A TWO-DIMENSIONAL THERMODYNAMIC MODEL TO PREDICT HEART THERMAL RESPONSE DURING OPEN CHEST PROCEDURES

In this work, the temperature distribution of the heart in an open chest surgery scenario is studied. It is also evaluated the cardiac thermal effects of the injection of a cooling liquid in the aorta root, which is used in infrared thermography. The finite element method was used to develop a model that predicts the temperature distribution modification in a 2-dimensional slice of the heart. This thermodynamic model allows the computational simulation of the thermal cardiac response to open chest procedures, which are required by cardiac surgery. The influence of several operating parameters (e.g., coronary flow rate, temperature) on the resulting thermal distribution is analyzed. Therefore, this analysis allows the identification of parameters that could be controlled to minimize the loss of energy, and consequently, avoiding the hazardous thermal distribution that could put the heart in danger during cardiac surgery

INFRARED IMAGING AND COMPUTERIZED TOMOGRAPHY IN BREAST CANCER: CASE STUDY

This work presents a case study of a 75-year-old woman breast withcancer. The investigation process used infrared image, mammography,computerized tomography (CT) and ultrasound guided biopsy toassess, stage and final diagnostic of the tumor. Each one of theseevaluations brings an isolated piece of information that results in thecorrect diagnostic, and treatment. As early diagnostic of breast cancergoes towards improvement in diagnostics and better therapeutics, it isreasonable to state that breast cancer diagnostics must be achieved asearly as possible. An association between infrared image abnormalitiesand computerized tomography is acknowledged and is assumed that acorrelation could exist. The technical literature demonstrated thattumor depth could be inferred from infrared images, but criticalinformation such as breast perfusion for accurate predictions are notavailable yet. Considering that a mathematical model could modelbreast perfusion, this study proposes that tumor morphology and depthin breast cancer could be adequately determined using mathematicalmodeling, infrared imaging, and computerized tomography incomplementary actions

Star Formation in the Field and Clusters of NGC 5253

We investigate the star formation history of both the bright star clusters and the diffuse `field star' population in the dwarf starburst galaxy NGC 5253 using STIS longslit ultraviolet spectroscopy. Our slit covers a physical area of 370 x 1.6 pc and includes 8 apparent clusters and several inter-cluster regions of diffuse light which we take to be the field. The diffuse light spectrum lacks the strong O-star wind features which are clearly visible in spectra of the brightest clusters. This discrepancy provides compelling evidence that the diffuse light is not reflected light from nearby clusters, but originates in a UV-bright field star population, and it raises the issue of whether the star formation process may be operating differently in the field than in clusters. We compare our spectra to STARBURST99 evolutionary synthesis models which incorporate a new low metallicity atlas of O-star spectra. We favor a scenario which accounts for the paucity of O-stars in the field without requiring the field to have a different IMF than the clusters: stellar clusters form continuously and then dissolve on ~10 Myr timescales and disperse their remaining stars into the field. We consider the probable contribution of an O-star deficient field population to the spatially unresolved spectra of high redshift galaxies. (Abridged)Comment: 26 pages, 10 figures, accepted for publication in Ap

Direct replacement of antibodies with molecularly imprinted polymer (MIP) nanoparticles in ELISA - development of a novel assay for vancomycin

A simple and straightforward technique for coating microplate wells with molecularly imprinted polymer nanoparticles (nanoMIPs) to develop ELISA type assays is presented here for the first time. NanoMIPs were synthesized by a solid phase approach with immobilized vancomycin (template) and characterized using Biacore 3000, dynamic light scattering and electron microscopy. Immobilization, blocking and washing conditions were optimized in microplate format. The detection of vancomycin was achieved in competitive binding experiments with a HRP-vancomycin conjugate. The assay was capable of measuring vancomycin in buffer and in blood plasma within the range 0.001-70 nM with a detection limit of 0.0025 nM (2.5 pM). The sensitivity of the assay was three orders of magnitude better than a previously described ELISA based on antibodies. In these experiments nanoMIPs have shown high affinity and minimal interference from blood plasma components. Immobilized nanoMIPs were stored for 1 month at room temperature without any detrimental effects to their binding properties. The high affinity of nanoMIPs and the lack of a requirement for cold chain logistics make them an attractive alternative to traditional antibodies used in ELIS

SIGMA and XTE observations of the soft X-ray transient XTEJ1755-324

We present observations of the X-ray transient XTEJ1755-324 performed during summer 1997 with the XTE satellite and with the SIGMA hard X-ray telescope onboard the GRANAT observatory. The source was first detected in soft X-rays with XTE on July 25 1997 with a rather soft X-ray spectrum and its outburst was monitored in soft X-rays up to November 1997. On September 16 it was first detected in hard X-rays by the French soft gamma ray telescope SIGMA during a Galactic Center observation. The flux was stronger on September 16 and 17 reaching a level of about 110 mCrab in the 40-80 keV energy band. On the same days the photon index of the spectrum was determined to be alpha =-2.3 +/- 0.9 (1 sigma error) while the 40-150 keV luminosity was about 8 x 10^{36} erg/s for a distance of 8.5 kpc. SIGMA and XTE results on this source indicate that this source had an ultrasoft-like state during its main outburst and a harder secondary outburst in September. These characteristics make the source similar to X-Nova Muscae 1991, a well known black hole candidate.Comment: 19 pages LaTeX, 6 Postscript figures included, Accepted by Astrophysical Journa

A systematic survey of millimetre-wavelength flaring variability of Young Stellar Objects in the Orion Nebula Cluster

High-energy processes are ubiquitous even in the earliest stages of protostellar evolution. Motivated by the results of our systematic search for intense centimeter radio flares in Young Stellar Objects (YSOs) and by rare findings of strong millimeter-wavelength variability, we have conducted a systematic search for such variability in the Orion Nebula Cluster (ONC) using Atacama Large Millimeter/submillimeter Array (ALMA). Rapid variability on timescales of minutes to hours in the (centimeter)millimeter-wavelength range indicates (gyro)synchrotron radiation. Additionally, mass accretion will also affect the millimeter-wavelength luminosity but typically on longer timescales. Beyond studies of individual YSOs, our characterization of strong millimeter-wavelength variability with ALMA in the ONC sets first systematic constraints on the occurrence of such variability in a large number of YSOs ($\sim$130). We report the discovery of an order of magnitude millimeter-flare within just a few minutes from a known YSO previously reported as a radio flaring source at cm-wavelengths (the "ORBS'' source). We also present an assessment of the systematic variability effects caused by the use of time-sliced imaging of a complex region. These are mostly due to the impact of a changing synthesized beam throughout the observations. We use simulated ALMA observations to reproduce and quantify these effects and set a lower limit for the variability that can be studied using our method in a complex region such as the ONC. Our results demonstrate that the utility of time domain analysis of YSOs extends into the millimeter-wavelength range, potentially interfering with the conversion of observed fluxes into dust masses.Comment: 15 pages, 8 figures, accepted for publication in MNRA

Involvement of intact inositol-1,4,5-trisphosphate-sensitive Ca2+ stores in cell cycle progression at the G1/S boundary in serum-stimulated human fibroblasts

AbstractThapsigargin, a selective inhibitor of the endoplasmic reticulum Ca2+ pump, has been shown to deplete inositol-1,4,5-trisphosphate-sensitive Ca2+ stores. Here we report that when thapsigargin was introduced to serum-stimulated human fibroblasts at a time point just before the G1/S boundary, it completely inhibited expression of cyclin A, activation of p33CDK2 cyclindependent kinase and initiation of DNA synthesis. In contrast, the Ca2+ mobilizing ionophore ionomycin was without effect. These findings indicate that Ca2+ inside the inositol-1,4,5-trisphosphatesensitive Ca2+ stores plays a pivotal role for traverse across the G1/S transition point