Laboratório de Microbiologia.

Laboratório de Microbiologia.info:eu-repo/semantics/publishedVersio

Structural traits of an eight year old secondary forest in the Central Amazon.

Presentation of the floristical composition, stratification and growth-form structure of the vegetation in the 1 ha secondary forest in Manaus-AM (Brasil). The variation of the vegetation within 1 ha area is analysed with the aid of multivariate analyses (PCA) of the floristical composition of the plots, and the resulting ordination model is displayed as a covariance biplot. Conclusions are drawn from the floristical and structural analyses concerning the key environmental factors leading to the vegetation studied, and the influence of the surveys on vegetation development, i.e., disturbance such as trampling and cutting of trees, is discussed

Evolução da caprinocultura brasileira no período de 1975 a 2003.

bitstream/item/35632/1/Evolucao-da-caprinocultura.pd

Evolução da caprinocultura brasileira no período de 1975 a 2003.

bitstream/CNPC/20242/1/cot66.pd

Evolução da ovinocultura brasileira no período de 1975 a 2003.

bitstream/CNPC/20243/1/cot67.pd

Differential mesenteric fat deposition in bovines fed on silage or concentrate is independent of glycerol membrane permeability

© The Animal Consortium 2011In the meat industry, the manipulation of fat deposition in cattle is of pivotal importance to improve production efficiency, carcass composition and ultimately meat quality. There is an increasing interest in the identification of key factors and molecular mechanisms responsible for the development of specific fat depots. This study aimed at elucidating the influence of breed and diet on adipose tissue membrane permeability and fluidity and their interplay on fat deposition in bovines. Two Portuguese autochthonous breeds, Alentejana and Barrosã, recognized as late- and early-maturing breeds, respectively, were chosen to examine the effects of breed and diet on fat deposition and on adipose membrane composition and permeability. Twenty-four male bovines from these breeds were fed on silage-based or concentrate-based diets for 11 months. Animals were slaughtered to determine their live slaughter and hot carcass weights, as well as weights of subcutaneous and visceral adipose depots. Mesenteric fat depots were excised and used to isolate adipocyte membrane vesicles where cholesterol content, fatty acid profile as well as permeability and fluidity were determined. Total accumulation of neither subcutaneous nor visceral fat was influenced by breed. In contrast, mesenteric and omental fat depots weights were higher in concentrate-fed bulls relative to silage-fed animals. Membrane fluidity and permeability to water and glycerol in mesenteric adipose tissue were found to be independent of breed and diet. Moreover, the deposition of cholesterol and unsaturated fatty acids, which may influence membrane properties, were unchanged among experimental groups. Adipose membrane lipids from the mesenteric fat depot of ruminants were rich in saturated fatty acids, and unaffected by polyunsaturated fatty acids dietary levels. Our results provide evidence against the involvement of cellular membrane permeability to glycerol on fat accumulation in mesenteric fat tissue of concentrate-fed bovines, which is consistent with the unchanged membrane lipid profile found among experimental groups.This study was supported by Fundação para a Ciência e a Tecnologia (FCT) through grant PTDC/CVT/2006/66114 and individual fellowships to Ana P. Martins (SFRH/BD/2009/65046), Ana S. H. Costa (SFRH/BD/2009/61068) and Susana V. Martins (SFRH/BPD/2009/63019). Paula A. Lopes is a researcher from the program ‘‘Ciência 2008’’ from FC

Evolução da ovinocultura brasileira no período de 1975 a 2003.

bitstream/item/35607/1/Evolucao-da-ovinocultura.pd

Eletroforese de isoenzimas de plântulas na identificação de espécies de Brachiaria.

Lotes de sementes de braquiaria comercializados no Brasil vem apresentando contaminacoes som sementes de outras especies pertencentes ao mesmo genero. Deste modo, uma das especies de braquiaria atuaria como planta infestante da outra no agroecossistema e a erradicacao da especie infestante seria dificultada pela agressividade caracteristca do genero e pela falta de seletividade dos herbecidas disponiveis no mercado

Molecular Evaluation of exons 8 and 22 of the SHANK3 gene in Autism Spectrum Disorders

Autism spectrum disorders are a group of neurodevelopmental disorders with a complex and heterogeneous etiology. Studies have shown that genetic factors play an important role in the aetiology of these diseases. Recently, de novo mutations, frameshifts and deletions have been described in the SHANK3 gene, also known as ProSAP2 gene, which encodes a synaptic scaffolding protein. All the participants of this study had normal karyotypes and underwent screening for Fragile-X syndrome. Subsequently, they were analyzed by direct sequencing of different points of exons 8 and 22 of the SHANK3 gene. None of the study participants presented with changes in these regions. These findings may be due to the fact that mutations, deletions and duplications of the SHANK3 gene are rare