1,145 research outputs found
Diurnal variation and size dependence of the hygroscopicity of organic aerosol at a forest site in Wakayama, Japan: their relationship to CCN concentrations
Formation of biogenic secondary organic aerosol (BSOA)
and its subsequent evolution can modify the hygroscopicity of the organic
aerosol component (OA) in the forest atmosphere, and affect the
concentrations of cloud condensation nuclei (CCN) there. In this study,
size-resolved aerosol hygroscopic growth at 85 % relative humidity and
size-resolved aerosol composition were measured using a hygroscopic tandem
differential mobility analyzer and an aerosol mass spectrometer,
respectively, at a forest site in Wakayama, Japan, in August and September
2015. The hygroscopicity parameter of OA (κorg) presented daily
minima in the afternoon hours, and it also showed an increase with the increase in particle dry diameter. The magnitudes of the diurnal variations in
κorg for particles with dry diameters of 100 and 300 nm were on
average 0.091 and 0.096, respectively, and the difference in κorg between particles with dry diameters of 100 and 300 nm was on
average 0.056. The relative contributions of the estimated fresh BSOA and
regional OA to total OA could explain 40 % of the observed diurnal
variations and size dependence of κorg. The hygroscopicity
parameter of fresh BSOA was estimated to range from 0.089 to 0.12 for
particles with dry diameters from 100 to 300 nm. Compared with the use of
time- and size-resolved κorg, the use of time- and
size-averaged κorg leads to under- and over-estimation of the
fractional contribution of OA to CCN number concentrations in the range from
−5.0 % to 26 %. This indicates that the diurnal variations and
size dependence of κorg strongly affect the overall
contribution of OA to CCN concentrations. The fractional contribution of
fresh BSOA to CCN number concentrations could reach 0.28 during the period
of intensive BSOA formation. The aging of the fresh BSOA, if it occurs,
increases the estimated contribution of BSOA to CCN number concentrations by
52 %–84 %.</p
Exhaustion of nucleus pulposus progenitor cells with ageing and degeneration of the intervertebral disc
published_or_final_versio
Comprehensive Functional Analyses of Expressed Sequence Tags in Common Wheat (Triticum aestivum)
About 1 million expressed sequence tag (EST) sequences comprising 125.3 Mb nucleotides were accreted from 51 cDNA libraries constructed from a variety of tissues and organs under a range of conditions, including abiotic stresses and pathogen challenges in common wheat (Triticum aestivum). Expressed sequence tags were assembled with stringent parameters after processing with inbuild scripts, resulting in 37 138 contigs and 215 199 singlets. In the assembled sequences, 10.6% presented no matches with existing sequences in public databases. Functional characterization of wheat unigenes by gene ontology annotation, mining transcription factors, full-length cDNA, and miRNA targeting sites were carried out. A bioinformatics strategy was developed to discover single-nucleotide polymorphisms (SNPs) within our large EST resource and reported the SNPs between and within (homoeologous) cultivars. Digital gene expression was performed to find the tissue-specific gene expression, and correspondence analysis was executed to identify common and specific gene expression by selecting four biotic stress-related libraries. The assembly and associated information cater a framework for future investigation in functional genomics
Transcriptome analysis of thermogenic Arum concinnatum reveals the molecular components of floral scent production.
Several plant species can generate enough heat to increase their internal floral temperature above ambient temperature. Among thermogenic plants, Arum concinnatum shows the highest respiration activity during thermogenesis. However, an overall understanding of the genes related to plant thermogenesis has not yet been achieved. In this study, we performed de novo transcriptome analysis of flower organs in A. concinnatum. The de novo transcriptome assembly represented, in total, 158,490 non-redundant transcripts, and 53,315 of those showed significant homology with known genes. To explore genes associated with thermogenesis, we filtered 1266 transcripts that showed a significant correlation between expression pattern and the temperature trend of each sample. We confirmed five putative alternative oxidase transcripts were included in filtered transcripts as expected. An enrichment analysis of the Gene Ontology terms for the filtered transcripts suggested over-representation of genes involved in 1-deoxy-d-xylulose-5-phosphate synthase (DXS) activity. The expression profiles of DXS transcripts in the methyl-d-erythritol 4-phosphate (MEP) pathway were significantly correlated with thermogenic levels. Our results suggest that the MEP pathway is the main biosynthesis route for producing scent monoterpenes. To our knowledge, this is the first report describing the candidate pathway and the key enzyme for floral scent production in thermogenic plants.Yoshihiko Onda, Keiichi Mochida, Takuhiro Yoshida, Tetsuya Sakurai, Roger S. Seymour, Yui Umekawa, Stergios Arg Pirintsos, Kazuo Shinozaki, Kikukatsu It
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Aerosol particles collected on aircraft flights over the northwestern Pacific region during the ACE-Asia campaign: Composition and major sources of the organic compounds
Atmospheric particulate matter, collected over the polluted east Asia/Pacific region in spring 2001 during research flights with the National Center for Atmospheric Research (NCAR) C-130 aircraft, was analyzed for different types of organic compounds using capillary gas chromatography–mass spectrometry. More than 70 organic species were detected in the aerosols and grouped into different compound classes on the basis of functional groups, including n-alkanes, polycyclic aromatic hydrocarbons, fatty acids, dehydroabietic acid, alkanols, water-soluble sugars (including glucose, sucrose, mycose, and levoglucosan), monocarboxylic and dicarboxylic acids, urea, and phthalates. Interestingly, the water-soluble compounds (72–133 ng m–3) were found to account for 16–50% (average 34%) of the total identified compound mass (TCM). Organic compounds were further categorized into several groups to suggest their sources. Fossil fuel combustion was recognized as the most significant source for the TCM (contributing 33–80% of TCM, average 50%), followed by soil resuspension (5–25%, average 19%) and secondary oxidation products (4–15%, average 9%). In contrast, the contribution of natural sources such as terrestrial plant wax and marine lipids (fatty acids and alkanols) was relatively small (3.4% and 9.4% on average, respectively). Biomass burning was suggested to contribute only a minor portion to the TCM of the Asian aerosols during the spring season (1.4% on average based on levoglucosan). However, levoglucosan may have been hydrolyzed and/or oxidized in part during long-range transport, and therefore this value represents a lower limit. The organic compound compositions of these samples are very different from those reported for aerosol particles of the Atlantic Ocean and from
the earlier data for the mid-Pacific in terms of the abundant presence of water-soluble compounds consisting of saccharides, anhydrosaccharides, and the secondary dicarboxylic acids. This study demonstrated that the organic tracer approach can be carried out on small samples acquired on aircraft and is useful to better understand the sources of organic aerosols over the Asia/Pacific region
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