Candida albicans Potassium Transporters

Potassium is basic for life. All living organisms require high amounts of intracellular potassium, which fulfils multiple functions. To reach efficient potassium homeostasis, eukaryotic cells have developed a complex and tightly regulated system of transporters present both in the plasma membrane and in the membranes of internal organelles that allow correct intracellular potassium content and distribution. We review the information available on the pathogenic yeast Candida albicans. While some of the plasma membrane potassium transporters are relatively well known and experimental data about their nature, function or regulation have been published, in the case of most of the transporters present in intracellular membranes, their existence and even function have just been deduced because of their homology with those present in other yeasts, such as Saccharomyces cerevisiae. Finally, we analyse the possible links between pathogenicity and potassium homeostasis. We comment on the possibility of using some of these transporters as tentative targets in the search for new antifungal drugs

Direct and indirect influences of objective socioeconomic position on adolescent health: the mediating roles of subjective socioeconomic status and lifestyles

The use of composite indices and subjective measures to evaluate socioeconomic position, taking into account the effect of inequalities on adolescent health-related behaviors, can contribute to understanding the effect of inequalities on health during adolescence. The aim of this study was to examine the direct and indirect contribution of objective and subjective socioeconomic factors in a broad range of health and lifestyles outcomes. The data come from a representative sample of adolescents (N = 15,340; M age = 13.69) of the Health Behavior in School-aged Children study in Spain. Structural equation modeling was used for data analysis. A global index for evaluating objective socioeconomic position predicted both health and healthy lifestyles. Subjective socioeconomic status mediated the relationship between objective socioeconomic position and health but did not have a significant effect on healthy lifestyles when objective indicators were considered. Lastly, fit indices of the multiple-mediator model—including the direct effect of objective socioeconomic position on health and its indirect effects through the subjective perception of wealth and lifestyles—explained 28.7% of global health variance. Interventions aimed at reducing the impact of health inequalities should address, in addition to material deprivation, the psychological and behavioral consequences of feeling poor.Consejería de Economía, Innovación y Ciencia de la Junta de Andalucía P11—SEJ—800

Debaryomyces hansenii Is a Real Tool to Improve a Diversity of Characteristics in Sausages and Dry-Meat Products

Debaryomyces hansenii yeast represents a promising target for basic and applied biotechnological research It is known that D. hansenii is abundant in sausages and dry-meat products, but information regarding its contribution to their characteristics is blurry and contradictory. The main goal in this review was to define the biological contribution of D. hansenii to the final features of these products. Depending on multiple factors, D. hansenii may affect diverse physicochemical characteristics of meat products. However, there is general agreement about the significant generation of volatile and aromatic compounds caused by the metabolic activities of this yeast, which consequently provide a tendency for improved consumer acceptance. We also summarize current evidence highlighting that it is not possible to predict what the results would be after the inoculation of a meat product with a selected D. hansenii strain without a pivotal previous study. The use of D. hansenii as a biocontrol agent and to manufacture new meat products by decreasing preservatives are examples of exploring research lines that will complement current knowledge and contribute to prepare new and more ecological products

Análisis de clúster de la actividad física y las conductas sedentarias de los adolescentes españoles, correlación con la salud biopsicosocial

This study is part of the 2006 edition of the Health Behaviour in School-Aged Children (HBSC) study. The data presented was drawn from a sample of 21,811 Spanish adolescents ranging from 11 to 18 years of age. Through a cluster analysis, General Lineal Model, significance and effect size tests, we obtained relevant information about the physical activity habits and sedentary behaviours of Spanish adolescents. It is evident that there is a risk situation in older adolescents, especially girls. These results have the advantage of relating to a global health score; clear indicators of the advantages of an active lifestyle for adolescents were obtained. Furthermore, these data provide information that is relevant to the displacement hypothesis, which is widely discussed in the scientific literature. In short, this paper provides interesting keys for theoretical debate and practical action in the field of physical activity during adolescence

Host cell type-dependent translocation and PhoP-mediated positive regulation of the effector SseK1 of Salmonella enterica.

Salmonella enterica expresses two virulence-related type III secretion systems (T3SSs) encoded in Salmonella pathogenicity island 1 (SPI1) and SPI2, respectively. SseK1 is a poorly characterized substrate of the SPI2-encoded T3SS. Here, we show that this effector is essential to get full virulence both in oral and intraperitoneal mice infections, in spite of not having a role in invasion or intracellular proliferation in cultured mammalian cells. In vitro, expression of sseK1 was higher in media mimicking intracellular conditions, when SPI2 was induced, but it was also significant under SPI1 inducing conditions. A detailed analysis of translocation of SseK1 into host cells unveiled that it was a substrate of both, T3SS1 and T3SS2, although with different patterns and kinetics depending on the specific host cell type (epithelial, macrophages, or fibroblasts). The regulation of the expression of sseK1 was examined using lacZ and bioluminescent lux fusions. The two-component system PhoQ/PhoP is a positive regulator of this gene. A combination of sequence analysis, directed mutagenesis and electrophoretic mobility shift assays showed that phosphorylated PhoP binds directly to the promoter region of sseK1 and revealed a PhoP binding site located upstream of the predicted -35 hexamer of this promoter

Proceedings of Sinn und Bedeutung 21

TesisSe realizó un análisis clínico-electrocardiográfico integral de los hemibloqueos comprendiendo incidencia, edad, etiología, evaluación cuantitativa de los criterios diagnósticos, relación con los trastornos de conducción aurículo-ventricular, y pronóstico. Con tal motivo se estudiaron 221hemibloqueos encontrados en 7,130 pacientes adultos de sexo masculino en un servicio de cardiología y medicina. Los hemibloqueos fueron diagnosticados mediante los criterios señalados por Rosenbaum, Castellanos, y Prior y Blount

Efectos del apego, la fiabilidad y la desconfianza sobre la calidad de relación: propuesta de un modelo de medida en entornos tecnológicos.

La calidad de relación es un concepto profusamente analizado en la literatura de marketing,destacando entre tales trabajos aquellos dirigidos a establecer tanto los antecedentes como los resultados asociados a la misma.Una vez que determinado el papel jugado como antecedentes de la calidad de relación por parte delos esfuerzos relacionales y la inversión relacional percibida, se deben ampliar los estudios a otros tipos de variables, como pueden ser diversas variables cognitivas. Dentro de las mismas, el presente trabajo analiza el papel jugado por los componentes del apego (autonomía, cercanía y competencia)como antecedentes de la calidad de relación, así como el impacto que los componentes de la fiabilidad (competencia y benevolencia) tengan sobre confianza y desconfianza. Si bien se ha documentado el impacto que la calidad de relación presenta sobre las distintas manifestaciones de la lealtad, resulta interesante estudiar el efecto que puedan presentar con respecto a otras variables como el procesamiento de información negativa.El presente trabajo presenta el resultado de un pre-test llevado a cabo para depurar una escala de medición relativa a los constructos y relaciones antes planteados entre usuarios de equipos informáticos bajo entornos operativos diferentes

Heterogeneous resource management in energy hubs with self-consumption: Contributions and application example

The energy hub concept and modeling methodology are widely employed tools for solving resource conversionand storage scheduling problems. For instance, industrial clusters might benefit from determining the suitabletime to operate their facilities and to sell electricity to the public power grid, according to legal, economic orenvironmental factors. In this paper, novel elements are introduced in order to more accurately represent realplants and to reduce the amount of decision variables. The major innovation is to consider devices consuming aresource which is not related to the quantity of output produced, by attaching binary decision variables tocertain energy hub outputs. Secondly, a path vector is defined to take into account the flows of resources withinthe system instead of employing a variable for each branch between the components. The third innovationconsists of an additional vector to express the amount of output resources sold from the energy hub, includingconstraints for those resources which are exported and imported through the same medium. An extended energyhub model is first proposed and then applied to a real plant example, including multiple and heterogeneousresources and performing a comparison between days with different demands, weather conditions and electricityprices. The results obtained in the selected scenarios demonstrate a logical operation scheduling, and thereforevalidate the proposed approach

Iguales, familia y participación en actividades deportivas organizadas durante la adolescencia

Suplemento de artículos seleccionados con revisión del Congreso Andaluz de Psicología de la Actividad Física y el Deporte (13 : 2011 : Sevilla). Editores : José Carlos Caracuel ; Rocío Bohórquez.This study aimed to examine the influence of sport activities by peer groups and the family on the frequency of adolescents' participation in organized sport activities. A sample of 9,821 Spanish adolescent schoolchildren between the ages of 11 and 18 from the 2010 edition of the international study on Health Behaviour in School-Aged Children (HBSC) was used. The results obtained by means of an Answer Tree multivariate analysis indicated that gender was the best predictor of organized sport activity, with participation being notably higher in boys. As for the role of other variables, the practice of sport in peer and family activities were the most influential variables for boys. On the contrary, in the girls' case, age appeared as the first factor and the influence of sport in family activities and the peer group was different depending on age. In conclusion, family and peers seem to be significant influences on involvement in sport, despite having different roles according to the adolescents' gender and age