ATP synthase: from single molecule to human bioenergetics

ATP synthase (FoF1) consists of an ATP-driven motor (F1) and a H+-driven motor (Fo), which rotate in opposite directions. FoF1 reconstituted into a lipid membrane is capable of ATP synthesis driven by H+ flux. As the basic structures of F1 (α3β3γδε) and Fo (ab2c10) are ubiquitous, stable thermophilic FoF1 (TFoF1) has been used to elucidate molecular mechanisms, while human F1Fo (HF1Fo) has been used to study biomedical significance. Among F1s, only thermophilic F1 (TF1) can be analyzed simultaneously by reconstitution, crystallography, mutagenesis and nanotechnology for torque-driven ATP synthesis using elastic coupling mechanisms. In contrast to the single operon of TFoF1, HFoF1 is encoded by both nuclear DNA with introns and mitochondrial DNA. The regulatory mechanism, tissue specificity and physiopathology of HFoF1 were elucidated by proteomics, RNA interference, cytoplasts and transgenic mice. The ATP synthesized daily by HFoF1 is in the order of tens of kilograms, and is primarily controlled by the brain in response to fluctuations in activity

Ovário-histerectomia laparoscópica em felinos hígidos: estudo comparativo de três métodos de hemostasia Laparoscopic ovariohysterectomy in healthy felines: comparative study of three hemostatic methods

Foram avaliadas 24 gatas, hígidas, sem raça definida, distribuídas em três grupos de oito animais. Descreveu-se o acesso laparoscópico para ovariossalpingo-histerectomia (OSH) e comparou-se o uso do eletrocautério bipolar (grupo I), do clipe de titânio (grupo II) e da ligadura com fio de sutura (grupo III) para a oclusão dos vasos ovarianos e uterinos. Hemorragia e enfisema subcutâneo foram as principais complicações observadas no transoperatório e hematoma subcutâneo e deiscência de sutura, as do pós-operatório. O procedimento cirúrgico e a técnica operatória mostraram-se viáveis nos três grupos. O uso do eletrocautério bipolar apresentou vantagens na comparação com os outros métodos de hemostasia.<br>Twenty-four healthy female mongrel cats were submitted to ovaryhisterectomy and distributed into three groups of eight animals each: (I) bipolar electrical cautery, (II) titanium clips, and (III) suture ligature for the occlusion of ovarian and uterine vessels, which were compared. The surgical procedure and the operation technique were viable in all three groups. Hemorrhagia and subcutaneous emphysema were the main intraoperative complications, and hematoma and suture dehiscence in the postoperative. The bipolar electrical cautery procedure is shown to be comparatively successful regarding the other studied methods for hemostasia

Diabetes in sub-Saharan Africa: from clinical care to health policy

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