L1 sequence of a new human papillomavirus type-58 variant associated with cervical intraepithelial neoplasia

The present study on molecular characterization of a human papillomavirus (HPV) isolated in Central Brazil describes the L1 gene sequence from a new variant of HPV-58, the isolate Bsb-02. The sample was from a smear obtained from a woman with cervical intraepithelial neoplasia grade II. The whole L1 gene from isolate Bsb-02 was sequenced automatically, showing 99.1% nucleotide identity with the gene from the HPV-58 reference. The clustering between Bsb-02 and HPV-58 reference sequence was also supported by phylogenetic analysis. Fourteen nucleotide substitutions were observed: eight were synonymous and six were associated with amino acid substitutions. A10V and V144I have not been previously described. At GenBank, the only complete L1 sequence from HPV-58 in addition to the HPV-58 reference one is that of Bsb-02. These data provide information that may be relevant to HPV diagnosis and to rational vaccine strategies. HPV variants may also be associated with host immune responses and with the risk of cervical neoplasia

COMPARTIMENTAÇÃO MORFOPEDOLÓGICA DA MICRORREGIÃO DE QUIRINÓPOLIS, GOIÁS

O texto apresenta uma análise integrada dos atributos físicos da Microrregião de Quirinópolis, Goiás, para a determinação dos Compartimentos Morfopedológicos (CMP). A metodologia adotada foi de acordo com Castro e Salomão (2000), utilizando as características semelhantes da geomorfologia, pedologia, geologia e hipsometria para a delimitação dos compartimentos. Como resultado da classificação, foi possível identificar cinco CMP distintos

Assessment of Baccharis trimera (Less.) DC. essential oil content in different packages during storage

The use of medicinal plants as therapeutic source is ancient and has increased in value in recent years for both the population and the science. Several national and international companies use raw plant material in the preparation of their products, making essential the commercial cultivation and the post-harvest processing of these species. The aim of this study was to evaluate the essential oil content of [Baccharis trimera (Less.) DC.] in three different packages over one year of storage. During storage, Kraft-paper package best preserved the integrity of some physical and chemical characteristics of the product and maintained the oil content for one year of storage.O uso de plantas medicinais como fonte terapêutica é milenar e nos últimos anos o seu valor tem aumentado, tanto para a população quanto para a ciência. Várias empresas nacionais e internacionais empregam matéria-prima vegetal na elaboração de seus produtos, o que torna indispensável o cultivo comercial e o beneficiamento pós-colheita destas espécies. O objetivo deste trabalho foi avaliar o teor de óleo essencial de carqueja [Baccharis trimera (Less.) DC.], em três embalagens diferentes ao longo de um ano de armazenamento. Observou-se que ao longo do armazenamento, a embalagem de papel Kraft foi a que melhor preservou a integridade de certas características físicas e químicas do produto, assim como, manteve o teor de óleo ao longo de um ano de armazenamento.545

Nova pesquisa sobre as coleções de Richard Spruce na Amazônia: uma colaboração Brasil-Reino Unido

The Northwest Amazon comprises a large region of equatorial forest on the border of Brazil, Colombia and Venezuela, which has been inhabited by indigenous peoples since the pre-colonial period. Today they occupy 80% of its area. Travellers such as Richard Spruce, who visited the region in the 1850s-1860s, described the vitality and dynamics of these populations, demonstrated by the size of their longhouses, their extensive inter-communal ceremonies, and their rich material culture. The biocultural objects and associated information collected by Richard Spruce constitute a unique point of reference for the useful plants, ethnobotany, anthropology and environmental history of the region. Housed at the Royal Botanic Gardens, Kew and the British Museum, both in London, this underresearched collection incorporates indigenous plant-based artefacts, samples of useful plant products, detailed archival notes on the use of plants by inhabitants of the Amazon, and accompanying herbarium voucher collections. This paper focusses on an ongoing research programme aimed at building capacity in Brazil to research, cataloguing and mobilising data from these biocultural collections, and developing these important resources for improved understanding of the useful and cultural properties of plants. It aims to build collaborative relationships, making biocultural collections and associated data freely accessible online, and above all to strengthen capacity of indigenous communities on the Rio Negro for autonomous research into material culture and plant use. We present the activities we have developed in the first two years of the programme. Workshops at Kew, Rio de Janeiro and São Gabriel da Cachoeira have enabled the Spruce collections to be fully digitised and artefacts made available through the Reflora portal (reflora.jbrj.gov. br). Training has been given in collection and study of biocultural objects, both to museum staff and representatives of indigenous communities, and a research agenda developed that focuses on better understanding of the shifting relationships between people and natural resources over the last 200 years. We discuss how a broad collaboration has led to constructive, culturally appropriate engagement with local communities, providing a portal into the world of scientific knowledge and helping to mobilise both scientific and indigenous knowledge in a mutually beneficial manner

Elephant grass silage inoculated with cellulolytic fungi isolated from rumen

ABSTRACT The objective was to evaluate the inoculation with Aspergillus terreus and/or Trichoderma longibrachiatum on fermentation, chemical and microbiological composition of elephant grass ‘Cameroon’ silage (Cenchrus purpureus). Treatments were A. terreus at 105 colony forming units (CFU)/g (AT15), T. longibrachiatum at 105 CFU/g (TL20), a mixture of both at 105 CFU/g (MIX), and a control group without inoculation (CONTR). The design was completely randomized with seven replicates. The MIX silage was most stable, while CONTR, AT15, and TL20, had lower dry matter losses. There was no effect of inoculation in the chemical composition of silages. Only MIX silage (4.40) had pH above the minimum of 4.2 for humid grass silage and above the control (4.05). Bacteria from Diplococcus genus was identified at the opening of TL20 and CONTR silages. After air exposure, the population of rods, Lactobacillus, and total lactic acid bacteria was higher in theTL20 and MIX. The inclusion of a T. longibrachiatum and A. terreus mixture increases dry mater loss and silage pH. T. longibrachiatum was more efficient in maintaining populations of total lactic acid bacteria after opening; therefore, this strain has potential as an additive for elephant grass ‘Cameroon’ silage

Spatial distribution and evaluation of risk factors for bovine neosporosis in Rondônia, Brazil

ABSTRACT Neospora caninum is an important worldwide parasite responsible for causing abortion in animals. Due to limited information on the occurrence of infection by this parasite in the state of Rondônia, Brazil, this study aimed to determine the seroprevalence and identify the risk factors associated with the infection in slaughtered cattle, from 19 municipalities distributed in seven microregions of the state. A total of 494 samples were obtained and subjected to anti-N. caninum antibodies, using the Indirect Immunofluorescence Reaction technique. Antibodies were detected in 5.06% (25/494) of the samples, in 30.30% (10/33) of farms, in nine municipalities located in four microregions of Rondônia. Of all the animals analyzed, 4.81% of the females (20/416) and 6.41% of the males (05/78) were seropositive for the parasite, with “abortion in the last 12 months” being considered an important risk factor for the occurrence of infection (OR = 9.54; p = 0.01). The present study points out the prevalence of anti-N. caninum antibodies in 5.06% of slaughtered animals and abortion as the main risk factor associated with infection by N. caninum, thus contributing to the elucidation of the epidemiology of this protozoan in Rondônia, Brazil