Checkpoint proteins come under scrutiny

Details are emerging of the interactions between the kinetochore and various spindle checkpoint proteins that ensure that sister chromatids are equally divided between daughter cells during cell division

Microbial manganese and sulfate reduction in Black Sea shelf sediments

The microbial ecology of anaerobic carbon oxidation processes was investigated in Black Sea shelf sediments from mid-shelf with well-oxygenated bottom water to the oxic-anoxic chemocline at the shelf-break. At all stations, organic carbon (Corg) oxidation rates were rapidly attenuated with depth in anoxically incubated sediment. Dissimilatory Mn reduction was the most important terminal electron-accepting process in the active surface layer to a depth of ∼1 cm, while SO42− reduction accounted for the entire Corg oxidation below. Manganese reduction was supported by moderately high Mn oxide concentrations. A contribution from microbial Fe reduction could not be discerned, and the process was not stimulated by addition of ferrihydrite. Manganese reduction resulted in carbonate precipitation, which complicated the quantification of Corg oxidation rates. The relative contribution of Mn reduction to Corg oxidation in the anaerobic incubations was 25 to 73% at the stations with oxic bottom water. In situ, where Mn reduction must compete with oxygen respiration, the contribution of the process will vary in response to fluctuations in bottom water oxygen concentrations. Total bacterial numbers as well as the detection frequency of bacteria with fluorescent in situ hybridization scaled to the mineralization rates. Most-probable-number enumerations yielded up to 105 cells of acetate-oxidizing Mn-reducing bacteria (MnRB) cm−3, while counts of Fe reducers were <102 cm−3. At two stations, organisms affiliated with Arcobacter were the only types identified from 16S rRNA clone libraries from the highest positive MPN dilutions for MnRB. At the third station, a clone type affiliated with Pelobacter was also observed. Our results delineate a niche for dissimilatory Mn-reducing bacteria in sediments with Mn oxide concentrations greater than ∼10 μmol cm−3 and indicate that bacteria that are specialized in Mn reduction, rather than known Mn and Fe reducers, are important in this niche

Searching for signatures of planet formation in stars with circumstellar debris discs

(Abridged) Tentative correlations between the presence of dusty debris discs and low-mass planets have been presented. In parallel, detailed chemical abundance studies have reported different trends between samples of planet and non-planet hosts. We determine in a homogeneous way the metallicity, and abundances of a sample of 251 stars including stars with known debris discs, with debris discs and planets, and only with planets. Stars with debris discs and planets have the same [Fe/H] behaviour as stars hosting planets, and they also show a similar -Tc trend. Different behaviour in the -Tc trend is found between the samples of stars without planets and the samples of planet hosts. In particular, when considering only refractory elements, negative slopes are shown in cool giant planet hosts, whilst positive ones are shown in stars hosting low-mass planets. Stars hosting exclusively close-in giant planets show higher metallicities and positive -Tc slope. A search for correlations between the -Tc slopes and the stellar properties reveals a moderate but significant correlation with the stellar radius and as well as a weak correlation with the stellar age. The fact that stars with debris discs and stars with low-mass planets do not show neither metal enhancement nor a different -Tc trend might indicate a correlation between the presence of debris discs and the presence of low-mass planets. We extend results from previous works which reported differences in the -Tc trends between planet hosts and non hosts. However, these differences tend to be present only when the star hosts a cool distant planet and not in stars hosting exclusively low-mass planets.Comment: Accepted for publication in Astronomy and Astrophysic

Atypical protein kinase C (PKCzeta/lambda) is a convergent downstream target of the insulin-stimulated phosphatidylinositol 3-kinase and TC10 signaling pathways.

Insulin stimulation of adipocytes resulted in the recruitment of atypical PKC (PKCzeta/lambda) to plasma membrane lipid raft microdomains. This redistribution of PKCzeta/lambda was prevented by Clostridium difficile toxin B and by cholesterol depletion, but was unaffected by inhibition of phosphatidylinositol (PI) 3-kinase activity. Expression of the constitutively active GTP-bound form of TC10 (TC10Q/75L), but not the inactive GDP-bound mutant (TC10/T31N), targeted PKCzeta/lambda to the plasma membrane through an indirect association with the Par6-Par3 protein complex. In parallel, insulin stimulation as well as TC10/Q75L resulted in the activation loop phosphorylation of PKCzeta. Although PI 3-kinase activation also resulted in PKCzeta/lambda phosphorylation, it was not recruited to the plasma membrane. Furthermore, insulin-induced GSK-3beta phosphorylation was mediated by both PI 3-kinase-PKB and the TC10-Par6-atypical PKC signaling pathways. Together, these data demonstrate that PKCzeta/lambda can serve as a convergent downstream target for both the PI 3-kinase and TC10 signaling pathways, but only the TC10 pathway induces a spatially restricted targeting to the plasma membrane

³¹P Saturation Transfer and Phosphocreatine Imaging in the Monkey Brain

³¹P magnetic resonance imaging with chemical-shift discrimination by selective excitation has been employed to determine the phosphocreatine (PCr) distribution in the brains of three juvenile macaque monkeys. PCr images were also obtained while saturating the resonance of the {gamma}-phosphate of ATP, which allowed the investigation of the chemical exchange between PCr and the {gamma}-phosphate of ATP catalyzed by creatine kinase. Superposition of the PCr images over the proton image of the same monkey brain revealed topological variations in the distribution of PCr and creatine kinase activity. PCr images were also obtained with and without visual stimulation. In two out of four experiments, an apparently localized decrease in PCr concentration was noted in visual cortex upon visual stimulation. This result is interpreted in terms of a possible role for the local ADP concentration in stimulating the accompanying metabolic response

Gauged WZW models for space-time groups and gravitational actions

In this paper we investigate gauged Wess-Zumino-Witten models for space-time groups as gravitational theories, following the trend of recent work by Anabalon, Willison and Zanelli. We discuss the field equations in any dimension and study in detail the simplest case of two space-time dimensions and gauge group SO(2,1). For this model we study black hole solutions and we calculate their mass and entropy which resulted in a null value for both.Comment: 26 pages, no figure