Pengembangan Waduk Cengklik Sebagai Kawasan Ekowisata Di Boyolali

Boyolali is a city in Central Java with a population density of 930,531 people / km with a land area of 1,015,101 km2. Boyolali has a diverse and more tourism potential compared to other cities in Central Java, one of which is the Cengklik Reservoir located in Ngargorejo and Sobokerto Villages, Ngemplak District, Boyolali. The selection of Cengklik Reservoir as a final project is due to the lack of progress that has increased significantly even though the natural potential that can be managed and maintained properly will run optimally. This development project is expected to be a benchmark for developing Cengklik Reservoir to be more developed, provide education for the community about efforts to preserve nature, increase economic income for local residents as jobs, attract tourists to be more interested in visiting the reservoir, and meet quality standards reservoir as an ecotourism area in Boyolali

Analisis Postur Kerja Menggunakan Metode Posture Ergonomic Risk Assessment (PERA) Untuk Meminimalisir MSDs (Studi Kasus : UKM Robin Gitar Sukoharjo)

The process of making acoustic guitar products in Sukoharjo has 4 work stations and 17 work activities. During the production process there is a risk of musculoskeletal disorders (MSDs) because the work is done for 8 hours in a sitting position with the body bent, squatting, and doing repetitive or repetitive movements, especially the upper body such as the neck, shoulders, back, arms, and wrists. . The purpose of this study was to identify and analyze posture conditions using the Nordic Body Map (NBM) and Posture Ergonomic Risk Assessment (PERA) methods. The NBM method includes complaints of pain in all parts of the worker's body. The PERA method is used to determine muscle fatigue that occurs in each part of the worker's body by determining work posture, work duration, and work movement frequency. The results of the study using the NBM method were carried out on all workers, there were 2 high-risk workers, 3 medium-risk workers, and 7 low-risk workers. Efforts to minimize the risk of MSDs were redesigned using SolidWork and CATIA applications. The results of the study using the PERA method showed that there were 5 very high and high priority workers on the neck, back, shoulders, right arm, wrist and leg. The improvement proposals given to reduce the level of risk felt by workers are improving work facilities, OHS training and doing regular muscle stretching and exercise

Wonogiri Convention and Exhibition Center

Wonogiri Convention And Exhibition Center is a building that becomes a center for organizing activities to discuss common needs carried out by many people relating to conferences, meetings and exhibitions in Kabupaten Wonogiri which emphasize the Neo-Vernacular Architecture approach with a more advanced contemporary design, varied, flexible, and innovative. The success of Surakarta city is one of the cities of MICE which is expected to be spread to the surrounding areas, especially Kabupaten Wonogiri. Organizing conventions and exhibitions is expected to be a dynamic for industry and economic development. However, in Wonogiri district itself there are no facilities that are able to accommodate convention and exhibition activities comfortably. The potential of Kabupaten Wonogiri for the future prospects of the Wonogiri Convention And Exhibition Center is expected to be able to overcome the obstacles that exist for the development and progress of Kabupaten Wonogiri

Mitochondria-Specific Accumulation of Amyloid β Induces Mitochondrial Dysfunction Leading to Apoptotic Cell Death

Mitochondria are best known as the essential intracellular organelles that host the homeostasis required for cellular survival, but they also have relevance in diverse disease-related conditions, including Alzheimer's disease (AD). Amyloid β (Aβ) peptide is the key molecule in AD pathogenesis, and has been highlighted in the implication of mitochondrial abnormality during the disease progress. Neuronal exposure to Aβ impairs mitochondrial dynamics and function. Furthermore, mitochondrial Aβ accumulation has been detected in the AD brain. However, the underlying mechanism of how Aβ affects mitochondrial function remains uncertain, and it is questionable whether mitochondrial Aβ accumulation followed by mitochondrial dysfunction leads directly to neuronal toxicity. This study demonstrated that an exogenous Aβ1–42 treatment, when applied to the hippocampal cell line of mice (specifically HT22 cells), caused a deleterious alteration in mitochondria in both morphology and function. A clathrin-mediated endocytosis blocker rescued the exogenous Aβ1–42-mediated mitochondrial dysfunction. Furthermore, the mitochondria-targeted accumulation of Aβ1–42 in HT22 cells using Aβ1–42 with a mitochondria-targeting sequence induced the identical morphological alteration of mitochondria as that observed in the APP/PS AD mouse model and exogenous Aβ1–42-treated HT22 cells. In addition, subsequent mitochondrial dysfunctions were demonstrated in the mitochondria-specific Aβ1–42 accumulation model, which proved indistinguishable from the mitochondrial impairment induced by exogenous Aβ1–42-treated HT22 cells. Finally, cellular toxicity was directly induced by mitochondria-targeted Aβ1–42 accumulation, which mimics the apoptosis process in exogenous Aβ1–42-treated HT22 cells. Taken together, these results indicate that mitochondria-targeted Aβ1–42 accumulation is the necessary and sufficient condition for Aβ-mediated mitochondria impairments, and leads directly to cellular death rather than along with other Aβ-mediated signaling alterations

Glycolate Oxidase Isozymes Are Coordinately Controlled by GLO1 and GLO4 in Rice

Glycolate oxidase (GLO) is a key enzyme in photorespiratory metabolism. Four putative GLO genes were identified in the rice genome, but how each gene member contributes to GLO activities, particularly to its isozyme profile, is not well understood. In this study, we analyzed how each gene plays a role in isozyme formation and enzymatic activities in both yeast cells and rice tissues. Five GLO isozymes were detected in rice leaves. GLO1 and GLO4 are predominately expressed in rice leaves, while GLO3 and GLO5 are mainly expressed in the root. Enzymatic assays showed that all yeast-expressed GLO members except GLO5 have enzymatic activities. Further analyses suggested that GLO1, GLO3 and GLO4 interacted with each other, but no interactions were observed for GLO5. GLO1/GLO4 co-expressed in yeast exhibited the same isozyme pattern as that from rice leaves. When either GLO1 or GLO4 was silenced, expressions of both genes were simultaneously suppressed and most of the GLO activities were lost, and consistent with this observation, little GLO isozyme protein was detected in the silenced plants. In contrast, no observable effect was detected when GLO3 was suppressed. Comparative analyses between the GLO isoforms expressed in yeast and the isozymes from rice leaves indicated that two of the five isozymes are homo-oligomers composed of either GLO1 or GLO4, and the other three are hetero-oligomers composed of both GLO1 and GLO4. Our current data suggest that GLO isozymes are coordinately controlled by GLO1 and GLO4 in rice, and the existence of GLO isozymes and GLO molecular and compositional complexities implicate potential novel roles for GLO in plants

Receptor Heteromerization Expands the Repertoire of Cannabinoid Signaling in Rodent Neurons

A fundamental question in G protein coupled receptor biology is how a single ligand acting at a specific receptor is able to induce a range of signaling that results in a variety of physiological responses. We focused on Type 1 cannabinoid receptor (CB1R) as a model GPCR involved in a variety of processes spanning from analgesia and euphoria to neuronal development, survival and differentiation. We examined receptor dimerization as a possible mechanism underlying expanded signaling responses by a single ligand and focused on interactions between CB1R and delta opioid receptor (DOR). Using co-immunoprecipitation assays as well as analysis of changes in receptor subcellular localization upon co-expression, we show that CB1R and DOR form receptor heteromers. We find that heteromerization affects receptor signaling since the potency of the CB1R ligand to stimulate G-protein activity is increased in the absence of DOR, suggesting that the decrease in CB1R activity in the presence of DOR could, at least in part, be due to heteromerization. We also find that the decrease in activity is associated with enhanced PLC-dependent recruitment of arrestin3 to the CB1R-DOR complex, suggesting that interaction with DOR enhances arrestin-mediated CB1R desensitization. Additionally, presence of DOR facilitates signaling via a new CB1R-mediated anti-apoptotic pathway leading to enhanced neuronal survival. Taken together, these results support a role for CB1R-DOR heteromerization in diversification of endocannabinoid signaling and highlight the importance of heteromer-directed signal trafficking in enhancing the repertoire of GPCR signaling

Inhibition of Dengue Virus Entry and Multiplication into Monocytes Using RNA Interference

Prevention and treatment of dengue infection remain a serious global public health priority. Extensive efforts are required toward the development of vaccines and discovery of potential therapeutic compounds against the dengue viruses. Dengue virus entry is a critical step for virus reproduction and establishes the infection. Hence, the blockade of dengue virus entry into the host cell is an interesting antiviral strategy as it represents a barrier to suppress the onset of infection. This study was achieved by using RNA interference to silence the cellular receptor, and the clathrin mediated endocytosis that enhances the entry of dengue virus in monocytes. Results showed a marked reduction of infected monocytes by flow cytometry. In addition, both intracellular and extracellular viral RNA load was shown to be reduced in treated monocytes when compared to untreated monocytes. Based on these findings, this study concludes that this therapeutic strategy of blocking the virus replication at the first stage of multiplication might serve as a hopeful drug to mitigate the dengue symptoms, and reduction the disease severity