2,384 research outputs found
Canine parvovirus and pseudorabies virus coinfection as a cause of death in a wolf (Canis lupus) from southern Italy
Pseudorabies virus (PRV) or suid herpesvirus 1 (SHV-1) is the causative agent of
Aujeszky's disease, a highly contagious viral infection which causes neurological fatal
illness in mammals other than suids. Here we report a case of a young wolf (Canis
lupus) of around 2 years found dead by a hunter in the province of Avellino, Campania
Region. Necropsy showed pathological findings consistent with encephalitis and gastroenteritis.
Organs were analysed by microbiological and molecular investigations
following standard procedures to ascertain the possible cause of death. Real-time
PCR revealed the presence of PRV in the brain and of canine parvovirus 2b in organs
like intestine, liver, brain, kidney and pancreas. Death probably occurred very shortly
after SHV-1 infection in an animal already weakened by parvovirosis
Should I and Can I?: a mixed methods study of clinician beliefs and attitudes in the management of lifestyle risk factors in primary health care
BackgroundPrimary health care (PHC) clinicians have an important role to play in addressing lifestyle risk factors for chronic diseases. However they intervene only rarely, despite the opportunities that arise within their routine clinical practice. Beliefs and attitudes have been shown to be associated with risk factor management practices, but little is known about this for PHC clinicians working outside general practice. The aim of this study was to explore the beliefs and attitudes of PHC clinicians about incorporating lifestyle risk factor management into their routine care and to examine whether these varied according to their self reported level of risk factor management.MethodsA cross sectional survey was undertaken with PHC clinicians (n = 59) in three community health teams. Clinicians\u27 beliefs and attitudes were also explored through qualitative interviews with a purposeful sample of 22 clinicians from the teams. Mixed methods analysis was used to compare beliefs and attitudes for those with high and low levels of self reported risk factor management.ResultsRole congruence, perceived client acceptability, beliefs about capabilities, perceived effectiveness and clinicians\u27 own lifestyle were key themes related to risk factor management practices. Those reporting high levels of risk factor screening and intervention had different beliefs and attitudes to those PHC clinicians who reported lower levels.ConclusionPHC clinicians\u27 level of involvement in risk factor management reflects their beliefs and attitudes about it. This provides insights into ways of intervening to improve the integration of behavioural risk factor management into routine practice
RNAseq Analyses Identify Tumor Necrosis Factor-Mediated Inflammation as a Major Abnormality in ALS Spinal Cord
ALS is a rapidly progressive, devastating neurodegenerative illness of adults that produces disabling weakness and spasticity arising from death of lower and upper motor neurons. No meaningful therapies exist to slow ALS progression, and molecular insights into pathogenesis and progression are sorely needed. In that context, we used high-depth, next generation RNA sequencing (RNAseq, Illumina) to define gene network abnormalities in RNA samples depleted of rRNA and isolated from cervical spinal cord sections of 7 ALS and 8 CTL samples. We aligned \u3e50 million 2X150 bp paired-end sequences/sample to the hg19 human genome and applied three different algorithms (Cuffdiff2, DEseq2, EdgeR) for identification of differentially expressed genes (DEGâs). Ingenuity Pathways Analysis (IPA) and Weighted Gene Co-expression Network Analysis (WGCNA) identified inflammatory processes as significantly elevated in our ALS samples, with tumor necrosis factor (TNF) found to be a major pathway regulator (IPA) and TNFα-induced protein 2 (TNFAIP2) as a major network âhubâ gene (WGCNA). Using the oPOSSUM algorithm, we analyzed transcription factors (TF) controlling expression of the nine DEG/hub genes in the ALS samples and identified TFâs involved in inflammation (NFkB, REL, NFkB1) and macrophage function (NR1H2::RXRA heterodimer). Transient expression in human iPSC-derived motor neurons of TNFAIP2 (also a DEG identified by all three algorithms) reduced cell viability and induced caspase 3/7 activation. Using high-density RNAseq, multiple algorithms for DEG identification, and an unsupervised gene co-expression network approach, we identified significant elevation of inflammatory processes in ALS spinal cord with TNF as a major regulatory molecule. Overexpression of the DEG TNFAIP2 in human motor neurons, the population most vulnerable to die in ALS, increased cell death and caspase 3/7 activation. We propose that therapies targeted to reduce inflammatory TNFα signaling may be helpful in ALS patients
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Z boson production in Pb+Pb collisions at âSnn = 5.02 TeV measured by the ATLAS experiment
The production yield of Z bosons is measured in the electron and muon decay channels in Pb+Pb collisions at âS = 5.02 TeV with the ATLAS detector. Data from the 2015 LHC run corresponding to an integrated luminosity of 0.49 nb are used for the analysis. The Z boson yield, normalised by the total number of minimum-bias events and the mean nuclear thickness function, is measured as a function of dilepton rapidity and event centrality. The measurements in Pb+Pb collisions are compared with similar measurements made in proton-proton collisions at the same centre-of-mass energy. The nuclear modification factor is found to be consistent with unity for all centrality intervals. The results are compared with theoretical predictions obtained at next-to-leading order using nucleon and nuclear parton distribution functions. The normalised Z boson yields in Pb+Pb collisions lie 1-3Ï above the predictions. The nuclear modification factor measured as a function of rapidity agrees with unity and is consistent with a next-to-leading-order QCD calculation including the isospin effect. nn -
Measurement of J/Ï production in association with a W ± boson with pp data at 8 TeV
A measurement of the production of a prompt J/Ï meson in association with a W± boson with W± â ΌΜ and J/Ï â ÎŒ+ÎŒâ is presented for J/Ï transverse momenta in the range 8.5â150 GeV and rapidity |yJ/Ï| < 2.1 using ATLAS data recorded in 2012 at the LHC. The data were taken at a proton-proton centre-of-mass energy of s = 8 TeV and correspond to an integrated luminosity of 20.3 fbâ1. The ratio of the prompt J/Ï plus W± cross-section to the inclusive W± cross-section is presented as a differential measurement as a function of J/Ï transverse momenta and compared with theoretical predictions using different double-parton-scattering cross-sections. [Figure not available: see fulltext.]
Search for flavour-changing neutral currents in processes with one top quark and a photon using 81 fbâ1 of pp collisions at s=13TeV with the ATLAS experiment
A search for flavour-changing neutral current (FCNC) events via the coupling of a top quark, a photon, and an up or charm quark is presented using 81 fbâ1 of protonâproton collision data taken at a centre-of-mass energy of 13 TeV with the ATLAS detector at the LHC. Events with a photon, an electron or muon, a b-tagged jet, and missing transverse momentum are selected. A neural network based on kinematic variables differentiates between events from signal and background processes. The data are consistent with the background-only hypothesis, and limits are set on the strength of the tqÎł coupling in an effective field theory. These are also interpreted as 95% CL upper limits on the cross section for FCNC tÎł production via a left-handed (right-handed) tuÎł coupling of 36 fb (78 fb) and on the branching ratio for tâÎłu of 2.8Ă10â5 (6.1Ă10â5). In addition, they are interpreted as 95% CL upper limits on the cross section for FCNC tÎł production via a left-handed (right-handed) tcÎł coupling of 40 fb (33 fb) and on the branching ratio for tâÎłc of 22Ă10â5 (18Ă10â5)
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