Helianthus tuberosus (Jerusalem artichoke) tubers improve glucose tolerance and hepatic lipid profile in rats fed a high-fat diet

Objectives: To analyze the effects of feeding Helianthus tuberosus (HT) tubers on glucose tolerance and lipid profile in rats fed a high-fat diet (HFD). Methods: A normal HFD or HFD including 10 w/w% HT tubers (HFD + HT) was fed to F334/Jcl rats. After 10 weeks, organ weights, glucose tolerance, and lipid profile were analyzed. Results: The body weight, liver weight, and epidermal fat content in the HFD group were higher than those of the normal group, and similar to those of the HFD + HT group. The oral glucose tolerance test at 10 weeks revealed that the blood glucose level 30 min after beginning the test in the HFD + HT group was significantly lower than that in the HFD group. Liver triglyceride and total cholesterol levels in the HFD + HT group were significantly lower than those in the HFD group. Fecal triglyceride and total cholesterol levels in the HFD + HT group were higher than those in the HFD group. Histological analyses revealed that fat and glycogen accumulation increased in the HFD group, but decreased in the HFD + HT group. Conclusions: These results indicate that HT tubers have anti-fatty liver effects based on improvements in glucose tolerance and the hepatic lipid profile

行政主体の運動教室が住民主体の自主グループへと移行する過程における保健師の役割

筆者らは、短期大学部専攻科公衆衛生看護学専攻の実習において、「運動習慣の普及による健康づくり、介護予防の推進」を事業の柱とした行政主体の運動教室に準備段階から参加した。その活動の中で、教室の運営や住民主体の自主グループとなる移行過程を学び、その過程における保健師の支援のあり方を考察した。保健師として自主グループ化を推進するためには、教室のプログラム内容の充実を図り、参加者の継続参加を促す必要がある。そして、保健師はキーパーソンとなり得る参加者、健康づくり推進員を見定めて、その主体性を尊重し、自分自身の役割を徐々に相談者的役割に移行することが重要である

Interlaboratory control among INCO-DEV MYCOTOX PROJECT LABORATORIES

The Work Package 1 “ Development and standardization of effective analytical tools for mycotoxin (aflatoxins B1, B2  G1, G2  ochratoxin A, zearalenone, fumonisin B1, B2  and tricothecenes) determination in wheat and maize”  aim to implement the interlaboratory control between the partners laboratories from Brazil, Uruguay, Chile and Argentina as part of the objectives of INCO-DEV MYCOTOX PROJECT 2003-2005  “The Development of a Food Quality Management System for the Control of Mycotoxins in cereal Production and Processing Chains in Latin America South Cone Countries”.  The ojectives of the interlaboratory control were: evaluate the performance of the laboratories and the main difficulties encountered in performing the analytical procedure for mycotoxins  determination in maize and wheat; contribute to the harmonization of analytical procedures of the partners laboratories and contribute to the laboratory’s proficiency in mycotoxin analysis.  Maize reference materials for aflatoxins and zearealenone were prepared and used to the implementation of the interlaboratory control.  In summary, the preparation of these samples involved: milling (<20 mesh), homogeneization, analysis to verify the homogeneity of the bulk material and packing (labelled vacuum “sachets” or plastic bottles) and mycotoxin analysis.  The homogeneity of the material was investigated by the analysis of variance – ANOVA- according to International Harmonized Protocol for the Proficiency testing of (Chemical)Analytical Laboratories as established by ISO 43-1 – Annex at 95% of confidence level by calculating an F-statistic ans Ss/ÿ (ÿ =15%). All batches of test material were stored under – 18ºC and protected from light prior to and after packaging.  Aflatoxins in the test materials were determinated by immunoaffinity with liquid chromatography (LC) with pos-column derivatization and thin layer chromatography (TLC).  Zearalenone in the test materials were determined by solid phase column (Romer 224TM) with LC.  Four homogeneus maize materials were prepared: blank for zearalenone, blank for aflatoxins and two naturally contaminated for aflatoxins.  These samples were used to validated analytical methods and as reference samples for proficiency test.  The participating laboratories received refrigerated parcel containing: coded maize samples and blank for spiking purpose, test material receipt form, additional instructions, results reporting sheets and analytical work questionnaire in 3 rounds.  The results were evaluated by using z-score function being calculated considering the best value representing the true measure of mycotoxin in the sample (as per evaluation in the homogeneity tests).  Additional FAPAS test material for mycotoxins were purchased in order to assess the laboratory performance and to validated the reference materials.  In case of “questionable” or “unsatisfactory” results, the Laboratory were advised to treate them as non conforming work, make the necessary modifications and adjustments in the methods, taking into account the method performance criteria (CEN).  The laboratories were strongly recommended to write a report containing the analysis of the causes and correctives actions proposed, giving special attention to: correct use of calibrated pipettes; chromatographic condition including the calibration curve and injected volume of extract and standard

Informe de calidad e inocuidad de trigo uruguayo : caracterización en calidad de la zafra de trigo 2013/2014

El siguiente trabajo es una iniciativa del MGAP-DGSSAA Plan Nacional de Silos que cuenta con la colaboración de la Mesa Nacional de Trigo, para caracterizar anualmente la zafra de trigo en un esfuerzo por conocer qué trigos producimos y crear una historia de nuestra producción

Informe de calidad e inocuidad de trigo Uruguayo

El siguiente trabajo es una caracterización de la zafra de trigo 2019-2020, iniciativa del MGAP-DGSA que cuenta con la colaboración de la Mesa Nacional de Trigo. La intención es caracterizar anualmente la zafra de trigo y generar datos históricos, para conocer qué trigos producimos e identificar tendencias de cambio en nuestra producción a través del tiempo