Investigation of three classes of composite materials for space vehicle application

Three classes of composite materials for space vehicle applicatio

A Myelin Proteolipid Protein-LacZ Fusion Protein Is Developmentally Regulated and Targeted to the Myelin Membrane in Transgenic Mice

Transgenic mice were generated with a fusion gene carrying a portion of the murine myelin proteolipid protein (PLP) gene, including the first intron, fused to the E. coli LacZ gene. Three transgenic lines were derived and all lines expressed the transgene in central nervous system white matter as measured by a histochemical assay for the detection of β-galactosidase activity. PLP-LacZ transgene expression was regulated in both a spatial and temporal manner, consistent with endogenous PLP expression. Moreover, the transgene was expressed specifically in oligodendrocytes from primary mixed glial cultures prepared from transgenic mouse brains and appeared to be developmentally regulated in vitro as well. Transgene expression occurred in embryos, presumably in pre- or nonmyelinating cells, rather extensively throughout the peripheral nervous system and within very discrete regions of the central nervous system. Surprisingly, beta-galactosidase activity was localized predominantly in the myelin in these transgenic animals, suggesting that the NH_2-terminal 13 amino acids of PLP, which were present in the PLP-LacZ gene product, were sufficient to target the protein to the myelin membrane. Thus, the first half of the PLP gene contains sequences sufficient to direct both spatial and temporal gene regulation and to encode amino acids important in targeting the protein to the myelin membrane

Isotopic provenancing of Pb in Mitrovica, northern Kosovo: source identification of chronic Pb enrichment in soils, house dust and scalp hair

Mitrovica, northern Kosovo, is the site of some of the highest Pb concentrations reported in human populations; exemplified by Pb concentrations in scalp hair of up to 130 μg g-1 and widely-publicized of Pb-related ill-health and mortality amongst internally displaced populations. High human Pb burdens are accompanied by elevated concentrations of potentially harmful elements (PHEs) in soils and house dust within the city, which has a long history of mining and metallurgy. In this study enrichment-levels for PHEs in soils are quantified and compared to environmental quality guidelines and a statistically-derived estimation of background concentration. In addition, Pb isotopes (207Pb/206Pb, 208Pb/206Pb) are used to characterise the isotopic signatures of potential point sources of Pb and a mixing model employed to quantify the contribution of sources to Pb present in soils, house dust, and the scalp hair of children and young people. Pb isotopic evidence suggests that Pb in surface soils and house-dust is predominantly sourced from historical deposition of Pb-containing aerosols from metal smelting, with lower contributions from wind-blown dispersal of metalliferous waste. Pb present in scalp hair is interpreted as the result of non-occupational exposure and the ingestion and/or inhalation of Pb-enriched surface soil and house dust. This study represents one of the very few instances where this type of geochemical tracing technique has been successfully applied to definitively identify the source of Pb present within biological samples. The results of this study are of particular relevance to environmental management and highlight the human health risk posed by the legacy of now inactive mining and metallurgy in addition to the challenge posed in mitigating the risk posed by diffuse soil pollution. © 2015 Elsevier Ltd

Assessment of Metal Mining-Contaminated River Sediments in England and Wales

This report reviews how sediments and floodplain soils have been contaminated by abandoned metal mines and recommends what the Environment Agency should do about this issue

Reality check: Asynchronous instruction works!

Never before had I asked a student to cite an emoticon. In traditional classroom instruction, it is unlikely that this would have come up at all. However, in an asynchronous course, you never know where an online threaded discussion on citation formats will lead. As library educators, we have the opportunity to have an impact on a student\u27s chances for success in locating and managing information. We must draw upon the students\u27 familiarity with new technologies and teach them how to effectively articulate their information need, identify appropriate resources, evaluate what has been retrieved, and redirect their continued searching. The challenge that confronts us is compounded by the fact that many students enter the library only through a virtual door. In recognition of the shifting paradigm involving information and new technologies, Purdue University\u27s Electrical Engineering Technology (EET) program asked the Purdue Libraries to develop a credit course that would teach the students how to effectively locate, evaluate, and present information. The course, Information Strategies, has been a required course in EET was designed and taught by the libraries\u27 faculty since 1993. It has subsequently been adapted to other disciplines, as well. As evolution of new technologies continued, course instructors proposed the development of an asynchronous version of this course to the Indiana Higher Education Telecommunications System (IHETS). The development grant was awarded and the first Web-based version of this course was offered Spring 1999. The purpose of the IHETS course development grant was to enhance and convert [the Information Strategies course] to a digital format, which will allow asynchronous statewide access. In July 1998, the investigators, Professors Sheila Curl, Leslie Reynolds, Brent Mai, and Alexius Smith, began adapting the traditional course for delivery over the Internet