Elastocapillary folding of three dimensional micro-structures using water pumped through the wafer via a silicon nitride tube

In this paper we present the first investigation of a batch method for folding of threedimensional micrometer-sized silicon nitride structures by capillary forces. Silicon nitride tubes have been designed and fabricated using DRIE at the center of the planar origami patterns of the structures. Water is brought to the structures by pumping the liquid through the wafer via those tubes. Isolated micro-structures were successfully folded using this method. The potential of this technique for batch self-assembly is discussed

Self-Diffusion of a Polymer Chain in a Melt

Self-diffusion of a polymer chain in a melt is studied by Monte Carlo simulations of the bond fluctuation model, where only the excluded volume interaction is taken into account. Polymer chains, each of which consists of $N$ segments, are located on an $L \times L \times L$ simple cubic lattice under periodic boundary conditions, where each segment occupies $2 \times 2 \times 2$ unit cells. The results for $N=32, 48, 64, 96, 128, 192, 256, 384$ and 512 at the volume fraction $\phi \simeq 0.5$ are reported, where $L = 128$ for $N \leq 256$ and L=192 for $N \geq 384$. The $N$-dependence of the self-diffusion constant $D$ is examined. Here, $D$ is estimated from the mean square displacements of the center of mass of a single polymer chain at the times larger than the longest relaxation time. From the data for $N = 256$, 384 and 512, the apparent exponent $x_{\rm d}$, which describes the apparent power law dependence of $D$ on $N$ as $D \propto N^{- x_{\rm d}}$, is estimated as $x_{\rm d} \simeq 2.4$. The ratio $D \tau / < R_{\rm e}^{2} >$ seems to be a constant for $N = 192, 256, 384$ and 512, where $\tau$ and $<R_{\rm e}^{2}>$ denote the longest relaxation time and the mean square end-to-end distance, respectively.Comment: 4 pages, 3 figures, submitted to J. Phys. Soc. Jp

Kinematic parameters contributing to the production of spin in elite finger spin bowling

The aim of this study was to identify the key kinematic parameters which contribute to higher spin rates in elite finger spin bowling. Kinematic data were collected for twenty-three elite male finger spin bowlers with thirty kinematic parameters calculated for each delivery. Stepwise linear regression and Pearson product moment correlations were used to identify kinematic parameters linked to spin rate. Pelvis orientation at front foot contact (r = 0.674, p < 0.001) and ball release (r = 0.676, p < 0.001) were found to be the biggest predictors of spin rate, with both individually predicting 43% of the observed variance in spin rate. Other kinematic parameters correlated with spin rate included: shoulder orientation at ball release (r = 0.462, p = 0.027), and pelvis-shoulder separation angle at front foot contact (r = 0.521, p = 0.011). The bowlers with the highest spin rates adopted a mid-way pelvis orientation angle, a larger pelvis-shoulder separation angle and a shoulder orientation short of side-on at front foot contact. The segments then rotated sequentially, starting with the pelvis and finishing with the pronation of the forearm. This knowledge can be translated to coaches to provide a better understanding of finger spin bowling technique

Estudio agroeconomico del proceso de produccion de frijol (Phaseolus vulgaris) en Colombia

A methodology is presented on how to study bean production processes and its on-farm application in Colombia. The main objectives are to identify the factors limiting production and productivity, availability and use of resources, credit, technical assistance, markets, pest and weed distribution and intensity, production costs, and crop profit. The study consists of various stages: definition of the problem, antecedents, interviews with technicians and administrators, bibliographic review, definition of objectives and variables, questionnaires, pilot and final surveys, selection of areas (Antioquia, Huila, Narino, and Valle del Cauca), municipalities, and producers (177 farms with an av. of 25 ha). The av. area of bean crops is 6 ha with great differences among states; more than 60 percent of the cultivation is shared with other crops such as maize, coffee, plantain, soybeans, cassava, aracacha, peas and potatoes. Except farmers of the Valle, and to a lesser extent, those that only cultivate beans in Huila, the great majority of cultivators did not have adequate mechanical equipment for preparation, planting, harvesting, and irrigation. Seventy percentage are landowners, 10 percent tenants, and 17 percent sharecroppers. It is considered that beans need less irrigation, should be harvested quickly, and that it is the crop that produces the greatest profit; however, it is considered to be the crop representing the highest risk. Planting systems are described: on flat rows; multiple cropping on ridges; multiple cropping without rows; multiple cropping in the same flat row; multiple cropping in different rows, and mixed multiple cropping. Only 12 percent of the cultivators use improved seed and during the study, 86, 48 and 34 percent planted red, black, and both types of beans, resp. Data are presented on the intensity of land use; principle pests, diseases, and weeds are evaluated. Information on costs, storage, and marketing are also included. (CIAT

Sedation improves early outcome in severely septic Sprague Dawley rats

Introduction Sepsis, a systemic inflammatory response to infective etiologies, has a high mortality rate that is linked both to excess cytokine activity and apoptosis of critical immune cells. Dexmedetomidine has recently been shown to improve outcome in a septic cohort of patients when compared to patients randomized to a benzodiazepine-based sedative regimen. We sought to compare the effects of dexmedetomidine and midazolam, at equi-sedative doses, on inflammation and apoptosis in an animal model of severe sepsis. Methods After central venous access, Sprague Dawley rats underwent cecal ligation and intestinal puncture (CLIP) with an 18 G needle without antibiotic cover and received either saline, or an infusion of comparable volume of saline containing midazolam (0.6 mg.kg(-1).h(-1)) or dexmedetomidine (5 ug.kg(-1).h(-1)) for 8 hours. Following baseline measurements and CLIP, blood was sampled for cytokine measurement (tumour necrosis factor (TNF)-alpha and interleukin (IL)-6; n = 4-6 per group) at 2, 4 and 5 hours, and animal mortality rate (MR) was monitored (n = 10 per group) every 2 hours until 2 hours had elapsed. In addition, spleens were harvested and apoptosis was assessed by immunoblotting (n = 4 per group). Results The 24 hour MR in CLIP animals (90%) was significantly reduced by sedative doses of either dexmedetomidine (MR = 20%) or midazolam (MR = 30%). While both sedatives reduced systemic levels of the inflammatory cytokine TNF-alpha (P &lt; 0.05); only dexmedetomidine reduced the IL-6 response to CLIP, though this narrowly missed achieving significance (P = 0.05). Dexmedetomidine reduced splenic caspase-3 expression (P &lt; 0.05), a marker of apoptosis, when compared to either midazolam or saline. Conclusions Sedation with midazolam and dexmedetomidine both improve outcome in polymicrobial severely septic rats. Possible benefits conveyed by one sedative regimen over another may become evident over a more prolonged time-course as both IL-6 and apoptosis were reduced by dexmedetomidine but not midazolam. Further studies are required to evaluate this hypothesis.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000272225600033&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Critical Care MedicineSCI(E)PubMed33ARTICLE4R1361

Canine distemper virus neutralization activity is low in human serum and it is sensitive to an amino acid substitution in the hemagglutinin protein

© 2015 Elsevier Inc.Serum was analyzed from 146 healthy adult volunteers in eastern Africa to evaluate measles virus (MV) and canine distemper virus (CDV) neutralizing antibody (nAb) prevalence and potency. MV plaque reduction neutralization test (PRNT) results indicated that all sera were positive for MV nAbs. Furthermore, the 50% neutralizing dose (ND50) for the majority of sera corresponded to antibody titers induced by MV vaccination. CDV nAbs titers were low and generally were detected in sera with high MV nAb titers. A mutant CDV was generated that was less sensitive to neutralization by human serum. The mutant virus genome had 10 nucleotide substitutions, which coded for single amino acid substitutions in the fusion (F) and hemagglutinin (H) glycoproteins and two substitutions in the large polymerase (L) protein. The H substitution occurred in a conserved region involved in receptor interactions among morbilliviruses, implying that this region is a target for cross-reactive neutralizing antibodies

Host genetics and viral load in primary HIV-1 infection: clear evidence for gene by sex interactions

© 2014, The Author(s).Research in the past two decades has generated unequivocal evidence that host genetic variations substantially account for the heterogeneous outcomes following human immunodeficiency virus type 1 (HIV-1) infection. In particular, genes encoding human leukocyte antigens (HLA) have various alleles, haplotypes, or specific motifs that can dictate the set-point (a relatively steady state) of plasma viral load (VL), although rapid viral evolution driven by innate and acquired immune responses can obscure the long-term relationships between HLA genotypes and HIV-1-related outcomes. In our analyses of VL data from 521 recent HIV-1 seroconverters enrolled from eastern and southern Africa, HLA-A*03:01 was strongly and persistently associated with low VL in women (frequency = 11.3 %, P  0.50). In a reduced multivariable model, age, sex, geography (clinical sites), previously identified HLA factors (HLA-B*18, B*45, B*53, and B*57), and the interaction term for female sex and HLA-A*03:01 collectively explained 17.0 % of the overall variance in geometric mean VL over a 3-year follow-up period (P < 0.0001). Multiple sensitivity analyses of longitudinal and cross-sectional VL data yielded consistent results. These findings can serve as a proof of principle that the gap of “missing heritability” in quantitative genetics can be partially bridged by a systematic evaluation of sex-specific associations