Isolation And Characterization Of Microsatellite Loci In Sisyrinchium (iridaceae) And Cross Amplification In Other Genera

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Recent phylogenetic studies on Sisyrinchium strongly suggest that species classified in section Hydastylus and section Viperella belong to a single group of plants in recent adaptive radiation (Clade IV). These species neither present clear morphological differentiation among them nor show clear identification using DNA barcode markers. Thus, the main goal of this study was to develop a set of polymorphic microsatellite markers compatible for representative species of both sections to ensure variability that could be revealed by SSR markers. Therefore, microsatellite primers were isolated and characterized for Sisyrinchium palmifolium and S. marchioides. In addition, transferability of the developed primers was tested in Iridoideae, primarily in closely related species of Sisyrinchium. Sixteen microsatellite loci were developed from enriched genomic libraries, of which ten were polymorphic. GST values indicated higher differentiation among subpopulations of S. palmifolium than those from S. marchioides. Major transferability was obtained using primers isolated from S. marchioides. All primers exhibited higher rates of cross-amplification for species belonging to Clade IV of Sisyrinchium, as well as to the genera Calydorea and Herbertia. These developed microsatellite markers can be used as an efficient tool for characterization of genetic variability in species belonging to Iridoideae, as well as for studies on population dynamics, genetic structure, and mating system in other Sisyrinchium species.153Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

A preliminary approach to the phylogeny of the genus Paspalum (Poaceae).

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Utilização de microssatélites e RAPD na caracterização molecular de acessos de paspalum urvillei steudel

Este estudo foi realizado com o objetivo de verificar a diversidade genética na coleção de acessos de P. urvillei do Departamento de Plantas Forrageiras e Agrometeorologia(DPFA) da Faculdade de Agronomia (UFRG) visando sua utilização em futuros trabalhos de seleção. Foram avaliados 64 acessos provenientes do Rio Grande do Sul, 1 de Xanxerê, Santa Catarina, três de Curitiba, Paraná, e 1 da Argentina. A diversidade genética foi analisada por meio de marcadores do tipo RAPD e SSR. Utilizaram-se dez primers para marcadores RAPD, o que possibilitou obter 56 bandas polimórficas e 11 grupos no dendrograma com similaridade média de 0,70. Na técnica de SSR, foram utilizados sete primers e obtidas 28 bandas polimórficas, formando sete grupos no dendrograma com similaridade média de 0,66. Ambos os marcadores foram eficientes para o agrupamento de acessos coletados. O uso de maior número de primers para gerar mais bandas polimórficas foi necessário para obtenção de fingerprints genômicos dos indivíduos similares. Os dendrogramas gerados neste estudo dão subsídios para futuros cruzamentos de gerações parentais contrastantes ou similares no melhoramento de Paspalum urvillei.The aim of this study was to estimate the genetic diversity among accesses of P. urvillei of Departamento de Plantas Forrageiras e Agrometeorologia (DPFA) of the College of Agronomy – UFRGS and to evaluate their use in selection programs. Sixty four accesses from different cities of the Southern Region of Brazil (Rio Grande do Sul, Santa Catarina and Parana States) and from Argentine were analyzed by RAPD and SSR molecular markers. Ten primers of RAPD markers were used and resulted in 56 polymorphic bands and 11 groups in a dendrogram with average similarity 0.70. Seven primers were used for the SSR technique and resulted in 28 polymorphic bands and seven groups in a dendrogram with average similarity 0.66. Both markers were efficient on grouping the accesses collected across the Southern region. A large number of primers was required to generate additional polymorphic bands to get genomic fingerprints of similar individuals. The dendrograms obtained from this study provided significant information for designing crossing strategies of parental generations in breeding programs of P. urvillei