17 research outputs found
The perfluoroalkyl carboxylic acids (PFCAs) and perfluoroalkane sulfonates (PFSAs) contamination level in spices
VAMP3/Syb and YKT6 are required for the fusion of constitutive secretory carriers with the plasma membrane
The cellular machinery required for the fusion of constitutive secretory vesicles with the plasma membrane in metazoans remains poorly defined. To address this problem we have developed a powerful, quantitative assay for measuring secretion and used it in combination with combinatorial gene depletion studies in Drosophila cells. This has allowed us to identify at least three SNARE complexes mediating Golgi to PM transport (STX1, SNAP24/29 and Syb; STX1, SNAP24/29 and YKT6; STX4, SNAP24 and Syb). RNAi mediated depletion of YKT6 and VAMP3 in mammalian cells also blocks constitutive secretion suggesting that YKT6 has an evolutionarily conserved role in this process. The unexpected role of YKT6 in plasma membrane fusion may in part explain why RNAi and gene disruption studies have failed to produce the expected phenotypes in higher eukaryotes
The importance of Antarctic krill in biogeochemical cycles
Antarctic krill (Euphausia superba) are swarming, oceanic crustaceans, up to two inches long, and best known as prey for whales and penguins – but they have another important role. With their large size, high biomass and daily vertical migrations they transport and transform essential nutrients, stimulate primary productivity and influence the carbon sink. Antarctic krill are also fished by the Southern Ocean’s largest fishery. Yet how krill fishing impacts nutrient fertilisation and the carbon sink in the Southern Ocean is poorly understood. Our synthesis shows fishery management should consider the influential biogeochemical role of both adult and larval Antarctic krill
The importance of claudin-7 palmitoylation on membrane subdomain localization and metastasis-promoting activities
Determination of 66 pesticide residues in livestock products using QuEChERS and GC–MS/MS
Urinary Lipidomics:evidence for multiple sources and sexual dimorphism in healthy individuals
The inhibitory effect of minocycline on radiation-induced neuronal apoptosis via AMPKα1 signaling-mediated autophagy
The south-western Black Forest and the Upper Rhine Graben Main Border Fault : thermal history and hydrothermal fluid flow
The thermal history of the south-westernmost Black Forest (Germany) and the adjacent Upper Rhine Graben were constrained by a combination of apatite and zircon fission-track (FT) and microstructural analyses. After intrusion of Palaeozoic granitic plutons in the Black Forest, the thermal regime of the studied area re-equilibrated during the Late Permian and the Mesozoic, interrupted by enhanced hydrothermal activity during the Jurassic. At the eastern flank of the Upper Rhine Graben along the Main Border Fault the analysed samples show microstructural characteristics related to repeated tectonic and hydrothermal activities. The integration of microstructural observations of the cataclastic fault gouge with the FT data identifies the existence of repeated tectonic-related fluid flow events characterised by different thermal conditions. The older took place during the Variscan and/or Mesozoic time at temperatures lower than 280A degrees C, whereas the younger was probably contemporary with the Cenozoic rifting of the Upper Rhine Graben at temperatures not higher than 150A degrees C