Agroecologia para Escola Guarany Karaí Kuerí Renda

A presente pesquisa relaciona ou problemas socioambientais na aldeia Sapucaí, através da escola indígena Karaí Kuery Renda tomando em considerações as construções etnoagroecológicas. Aplicou-se como metodologia observação de campo, registros com fotos e desenhos elaborados por crianças. Como objetivos empíricos de longo alcance, visou-se a integração das famílias dos estudantes na comunidade escolar. A prática realizada na escola registrou a concepção dos alunos sobre sua aldeia, visando fundamentar construções de documentos pedagógicos com sentido agroecológico. A partir deste trabalho podem ser formados multiplicadores agroecológicos, de forma a favorecer o desenvolvimento cultural, político, econômico e ambiental. O levantamento de práticas já existentes pela comunidade, somado ás novas propostas criadas no que condiz a agroecologia, pode vir a ser importante para aliar a proposta de junção do conhecimento tradicional da comunidade referida, com projetos pedagógicos e ambientais.Eje A5: Sistemas de conocimientoFacultad de Ciencias Agrarias y Forestale

Agroecologia para Escola Guarany Karaí Kuerí Renda

A presente pesquisa relaciona ou problemas socioambientais na aldeia Sapucaí, através da escola indígena Karaí Kuery Renda tomando em considerações as construções etnoagroecológicas. Aplicou-se como metodologia observação de campo, registros com fotos e desenhos elaborados por crianças. Como objetivos empíricos de longo alcance, visou-se a integração das famílias dos estudantes na comunidade escolar. A prática realizada na escola registrou a concepção dos alunos sobre sua aldeia, visando fundamentar construções de documentos pedagógicos com sentido agroecológico. A partir deste trabalho podem ser formados multiplicadores agroecológicos, de forma a favorecer o desenvolvimento cultural, político, econômico e ambiental. O levantamento de práticas já existentes pela comunidade, somado ás novas propostas criadas no que condiz a agroecologia, pode vir a ser importante para aliar a proposta de junção do conhecimento tradicional da comunidade referida, com projetos pedagógicos e ambientais.Eje A5: Sistemas de conocimientoFacultad de Ciencias Agrarias y Forestale

Agroecologia para Escola Guarany Karaí Kuerí Renda

A presente pesquisa relaciona ou problemas socioambientais na aldeia Sapucaí, através da escola indígena Karaí Kuery Renda tomando em considerações as construções etnoagroecológicas. Aplicou-se como metodologia observação de campo, registros com fotos e desenhos elaborados por crianças. Como objetivos empíricos de longo alcance, visou-se a integração das famílias dos estudantes na comunidade escolar. A prática realizada na escola registrou a concepção dos alunos sobre sua aldeia, visando fundamentar construções de documentos pedagógicos com sentido agroecológico. A partir deste trabalho podem ser formados multiplicadores agroecológicos, de forma a favorecer o desenvolvimento cultural, político, econômico e ambiental. O levantamento de práticas já existentes pela comunidade, somado ás novas propostas criadas no que condiz a agroecologia, pode vir a ser importante para aliar a proposta de junção do conhecimento tradicional da comunidade referida, com projetos pedagógicos e ambientais.Eje A5: Sistemas de conocimientoFacultad de Ciencias Agrarias y Forestale

Actions of Crotalus durissus terrificus venom and crotoxin on the isolated rat kidney

Many studies have reported the occurrence of lethal acute renal failure after snakebites. The aim of the present investigation was to determine alterations in renal function produced by Crotalus durissus terrificus venom and crotoxin as well as the histological alterations induced by these venoms. Isolated kidneys from Wistar rats weighing 240 to 280 g were perfused with Krebs-Henseleit solution containing 6 g% of previously dialyzed bovine serum albumin. The effects of Crotalus durissus terrificus venom and crotoxin were studied on glomerular filtration rate (GFR), urinary flow (UF), perfusion pressure (PP) and percentage sodium tubular transport (%TNa+). The infusion of Crotalus durissus terrificus venom (10 µg/ml) and crotoxin (10 µg/ml) increased GFR (control80 = 0.78 ± 0.07, venom80 = 1.1 ± 0.07, crotoxin80 = 2.0 ± 0.05 ml g-1 min-1, P<0.05) and UF (control80 = 0.20 ± 0.02, venom80 = 0.32 ± 0.03, crotoxin80 = 0.70 ± 0.05 ml g-1 min-1, P<0.05), and decreased %TNa+ (control100 = 75.0 ± 2.3, venom100 = 62.9 ± 1.0, crotoxin80 = 69.0 ± 1.0 ml g-1 min-1, P<0.05). The infusion of crude venom tended to reduce PP, although the effect was not significant, whereas with crotoxin PP remained stable during the 100 min of perfusion. The kidneys perfused with crude venom and crotoxin showed abundant protein material in the urinary space and tubules. We conclude that Crotalus durissus terrificus venom and crotoxin, its major component, cause acute nephrotoxicity in the isolated rat kidney. The current experiments demonstrate a direct effect of venom and crotoxin on the perfused isolated kidney

Renal Effects Of Supernatant From Macrophages Activated By Crotalus Durissus Cascavella Venom: The Role Of Phospholipase A2 And Cyclooxygenase

In Brazil, the genus Crotalus is responsible for approximately 1500 cases of snakebite annually. The most common complication in the lethal cases is acute renal failure, although the mechanisms of the damaging effects are not totally understood. In this work, we have examined the renal effects caused by a supernatant of macrophages stimulated by Crotalus durissus cascavella venom as well as the potential role of phospholipase A2 and cyclo-oxygenase. Rat peritoneal macrophages were collected and placed in a RPMI medium and stimulated by crude Crotalus durissus cascavella venom (1, 3 or 10 μg/ml) for 1 hr. They were then washed and kept in a culture for 2 hr. The supernatant (1 ml) was tested in an isolated perfused rat kidney. The first 30 min. of each experiment were used as an internal control, and the supernatant was added to the system after this period. All experiments lasted 120 min. A study of toxic effect on perfusion pressure, glomerular filtration rate, urinary flow, percent of sodium tubular transport and percent of proximal tubular sodium transport was made. The lowest concentration of venom (1 μg/ml) was not statistically different from the control values. The most intense effects were seen at 10 μg/ml for all renal parameters. The infusion of the supernatant of macrophages stimulated with crude venom (3 or 10 μg/ml) increased the perfusion pressure, glomerular filtration rate and urinary flow, decreased the percent of sodium tubular transport and percent of proximal tubular sodium transport. Dexamethasone (10 μM) and quinacrine (10 μM) provided protection against the effect of the venom on glomerular filtration rate, urinary flow, percent of sodium tubular transport, percent of proximal tubular sodium transport and perfusion pressure. 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