Adenosine to inosine editing by ADAR2 requires formation of a ternary complex on the GluR-B R/G site

RNA editing by members of the ADAR (adenosine deaminase that acts on RNA) enzyme family involves hydrolytic deamination of adenosine to inosine within the context of a double-stranded pre-mRNA substrate. Editing of the human GluR-B transcript is catalyzed by, the enzyme ADAR2 at the Q/R and R/G sites. We have established a minimal RNA substrate for editing based on the RIG site and have characterized the interaction of ADAR2 with this RNA by gel shift, kinetic, and cross-linking analyses. Gel shift analysis revealed that two complexes are formed on the RNA as protein concentration is increased; the ADAR monomers can be crosslinked to one another in an RNA-dependent fashion. We performed a detailed kinetic study of the editing reaction; the data from this study are consistent with a reaction scheme in which formation of an ADAR2.RNA ternary complex is required for efficient RNA editing and in which formation of this complex is rate determining. These observations suggest that RNA adenosine deaminases function as homodimers on their RNA substrates and may partially explain regulation of RNA editing in these systems

Nurses Alumni Association Bulletin, Fall 2009

2009 - 2010 Meeting Date Calendar 2010 Annual Luncheon & Meeting Notice Officers, Committee Chairs, Satellite and Volunteers Bulletin Publication Committee The President\u27s Message Treasurer\u27s Report Resume of Minutes Office News Committee Reports Annual Giving Contributors Letter to Alumni Janet C. Hindson Award Janet C. Hindson Award Qualifications 2009 Janet C. Hindson\u27s Nominees 2009 Janet C. Hindson\u27s Recipient\u27s Acceptance Speech TJUH\u27s MAGNET Award Our MAGNET Journey News About and From Our Graduates JSN Nursing Alumni Celebration; 117 years of Nursing Education Memories Ballad O\u27Brien Happy Birthday- To Be 80 or More Luncheon Pictures 50th Anniversary Class List for 1959 2009 Luncheon Reservations 50th Anniversary Class List for 1960 Love Ya Lorraine In Memoriam, Names of Deceased Graduates Class News - 2009 Additional Information - Pins, Transcript and Address Information Application For Nurses\u27 Scholarship Fund Certification Reimbursement Application Application For Relief Trust Fund Benefits List of Hotels Campus Map Change of Address Form - Donations - Information Change

The Mechanics of Fluid - Particle Systems

The study of the interaction of particles moving relative to fluids has had a long history and has led to many useful operations particularly in modern agriculture. Of special interest is the trajectory of particles and crop components but, until recently, it has not been possible to easily determine these. However with the recent publication of a trajectory plotting system and an associated monograph giving examples of its application, this need has now been met.Both should find ready application in education, research and design in the agricultural engineering industries. Copies of the trajectory plotting program and the associated monograph can be obtained at the addresses given in Section 4 below

High-efficiency GaAs concentrator space cells

High efficiency Al sub x Ga sub 1-x As/GaAs heteroface solar concentrator cells have been developed for space applications. The cells, which were grown using metalorganic chemical vapor deposition (MOCVD), have been fabricated in both the p-n and n-p configurations. Magnesium and zinc are used as the p-type dopants, and Se is used as the n-type dopant. The space cells, which are designed for use in a Cassegrainian concentrator operating at 100 suns, AMO, have a circular illuminated area 4 mm in diameter on a 5 mm by 5 mm cell. These cells have exhibited flash-tested efficiencies as high as 23.6 percent at 28 C and 21.6 percent at 80 C

Development of a New Lewis Acid-Catalyzed [3,3]-Sigmatropic Rearrangement: The Allenoate-Claisen Rearrangement

A new Lewis acid-catalyzed Claisen rearrangement has been developed that allows the stereoselective construction of β-amino-α,β,ε,ζ-unsaturated-γ,δ-disubstituted esters from simple allylic amines and allenoate esters. This reaction, which is contingent upon the use of Lewis acid, can be conducted with a range of metal salts (Yb(OTf)_3, AlCl_3, Sn(OTf)_2, Cu(OTf)_2, MgBr_2·Et_2O, FeCl_3, Zn(OTf)2) with catalyst loadings as low as 5 mol %. This catalytic process provides access to a diverse range of β-amino-α,β,ε,ζ-unsaturated-γ,δ-disubstituted esters in high yield and with excellent levels of diastereoselectivity for a series of allyl pyrrolidines (R_1 = H, Me, i-Pr, Ph, NR_2 = pyrrolidine, piperidine, Nme_2; ≥81% yield, ≥94:6 syn:anti) and allenoate esters (R_2 = H, Me, i-Pr, Ph, allyl, NPht, Cl; ≥75% yield, ≥91:9 syn:anti). The capacity of this new Claisen rearrangement to provide catalytic access to elusive structural motifs has also been demonstrated in the stereospecific formation of quaternary carbon bearing frameworks arising from geranyl- and neryl pyrrolidine (≥93% yield, >98:2 dr)

Rapid access to Asp/Glu sidechain hydrazides as thioester precursors for peptide cyclization and glycosylation

Head-to-sidechain macrocylic peptides, and neoglycopeptides, were readily prepared by site-specific amidation of aspartic and glutamic acid sidechain hydrazides. Hydrazides, serving as latent thioesters, were introduced through regioselective opening of the corresponding Na-Fmoc protected anhydride precursors

The Existence of Sterile Neutrino Halos in Galactic Centers as an Explanation of the Black Hole mass - Velocity Dispersion Relation

If sterile neutrinos exist and form halos in galactic centers, they can give rise to observational consequences. In particular, the sterile neutrinos decay radiatively and heat up the gas in the protogalaxy to achieve hydrostatic equilibrium, and they provide the mass to form supermassive blackholes. A natural correlation between the blackhole mass and velocity dispersion thus arises $\log(M_{BH,f}/M_{\odot})=\alpha \log (\sigma /200 {\rm km s^{-1}})+ \beta$ with $\alpha \approx 4$ and $\beta \approx 8$.Comment: Accepted in Ap

Expanding the scope of ligand substitution from [M(S2C2Ph2] (M = Ni2+, Pd2+, Pt2+) to afford new heteroleptic dithiolene complexes

The scope of direct substitution of the dithiolene ligand from [M(S2C2Ph2)2] [M = Ni2+ (1), Pd2+ (2), Pt2+ (3)] to produce heteroleptic species [M(S2C2Ph2)2Ln] (n = 1, 2) has been broadened to include isonitriles and dithiooxamides in addition to phosphines and diimines. Collective observations regarding ligands that cleanly produce [M(S2C2Ph2)Ln], do not react at all, or lead to ill-defined decomposition identify soft σ donors as the ligand type capable of dithiolene substitution. Substitution of MeNC from [Ni(S2C2Ph2)(CNMe)2] by L provides access to a variety of heteroleptic dithiolene complexes not accessible from 1. Substitution of a dithiolene ligand from 1 involves net redox disproportionation of the ligands from radical monoanions, –S•SC2Ph2, to enedithiolate and dithione, the latter of which is an enhanced leaving group that is subject to further irreversible reactions

Evaluation of sex differences in health-related quality of life outcomes associated with child abuse: Results from the Ontario Child Health Study

Aims. Despite the advances in child maltreatment research, there is still the need for comprehensive information about how abuse affects a broad range of categories of young adult functioning, and the extent to which these vary by sex. We examined the associations between child physical abuse (PA) and sexual abuse (SA) and six areas of functioning (mental health, physical health, life satisfaction, illegal substance use, alcohol problems and daily smoking). Methods. Data were obtained from the 1983 Ontario Child Health Study and follow-up in 2000/2001 (n = 1893). Multilevel regression estimated the adjusted associations for PA (with severity) and SA with each of the outcomes. Estimates with an entire sample were presented with sex-by-abuse interactions to examine sex differences and then presented separately by sex. Results. In the adjusted model, severe PA and SA were associated with impairment in mental health, and both forms of PA (severe and non-severe) and SA were associated with low life satisfaction. In addition, severe PA was associated with illegal substance use. Child abuse variables were not associated with poor physical health, alcohol problems or smoking. Although sex-stratified analyses revealed different patterns, there was no significant sex difference in the integrated sample. Conclusions. This is among the first community-based studies to show a strong association between child PA and SA and low life satisfaction in young adults. The abuse effects were similar for both sexes