Nonlinear modal testing performed by pulsed-air jet excitation system

This paper presents a novel approach for testing structural component to nonlinear vibrations. Nowadays, nonlinear testing is mainly carried out by using electromagnetic shakers. These are efficient and powerful excitation systems which transmit the force by a rigid stinger and can be driven by different excitation signals. The rigid connection contributes to create mechanical impedance mismatch between the shaker and the test structure thus reducing the efficiency of the driving force. An alternative solution to shakers is represented by use of a pulsed air jet excitation method, which drives the force by a pulsed air-jets and therefore contactless. This condition eliminates the mechanical impedance mismatch with the test structure and the excitation can be more efficient than the one created by shakers. The pulsed air-jet excitation system is used to study nonlinear vibrations of composites components. These were designed to be mock-ups of fan blades the layup of which was varied for the three types of components used in this work. Tests were carried out by performing forced response and free decay measurements. The free decay type of test revealed interesting results and the novelty of using such an exciter for nonlinear testing. The major novelty consists of interrupting the air flow from a steady state condition and let happen the free decay, all these without experiencing undesired dynamics as experienced by contact excitatio

In silico Experimentation of Glioma Microenvironment Development and Anti-tumor Therapy

Tumor cells do not develop in isolation, but co-evolve with stromal cells and tumor-associated immune cells in a tumor microenvironment mediated by an array of soluble factors, forming a complex intercellular signaling network. Herein, we report an unbiased, generic model to integrate prior biochemical data and the constructed brain tumor microenvironment in silico as characterized by an intercellular signaling network comprising 5 types of cells, 15 cytokines, and 69 signaling pathways. The results show that glioma develops through three distinct phases: pre-tumor, rapid expansion, and saturation. We designed a microglia depletion therapy and observed significant benefit for virtual patients treated at the early stages but strikingly no therapeutic efficacy at all when therapy was given at a slightly later stage. Cytokine combination therapy exhibits more focused and enhanced therapeutic response even when microglia depletion therapy already fails. It was further revealed that the optimal combination depends on the molecular profile of individual patients, suggesting the need for patient stratification and personalized treatment. These results, obtained solely by observing the in silico dynamics of the glioma microenvironment with no fitting to experimental/clinical data, reflect many characteristics of human glioma development and imply new venues for treating tumors via selective targeting of microenvironmental components

Light and Temperature Signalling at the Level of CBF14 Gene Expression in Wheat and Barley

The wheat and barley CBF14 genes have been newly defined as key components of the light quality-dependent regulation of the freezing tolerance by the integration of phytochrome-mediated light and temperature signals. To further investigate the wavelength dependence of light-induced CBF14 expression in cereals, we carried out a detailed study using monochromatic light treatments at an inductive and a non-inductive temperature. Transcript levels of CBF14 gene in winter wheat Cheyenne, winter einkorn G3116 and winter barley Nure genotypes were monitored. We demonstrated that (1) CBF14 is most effectively induced by blue light and (2) provide evidence that this induction does not arise from light-controlled CRY gene expression. (3) We demonstrate that temperature shifts induce CBF14 transcription independent of the light conditions and that (4) the effect of temperature and light treatments are additive. Based on these data, it can be assumed that temperature and light signals are relayed to the level of CBF14 expression via separate signalling routes

Investigating Nonlinearities in a Demo Aircraft Structure under Sine Excitation

Developing on the basic idea behind parametric and non-parametric identification of nonlinear systems, another case study\ua0on integrating system identification and finite element modelling of nonlinear structures is presented.\ua0The first step, which is the focus of this paper, involves\ua0using acquired\ua0input and output data to derive an experimental model for both the underlying linear model and nonlinear model of the proposed structure, no information about the system is required and only the applied excitations and corresponding accelerations are implemented in the nonlinear identification step. The\ua0proposed case study\ua0is demonstrated on a nonlinear simple metallic plane assembly with\ua0localized stiffness and damping\ua0nonlinearities; in this case, an updated linear finite element model of the structure is derived and the nonlinearities experimentally characterised