A importância de monitorar a qualidade da água na piscicultura.

Informacoes tecnicas sobre monitoracao da agua na piscicultura, em Manaus-AM (Brasil), envolvendo: temperatura, oxigenio dissolvido (O2D), Transparencia, pH, amonio, nebulosidade, nutrientes.bitstream/CPAA-2009-09/4510/1/IT_5_99.pd

Changes in hematological and biochemical parameters of tambaqui (Colossoma macropomum) parasitized by metazoan species.

The aim of this study was to evaluate the impacts of metazoan parasites on hematological and biochemical parameters and relative condition factor of tambaqui (Colossoma macropomum) farmed in northern Brazil. A total of 32 juvenile fish were captured from a commercial fish farm located in the municipality of Rio Preto da Eva, Amazonas state, Brazil. Parasite prevalence was 100% for Anacanthorus spathulatus, Mymarothecium boegeri and Notozothecium janauachensis, 100% for Neoechinorhynchus buttnerae and 53.13% for Dolops geayi. The greatest mean parasite intensity was found in acantocephalans followed by monogeneans and branchiuran crustaceans. A negative correlation was observed between abundance of N. buttnerae and hematocrit percentage, hemoglobin concentration, total thrombocyte count and glucose and between abundance of the monogenean and glucose concentration. Parasitic infections caused damage in tambaqui in terms of the observed hematological parameters that were characterized by hypochromic anemia and thrombocytopenia, which are important parameters to be used in parasitic diagnosis. This study is the first record of the occurrence of Dolops geayi in farmed tambaqui in the Amazon

Antiparasitic efficacy and blood effects of formalin on Arapaima gigas (Pisces: Arapaimidae).

This study evaluated the in vitro and in vivo antiparasitic efficacy of formalin against Dawestrema cycloancistrium, the effects on the physiological response of Arapaima gigas and the residual action on fish muscle after 96h of exposure. As regards the in vitro assay, 0, 22, 44, 66, 88, 110, 330, 660 and 880 mg L-1 formalin were tested. After 1h of exposure to 660 and 880 mg L-1 formalin, there was a 100% mortality of D. cycloancistrium as well as after 2h of exposure at 330 and 110 mg L-1 and 3h of exposure at 44, 66 and 88 mg L-1. Concerning the in vivo test, when fish were exposed to formalin at 0, 220, 330, 440 and 550 mg L-1, there was 100% survival at all concentrations and exposure times evaluated. Baths of 1h with 440 and 550 mg L-1 formalin showed 93.3% and 99.3% efficacy respectively. However, the baths of 12h with 55 and 66 mg L-1 formalin had the efficacy of 44.5% and 55.5% respectively. In 1h baths with 220, 330, 440 and 550 mg L-1 formalin, hematocrit, hemoglobin, number of total erythrocytes, mean corpuscular volume, mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, plasma glucose levels, cortisol, total proteins, chloride, calcium, sodium, potassium and magnesium of the fish presented no differences in relation to the control values. However, in baths of 12h with 33, 44, 55 and 66 mg L-1 formalin, there was a decrease in hematocrit, plasma levels of calcium and chloride, and increased levels of glucose and cortisol, depending on the concentration of formalin used. In the fish muscle, the formalin residue decreased after 96h in all concentrations and periods evaluated, returning to values close to the control ones. The results indicate that formalin had its efficacy successfully proved in the treatment against D. cycloancistrium at higher concentrations such as 440 and 550 mg L-1 formalin and shorter exposure time (1h) without compromising fish homeostasis and consumer food safety. Statement of relevance: The manuscript represents original research on use of formalin in vitro and in vivo for treating infection by monogenoidean Dawestrema cycloancistrium in Arapaima gigas, the giant fish from Amazon. In the fish, muscle the residue levels of formalin after exposure was also investigated. Formalin have efficacy in the treatment against D. cycloancistrium at higher concentrations (440 and 550 mg.L-1) of formalin and shorter exposure time (1h) and without compromising A. gigas homeostasis and consumer food safety

Parâmetros hematológicos e bioquímicos de tambaqui (Colossoma macropomum) parasitados por metazoários.

O objetivo deste estudo foi avaliar o impacto do parasitismo por metazoários sobre os parâmetros hematológicos, bioquímicos e fator de condição de tambaquis (Colossoma macropomum) cultivados na região Norte do Brasil.AQUACIÊNCIA 2018

Elucidation of chiral Recognition mechanism of alpha-amino acids using ligand exchange high performance liquid chromatography

Ligand exchange HPLC technique was applied to resolve chiral separation of aliphatic side chain aminoacid racemates. Chiral selector was copper L-phenylalaninate (II) and the results showed the elution of D enantiomer followed by L form. Considering the 3-point interaction concept, a mechanism of chiral recognition was proposed, in which no change of configuration would follow the formation of pseudo-homochiral and heterochiral complexes. To prove the reliability of this mechanism, the trans configuration of homochiral complex had to be more stable than the cis form, which was confirmed by DFT-B3LYP calculation in gas phase. The infrared frequencies were also calculated and the comparison with the subtracted and deconvoluted spectrum of the in-solution complex also pointed to the presence of the trans diasteroisomer

Parasitos do matrinxã Brycon amazonicus Spix & Agassiz, 1829 (Characidae: Bryconinae) na Amazônia Central.

Neste capítulo descrevem-se os parasitos de matrinxã Brycon amazonicus cultivado em diferentes modalidades na Amazônia Central. Estudos demonstram que com a intensificação do cultivo deste peixe os problemas com as infecções parasitárias também podem mostrar incremento, devido às condições sanitárias, transporte e manejo inadequados. O transporte de peixes é uma maneira rápida de propagação de doenças, quando os cuidados com profilaxia e quarentena não são mantidos na piscigranja. Nestes estabelecimentos de cultivo, os estudos de parasitofauna podem auxiliar no controle e proliferação dos parasitos, evitando assim perdas econômicas significativas para o produtor.bitstream/item/211044/1/CPAF-AP-Parasitos-de-matrinxa.pd

Structural and Luminescence Properties of Silica-Based Hybrids Containing New Silylated-Diketonato Europium(III) Complex

A new betadiketonate ligand displaying a trimethoxysilyl group as grafting function and a diketone moiety as complexing site (TTA-Si = 4,4,4-trifluoro-2-(3-trimethoxysilyl)propyl)-1-3-butanedione (C4H3S)COCH[(CH2)3Si(OCH3)3]COCF3) and its highly luminescent europium(III) complex [Eu(TTA-Si)3] have been synthesized and fully characterized. Luminescent silica-based hybrids have been prepared as well with this new complex grafted on the surface of dense silica nanoparticles (28 (+/-3 nm) or on mesoporous silica particles. The covalent bonding of Eu(TTA-Si)3 inside the core of uniform silica nanoparticles (40 (+/- 5 nm) was also achieved. Luminescence properties are discussed in relation to the europium chemical environment involved in each of the three hybrids. The general methodology proposed allowed high grafting ratios and overcame chelate release and tendency to agglomeration, and it could be applied to any silica matrix (in the core or at the surface, nanosized or not, dense or mesoporous) and therefore numerous applications such as luminescent markers and luminophors could be foreseen

Evidence of the participation of electronic excited states in the mechanism of positronium formation in substitutional Tb1-xEux(dpm)(3) solid solutions studied by optical and positron annihilation spectroscopies

Positronium formation in the bimary molecular solid solutions Tb1-xEux (dpm)(3) (dpm = dipivaloylmethanate) has been investigated. A strong linear correlation between the D-5(4) Tb(III) energy level excited state lifetime and the positronium formation probability has been observed. This correlation indicates that the ligand-to-metal charge transfer LMCT states act in both luminescence quenching and positronium formation inhibition, as previously proposed. A kinetic mechanism is proposed to explain this correlation and shows that excited electronic states have a very important role in the positronium formation mechanism.CNPqCNPqFAPEMIGFAPEMIGFAPESPFAPESPRENAMI (National Project)RENAMI (National Project