The classification of submerged vegetation using hyperspectral MIVIS data

The aim of this research is to use hyperspectral MIVIS data to map the Posidonia oceanica prairies in a coastal lagoon (Stagnone di Marsala). It is approximately 12 km long and 2 km wide and is linked to the open sea by two shallow openings. This environment is characterised by prairies of phanerogams, the most common of which is Posidonia oceanica, an ideal habitat for numerous species of fish, molluscs and crustaceans. A knowledge of the distribution of submerged vegetation is useful to monitor the health of the lagoon. In order to classify the MIVIS imagery, the attenuation effects of the water column have been removed from the signal using Lyzenga’s technique. A comparison between classifications using indices obtained using band pairs from only the first spectrometer, and using band pairs of the first and second spectrometers, shows that the best classification is obtained from some indices derived from the first spectrometer. Field controls carried out in July 2002 were used to determine the training sites for the supervised classification. Twelve classes of bottom coverage were obtained from the classification, of which four are homogeneous and eight are mixed coverage. The methodology applied demonstrates that hyperspectral sensors can be used to effectively map submerged vegetation in shallow waters

Progression of motor deficits in glioma-bearing mice: impact of CNF1 therapy at symptomatic stages

Glioblastoma (GBM) is the most aggressive type of brain tumor. In this context, animal models represent excellent tools for the early detection and longitudinal mapping of neuronal dysfunction, that are critical in the preclinical validation of new therapeutic strategies. In a mouse glioma model, we developed sensitive behavioral readouts that allow early recognizing and following neurological symptoms. We injected GL261 cells into the primary motor cortex of syngenic mice and we used a battery of behavioral tests to longitudinally monitor the dysfunction induced by tumor growth. Grip strength test revealed an early onset of functional deficit associated to the glioma growth, with a significant forelimb weakness appearing 9 days after tumor inoculation. A later deficit was observed in the rotarod and in the grid walk tasks. Using this model, we found reduced tumor growth and maintenance of behavioral functions following treatment with Cytotoxic Necrotizing Factor 1 (CNF1) at a symptomatic stage. Our data provide a detailed and precise examination of how tumor growth reverberates on the behavioral functions of glioma-bearing mice, providing normative data for the study of therapeutic strategies for glioma treatment. The reduced tumor volume and robust functional sparing observed in CNF1-treated, glioma-bearing mice strengthen the notion that CNF1 delivery is a promising strategy for glioma therapy

La scheda interattiva di INNOVance per prodotti in laterizio

Anche per i laterizi la scheda tecnica di prodotto, sviluppata nell’ambito del pro getto INNOVance, assume un ruolo fondamentale a supporto della progettazione e della gestione delle informazioni nelle diverse fasi del processo edilizio. L’insieme di dati contenuti in ciascuna scheda sarà associato alla rappresentazione di oggetti innovativi di tipo BIM 'Building Information Model

Microenvironment in neuroblastoma: Isolation and characterization of tumor-derived mesenchymal stromal cells

Background: It has been proposed that mesenchymal stromal cells (MSCs) promote tumor progression by interacting with tumor cells and other stroma cells in the complex network of the tumor microenvironment. We characterized MSCs isolated and expanded from tumor tissues of pediatric patients diagnosed with neuroblastomas (NB-MSCs) to define interactions with the tumor microenvironment. Methods: Specimens were obtained from 7 pediatric patients diagnosed with neuroblastoma (NB). Morphology, immunophenotype, differentiation capacity, proliferative growth, expression of stemness and neural differentiation markers were evaluated. Moreover, the ability of cells to modulate the immune response, i.e. inhibition of phytohemagglutinin (PHA) activated peripheral blood mononuclear cells (PBMCs) and natural killer (NK) cytotoxic function, was examined. Gene expression profiles, known to be related to tumor cell stemness, Wnt pathway activation, epithelial-mesenchymal transition (EMT) and tumor metastasis were also evaluated. Healthy donor bone marrow-derived MSCs (BM-MSC) were employed as controls. Results: NB-MSCs presented the typical MSC morphology and phenotype. They showed a proliferative capacity superimposable to BM-MSCs. Stemness marker expression (Sox2, Nanog, Oct3/4) was comparable to BM-MSCs. NB-MSC in vitro osteogenic and chondrogenic differentiation was similar to BM-MSCs, but NB-MSCs lacked adipogenic differentiation capacity. NB-MSCs reached senescence phases at a median passage of P7 (range, P5-P13). NB-MSCs exhibited greater immunosuppressive capacity on activated T lymphocytes at a 1:2 (MSC: PBMC) ratio compared with BM-MSCs (p = 0.018). NK cytotoxic activity was not influenced by co-culture, either with BM-MSCs or NB-MSCs. Flow-cytometry cell cycle analysis showed that NB-MSCs had an increased number of cells in the G0-G1 phase compared to BM-MSCs. Transcriptomic profiling results indicated that NB-MSCs were enriched with EMT genes compared to BM-MSCs. Conclusions: We characterized the biological features, the immunomodulatory capacity and the gene expression profile of NB-MSCs. The NB-MSC gene expression profile and their functional properties suggest a potential role in promoting tumor escape, invasiveness and metastatic traits of NB cancer cells. A better understanding of the complex mechanisms underlying the interactions between NB cells and NB-derived MSCs should shed new light on potential novel therapeutic approaches

Thermal and Economic Efficiency of Progressive Retrofit Strategies for School Buildings by a Statistical Analysis based Tool

Design alternatives in air conditioned buildings may be easily compared just by summing the hourly consumption of primary energy, while quantitative approachs for bioclimatic design strategies are difficult to be assessed and compared. A actively heated and passively cooled school building is considered as an application field of a novel methodology to promote an informed choice about the retrofit strategies to be adopted for buildings, defined as the Gained Comfort Cost (GCC). A functional and significant unit (i.e. a classroom), is used to test different energy retrofit solutions and their performances were compared with a baseline, in terms of the capacity to reduce the indoor air temperature variation. The novel methodology is a visual tool allowing to understand the “distance” of indoor conditions from comfort; the retrofit strategies are promoted to reduce this distance considering however the associated costs (LCC) to deal with actual feasibility

The classification of submerged vegetation using hyperspectral MIVIS data

The aim of this research is to use hyperspectral MIVIS data to map the Posidonia oceanica prairies in a coastal lagoon (Stagnone di Marsala). It is approximately 12 km long and 2 km wide and is linked to the open sea by two shallow openings. This environment is characterised by prairies of phanerogams, the most common of which is Posidonia oceanica, an ideal habitat for numerous species of fish, molluscs and crustaceans. A knowledge of the distribution of submerged vegetation is useful to monitor the health of the lagoon. In order to classify the MIVIS imagery, the attenuation effects of the water column have been removed from the signal using Lyzenga's technique. A comparison between classifications using indices obtained using band pairs from only the first spectrometer, and using band pairs of the first and second spectrometers, shows that the best classification is obtained from some indices derived from the first spectrometer. Field controls carried out in July 2002 were used to determine the training sites for the supervised classification. Twelve classes of bottom coverage were obtained from the classification, of which four are homogeneous and eight are mixed coverage. The methodology applied demonstrates that hyperspectral sensors can be used to effectively map submerged vegetation in shallow waters.

Phase II study of the safety and efficacy of oral capecitabine in patients with platinum-pretreated advanced or recurrent cervical carcinoma

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