Seedbank reduction after different stale seedbed techniques in organic agricultural systems

Little information is available on the stale seedbed effect on seedbank reduction. This weed management is of increasing interest overall in organic agricultural systems where is no possible to use herbicides. The emergence dynamics and related seedbank reduction were evaluated following adoption of two different stale seedbed techniques (with or without irrigation), made during the spring-summer season in 2001 in organic agricultural systems. As expected, emergence was strongly stimulated by irrigation and soil tillage.When the no-tillage technique was adopted (control), the absence of soil disturbance resulted in extremely low emergence levels, associated with a reduction in the number of the relative species. Consequently, analysis of the residual seedbank of the shallow layer (0- 10 cm) of the control (no-till) showed only small reduction (about 1%). In contrast, the tillage-only experiment led to a reduction of about 5% in the same soil layer. However only with the irrigation, a drastic reduction in the amount of seeds (roughly half) was achieved. In particular, grasses showed the highest seedbank reduction rates. Despite this different effectiveness of the stale seedbed techniques, the soil layers at greater depths (10-20 and 20- 30 cm) were found to be completely unaffected, independently of the agronomic practices carried out. Qualitative analysis of exhumed seeds demonstrated that greatest "forcing of germination" (tillage + irrigation) resulted in a percentage increase of "deep-dormant" seeds as a consequence "non-dormant" seeds decrease. Although stale seedbed appeared to be only partially effective, we believe that if this agrotechnique is properly carried out and repeated at the appropriate times, it promises to be successful in agricultural systems where herbicides are excluded

Weed seedbank biodiversity in emmer wheat (triticum dicoccum (schrank) schübler) in a mountainous agro-ecological oasis (garfagnana, tuscany)

Phytocoenoses of conventional agroecosystems are subjected, already from several decades, to the reduction of the weed species present in the various crops. Such floristic decreasing is directly proportional to intensity of the agronomic impact. The present work is born from the hypothesis that the agro-ecological oases, managed with the ancient agrotechniques, are linked by an high degree of plant biodiversity. In this perspective it was carried out not only an analysis of the field emerged weeds, but even an evaluation of the seedbank since this one synthesizes the weed flora of a wider period. In the experimental agroecosystems, selected due to the typical Emmer wheat presence, an high degree of weed species diversity was observed, above all of terophytes, in the emerged flora as well in the seedbank. In both cases relative densities of each species were found low and without any weed dominance. Probably it occurs as a function of the high degree of competitive and allelopathic interactions. Almost scarce was the presence of exhumed seeds of graminaceae virtually due to their inability to store in the soil a persistent seedbank. Of particular importance it was the discovery of two rare species such as Agrostemma githago and Centaurea cyanus disappeared from many years by the landscape of “conventional” agricultural systems. The seedbank was found uniformly distributed in both sampled soil layers (0-15 and 15-30 cm) confirming that plowing induced an uniform burial of the annually produced seeds. The total examined soil profile (0-30 cm) showed a quantitative seedbank similar to those already found in “biological” agricultural systems (from 12.000 to 47.000 seeds m-2). However it was qualitatively formed even from several weed species of negligible agronomic impact as a function of their scarce competitivity like in the case of some caryophyllaceae (Silene noctiflora and S.alba), boraginaceae (Myosotis arvensis and Echium vulgaris) and campanulaceae (Legousia speculum-veneris). Finally both agronomic and ecological involvements of this weed complexity were discussed. This complexity was retained of crucial importance not only regards to the biodiversity conservation but even for their role in the landscape ecology. In synthesis, it has been confirmed the hypothesis that the ancient phytocoenoses are still present but only in these ecological oases in which the agronomic impact was ecologically sustainable. In conclusion, it was stressed the crucial role of these agro-ecological oases not only with the aim of the in situ biodiversity conservation, but even as ex situ germoplasma source to use in the perspective of ecological restoration of degraded areas

Agronomic-productive Characteristics of Two Genotype of Stevia Rebaudiana in Central Italy

Stevia rebaudiana Bertoni produces a variety of high-potency low calorie sweeteners in its' leaf tissue. The aim of this work was to evaluate the productive potential of two different Stevia rebaudiana genotypes and the characteristics of the production of the plant obtained through different cultivation methods, in central Italy. For several years (1992-2000) agronomic trials on Stevia rebaudiana cultivated in the littoral area near Pisa (Italy) carried out. In 1992 two different genotypes of Stevia (B1 and B2) transplanted in silt-loam soil; in 1996 other plants from B2 genotype, produced by vegetative process (micropropagation), were transplanted in the same field. In the period from 1992-1999 the production of leaves from both genotypes increase as of the third year and the ratio leaf-aerial plant, after the peak in the third year, tends to diminish. Except the first year (1997) there were not recorded statistically significant differences between the two propagation methods employed in relation to leaf production. The quantity of leaves produced from a single harvest was less than resulting from two cuttings and the micropropagation plants produced a larger amount of leaves than those from cutting. The leaf-stem ratio was to become an interesting morphological and production characteristic parameter of the plant. Stevia rebaudiana appears particularly suited for the cultivation environment of central Italy. A particular positive aspect that must be considered in these regions is that Stevia can be grown successfully as poliannual species because crop survival over the winter is high. The results obtained show that this species is economically profitable until the 5th or 6th year of cultivation. At our latitudes is also necessary to establish a program of genetic improvement in order to develop earlier varieties that can guaranty an optimum qualitative and quantitative seed production

Effects of Temperature, Light and Pre-Chilling on Seed Germination of Stevia rebaudiana (Bertoni) Bertoni Accessions

Stevia rebaudiana (Bertoni) Bertoni is a perennial shrub of the Asteraceae family native to Paraguay and Brazil where it has been used for several years as a sweetener. It is a short-day species, with a critical light requirement for flowering of roughly 13 hours. In plants whose biological cycle is strongly photoperiod-dependent, latitude is one of the major factors influencing reproduction. Late flowering may adversely affect seed production if this occurs during a season that is unfavorable to pollination. At Italian latitudes, this species often gives scanty seed production, with low germination rate and poor germination energy of seeds produced. In 2001 four accessions have been grown for seed production in a field plot experiment in Central Italy. The various accessions were found to exhibit noticeably different photoperiod requirements, which affected flowering time (from late August to the end of September) and consequently also the conditions of achene filling and ripening. Late flowering and seed ripening occurred during autumn season, unfavourable to complete seed formation, leading to an increase in the empty seed percentage recorded for each accession. Detailed germination trials were therefore undertaken using seeds collected from plants of the different accessions in order to assess the quality of the seeds produced. Various germination methods have been tested in a controlled environment adopting four different temperatures (20° C, 25° C constant temperature and 15/25° C, 20/30° C (16/8h) alternating temperature) in light or darkness with or without pre-chilling. Germination rates varied over an extensive range (germination percentage from 9 to 83%), mainly due to the divergent specific characteristics of the material examined and the different treatments studied. At all temperatures tested, the most earlier accession, showed the higher germination percentages (54-83%) while the latest accession was among those with the lowest germination values (9-44%). Overall, at the constant temperatures assayed, pre-chilling gave a higher germination percentage as compared to no pre-chilling, while at alternating temperatures higher germination rates were obtained in the absence of pre-chilling. The great variability observed between accessions for photoperiodic requirement as well as in seed germinative characteristics was probably due to the fact that this species was not subjected to any genetic improvement program

Guidelines for the use and interpretation of diagnostic methods in adult food allergy

Food allergy has an increasing prevalence in the general population and in Italy concerns 8 % of people with allergies. The spectrum of its clinical manifestations ranges from mild symptoms up to potentially fatal anaphylactic shock. A number of patients can be diagnosed easily by the use of first- and second-level procedures (history, skin tests and allergen specific IgE). Patients with complex presentation, such as multiple sensitizations and pollen-food syndromes, frequently require a third-level approach including molecular diagnostics, which enables the design of a component-resolved sensitization profile for each patient. The use of such techniques involves specialists' and experts' skills on the issue to appropriately meet the diagnostic and therapeutic needs of patients. Particularly, educational programs for allergists on the use and interpretation of molecular diagnostics are needed

Correction: Printed, cost-effective and stable poly(3-hexylthiophene) electrolyte-gated field-effect transistors

Correction for 'Printed, cost-effective and stable poly(3-hexylthiophene) electrolyte-gated field-effect transistors' by Davide Blasi et al., J. Mater. Chem. C, 2020, DOI: 10.1039/d0tc03342a

A Single-Molecule Bioelectronic Portable Array for Early Diagnosis of Pancreatic Cancer Precursors

A cohort of 47 patients is screened for pancreatic cancer precursors with a portable 96-well bioelectronic sensing-array for single-molecule assay in cysts fluid and blood plasma, deployable at point-of-care (POC). Pancreatic cancer precursors are mucinous cysts diagnosed with a sensitivity of at most 80% by state-of-the-art cytopathological molecular analyses (e.g., KRASmut DNA). Adding the simultaneous assay of proteins related to malignant transformation (e.g., MUC1 and CD55) is deemed essential to enhance diagnostic accuracy. The bioelectronic array proposed here, based on single-molecule-with-a-large-transistor (SiMoT) technology, can assay both nucleic acids and proteins at the single-molecule limit-of-identification (LOI) (1% of false-positives and false-negatives). It comprises an enzyme-linked immunosorbent assay (ELISA)-like 8 × 12-array organic-electronics disposable cartridge with an electrolyte-gated organic transistor sensor array, and a reusable reader, integrating a custom Si-IC chip, operating via software installed on a USB-connected smart device. The cartridge is complemented by a 3D-printed sensing gate cover plate. KRASmut, MUC1, and CD55 biomarkers either in plasma or cysts-fluid from 5 to 6 patients at a time, are multiplexed at single-molecule LOI in 1.5 h. The pancreatic cancer precursors are classified via a machine-learning analysis resulting in at least 96% diagnostic-sensitivity and 100% diagnostic-specificity. This preliminary study opens the way to POC liquid-biopsy-based early diagnosis of pancreatic-cancer precursors in plasma.</p

A Single-Molecule Bioelectronic Portable Array for Early Diagnosis of Pancreatic Cancer Precursors

A cohort of 47 patients is screened for pancreatic cancer precursors with a portable 96-well bioelectronic sensing-array for single-molecule assay in cysts fluid and blood plasma, deployable at point-of-care (POC). Pancreatic cancer precursors are mucinous cysts diagnosed with a sensitivity of at most 80% by state-of-the-art cytopathological molecular analyses (e.g., KRASmut DNA). Adding the simultaneous assay of proteins related to malignant transformation (e.g., MUC1 and CD55) is deemed essential to enhance diagnostic accuracy. The bioelectronic array proposed here, based on single-molecule-with-a-large-transistor (SiMoT) technology, can assay both nucleic acids and proteins at the single-molecule limit-of-identification (LOI) (1% of false-positives and false-negatives). It comprises an enzyme-linked immunosorbent assay (ELISA)-like 8 × 12-array organic-electronics disposable cartridge with an electrolyte-gated organic transistor sensor array, and a reusable reader, integrating a custom Si-IC chip, operating via software installed on a USB-connected smart device. The cartridge is complemented by a 3D-printed sensing gate cover plate. KRASmut, MUC1, and CD55 biomarkers either in plasma or cysts-fluid from 5 to 6 patients at a time, are multiplexed at single-molecule LOI in 1.5 h. The pancreatic cancer precursors are classified via a machine-learning analysis resulting in at least 96% diagnostic-sensitivity and 100% diagnostic-specificity. This preliminary study opens the way to POC liquid-biopsy-based early diagnosis of pancreatic-cancer precursors in plasma.</p