819 research outputs found
Expression of the Wilms' tumor gene WT1 in human malignant mesothelioma cell lines and relationship to platelet-derived growth factor A and insulin- like growth factor 2 expression
Mutations in the WT1 tumor suppressor gene are known to contribute to the development of Wilms' tumor (WT) and associated gonadal abnormalities. WT1 is expressed principally in the fetal kidney, developing gonads, and spleen and also in the mesothelium, which lines the coelomic cavities. These tissues develop from mesenchymal components that have subsequently become epithelialized, and it has therefore been proposed that WT1 may play a role in this transition of cell types. To test the possible involvement of this gene in malignant mesothelioma, we have first studied its expression in a panel of human normal and malignant mesothelial cell lines. WT1 mRNA expression levels varied greatly between the cell lines and no specific chromosomal aberration on 11p, which could be related to the variation in WT1 expression in these cell lines, was observed. Furthermore, no gross deletions, rearrangements, or functionally inactivating point mutations in the WT1 coding region were identified. All four WT1 splice variants were observed at similar levels in these cell lines. The WT1 gene encodes a zinc-finger transcription factor and the four protein isoforms are each believed to act as transcriptional repressors of certain growth factor genes. Lack of WT1 expression is thus predicted to result in growth stimulation of tumor cells. Binding of one particular WT1 isoform construct to the insulin-like growth factor 2 (IGF2) and platelet-derived growth factor A (PDGFA) gene promoters has been demonstrated to result in repression of these genes in transient transfection studies. Analysis of IGF2 and PDGFA mRNA expression levels compared with WT1 mRNA expression levels failed to demonstrate an inverse correlation in the mesothelial cell lines, which endogenously express these genes. Finally, the putative role of WT1 in the transition of cell types was investigated. No obvious correlation between WT1 expression levels and cell morphology of the malignant mesothelial cell lines was evident from this study. Moreover, no change in WT1 expression was observed in normal mesothelial cells which were, by alteration of culture conditions, manipulated to switch from the mesenchymal to epithelial morphology
Computer simulations of hard pear-shaped particles
We report results obtained from Monte Carlo simulations investi-
gating mesophase formation in two model systems of hard pear-shaped
particles. The first model considered is a hard variant of the trun-
cated Stone-Expansion model previously shown to form nematic and
smectic mesophases when embedded within a 12-6 Gay-Berne-like po-
tential [1]. When stripped of its attractive interactions, however, this system is found to lose its liquid crystalline phases. For particles of length to breadth ratio k = 3, glassy behaviour is seen at high pressures, whereas for k = 5 several bi-layer-like domains are seen, with high intradomain order but little interdomain orientational correlation. For the second model, which uses a parametric shape parameter based on the generalised Gay-Berne formalism, results are presented for particles with elongation k = 3; 4 and 5. Here, the systems with k = 3 and 4 fail to display orientationally ordered phases, but that with k = 5 shows isotropic, nematic and, unusually for a hard-particle model, interdigitated smectic A2 phases.</p
Weak decays of : the non-leptonic case
In our previous study, we calculated the transition from factors of
using the QCD sum rules. Based on the factorization
approximation, the obtained form factors can be applied to evaluate the weak
non-leptonic decay rates of , where stands for a
light pseudoscalar or vector meson. We predict that the branching ratio for
inclusive non-leptonic two-body weak decays of which are realized via
the spectator mechanism, can be as large as , in
particular, the branching ratio of can reach
. Such values will be marginally accessed by the ability of
BESIII which will begin running very soon.Comment: 16 pages, revTex4, 1 figur
Larval fish dispersal in a coral-reef seascape
Free to read at publisher's site. Larval dispersal is a critical yet enigmatic process in the persistence and productivity of marine metapopulations. Empirical data on larval dispersal remain scarce, hindering the use of spatial management tools in efforts to sustain ocean biodiversity and fisheries. Here we document dispersal among subpopulations of clownfish (Amphiprion percula) and butterflyfish (Chaetodon vagabundus) from eight sites across a large seascape (10,000 km(2)) in Papua New Guinea across 2 years. Dispersal of clownfish was consistent between years, with mean observed dispersal distances of 15 km and 10 km in 2009 and 2011, respectively. A Laplacian statistical distribution (the dispersal kernel) predicted a mean dispersal distance of 13-19 km, with 90% of settlement occurring within 31-43 km. Mean dispersal distances were considerably greater (43-64 km) for butterfly-fish, with kernels declining only gradually from spawning locations. We demonstrate that dispersal can be measured on spatial scales sufficient to inform the design of and test the performance of marine reserve networks
Functional diversity of chemokines and chemokine receptors in response to viral infection of the central nervous system.
Encounters with neurotropic viruses result in varied outcomes ranging from encephalitis, paralytic poliomyelitis or other serious consequences to relatively benign infection. One of the principal factors that control the outcome of infection is the localized tissue response and subsequent immune response directed against the invading toxic agent. It is the role of the immune system to contain and control the spread of virus infection in the central nervous system (CNS), and paradoxically, this response may also be pathologic. Chemokines are potent proinflammatory molecules whose expression within virally infected tissues is often associated with protection and/or pathology which correlates with migration and accumulation of immune cells. Indeed, studies with a neurotropic murine coronavirus, mouse hepatitis virus (MHV), have provided important insight into the functional roles of chemokines and chemokine receptors in participating in various aspects of host defense as well as disease development within the CNS. This chapter will highlight recent discoveries that have provided insight into the diverse biologic roles of chemokines and their receptors in coordinating immune responses following viral infection of the CNS
Quantum optics in the phase space - A tutorial on Gaussian states
In this tutorial, we introduce the basic concepts and mathematical tools
needed for phase-space description of a very common class of states, whose
phase properties are described by Gaussian Wigner functions: the Gaussian
states. In particular, we address their manipulation, evolution and
characterization in view of their application to quantum information.Comment: Tutorial. 23 pages, 1 figure. Updated version accepted for
publication in EPJ - ST devoted to the memory of Federico Casagrand
Theory and Models of the Disc-Halo Connection
We review the evolution of the interstellar medium in disc galaxies, and
show, both analytically and by numerical 3D hydrodynamic simulations, that the
disc-halo connection is an essential ingredient in understanding the evolution
of star forming galaxies. Depending on the star formation rate of the
underlying gaseous disc, a galactic fountain is established. If the star
formation rate is sufficiently high and/or cosmic rays are well coupled to the
thermal plasma, a galactic wind will be formed and lead to a secular mass loss
of the galaxy. Such a wind leaves a unique imprint on the soft X-ray spectra in
edge-on galaxies, with delayed recombination being one of its distinctive
features. We argue that synthetic spectra, obtained from self-consistent
dynamical and thermal modelling of a galactic outflow, should be treated on an
equal footing as observed spectra. We show that it is thus possible to
successfully fit the spectrum of the starburst galaxy NGC 3079.Comment: 10 pages, 4 figures, Invited review for the proceedings of "The Role
of Disk-Halo Interaction in Galaxy Evolution: Outflow vs Infall?" (Ed. M.A.
de Avillez), in Espinho, Portugal, 18-22 August, 200
Observation of a Coherence Length Effect in Exclusive Rho^0 Electroproduction
Exclusive incoherent electroproduction of the rho^0(770) meson from 1H, 2H,
3He, and 14N targets has been studied by the HERMES experiment at squared
four-momentum transfer Q**2>0.4 GeV**2 and positron energy loss nu from 9 to 20
GeV. The ratio of the 14N to 1H cross sections per nucleon, known as the
nuclear transparency, was found to decrease with increasing coherence length of
quark-antiquark fluctuations of the virtual photon. The data provide clear
evidence of the interaction of the quark- antiquark fluctuations with the
nuclear medium.Comment: RevTeX, 5 pages, 3 figure
Determination of the Deep Inelastic Contribution to the Generalised Gerasimov-Drell-Hearn Integral for the Proton and Neutron
The virtual photon absorption cross section differences [sigma_1/2-sigma_3/2]
for the proton and neutron have been determined from measurements of polarised
cross section asymmetries in deep inelastic scattering of 27.5 GeV
longitudinally polarised positrons from polarised 1H and 3He internal gas
targets. The data were collected in the region above the nucleon resonances in
the kinematic range nu < 23.5 GeV and 0.8 GeV**2 < Q**2 < 12 GeV**2. For the
proton the contribution to the generalised Gerasimov-Drell-Hearn integral was
found to be substantial and must be included for an accurate determination of
the full integral. Furthermore the data are consistent with a QCD
next-to-leading order fit based on previous deep inelastic scattering data.
Therefore higher twist effects do not appear significant.Comment: 6 pages, 3 figures, 1 table, revte
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