14 research outputs found

    Triazole Antifungal Susceptibility Patterns among Aspergillus Species in Quebec, Canada.

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    Contains fulltext : 208939.pdf (publisher's version ) (Closed access)1 juni 201

    Tumour Response 3 Months after Neoadjuvant Single-Fraction Radiotherapy for Low-Risk Breast Cancer

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    Introduction: Standard treatment for early-stage invasive breast cancer (bca) consists of breast-conserving surgery and several weeks of adjuvant radiotherapy (rt). Neoadjuvant single-fraction rt is a novel approach for early-stage bca. We sought to investigate the effect of delaying surgery after neoadjuvant rt with respect to the rate of pathologic response (pr). Methods: Women 65 years of age or older with a new diagnosis of stage i luminal A bca were eligible for inclusion. A single 20 Gy dose to the primary breast tumour was given, followed by breast-conserving surgery 3 months later. The primary endpoint was the pr rate assessed by microscopic evaluation using the Miller–Payne system. Results: To date, 10 patients have been successfully treated. Median age of the patients was 72 years (range: 65–84 years). In 8 patients, neoadjuvant rt resulted in a tumour pr with median residual cellularity of 3%. No immediate rt complications other than mild dermatitis were noted. Conclusions: This study demonstrates a method for delivering single-fraction rt that can lead to a high level of pr in most patients. Continued accrual to this study and subsequent trials are needed to determine the feasibility, safety, and role of this novel technique in the management of early-stage bca

    Functional characterization of two clusters of <em>Brachypodium distachyon</em> UDP-glycosyltransferases encoding putative deoxynivalenol detoxification genes.

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    Plant small molecule UDP-glycosyltransferases (UGT) glycosylate a vast number of endogenous substances but also act in detoxification of metabolites produced by plant pathogenic microorganisms. The ability to inactivate the Fusarium graminearum mycotoxin deoxynivalenol (DON) into DON-3-O-glucoside is crucial for resistance of cereals. We analyzed the UGT gene family of the monocot model species Brachypodium distachyon and functionally characterized two gene clusters containing putative orthologs of previously identified DON-detoxification genes from Arabidopsis thaliana and barley. Analysis of transcription showed that UGTs encoded in both clusters are highly inducible by DON and expressed at much higher levels upon infection with a wild-type DON-producing F. graminearum strain compared to infection with a mutant deficient in DON production. Expression of these genes in a toxin sensitive strain of Saccharomyces cerevisiae revealed that only two B. distachyon UGTs encoded by members of a cluster of six genes homologous to the DON-inactivating barley HvUGT13248 were able to convert DON into DON-3-O-glucoside. Also, a single copy gene from Sorghum bicolor orthologous to this cluster and one of three putative orthologs of rice exhibit this ability. Seemingly, the UGT genes undergo rapid evolution and changes in copy number, making it difficult to identify orthologs with conserved substrate specificity

    Ultrafast Photostriction In Thin Film Bismuth Ferrite And Its Correlation To Electronic Dynamics

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    A series of laser pump, x-ray probe experiments show that above band gap photoexcitation can generate a large out-of-plane strain in multiferroic BiFeO3 thin films. The strain decays in a time scale that is the same as the photo-induced carriers measured in an optical transient absorption spectroscopy experiment. We attribute the strain to the piezoelectric effect due to screening of the depolarization field by laser induced carriers. A strong film thickness dependence of strain and carrier relaxation is also observed, revealing the role of the carrier transport in determining the structural and carrier dynamics in complex oxide thin films. © 2013 Materials Research Society.15282530Catlan, G., Scott, J.F., (2009) Advanced Mat, 21, p. 2463Wang, J., (2003) Science, 299, p. 1719Zhao, T., (2006) Nat Mat, 5, p. 823Lebeugle, D., (2008) Phys. Rev. Lett., 100, p. 227602Nakashima, S., (2009) J. Appl. Phys., 105, p. 061617Wen, H., Phys. Rev. Lett., , in pressKundys, B., (2010) Nat. Mat., 9, p. 803Daranciang, D., (2012) Phys. Rev. Lett., 108, p. 087601Tefft, W.E., (1967) Phys. Rev., 164, p. 1032Sasamoto, S., (2009) J. Appl. Phys., 105, p. 083102Vasiliu-Doloc, L., (1999) Phys. Rev. Lett., 83, p. 4393Fiorentini, V., (1999) Phys. Rev. B, 60, p. 884

    Development of an indirect competitive ELISA for simultaneous detection of enrofloxacin and ciprofloxacin*

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    Modified 1-ethyl-3-(3-dimethylaminopropy) carbodiimide (EDC) method was employed to synthesize the artificial antigen of enrofloxacin (ENR), and New Zealand rabbits were used to produce anti-ENR polyclonal antibody (pAb). Based on the checkerboard titration, an indirect competitive enzyme-linked immunosorbent assay (ELISA) standard curve was established. This assay was sensitive and had a linear range from 0.6 to 148.0 μg/kg (R 2=0.9567), with the half maximal inhibitory concentration (IC50) and limit of detection (LOD) values of 9.4 μg/kg and 0.2 μg/kg, respectively. Of all the competitive analogues, the produced pAb exhibited a high cross-reactivity to ciprofloxacin (CIP) (87%), the main metabolite of ENR in tissues. After optimization, the matrix effects can be ignored using a 10-fold dilution in beef and 20-fold dilution in pork. The overall recoveries and coefficients of variation (CVs) were in the ranges of 86%–109% and 6.8%–13.1%, respectively. It can be concluded that the established ELISA method is suitable for simultaneous detection of ENR and CIP in animal tissues
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