Respiratory evaluation through volumetric capnography among grade III obese and eutrophic individuals: a comparative study

sem informaçãoExcess trunk body fat in obese individuals influences respiratory physiological function. The of this study were to compare volumetric capnography findings (VCap) between severely obese patients and normal-weight subjects and to assess whether there is a1372177183sem informaçãosem informaçãosem informaçã

Cercosporin quantification in Cercospora coffeicola isolates by spectrophotometry and high performance liquid chromatography: a comparative analysis

info:eu-repo/semantics/publishedVersio

Avaliação da atividade mutagênica do extrato etanólico bruto de Paepalanthus latipes (Eriocaulaceae) e dos compostos flavonoídicos 7-metoxilados relacionados

Paepalanthus latipes (Silveira) é uma das plantas brasileiras nativas da Serra do Cipó, Minas Gerais, conhecida como sempre-vivas e muito exportada para os Estados Unidos, Europa e Japão, por seu valor ornamental. Poucos resultados são relatados quanto à atividade biológica destas plantas. Este trabalho teve como objetivo avaliar a atividade mutagênica do extrato etanólico bruto de P. latipes e de dois flavonóides 7-metoxilados dele isolados (7-metilquercetagetina e 7-metilquercetagetina-4'-O-β-D-glicopiranosídeo), através do teste de Ames, utilizando linhagens mutantes de Salmonella typhimurium TA98 e TA100. A atividade mutagênica das amostras testadas, em concentrações variando de 62,5 a 375 μg/placa, foi determinada com ativação metabólica (utilizando-se a fração microssomal S9) e na ausência dessa. Os resultados demonstraram que, tanto o extrato etanólico bruto de P. latipes quanto os flavonóides 7-metoxilados testados, não induziram atividade mutagênica nas linhagens TA98 e TA100.Paepalanthus latipes (Silveira) is one of the native Brazilian plants from Serra do Cipó, Minas Gerais, known as sempre-vivas which is exported to the United States, Europe and Japan because of its ornamental value. Few results were reported in relation to the biological activity of these plants. In this work, the mutagenicity of the crude ethanolic extract of P. latipes and isolated 7-methoxyflavonoids (7-methylquercetagetin and 7-methylquercetagetin-4'-O-β-D-glucopyranoside) was evaluated by using the Ames test with Salmonella typhimurium TA98 and TA100 strains, at concentrations from 62.5 to 375 μg/plate, with and without metabolic activation (using microsomal S9 fraction). Results demonstrated that both the extract of P. latipes as well as the tested 7-methoxyflavonoids did not induce mutagenicity at the experimental tested conditions

Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis

Cercosporin has excellent properties of photosensitization that have been widely used in organophotocatalyst and photodynamic therapy as well as an antimicrobial agent. Therefore, there is a need to quantify it accurately with accessible methods. A comparative analysis of cercosporin quantification obtained by spectrophotometry (SPEC) and high-performance liquid chromatography (HPLC) was performed for nineteen Cercospora coffeicola isolates from different coffee-producing municipalities in Brazil. Image analysis of cercosporin crystals was performed in isolates with either high or low production of the toxin. Our results show that SPEC and HPLC are equally valid for the cercosporin evaluation of C. coffeicola cultures grown in vitro. The isolates with high cercosporin production had a higher crystal number and size when compared to the one with low cercosporin productioninfo:eu-repo/semantics/publishedVersio

5. Impacto del cambio climático en la sedimentación y en la acumulación de carbono en los lagos de la Amazonia peruana

Los suelos, las aguas y los sedimentos de la cuenca amazónica son importantes compartimentos del ciclo de carbono, el cual se encuentra parcialmente almacenado en los bosques inundables y en los lagos de inundación de la región (20% de la superficie total de la cuenca). Estudiar los procesos de sedimentación lacustres a diferentes escalas espaciales y de tiempo permite mejorar le estimación del balance biogeoquímico del carbono (uno de los gases de efecto Invernadero responsable del cambio climático actual, el CO2). Las tasas de sedimentación en las áreas de inundación dependen de varios factores, como el tipo de agua de los lagos y del tipo de conexión que tienen con los ríos. El análisis en laboratorio de muestras de sedimentos, que mantienen la secuencia de deposición, sirve para determinar las condiciones geológicas, meteorológicas, los cambios en el ecosistema e incluso acciones de origen humano que pueden haber afectado los regímenes de sedimentación y la composición de los sedimentos a lo largo del tiempo.Les sols, les eaux et les sédiments du bassin amazonien sont des compartiments importants du cycle du carbone, qui est partiellement stocké dans les forêts et les lacs inondés dans la région (20% de la superficie totale du bassin). Connaître les processus de sédimentation lacustres à différentes échelles spatiales et temporelles permet d'améliorer l'estimation de l'équilibre biogéochimique du carbone (un des gaz responsables du changement climatique actuel, CO2). Les taux de sédimentation dans la plaine inondable dépendent de plusieurs facteurs, y compris le type d'eau des lacs et le type de connexion avec les rivières. Les analyses de laboratoire des échantillons de sédiments, qui maintiennent la séquence de dépôt, sont utilisées pour déterminer les caractéristiques géologiques, les conditions météorologiques, les changements dans l'écosystème et même les actions humaines qui peuvent avoir une incidence sur les régimes de sédimentation et la composition des sédiments au fil du temps.The soils, waters and sediments of the Amazon basin are important compartments of the carbon cycle, which are partially stored in flooded forests and lakes of flooding in the region (20% of the total basin area). The study of lacustrine sedimentation processes at different spatial and temporal scales allows improving the estimation of biogeochemical carbon balance (one of the greenhouse gases responsible for the current climate change, CO2). Sedimentation rates in flood areas depend on several factors, such as the type of water from lakes and the type of connection this water has with rivers. The laboratory analysis of sediment samples, which maintain the deposition sequence, is used to determine geological and meteorological conditions; as well as changes in the ecosystem, even actions arising from humans that may have affected the sedimentation regimes and sediment composition along the time

Spin Glass Behavior in RuSr2Gd1.5Ce0.5Cu2O10

The dynamics of the magnetic properties of polycrystalline RuSr2Gd1.5Ce0.5Cu2O10 (Ru-1222) have been studied by ac susceptibility and dc magnetization measurements, including relaxation and ageing studies. Ru-1222 is a reported magneto-superconductor with Ru spins magnetic ordering at temperatures near 100 K and superconductivity in Cu-O2 planes below Tc ~ 40 K. The exact nature of Ru spins magnetic ordering is still debated and no conclusion has been reached yet. In this work, a frequency-dependent cusp was observed in ac susceptibility vs. T measurements, which is interpreted as a spin glass transition. The change in the cusp position with frequency follows the Vogel-Fulcher law, which is commonly accepted to describe a spin glass with magnetically interacting clusters. Such interpretation is supported by themoremanaent magnetization (TRM) measurements at T = 60 K. TRM relaxations are well described by a stretched exponential relation, and present significant ageing effects.Comment: 4 pages, 6 figures, submitted to Phys. Rev.

4. HYBAM: un observatorio para medir el impacto del Cambio Climático sobre la erosión y los flujos de sedimentos en la zona Andino-Amazónica

La cuenca Amazónica es la más grande del mundo. La instalación del observatorio HYBAM con una amplia red de estaciones hidrológicas ubicadas desde el piedemonte andino hasta el océano Atlántico permite, desde el 2003, la generación de registros periódicos y confiables (nivel del agua, caudal, concentración de sedimentos y otros parámetros físico-químicos de la calidad del agua) a lo largo de toda la cuenca. Hoy en día, el desarrollo de técnicas satelitales, como la altimetría, permite completar eficientemente los datos obtenidos por las redes de medición en los ríos. El recrudecimiento de eventos extremos en la Amazonia (Inundaciones, sequias,) por efecto del cambio climático actual asociado a un cambio acelerado de ocupación de los suelos (deforestación, prácticas agrícolas), tiene una incidencia directa sobre la producción sedimentarla. Conocer los flujos de materiales transportados por los ríos es esencial tanto para la navegación fluvial como para guiar la explotación de los recursos naturales (agua, petróleo, minerales), para conocer el transporte de partículas contaminantes o para diseñar infraestructuras.Le bassin de l’Amazone est le plus grand du monde. La mise en œuvre de l’observatoire HYBAM avec un vaste réseau de stations hydrologiques situées sur les contreforts des Andes jusqu’á l’océan Atlantique permet de générer, depuis 2003, des données régulières et fiables tout le long du bassin (hauteur d’eau, débit, concentration de sédiments et d’autres paramètres physico-chimiques de qualité de l’eau). De nos jours, la mise au point des techniques par satellite, tels que l’altimétrie, permet de compléter efficacement les données obtenues par les réseaux de mesure dans les rivières. L’intensification des événements extrêmes dans la région amazonienne (inondations, sécheresses), liés au changement climatique actuel associé á un changement rapide de l’utilisation des terres (déforestation, pratiques agricoles), ont un impact direct sur la production de sédiments. Connaître les flux de matières transportés par les rivières est essentiel pour la navigation fluviale ainsi que pour guider l’exploitation des ressources naturelles (eau, pétrole, minéraux), connaître le trajet de particules polluantes ou concevoir des infrastructures.The Amazon basin is the largest basin in the world. The implementation of the HYBAM observatory, which has installed a large network of hydrological stations located from the Andean foothills down to the Atlantic Ocean, allows since 2003, generate periodical and reliable records (such as water level, flow, sediment concentration and other physicochemical parameters of water quality) along the entire basin. Nowadays, the development of satellite techniques, (such as altimetry), makes it possible to efficiently complete the data obtained by the measurement networks in rivers. The intensification of extreme events in the Amazon region (floods, droughts) occurred as a result of the current Climate Change associated with a more rapid change of land use (deforestation, agricultural practices) have a direct impact on sediment production. It is essential to know the flows of the materials transported by rivers, both for river navigation as well as to guide the exploitation of natural resources (water, oil, minerals), to know the transport of particulate pollutants, or to design infrastructures

Programmable antivirals targeting critical conserved viral RNA secondary structures from influenza A virus and SARS-CoV-2

Influenza A virus’s (IAV’s) frequent genetic changes challenge vaccine strategies and engender resistance to current drugs. We sought to identify conserved and essential RNA secondary structures within IAV’s genome that are predicted to have greater constraints on mutation in response to therapeutic targeting. We identified and genetically validated an RNA structure (packaging stem–loop 2 (PSL2)) that mediates in vitro packaging and in vivo disease and is conserved across all known IAV isolates. A PSL2-targeting locked nucleic acid (LNA), administered 3 d after, or 14 d before, a lethal IAV inoculum provided 100% survival in mice, led to the development of strong immunity to rechallenge with a tenfold lethal inoculum, evaded attempts to select for resistance and retained full potency against neuraminidase inhibitor-resistant virus. Use of an analogous approach to target SARS-CoV-2, prophylactic administration of LNAs specific for highly conserved RNA structures in the viral genome, protected hamsters from efficient transmission of the SARS-CoV-2 USA_WA1/2020 variant. These findings highlight the potential applicability of this approach to any virus of interest via a process we term ‘programmable antivirals’, with implications for antiviral prophylaxis and post-exposure therapy