Prevalence, antimicrobial susceptibility and risk factors associated with non-typhoidal Salmonella on Ugandan layer hen farms

Abstract Background Non-typhoidal Salmonella (NTS) are among the leading global foodborne pathogens and a significant public health threat. Their occurrence in animal reservoirs and their susceptibilities to commonly used antimicrobials are poorly understood in developing countries. The aim of this study was to estimate the prevalence, determine antimicrobial susceptibility and identify risk factors associated with NTS presence in laying hen farms in Uganda through a cross-sectional study. Results Pooled faecal samples were collected from 237 laying hen farms and these were analysed for NTS following standard laboratory procedures. In total, 49 farms (20.7%; 95% Confidence interval (CI): 15.6–25.6%) were positive for NTS presence. Altogether, ten Salmonella serotypes were identified among the confirmed 78 isolates, and the predominant serotypes were Salmonella Newport (30.8%), S. Hadar (14.1%), S. Aberdeen (12.8%), S. Heidelberg (12.8%), and S. Bolton (12.8%). Phenotypic antimicrobial resistance was detected in 45(57.7%) of the isolates and the highest resistance was against ciprofloxacin (50.0%) followed by sulphonamides (26.9%) and sulphamethoxazole/trimethoprim (7.7%). Resistance was significantly associated with sampled districts (p = 0.034). Resistance to three or more drugs, multi-drug resistance (MDR) was detected in 12 (15.4%) of the isolates, 9 (75%) of these were from Wakiso district. A multivariable logistic model identified large farm size (OR = 7.0; 95% CI: 2.5–19.8) and the presence of other animal species on the farm (OR = 5.9; 95% CI: 2.1–16.1) as risk factors for NTS prevalence on farms. Having a separate house for birds newly brought to the farms was found to be protective (OR = 0,4; 95% CI: 0.2–0.8). Conclusion This study has highlighted a high prevalence and diversity of NTS species in laying hen farms in Uganda and identified associated risk factors. In addition, it has demonstrated high levels of antimicrobial resistance in isolates of NTS. This could be because of overuse or misuse of antimicrobials in poultry production. Also importantly, the insights provided in this study justifies a strong case for strengthening One Health practices and this will contribute to the development of NTS control strategies at local, national and international levels

Isolation of pathogenic bacteria from hospital staff apparel in Nigeria

A survey of bacteria contamination of hospital staff apparel in use in Anambra State, Nigeria, was carried out to determine the extent of contamination by clinically important bacteria. Of a total of 125 swab samples of hospital staff apparel, 72 (58%) showed bacterial contamination including 32 (70%) of 46 samples from hand gloves, 28 of 45 (62%) samples from protective gowns, and 12 of 34 (35%) samples from face-shields. The potentially pathogenic bacteria isolated were Salmonella spp, Proteus vulgaris, Shigella dysenteriae, Pseudomonas aeruginosaand Staphylococcus aureus. The isolation of clinically important bacteria from the apparel suggests the need for improved infection control measures.Malawi Medical Journal Vol. 17(4) 2005: 128-13

BACTERIOLOGICAL EVALUATION OF KWATA ABATTOIR WASTE WATER AWKA, NIGERIA

Abattoir wastes can have a detrimental effect on the environment, public health, animal health, and economy of a country if they are not effectively managed and controlled. The bacteriological evaluation of waste water from Kwata abattoir was carried out to determine the bacterial load present and if the waste water generated is suitable for direct discharge into the environment. A total of two samples were aseptically collected, in which the physicochemical analysis of the waste water showed objectionable color and odor, pH of 7.3 and 6.5, and temperature of 30.2°C and 25.3°C for samples A and B, respectively, of which the pH and temperature were within acceptable limits by WHO. The membrane filter method was used to determine the total coliform and thermotolerant coliform counts present per 100 mL of the samples using MacConkey agar and Eosin Methylene Blue Agar, respectively. The total viable count was obtained for both samples: 5.1×105 CFU/mL for sample A and 1.4×106 CFU/mL for sample B. Phenotypic and biochemical tests were carried out for four isolates, which include Escherichia coli, Salmonella spp., Bacillus spp., and Bacillus cereus. Thus, it can be concluded from the above study that untreated abattoir waste water contains a high level of pollutants, which supports the growth of the microbial population, as evidenced in the microbial study. Therefore, waste water has to be treated before discharge into the environment to protect public health and promote the safety of the environment

Facies, textural and geochemical evaluation of the post-Santonian sandstones in the Bida basin, Nigeria: Implications for control on hydrocarbon sandstone reservoir characteristics and paleoenvironments

Using a combination of field observations, textural analysis, scanning electron microscopy (SEM), mineralogical X-ray diffraction (XRD), X-ray fluorescence spectroscopy (XRF), and thin-section analysis, the hydrocarbon and paleoenvironmental characteristics of the Cretaceous sandstones of the southern Bida Basin, Nigeria, were evaluated. SEM data were used to display the mineral morphology, degree of cementation and presence of clay minerals on the grain surfaces and their pores on both sandstones of the Patti and Lokoja formations. The lithofacies analyses revealed three distinct facies associations. The XRD data showed that for the Lokoja and Patti sandstone reservoirs contained quartz from 49 to 67% and 43–68%, respectively. The XRF data also showed that both formations' sandstone reservoirs have a high percentage of silicon and low aluminum content. The grain size study showed that the Lokoja sandstone reservoirs include medium-coarse sandstone (−0.63 to 1.30 ф) that are poorly-moderately sorted (0.72–1.82 ф). In contrast, the Patti Formation is primarily made up of fine-medium sandstones at investigated outcrops, which typically display reasonably good to poor sorting (0.70–2.53 ф). The skewness of the sandstone reservoirs ranged from fine to coarse, and their kurtosis ranged from very platykurtic to leptokurtic. The research area's predominant depositional environments include shoreface/foreshore, estuary mudflat, and estuarine delta with fluvial interference, according to an analysis that combined sedimentary facies and textural data. This demonstrates that the sandstones from the two units have moderate quality prospective hydrocarbon reservoirs based on their physical and chemical characteristics