Gene Expression and Functional Studies of the Optic Nerve Head Astrocyte Transcriptome from Normal African Americans and Caucasian Americans Donors

To determine whether optic nerve head (ONH) astrocytes, a key cellular component of glaucomatous neuropathy, exhibit differential gene expression in primary cultures of astrocytes from normal African American (AA) donors compared to astrocytes from normal Caucasian American (CA) donors.We used oligonucleotide Affymetrix microarray (HG U133A & HG U133A 2.0 chips) to compare gene expression levels in cultured ONH astrocytes from twelve CA and twelve AA normal age matched donor eyes. Chips were normalized with Robust Microarray Analysis (RMA) in R using Bioconductor. Significant differential gene expression levels were detected using mixed effects modeling and Statistical Analysis of Microarray (SAM). Functional analysis and Gene Ontology were used to classify differentially expressed genes. Differential gene expression was validated by quantitative real time RT-PCR. Protein levels were detected by Western blots and ELISA. Cell adhesion and migration assays tested physiological responses. Glutathione (GSH) assay detected levels of intracellular GSH.Multiple analyses selected 87 genes differentially expressed between normal AA and CA (P<0.01). The most relevant genes expressed in AA were categorized by function, including: signal transduction, response to stress, ECM genes, migration and cell adhesion.These data show that normal astrocytes from AA and CA normal donors display distinct expression profiles that impact astrocyte functions in the ONH. Our data suggests that differences in gene expression in ONH astrocytes may be specific to the development and/or progression of glaucoma in AA

Adsorption-oriented processes using conventional and non-conventional adsorbents for wastewater treatment

International audienceThe removal of contaminants from wastewaters is a matter of great interest in the field of water pollution. Amongst the numerous techniques of contaminant removal, adsorption using solid materials (named adsorbents) is a simple, useful and effective process. The adsorbent may be of mineral, organic or biological origin. Activated carbon is the preferred material at industrial scale and is extensively used not only for removing pollutants from wastewater streams but also for adsorbing contaminants from drinking water sources (e.g. rivers, lakes or reservoirs). However, its widespread use is restricted due to high cost. In the last three decades, numerous approaches have been studied for the development of cheaper and more effective adsorbents capable to eliminate pollutants at trace levels. This chapter gives a general overview of liquid-solid adsorption processes using conventional and non-conventional materials for pollutant removal. It outlines some of the principles of adsorption and proposes a classification for the different types of materials. Finally, the chapter discusses different mechanisms involved in the adsorption phenomena