air, to identify bacterial colonization of surface and equipments isolated from Operation theatres, ICUs and Labor room of a teaching hospital in Telangana, India.Methods: Air sampling of 10 OT’s, 4 ICU’s and 1 LR were done by settle plate method. Swabs were taken from different sites and equipments and bacterial species were isolated and identified from them.Results: Gynaecology OT-2 and 3 showed less bacterial CFU rate of air (6 CFU/m3) followed by Opthamology OT (16 CFU/m3) and highest in Orthopaedics minor OT (72 CFU/m3) and Dental OT (42 CFU/m3 with 1 fungal CFU/m3). Pediatric ICU showed less bacterial CFU rate (28 CFU/m3) followed by Surgery ICU (40 CFU/m3) and highest in Medical ICU (100 CFU/m3 and 5 fungal CFU/m3). Labor room showed 65 CFU/m3. Bacterial species were isolated from 48 (43%) out of total 111 swab samples taken from all OTs and ICUs. The most common isolate was Bacillus species 36 (75%) followed by micrococcus (54%). Pathogenic organisms isolated were 12 Gram negative bacilli and 6 gram positive cocci, the common isolate was Klebsiella spp (7 of 12).Conclusions: The study shows that OTs were having bacterial CFU rate of air varying from 6-72 CFU/m3 and colonized by contaminants like Bacillus sp and pathogens like Klebsiella sp. ICUs were having bacterial CFU rate of air varying from 28-100 CFU/m3 and colonized with contaminant like Bacillus sp., as well as potential pathogens like Klebsiella, Pseudomonas etc. Fungal CFU were also seen both in OTs and ICUs. High level of microbial contamination indicates the needs for periodic surveillance aimed at early detection of bacterial contamination levels and prevention of hospital acquired infections

Influence of rearrangement of actin cytoskeleton on the overall material properties of ATDC5 cells during chondrogenesis

Studies show that when chondrocytes are cultured in 2D, they lose their phenotype. This change is reversed when the cells are embedded in a 3D culture system such as in agarose gel. Previous experiments in our lab showed that when prechodrocytic cells were embedded in agarose, chondrogenesis was triggered in these cells. This work attempts to understand the mechanical implications of this behavior. It also provides an estimate of the sensitivity of the apparatus that would be required to carry out compression loading experiments of gel-cell constructs that can distinguish between pre-chondrocytes and chondrocytes.;ATDC5 cells were cultured in 3D for 12 days and fluorescent images of the actin cytoskeletal structure of the cells were obtained. A major reorientation of the cytoskeletal structure (from stress-fiber to punctate appearance) was observed. This data was then used to construct a Finite Element model of cell. The cell was considered as a composite matrix and the actin as the embedded fibers. Radial deformations of the cell (difference in the corresponding undeformed and deformed radii of the cell at a particular angle w.r.t. loading axis) were calculated for 5 sets of experimental data. (Abstract shortened by UMI.)

Optimal Remote Qubit Teleportation Using Node2vec

Much research work is done on implementing quantum teleportation and entanglement swapping for remote entanglement. Due to dynamical topological changes in quantum networks, nodes have to construct the shortest paths every time they want to communicate with a remote neighbour. But due to the entanglement failures remote entanglement establishment is still a challenging task. Also as the nodes know only about their neighbouring nodes computing optimal paths between source and remote nodes is time consuming too. In finding the next best neighbour in the optimal path between a given source and remote nodes so as to decrease the entanglement cost, deep learning techniques can be applied. In this paper we defined throughput of the quantum network as the maximum qubits transmitted with minimum entanglement cost. Much of research work is done to improve the throughput of the quantum network using the deep learning techniques. In this paper we adopted deep learning techniques for implementing remote entanglement between two non-neighbour nodes using remote qubit teleportation and entanglement swapping. The proposed method called Optimal Remote Qubit Teleportation outperforms the throughput obtained by the state of art approach

SIMULTANEOUS REVERSE-PHASE ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF GRAZOPREVIR AND ELBASVIR

 Objective: The objective of this study was to develop and validate rapid, specific, sensitive, and precise reverse-phase ultra performance liquid chromatography (RP-UPLC) method for the quantitative determination of grazoprevir and elbasvir, as there are no official monograph and no analytical method by UPLC.Methods: Chromatographic separation was achieved on a Waters Acquity UPLC HSS C18 (2.1 mm × 100 mm, 1.8 micron) column with a 45:55 (v/v) mixture of 0.1% orthophosphoric acid (pH 2.8) and acetonitrile as a mobile phase, thermostated at 30°C with a short run time of 3.0 min.Results: The retention times were 0.73 and 1.29 min for grazoprevir and elbasvir, respectively. Quantification is achieved with TUV detection at 254 nm over the concentration range of 25–150 μg/ml for grazoprevir and for elbasvir 12.5–75 μg/ml, with a correlation coefficient of 0.999 and 0.999, respectively. The developed method was validated according to the International Conference on Harmonization (ICH) guidelines with respect to linearity, accuracy, precision, specificity, and robustness. Forced degradation study was extended out under acidic, alkaline, oxidative, photolytic, and thermal conditions to demonstrate the stability-indicating capability of the developed UPLC method. The degradation products were well resolved from the main peak, thus proved the stability-indicating power of the method. The results of the analysis were validated statistically.Conclusion: The method is precise, accurate, linear, robust, and fast. The short retention time allows the analysis of a large number of samples in a short period of time and, therefore, should be cost-effective for routine analysis in the pharmaceutical industry

Impact of honey-enriched mulberry diet on the energy metabolism of the silkworm, Bombyx mori

The present study was taken-up with a view to clearly define the role of oxidative phosphorylation vis-a-vis transdeamination in Bombyx mori metamorphosis, under the influence of honey-enriched mulberry diet. Therefore, the study examined the accumulation and utilization patterns of carbohydrate (glycogen, trehalose, glucose) and non-carbohydrate energy reserves (proteins, amino acids) in its fat body during larval, pupal and adult stages. In accordance with Hutchinson’s investment principle, the energy reserves invested during larval stage are partly used in pupal stage and those invested during larval and pupal stages are used in adult stage. Their utilization patterns are correlated with the activity levels of succinate (SDH) and glutamate (GDH) dehydrogenases and aspartate (AAT) and alanine (ALAT) aminotransferases and changes thereof were interpreted in terms of glycolytic oxidative phosphorylation and non-glycolytic transdeamination.  The trends in mass incorporation rates vis-à-vis enzyme activities indicated that the metabolism-related energy needs of all metamorphic events are majorly met through a gluconeogenetic mechanism called transdeamination, while the behavioural-related energy demands of larval and pupal stages are fulfilled through glycolytic-based oxidative phosphorylation. The activity trends further indicated that AAT plays major role in meeting the energy needs of larva and pupa, while GDH predominantly meets the energy requirements of reproduction in adults. The honey-enriched mulberry diet showed stage-specific and pathway-specific impacts on energy metabolism. It positively reinforced the energy metabolism in larval stage, but showed no significant effect in pupal and adult stages. Similarly, it showed more promising effect on glycolytic-oxidative phosphorylation and null or neutral effect on transdeamination

Title predictors of difficult subarachnoid block

Background: The aim of this prospective randomised study was to evaluate the association of four patient variables with difficulty in subarachnoid block. These variables are age, BMI, quality of bony landmarks and anatomical abnormality of spine. An attempt was made to prepare a difficulty score to predict this difficulty and assess it’s predictive value.Methods: 498 patients scheduled for surgical procedures under subarachnoid block were included. Each patient was assigned a difficulty score according to gradation of four variables. The difficulty was assessed in terms of number of levels, number of  attempts, and completeness of  anaesthesia. Since all these variables operated simultaneously and randomly in each patient, multivariate analysis was used with SPSS version 11.5. ROC curves were used to evaluate the sensitivity and specificity of the score. ROC curves were plotted at scores of 2, 3, 4 and 5 and AUC was compared.Results: This study was successful in preparing a score to predict difficulty in subarachnoid block. A score of 4 or more is indicative of difficulty as far as number of attempts and levels is concerned. However, completeness of anaesthesia could not be predicted with this score.Conclusions: This difficulty score can enable the anaesthetist to predict a difficult spinal puncture. This is a distinct advantage as multiple attempts are not without hazards

Identity Based Encryption and Data Self Destruction in Cloud Computing

When it comes to storing data, cloud storage is rapidly turning into the procedure for choice. Cloud storage is quickly becoming the strategy for decision. Putting away files remotely instead of by locally boasts an array of preferences for both home and professional clients. Cloud storage means “the storage of data online in the cloud”, however, the cloud storage is not completely trusted. Whether the data put away on cloud are in place or not turns into a significant concern of the clients also access control becomes a difficult job, particularly when we share data on cloud servers. To tackle this issue outsourcing Revocable IBE scheme for efficient key generation and key updating process is introduce. Also to improve the efficiency of cloud server in terms of storage new secure data self-destructing system in cloud computing is used. In this system, each cipher text (encrypted file) is labeled with a time interval. If the attributes associated with the cipher text satisfy the key’s access structure and both the time instant is in the allowed time interval then the cipher text is decrypted. After a user-specified end time the data at cloud server will be securely self-destructe

Evaluation of Fungicides, Soil Amendment Practices and Bioagents against Fusarium solani-Causal Agent of Wilt Disease in Chilli

Chilli is affected by the wilt disease caused by Fusarium solani, under irrigated conditions. In absence of resistant cultivars, the disease needs to be controlled by management practices. In vitro evaluation of six fungicides by Poisoned Food Technique showed that a combination of carbendazim+mancozeb was effective in inhibiting mycelial growth (93.6%), followed by Carbendazim alone (92.4%). In vivo soil drench using the same fungicides proved effective in controlling the pathogen. Integration of different treatments, including seedling dip, with Carbendazim, addition of vermicompost, drenching with fungicide, and application of Trichoderma viride was found to be effective in managing the disease, in comparison to individual treatments

Enumeration of Triangles in a Divisor Cayley Graph

In this paper a new class of arithmetic Cayley graphs, namely, divisor Cayley graphs associated with the divisor function d ƒvnƒw , n „d1,an integer is introduced. It is shown that this graph is regular, hamiltonian, connected and not bipartite, and when n is odd it is eulerian. The enumeration of triangles in this graph is also presentedKey words: Arithmetic graph, Cayley graph, Hamilton cycle, Fundamental triangle and Triangle