Augite in Komatiitic Basalts: A Study of the Mobility of Trace Elements During Rock Alteration

Komatiitic basalts are ultramafic rocks formed from volcanic liquids containing less than 18 wt.% magnesium oxide that can be linked, spatially, geochemically and texturally to komatiites. Archean komatiites and komatiitic basalts are critical to models of early Earth’s mantle evolution. Major and trace elements in these rocks are poorly preserved due to early sub-seafloor alteration with superimposed regional metamorphism: in particular the alkali, alkaline earths, and other elements with low valence numbers. Current analytical methods focus on analyzing whole rock, fresh olivines and melt inclusions to evaluate elemental concentrations and for petrogenetic studies. Although these methods establish elemental concentrations, REE, Sr, Na and Ba should be better represented by evaluating trace elements in fresh augites from komatiitic basalts, which contain moderate levels of trace element concentrations that olivines lack. In this study trace elements were analyzed within samples from two different formations (Mendon and Weltevreden) within the Barberton Greenstone Belt, South Africa. These samples contain fresh augites from komatiitic basalts and were analyzed using LA-ICP-MS. Results suggest that REE in augites from the freshest komatiitic basalts closely match the predicted values calculated from bulk rock analyses when distribution coefficients and Rayleigh fractionation are taken into account. Zr and Ti, exhibit anomalies within the augites that correspond to anomalies found in bulk rock data. This supports the contention that both the REE and HFSE remain a closed system within these rocks. However, Na, Sr and Ba were mobilized within the bulk rock during alteration. Values for Na are particularly difficult to establish from bulk rock data. Even when they are nearly constant within a suite of flows, their values are far from those predicted from augite analyses. Sr has negative anomalies in these rocks, and this is confirmed to be a trait acquired from the magmas through augite analyses. These data confirm two important components of komatiite petrogenesis: 1) fractionation of a high pressure phase to modify the HFSE, and 2) assimilation of felsic material during crustal ascent. Important radiogenic systems, such as Rb-Sr, Nd-Sm, and Lu-Hf, should be evaluated using augite proxies from single mineral SIMS, LA-ICP_MS, or mineral separations

Transient increases in intracellular calcium and reactive oxygen species levels in TCam-2 cells exposed to microgravity

The effects of microgravity on functions of the human body are well described, including alterations in the male and female reproductive systems. In the present study, TCam-2 cells, which are considered a good model of mitotically active male germ cells, were used to investigate intracellular signalling and cell metabolism during exposure to simulated microgravity, a condition that affects cell shape and cytoskeletal architecture. After a 24 hour exposure to simulated microgravity, TCam-2 cells showed 1) a decreased proliferation rate and a delay in cell cycle progression, 2) increased anaerobic metabolism accompanied by increased levels of intracellular Ca(2+), reactive oxygen species and superoxide anion and modifications in mitochondrial morphology. Interestingly, all these events were transient and were no longer evident after 48 hours of exposure. The presence of antioxidants prevented not only the effects described above but also the modifications in cytoskeletal architecture and the activation of the autophagy process induced by simulated microgravity. In conclusion, in the TCam-2 cell model, simulated microgravity activated the oxidative machinery, triggering transient macroscopic cell events, such as a reduction in the proliferation rate, changes in cytoskeleton-driven shape and autophagy activation

An investigation on skeleton-based top-down modelling approaches of complex industrial product

In industry, today's approach to assembly design is still largely based on a bottom-up approach which, in contrast with the most advanced top-down techniques, is unfit to deal with very large and complex products. The reason for this lies in the high number of relationships to be established between parts and in the lack of a high-level control of the assembly design. This makes the management of design changes a labor-intensive process and the capture of design intent difficult to achieve. The paper, referring to the most advanced research fields of Concurrent Engineering and Knowledge-Based Engineering, focuses on a top-down modelling approach based on skeleton, which constitutes the most natural but still scarcely exploited way to attain a high reactivity to design modifications. Through the application of suitable methodologies, such as that one for a SKeLeton geometry-based Assembly Context Definition (SKL-ACD), the skeleton is also able to capture and codify assembly process engineering information since the early phases of the product development process. With the purpose of promoting the knowledge of these skeleton-based modelling techniques, that have a great relevance for training professional, technical and mechanical engineers, this paper implements the SKL-ACD methodology to an industrial case study in order to identify, with a unique and repeatable workflow, the reference geometrical entities and the mutual relationships to embed into the product skeleton. The skeleton types and the related fields of use are also described, placing particular emphasis on problems or shortcomings still not resolved, especially in consideration of the need to assist the designer in defining the impact of a parameter on assembly modification and in avoiding loops while defining formulas. A new tool, in the form of a multilayer graph, is finally proposed that is able to display and differentiate clearly the formulas, the design parameters and the impact of their modification on skeleton entities and members of the assembly

Altered Kv2.1 functioning promotes increased excitability in hippocampal neurons of an Alzheimer's disease mouse model.

Altered neuronal excitability is emerging as an important feature in Alzheimer's disease (AD). Kv2.1 potassium channels are important modulators of neuronal excitability and synaptic activity. We investigated Kv2.1 currents and its relation to the intrinsic synaptic activity of hippocampal neurons from 3xTg-AD (triple transgenic mouse model of Alzheimer's disease) mice, a widely employed preclinical AD model. Synaptic activity was also investigated by analyzing spontaneous [Ca(2+)]i spikes. Compared with wild-type (Non-Tg (non-transgenic mouse model)) cultures, 3xTg-AD neurons showed enhanced spike frequency and decreased intensity. Compared with Non-Tg cultures, 3xTg-AD hippocampal neurons revealed reduced Kv2.1-dependent Ik current densities as well as normalized conductances. 3xTg-AD cultures also exhibited an overall decrease in the number of functional Kv2.1 channels. Immunofluorescence assay revealed an increase in Kv2.1 channel oligomerization, a condition associated with blockade of channel function. In Non-Tg neurons, pharmacological blockade of Kv2.1 channels reproduced the altered pattern found in the 3xTg-AD cultures. Moreover, compared with untreated sister cultures, pharmacological inhibition of Kv2.1 in 3xTg-AD neurons did not produce any significant modification in Ik current densities. Reactive oxygen species (ROS) promote Kv2.1 oligomerization, thereby acting as negative modulator of the channel activity. Glutamate receptor activation produced higher ROS levels in hippocampal 3xTg-AD cultures compared with Non-Tg neurons. Antioxidant treatment with N-Acetyl-Cysteine was found to rescue Kv2.1-dependent currents and decreased spontaneous hyperexcitability in 3xTg-AD neurons. Analogous results regarding spontaneous synaptic activity were observed in neuronal cultures treated with the antioxidant 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox). Our study indicates that AD-related mutations may promote enhanced ROS generation, oxidative-dependent oligomerization, and loss of function of Kv2.1 channels. These processes can be part on the increased neuronal excitability of these neurons. These steps may set a deleterious vicious circle that eventually helps to promote excitotoxic damage found in the AD brain

What parents say about children’s inequality of opportunities : a study in Mauritius

Longitudinal studies showing the beneficial impact of early childhood education on later academic achievement have contributed to persuading policy-makers and academia that early childhood is the best time to address inequalities. However, the voice of parents is often absent from these debates. We investigated the perspectives of parents on children's inequalities of opportunities and on the role of education and early childhood care in equalising life chances. The study specifically explored the views of 26 parents in the sub-Saharan African country of Mauritius through focus group discussions. Findings of the study suggested that parents tend to adhere to the discourse of parental responsibility as a key factor in children's inequalities. Yet they also showed that parents have potential to criticise and deconstruct this narrative, as they experience structural circumstances, such as poverty or discrimination, shaping their life opportunities and those of their children

Characterization of an emergent clone of enteroinvasive Escherichia coli circulating in Europe

Enteroinvasive Escherichia coli (EIEC) cause intestinal illness indistinguishable from that caused by Shigella, mainly in developing countries. Recently an upsurge of cases of EIEC infections has been observed in Europe, with two large outbreaks occurring in Italy and in the United Kingdom. We have characterized phenotypically and genotypically the strains responsible for these epidemics together with an additional isolate from a sporadic case isolated in Spain. The three isolates belonged to the same rare serotype O96:H19 and were of sequence type ST-99, never reported before in EIEC or Shigella. The EIEC strains investigated possessed all the virulence genes harboured on the large plasmid conferring the invasive phenotype to EIEC and Shigella while showing only some of the known chromosomal virulence genes and none of the described pathoadaptative mutations. At the same time, they displayed motility abilities and biochemical requirements resembling more closely those of the non-pathogenic E. coli rather than the EIEC and Shigella strains used as reference. Our observations suggested that the O96:H19 strains belong to an emerging EIEC clone, which could be the result of a recent event of acquisition of the invasion plasmid by commensal E. coli

Current surgical concepts and indications in the management of the short bowel state: A call for the use of multidisciplinary intestinal rehabilitation programs

The mainstay of management for short bowel syndrome (SBS) is to promote access to the best quality of care provided by the intestinal rehabilitation program (IRP) in specialized centres. When treating SBS patients, the main goal is to minimize disease-associated complications, as well as achieve enteral autonomy. Surgical strategies should be selected cautiously upon the actual state of the bowel with respect to what it is clinically relevant for that specific patient. To this aim, a personalized and multidisciplinary approach for such a complex syndrome is needed