175 research outputs found
Nonresonant high frequency excitation of mechanical vibrations in graphene based nanoresonator
We theoretically analyse the dynamics of a suspended graphene membrane which
is in tunnel contact with grounded metallic electrodes and subjected to
ac-electrostatic potential induced by a gate electrode. It is shown that for
such system the retardation effects in the electronic subsystem generate an
effective pumping for the relatively slow mechanical vibrations if the driving
frequency exceeds the inverse charge relax- ation time. Under this condition
there is a critical value of the driving voltage ampli- tude above which the
pumping overcomes the intrinsic damping of the mechanical resonator leading to
a mechanical instability. This nonresonant instability is saturated by
nonlinear damping and the system exhibits self-sustained oscillations of
relatively large amplitude.Comment: Major revisio
Dynamics of viscous amphiphilic films supported by elastic solid substrates
The dynamics of amphiphilic films deposited on a solid surface is analyzed
for the case when shear oscillations of the solid surface are excited. The two
cases of surface- and bulk shear waves are studied with film exposed to gas or
to a liquid. By solving the corresponding dispersion equation and the wave
equation while maintaining the energy balance we are able to connect the
surface density and the shear viscocity of a fluid amphiphilic overlayer with
experimentally accessible damping coefficients, phase velocity, dissipation
factor and resonant frequency shifts of shear waves.Comment: 19 pages, latex, 3 figures in eps-forma
Development of a combined surface plasmon resonance/surface acoustic wave device for the characterization of biomolecules
It is known that acoustic sensor devices, if operated in liquid phase, are sensitive not just to the mass of the analyte but also to various other parameters, such as size, shape, charge and elastic constants of the analyte as well as bound and viscously entrained water. This can be used to extract valuable information about a biomolecule, particularly if the acoustic device is combined with another sensor element which is sensitive to the mass or amount of analyte only. The latter is true in good approximation for various optical sensor techniques. This work reports on the development of a combined surface plasmon resonance/surface acoustic wave sensor system which is designed for the investigation of biomolecules such as proteins or DNA. Results for the deposition of neutravidin and DNA are reported
Shuttle Mechanism for Charge Transfer in Coulomb Blockade Nanostructures
Room-temperature Coulomb blockade of charge transport through composite
nanostructures containing organic inter-links has recently been observed. A
pronounced charging effect in combination with the softness of the molecular
links implies that charge transfer gives rise to a significant deformation of
these structures. For a simple model system containing one nanoscale metallic
cluster connected by molecular links to two bulk metallic electrodes we show
that self-excitation of periodic cluster oscillations in conjunction with
sequential processes of cluster charging and decharging appears for a
sufficiently large bias voltage. This new `electron shuttle' mechanism of
discrete charge transfer gives rise to a current through the nanostructure,
which is proportional to the cluster vibration frequency.Comment: 4 pages, 4 figure
ΠΠΎΠ²ΡΠΉ Π²ΡΡΠΎΠΊΠΎΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΡΠΉ ΡΡΠ°ΠΌΠΌ Propionibacterium acidipropionici FL-48 Ρ ΠΏΠΎΠ²ΡΡΠ΅Π½Π½ΠΎΠΉ ΡΡΡΠΎΠΉΡΠΈΠ²ΠΎΡΡΡΡ ΠΊ ΠΏΡΠΎΠΏΠΈΠΎΠ½ΠΎΠ²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΠ΅ ΠΈ ΠΌΠ°ΡΡΡΠ°Π±ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ΅Ρ Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈ Π΅Π³ΠΎ Π½Π°ΡΠ°Π±ΠΎΡΠΊΠΈ Π² ΠΏΡΠΎΠΌΡΡΠ»Π΅Π½Π½ΡΡ Π±ΠΈΠΎΡΠ΅Π°ΠΊΡΠΎΡΠ°Ρ
Propionic acid bacteria, includingΒ Propionibacterium acidipropionici, are widely used in the chemical industry to produce propionic acid and also for food and feed preservation. However, the efficiency of the industrial production of these bacteria is limited by their sensitivity to high concentrations of propionic acid excreted into the cultivation medium. Therefore, the development of new biotechnological processes and strains able to overcome this limitation and to improve the profitability of the microbiological production remainsΒ a relevant problem. AΒ newΒ P. acidipropioniciΒ FL-48 strain characterized by an increased resistance to 10 g/L of propionic acid (the number of viable cells after 24-h cultivation reached 1.05 Γ 106) was developed by a two-step induced mutagenesis using UV and diethyl sulphate from theΒ P.Β acidipropioniciΒ VKPM B-5723 strain. The mutant strain exceeded the parental strain in the biomass accumulation rate and the amount of produced propionic and acetic acids by 35%, 20%, and 16%, respectively. The stability of such important characteristics as the biomass accumulation rate and the viability on media containing heightened concentrations of propionic acid was confirmed by three sequential monoclonal subculturings on a medium supplemented with 10 g/L of propionic acid. The optimization of the cultivation technology made it possible to determine the optimum seed inoculum dose (10% of the fermentation medium volume) and the best pH level for the active growth stage (6.1 Β± 0.1). The scaling up of the fermentation to a 100-L bioreactor under observance of optimum cultivation conditions demonstrated a high biomass growth rate with a sufficient reproducability; after 20 h of fermentation, the number of viable cells in the culture broth exceeded 1 Γ 1010Β CFU/mL. The new strain could be interesting as the component of silage and haylage biopreservatives and also could be used as an efficient producer of propionic acid.ΠΡΠΎΠΏΠΈΠΎΠ½ΠΎΠ²ΠΎΠΊΠΈΡΠ»ΡΠ΅ Π±Π°ΠΊΡΠ΅ΡΠΈΠΈ, Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ Propionibacterium acidipropionici, ΡΠΈΡΠΎΠΊΠΎ ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΡΡΡΡ Π² Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΏΡΠΎΠΌΡΡΠ»Π΅Π½Π½ΠΎΡΡΠΈ Π΄Π»Ρ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΠΏΡΠΎΠΏΠΈΠΎΠ½ΠΎΠ²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ, Π° ΡΠ°ΠΊΠΆΠ΅ Π΄Π»Ρ ΠΊΠΎΠ½ΡΠ΅ΡΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΈΡΠΈ ΠΈ Π·Π°Π³ΠΎΡΠΎΠ²ΠΊΠΈ Π·Π΅ΡΠ½Π° ΠΈ Π·Π΅Π»Π΅Π½ΡΡ
ΠΊΠΎΡΠΌΠΎΠ². ΠΠ΄Π½Π°ΠΊΠΎ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΏΡΠΎΠΌΡΡΠ»Π΅Π½Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΡΡΠ²Π° Π±ΠΈΠΎΠΌΠ°ΡΡΡ ΠΏΡΠΎΠΏΠΈΠΎΠ½ΠΎΠ²ΠΎΠΊΠΈΡΠ»ΡΡ
Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ ΠΎΠ³ΡΠ°Π½ΠΈΡΠ΅Π½Π° ΠΈΡ
ΡΡΠ²ΡΡΠ²ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΡΡ ΠΊ Π²ΡΡΠΎΠΊΠΈΠΌ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΡΠΌ Π² ΡΡΠ΅Π΄Π΅ ΠΏΡΠΎΠΏΠΈΠΎΠ½ΠΎΠ²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ. Π’Π°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ, Π°ΠΊΡΡΠ°Π»ΡΠ½ΠΎΠΉ Π·Π°Π΄Π°ΡΠ΅ΠΉ ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠ° Π½ΠΎΠ²ΡΡ
Π±ΠΈΠΎΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² ΠΈ ΡΡΠ°ΠΌΠΌΠΎΠ², ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡΠΈΡ
ΠΏΡΠ΅ΠΎΠ΄ΠΎΠ»Π΅ΡΡ ΡΡΠΎ ΠΎΠ³ΡΠ°Π½ΠΈΡΠ΅Π½ΠΈΠ΅ ΠΈ ΠΏΠΎΠ²ΡΡΠΈΡΡ ΡΠ΅Π½ΡΠ°Π±Π΅Π»ΡΠ½ΠΎΡΡΡ ΠΌΠΈΠΊΡΠΎΠ±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΡΡΠ²Π°. ΠΠ΅ΡΠΎΠ΄ΠΎΠΌ Π΄Π²ΡΡ
ΡΡΡΠΏΠ΅Π½ΡΠ°ΡΠΎΠ³ΠΎ ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ΠΌΡΡΠ°Π³Π΅Π½Π΅Π·Π° Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ Π£Π€-ΠΎΠ±Π»ΡΡΠ΅Π½ΠΈΡ ΠΈ Π΄ΠΈΡΡΠΈΠ»ΡΡΠ»ΡΡΠ°ΡΠ° ΠΏΠΎΠ»ΡΡΠ΅Π½ Π½ΠΎΠ²ΡΠΉ ΠΌΡΡΠ°Π½ΡΠ½ΡΠΉ ΡΡΠ°ΠΌΠΌ P. acidipropionici Π€Π-48, ΠΎΠ±Π»Π°Π΄Π°ΡΡΠΈΠΉ ΠΏΠΎΠ²ΡΡΠ΅Π½Π½ΠΎΠΉ ΡΠ΅Π·ΠΈΡΡΠ΅Π½ΡΠ½ΠΎΡΡΡΡ ΠΊ 10 Π³/Π» ΠΏΡΠΎΠΏΠΈΠΎΠ½ΠΎΠ²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ (ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ ΠΆΠΈΠ·Π½Π΅ΡΠΏΠΎΡΠΎΠ±Π½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ΅ΡΠ΅Π· 24 Ρ ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π΄ΠΎΡΡΠΈΠ³Π°Π»ΠΎ 1,05 Γ 106) ΠΈ ΠΏΡΠ΅Π²ΠΎΡΡ
ΠΎΠ΄ΡΡΠΈΠΉ ΡΠΎΠ΄ΠΈΡΠ΅Π»ΡΡΠΊΠΈΠΉ ΡΡΠ°ΠΌΠΌ P.Β acidipropionici ΠΠΠΠ Π-5723 ΠΏΠΎ ΡΠΊΠΎΡΠΎΡΡΠΈ Π½Π°ΠΊΠΎΠΏΠ»Π΅Π½ΠΈΡ Π±ΠΈΠΎΠΌΠ°ΡΡΡ ΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Ρ ΠΏΡΠΎΠ΄ΡΡΠΈΡΡΠ΅ΠΌΡΡ
ΠΏΡΠΎΠΏΠΈΠΎΠ½ΠΎΠ²ΠΎΠΉ ΠΈ ΡΠΊΡΡΡΠ½ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡ Π½Π° 35%, 20% ΠΈ 16%, ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ. Π‘ΡΠ°Π±ΠΈΠ»ΡΠ½ΠΎΡΡΡ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊ Π½ΠΎΠ²ΠΎΠ³ΠΎ ΡΡΠ°ΠΌΠΌΠ° (ΡΠΊΠΎΡΠΎΡΡΡ Π½Π°ΠΊΠΎΠΏΠ»Π΅Π½ΠΈΡ Π±ΠΈΠΎΠΌΠ°ΡΡΡ ΠΈ ΠΆΠΈΠ·Π½Π΅ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡ Π½Π° ΡΡΠ΅Π΄Π°Ρ
Ρ ΠΏΠΎΠ²ΡΡΠ΅Π½Π½ΠΎΠΉ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠ΅ΠΉ ΠΏΡΠΎΠΏΠΈΠΎΠ½ΠΎΠ²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ) ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π΅Π½Π° ΡΡΠ΅Ρ
ΠΊΡΠ°ΡΠ½ΡΠΌ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΡΠΌ ΠΌΠΎΠ½ΠΎΠΊΠ»ΠΎΠ½Π°Π»ΡΠ½ΡΠΌ ΡΠ°ΡΡΠ΅Π²ΠΎΠΌ Π½Π° ΡΡΠ΅Π΄Ρ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΡΡ 10 Π³/Π» ΠΏΡΠΎΠΏΠΈΠΎΠ½ΠΎΠ²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ. ΠΡΠΏΠΎΠ»Π½Π΅Π½Π½Π°Ρ ΠΎΠΏΡΠΈΠΌΠΈΠ·Π°ΡΠΈΡ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΡΠ°ΠΌΠΌΠ° ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ»Π° ΠΎΠΏΡΠ΅Π΄Π΅Π»ΠΈΡΡ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΡΡ Π΄ΠΎΠ·Ρ ΠΈΠ½ΠΎΠΊΡΠ»ΡΠΌΠ° Π΄Π»Ρ Π·Π°ΡΠ΅Π²Π° Π±ΠΈΠΎΡΠ΅Π°ΠΊΡΠΎΡΠ° (10% ΠΎΡ ΠΎΠ±ΡΠ΅ΠΌΠ° ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΡΠ΅Π΄Ρ) ΠΈ ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠΈΠ²Π°Π΅ΠΌΡΠΉ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΏΠ΅ΡΠ²ΡΡ
12 Ρ ΡΡΠΎΠ²Π΅Π½Ρ ΡΠ ΡΡΠ΅Π΄Ρ, ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΠ²Π°ΡΡΠΈΠΉ ΠΌΠ°ΠΊΡΠΈΠΌΠ°Π»ΡΠ½ΡΠΉ ΠΏΡΠΈΡΠΎΡΡ Π±ΠΈΠΎΠΌΠ°ΡΡΡ (6,1 Β± 0,1). ΠΡΠΎΠ²Π΅Π΄Π΅Π½Π½ΠΎΠ΅ ΠΌΠ°ΡΡΡΠ°Π±ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°ΡΠΈΠΈ Π΄ΠΎ 100-Π»ΠΈΡΡΠΎΠ²ΠΎΠ³ΠΎ Π±ΠΈΠΎΡΠ΅Π°ΠΊΡΠΎΡΠ° Ρ ΡΠΎΠ±Π»ΡΠ΄Π΅Π½ΠΈΠ΅ΠΌ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΡΡ
ΡΡΠ»ΠΎΠ²ΠΈΠΉ ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΎ ΡΠΎΡ
ΡΠ°Π½Π΅Π½ΠΈΠ΅ Π²ΡΡΠΎΠΊΠΎΠΉ ΡΠΊΠΎΡΠΎΡΡΠΈ ΡΠΎΡΡΠ° ΡΡΠ°ΠΌΠΌΠ° Π² ΡΡΠ»ΠΎΠ²ΠΈΡΡ
ΠΏΠΎΠ½ΠΈΠΆΠ΅Π½Π½ΠΎΠ³ΠΎ ΡΠ; ΡΠΆΠ΅ ΠΊ 20-ΠΌΡ ΡΠ°ΡΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°ΡΠΈΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ ΠΆΠΈΠ·Π½Π΅ΡΠΏΠΎΡΠΎΠ±Π½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ Π² ΠΊΡΠ»ΡΡΡΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠΈ ΠΏΡΠ΅Π²ΡΡΠ°Π»ΠΎ 1 Γ 1010 ΠΠΠ/ΠΌΠ». ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ Ρ
ΠΎΡΠΎΡΡΡ Π²ΠΎΡΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΠΈΠΌΠΎΡΡΡ. ΠΠΎΠ²ΡΠΉ ΡΡΠ°ΠΌΠΌ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅Ρ ΠΈΠ½ΡΠ΅ΡΠ΅Ρ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠ° Π±ΠΈΠΎΠΊΠΎΠ½ΡΠ΅ΡΠ²Π°Π½ΡΠΎΠ² Π΄Π»Ρ ΡΠΈΠ»ΠΎΡΠ° ΠΈ ΡΠ΅Π½Π°ΠΆΠ°, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΠ΄ΡΡΠ΅Π½ΡΠ° ΠΏΡΠΎΠΏΠΈΠΎΠ½ΠΎΠ²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ.Β ΠΡΠΎΠΏΠΈΠΎΠ½ΠΎΠ²ΠΎΠΊΠΈΡΠ»ΡΠ΅ Π±Π°ΠΊΡΠ΅ΡΠΈΠΈ, Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ Propionibacterium acidipropionici, ΡΠΈΡΠΎΠΊΠΎ ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΡΡΡΡ Π² Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΏΡΠΎΠΌΡΡΠ»Π΅Π½Π½ΠΎΡΡΠΈ Π΄Π»Ρ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΠΏΡΠΎΠΏΠΈΠΎΠ½ΠΎΠ²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ, Π° ΡΠ°ΠΊΠΆΠ΅ Π΄Π»Ρ ΠΊΠΎΠ½ΡΠ΅ΡΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΈΡΠΈ ΠΈ Π·Π°Π³ΠΎΡΠΎΠ²ΠΊΠΈ Π·Π΅ΡΠ½Π° ΠΈ Π·Π΅Π»Π΅Π½ΡΡ
ΠΊΠΎΡΠΌΠΎΠ². ΠΠ΄Π½Π°ΠΊΠΎ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΏΡΠΎΠΌΡΡΠ»Π΅Π½Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΡΡΠ²Π° Π±ΠΈΠΎΠΌΠ°ΡΡΡ ΠΏΡΠΎΠΏΠΈΠΎΠ½ΠΎΠ²ΠΎΠΊΠΈΡΠ»ΡΡ
Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ ΠΎΠ³ΡΠ°Π½ΠΈΡΠ΅Π½Π° ΠΈΡ
ΡΡΠ²ΡΡΠ²ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΡΡ ΠΊ Π²ΡΡΠΎΠΊΠΈΠΌ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΡΠΌ Π² ΡΡΠ΅Π΄Π΅ ΠΏΡΠΎΠΏΠΈΠΎΠ½ΠΎΠ²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ. Π’Π°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ, Π°ΠΊΡΡΠ°Π»ΡΠ½ΠΎΠΉ Π·Π°Π΄Π°ΡΠ΅ΠΉ ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠ° Π½ΠΎΠ²ΡΡ
Π±ΠΈΠΎΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² ΠΈ ΡΡΠ°ΠΌΠΌΠΎΠ², ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡΠΈΡ
ΠΏΡΠ΅ΠΎΠ΄ΠΎΠ»Π΅ΡΡ ΡΡΠΎ ΠΎΠ³ΡΠ°Π½ΠΈΡΠ΅Π½ΠΈΠ΅ ΠΈ ΠΏΠΎΠ²ΡΡΠΈΡΡ ΡΠ΅Π½ΡΠ°Π±Π΅Π»ΡΠ½ΠΎΡΡΡ ΠΌΠΈΠΊΡΠΎΠ±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΡΡΠ²Π°. ΠΠ΅ΡΠΎΠ΄ΠΎΠΌ Π΄Π²ΡΡ
ΡΡΡΠΏΠ΅Π½ΡΠ°ΡΠΎΠ³ΠΎ ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ΠΌΡΡΠ°Π³Π΅Π½Π΅Π·Π° Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ Π£Π€-ΠΎΠ±Π»ΡΡΠ΅Π½ΠΈΡ ΠΈ Π΄ΠΈΡΡΠΈΠ»ΡΡΠ»ΡΡΠ°ΡΠ° ΠΏΠΎΠ»ΡΡΠ΅Π½ Π½ΠΎΠ²ΡΠΉ ΠΌΡΡΠ°Π½ΡΠ½ΡΠΉ ΡΡΠ°ΠΌΠΌ P. acidipropionici Π€Π-48, ΠΎΠ±Π»Π°Π΄Π°ΡΡΠΈΠΉ ΠΏΠΎΠ²ΡΡΠ΅Π½Π½ΠΎΠΉ ΡΠ΅Π·ΠΈΡΡΠ΅Π½ΡΠ½ΠΎΡΡΡΡ ΠΊ 10 Π³/Π» ΠΏΡΠΎΠΏΠΈΠΎΠ½ΠΎΠ²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ (ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ ΠΆΠΈΠ·Π½Π΅ΡΠΏΠΎΡΠΎΠ±Π½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ΅ΡΠ΅Π· 24 Ρ ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π΄ΠΎΡΡΠΈΠ³Π°Π»ΠΎ 1,05 Γ 106) ΠΈ ΠΏΡΠ΅Π²ΠΎΡΡ
ΠΎΠ΄ΡΡΠΈΠΉ ΡΠΎΠ΄ΠΈΡΠ΅Π»ΡΡΠΊΠΈΠΉ ΡΡΠ°ΠΌΠΌ P.Β acidipropionici ΠΠΠΠ Π-5723 ΠΏΠΎ ΡΠΊΠΎΡΠΎΡΡΠΈ Π½Π°ΠΊΠΎΠΏΠ»Π΅Π½ΠΈΡ Π±ΠΈΠΎΠΌΠ°ΡΡΡ ΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Ρ ΠΏΡΠΎΠ΄ΡΡΠΈΡΡΠ΅ΠΌΡΡ
ΠΏΡΠΎΠΏΠΈΠΎΠ½ΠΎΠ²ΠΎΠΉ ΠΈ ΡΠΊΡΡΡΠ½ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡ Π½Π° 35%, 20% ΠΈ 16%, ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ. Π‘ΡΠ°Π±ΠΈΠ»ΡΠ½ΠΎΡΡΡ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊ Π½ΠΎΠ²ΠΎΠ³ΠΎ ΡΡΠ°ΠΌΠΌΠ° (ΡΠΊΠΎΡΠΎΡΡΡ Π½Π°ΠΊΠΎΠΏΠ»Π΅Π½ΠΈΡ Π±ΠΈΠΎΠΌΠ°ΡΡΡ ΠΈ ΠΆΠΈΠ·Π½Π΅ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡ Π½Π° ΡΡΠ΅Π΄Π°Ρ
Ρ ΠΏΠΎΠ²ΡΡΠ΅Π½Π½ΠΎΠΉ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠ΅ΠΉ ΠΏΡΠΎΠΏΠΈΠΎΠ½ΠΎΠ²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ) ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π΅Π½Π° ΡΡΠ΅Ρ
ΠΊΡΠ°ΡΠ½ΡΠΌ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΡΠΌ ΠΌΠΎΠ½ΠΎΠΊΠ»ΠΎΠ½Π°Π»ΡΠ½ΡΠΌ ΡΠ°ΡΡΠ΅Π²ΠΎΠΌ Π½Π° ΡΡΠ΅Π΄Ρ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΡΡ 10 Π³/Π» ΠΏΡΠΎΠΏΠΈΠΎΠ½ΠΎΠ²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ. ΠΡΠΏΠΎΠ»Π½Π΅Π½Π½Π°Ρ ΠΎΠΏΡΠΈΠΌΠΈΠ·Π°ΡΠΈΡ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΡΠ°ΠΌΠΌΠ° ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ»Π° ΠΎΠΏΡΠ΅Π΄Π΅Π»ΠΈΡΡ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΡΡ Π΄ΠΎΠ·Ρ ΠΈΠ½ΠΎΠΊΡΠ»ΡΠΌΠ° Π΄Π»Ρ Π·Π°ΡΠ΅Π²Π° Π±ΠΈΠΎΡΠ΅Π°ΠΊΡΠΎΡΠ° (10% ΠΎΡ ΠΎΠ±ΡΠ΅ΠΌΠ° ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΡΠ΅Π΄Ρ) ΠΈ ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠΈΠ²Π°Π΅ΠΌΡΠΉ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΏΠ΅ΡΠ²ΡΡ
12 Ρ ΡΡΠΎΠ²Π΅Π½Ρ ΡΠ ΡΡΠ΅Π΄Ρ, ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΠ²Π°ΡΡΠΈΠΉ ΠΌΠ°ΠΊΡΠΈΠΌΠ°Π»ΡΠ½ΡΠΉ ΠΏΡΠΈΡΠΎΡΡ Π±ΠΈΠΎΠΌΠ°ΡΡΡ (6,1 Β± 0,1). ΠΡΠΎΠ²Π΅Π΄Π΅Π½Π½ΠΎΠ΅ ΠΌΠ°ΡΡΡΠ°Π±ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°ΡΠΈΠΈ Π΄ΠΎ 100-Π»ΠΈΡΡΠΎΠ²ΠΎΠ³ΠΎ Π±ΠΈΠΎΡΠ΅Π°ΠΊΡΠΎΡΠ° Ρ ΡΠΎΠ±Π»ΡΠ΄Π΅Π½ΠΈΠ΅ΠΌ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΡΡ
ΡΡΠ»ΠΎΠ²ΠΈΠΉ ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΎ ΡΠΎΡ
ΡΠ°Π½Π΅Π½ΠΈΠ΅ Π²ΡΡΠΎΠΊΠΎΠΉ ΡΠΊΠΎΡΠΎΡΡΠΈ ΡΠΎΡΡΠ° ΡΡΠ°ΠΌΠΌΠ° Π² ΡΡΠ»ΠΎΠ²ΠΈΡΡ
ΠΏΠΎΠ½ΠΈΠΆΠ΅Π½Π½ΠΎΠ³ΠΎ ΡΠ; ΡΠΆΠ΅ ΠΊ 20-ΠΌΡ ΡΠ°ΡΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°ΡΠΈΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ ΠΆΠΈΠ·Π½Π΅ΡΠΏΠΎΡΠΎΠ±Π½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ Π² ΠΊΡΠ»ΡΡΡΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠΈ ΠΏΡΠ΅Π²ΡΡΠ°Π»ΠΎ 1 Γ 1010 ΠΠΠ/ΠΌΠ». ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ Ρ
ΠΎΡΠΎΡΡΡ Π²ΠΎΡΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΠΈΠΌΠΎΡΡΡ. ΠΠΎΠ²ΡΠΉ ΡΡΠ°ΠΌΠΌ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅Ρ ΠΈΠ½ΡΠ΅ΡΠ΅Ρ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠ° Π±ΠΈΠΎΠΊΠΎΠ½ΡΠ΅ΡΠ²Π°Π½ΡΠΎΠ² Π΄Π»Ρ ΡΠΈΠ»ΠΎΡΠ° ΠΈ ΡΠ΅Π½Π°ΠΆΠ°, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΠ΄ΡΡΠ΅Π½ΡΠ° ΠΏΡΠΎΠΏΠΈΠΎΠ½ΠΎΠ²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ.
Outcomes of ROHs (runs of homozygosity)/ LCSHs (long contiguous stretches of homozygosity) spanning the imprinted loci of chromosomes 7, 11 and 15 among children with neurodevelopmental disorders
To describe the outcomes of ROHs/LCSHs spanning the imprinted loci of chromosomes 7, 11 and 15 among children with neurodevelopmental disorder
Electrospun magnetic composite poly-3-hydroxybutyrate/magnetite scaffolds for biomedical applications: composition, structure, magnetic properties, and biological performance
Magnetically responsive composite polymer scaffolds have good potential for a variety of biomedical applications. In this work, electrospun composite scaffolds made of polyhydroxybutyrate (PHB) and magnetite (Fe3O4) particles (MPs) were studied before and after degradation in either PBS or a lipase solution. MPs of different sizes with high saturation magnetization were synthesized by the coprecipitation method followed by coating with citric acid (CA). Nanosized MPs were prone to magnetite-maghemite phase transformation during scaffold fabrication, as revealed by Raman spectroscopy; however, for CA-functionalized nanoparticles, the main phase was found to be magnetite, with some traces of maghemite. Submicron MPs were resistant to the magnetite-maghemite phase transformation. MPs did not significantly affect the morphology and diameter of PHB fibers. The scaffolds containing CA-coated MPs lost 0.3 or 0.2% of mass in the lipase solution and PBS, respectively, whereas scaffolds doped with unmodified MPs showed no mass changes after 1 month of incubation in either medium. In all electrospun scaffolds, no alterations of the fiber morphology were observed. Possible mechanisms of the crystalline-lamellar-structure changes in hybrid PHB/Fe3O4 scaffolds during hydrolytic and enzymatic degradation are proposed. It was revealed that particle size and particle surface functionalization affect the mechanical properties of the hybrid scaffolds. The addition of unmodified MPs increased scaffolds' ultimate strength but reduced elongation at break after the biodegradation, whereas simultaneous increases in both parameters were observed for composite scaffolds doped with CA-coated MPs. The highest saturation magnetization-higher than that published in the literature-was registered for composite PHB scaffolds doped with submicron MPs. All PHB scaffolds proved to be biocompatible, and the ones doped with nanosized MPs yielded faster proliferation of rat mesenchymal stem cells. In addition, all electrospun scaffolds were able to support angiogenesis in vivo at 30 days after implantation in Wistar rats
Role of Caustic Addition in Bitumen-Clay Interactions
Coating of bitumen by clays, known as slime coating, is detrimental to bitumen recovery from oil sands using the warm slurry extn. process. Sodium hydroxide (caustic) is added to the extn. process to balance many competing processing challenges, which include undesirable slime coating. The current research aims at understanding the role of caustic addn. in controlling interactions of bitumen with various types of model clays. The interaction potential was studied by quartz crystal microbalance with dissipation monitoring (QCM-D). After confirming the slime coating potential of montmorillonite clays on bitumen in the presence of calcium ions, the interaction of kaolinite and illite with bitumen was studied. To represent more closely the industrial applications, tailings water from bitumen extn. tests at different caustic dosage was used. At caustic dosage up to 0.5 wt % oil sands ore, a negligible coating of kaolinite on the bitumen was detd. However, at a lower level of caustic addn., illite was shown to attach to the bitumen, with the interaction potential decreasing with increasing caustic dosage. Increasing concn. of humic acids as a result of increasing caustic dosage was identified to limit the interaction potential of illite with bitumen. This fundamental study clearly shows that the crit. role of caustics in modulating interactions of clays with bitumen depends upon the type of clays. Thus, clay type was identified as a key operational variable
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