Microsatellite in Aeschynomene falcata (Leguminosae): Diversity, cross-amplification, and chromosome localization

Aeschynomene falcata is an important forage species; however, because of low seed production, it is underutilized as forage species. Aeschynomene is a polyphyletic genus with a challenging taxonomic position. Two subgenera have been proposed, and it is suggested that Aeschynomene can be split in 2 genera. Thus, new markers, such as microsatellite sequences, are desirable for improving breeding programs for A. falcata. Based on transferability and in situ localization, these microsatellite sequences can be applied as chromosome markers in the genus Aeschynomene and closely related genera. Here, we report the first microsatellite library developed for this genus; 11 microsatellites were characterized, with observed and expected heterozygosities ranging from 0.0000 to 0.7143 and from 0.1287 to 0.8360, respectively. Polymorphic information content varied from 0.1167 to 0.7786. The departure from Hardy-Weinberg equilibrium may have resulted from frequent autogamy, which is characteristic of A. falcata. Of the 11 microsatellites, 9 loci were cross-amplified in A. brevipes and A. paniculata and 7 in Dalbergia nigra and Machaerium vestitum. Five of these 7 cross-amplified microsatellites were applied as probes during the in situ hybridization assay and 2 showed clear signals on A. falcata chromosomes, ensuring their viability as chromosome markers1341039010397CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP471708/2010-02011/22215-3; 2010/19132-

Microsatellite In Aeschynomene Falcata (leguminosae): Diversity, Cross-amplification, And Chromosome Localization.

Aeschynomene falcata is an important forage species; however, because of low seed production, it is underutilized as forage species. Aeschynomene is a polyphyletic genus with a challenging taxonomic position. Two subgenera have been proposed, and it is suggested that Aeschynomene can be split in 2 genera. Thus, new markers, such as microsatellite sequences, are desirable for improving breeding programs for A. falcata. Based on transferability and in situ localization, these microsatellite sequences can be applied as chromosome markers in the genus Aeschynomene and closely related genera. Here, we report the first microsatellite library developed for this genus; 11 microsatellites were characterized, with observed and expected heterozygosities ranging from 0.0000 to 0.7143 and from 0.1287 to 0.8360, respectively. Polymorphic information content varied from 0.1167 to 0.7786. The departure from Hardy-Weinberg equilibrium may have resulted from frequent autogamy, which is characteristic of A. falcata. Of the 11 microsatellites, 9 loci were cross-amplified in A. brevipes and A. paniculata and 7 in Dalbergia nigra and Machaerium vestitum. Five of these 7 cross-amplified microsatellites were applied as probes during the in situ hybridization assay and 2 showed clear signals on A. falcata chromosomes, ensuring their viability as chromosome markers.1310390-

Effects of molybdenum, nickel, and nitrogen sources on the mineral nutrition and growth of rice plants.

The aims of the current work were to measure the effect of Mo and Ni on dry-matter yield of rice plants supplied with two N sources (ammonium nitrate and urea) and to evaluate the influence of Mo and Ni on variables related to dry-matter yield, such as activity of both urease and nitrate reductase, chlorophyll, net photosynthesis rate, total N, nitrate content, and Mo and Ni concentrations in roots and shoots

Stellar equilibrium configurations of white dwarfs in the f(R,T)f(R,T) gravity

In this work we investigate the equilibrium configurations of white dwarfs in a modified gravity theory, na\-mely, $f(R,T)$ gravity, for which $R$ and $T$ stand for the Ricci scalar and trace of the energy-momentum tensor, respectively. Considering the functional form $f(R,T)=R+2\lambda T$, with $\lambda$ being a constant, we obtain the hydrostatic equilibrium equation for the theory. Some physical properties of white dwarfs, such as: mass, radius, pressure and energy density, as well as their dependence on the parameter $\lambda$ are derived. More massive and larger white dwarfs are found for negative values of $\lambda$ when it decreases. The equilibrium configurations predict a maximum mass limit for white dwarfs slightly above the Chandrasekhar limit, with larger radii and lower central densities when compared to standard gravity outcomes. The most important effect of $f(R,T)$ theory for massive white dwarfs is the increase of the radius in comparison with GR and also $f(R)$ results. By comparing our results with some observational data of massive white dwarfs we also find a lower limit for $\lambda$, namely, $\lambda >- 3\times 10^{-4}$.Comment: To be published in EPJ

Performance reprodutiva de cabras Alpinas e Saanen tratadas com gonadotrofina coriônica humana no quinto e vigésimo dia pós-serviço durante a estação de monta induzida.

Resumo: Objetivou-se com este estudo avaliar o efeito da administração da gonadotrofina coriônica humana (hCG) no quinto e vigésimo dia do ciclo estral sobre a taxa de gestação de cabras da raça Alpina e Saanen, durante a estação de acasalamento induzida por fotoperíodo artificial. Foram utilizados 151 animais, sendo 85 da raça Alpina e 66 da Saanen. Do total, 110 foram submetidas à monta natural e 41 à inseminação artificial. Após a identificação do estro e acasalamento ou inseminação, as fêmeas foram divididas ao acaso, em dois tratamentos:T1 = 1mL de solução salina intramuscular no quinto e vigésimo dia após a cobertura e T2 = 250 UI de hCG no mesmo esquema. A gestação foi verificada por ultra-sonografia trans-retal aos 35 dias após a cobertura. Não houve efeito da administração do hCG sobre a taxa de gestação, taxa de parição e número de fetos nascidos (P>0,05). As diferentes raças empregadas neste estudo também não influenciaram os parâmetros estudados. Independentemente da aplicação do hCG, houve decréscimo significativo na taxa de gestação quando se empregou a inseminação artificial (P0,05) of the hCG administration on gestation rate, parturition rate and number of born foetus. The difference breeds used in this study didn?t influence the studied parameters. Independently of the application of hCG, there was significant decrease in the gestation rate when the artificial insemination was used (P <0,05). Under the conditions of this study, the hCG administration in the fifth and twentieth day of the estrous cycle estral doesn?t present any effect on gestation rate of goats of the Alpine and Saanen breeds

Simultaneous Optical Model Analyses of Elastic Scattering, Breakup, and Fusion Cross Section Data for the 6^{6}He + 209^{209}Bi System at Near-Coulomb-Barrier Energies

Based on an approach recently proposed by us, simultaneous $\chi^{2}$-analyses are performed for elastic scattering, direct reaction (DR) and fusion cross sections data for the $^{6}$He+$^{209}$Bi system at near-Coulomb-barrier energies to determine the parameters of the polarization potential consisting of DR and fusion parts. We show that the data are well reproduced by the resultant potential, which also satisfies the proper dispersion relation. A discussion is given of the nature of the threshold anomaly seen in the potential

Search for the f(R,T)f(R,T) gravity functional form via gaussian processes

The $f(R,T)$ gravity models, for which $R$ is the Ricci scalar and $T$ is the trace of the energy-momentum tensor, elevate the degrees of freedom of the renowned $f(R)$ theories, by making the Einstein field equations of the theory to also depend on $T$. While such a dependence can be motivated by quantum effects, the existence of imperfect or extra fluids, or even a cosmological ``constant'' which effectively depends on $T$, the formalism can truly surpass some deficiencies of $f(R)$ gravity. As the $f(R,T)$ function is arbitrary, several parametric models have been proposed {\it ad hoc} in the literature and posteriorly confronted with observational data. In the present article, we use gaussian process to construct an $f(R,T)=R+f(T)$ model. To apply the gaussian process we use a series of measurements of the Hubble parameter. We then analytically obtain the functional form of the function. By construction, this form, which is novel in the literature, is well-adjusted to cosmological data. In addition, by extrapolating our reconstruction to redshift $z=0$, we were able to constrain the Hubble constant value to $H_0=69.97\pm4.13$$\rm \ km \ s^{-1} \ Mpc^{-1}$with$5\%$ precision. Lastly, we encourage the application of the functional form herewith obtained to other current problems of observational cosmology and astrophysics, such as the rotation curves of galaxies