93 research outputs found
The quality of medical care in a round-the-clock hospital during the COVID-19 pandemic
Violations in the provision of medical care are detected by controlling the volume, timing, quality and conditions of medical care. The objective of the work is assessment of the activities of the round-the-clock hospital from the standpoint of the quality of medical care during the COVID-19 pandemic. Material and methods. A database of indicators reο¬ecting the activity of round-the-clock hospital for four years, from 2017 to 2020 (Omsk) was formed. Analytical, statistical methods and the method of expert assessments were used to analyze the required indicators. Results. Evaluation of inpatient hospital activities during the COVID-19 pandemic compared to the βpre-pandemicβ years showed a 1.9-fold and 2.9-fold decrease in the total number of quality examinations performed and the number of defects detected, respectively. The number of defects that were grounds for denial or reduction of payment for medical care found in the βbefore-COVID-19β years was 2.1 times higher than in 2020. In 2020, the weight of the share of those defects that directly reο¬ect the quality of medical care increased signifcantly. The quantitative composition of the inpatient hospital staο¬ changed insignifcantly during the analyzed period β the number of physicians increased Β by only 5 % over four years, with no dynamics in the qualitative characteristics of the staο¬. The number of medical equipment increased by 27.6 %. Due to re-profling in 2020, there was a decrease in the number of hospitalized patients, with an increase in the proportion of patients admitted by emergency care (up to 91 %) and an increase in mortality by 2.7 times. Conclusions. One of the conditions for maintaining high quality of medical care is a balance between the main components of quality: accessibility, timeliness, sufciency, efciency and safety and the speed of its achievement, which in turn depends on the amount of resources and reserves
Insilico test of functional role of rs8068318 polymorphism of arterial hypertension-associated TBX2 candidate gene
In the course of this study, the functional role of rs8068318 polymorphism of the TBX2
candidate gene associated with the development of arterial hypertension was studied. The selection of the polymorphic locus was based on the data of the catalog of genome-wide association study (GWAS) of the National Human Genome Research Institute. The
functional role was evaluated using online software: HaploReg (v4.1), GTExportal, and PolyPhen-
Agent-based modeling of the impact of advertising on the regional economic cluster lifecycle
The aim of the study is the development and testing of an algorithm for modeling the impact of advertising on various stages of the life cycle of economic clusters. It is assumed, that the life cycle of the cluster consists of the stages: a diffuse group, a hidden cluster, an evolving cluster, a mature cluster, a collapsing cluster. Using the agent-based simulation methods, hierarchical clustering and chaos theory, the following results were obtained: a conceptual model of the behavior of cluster members for cluster formation processes at each stage of the cluster life cycle and an imitation model of the influence of advertising on the life cycle of the economic cluster; the patterns of various stages of the life cycle of the economic cluster and the functioning of the cluster without influence and under the influence of advertising were revealed. Advertising reduces the time at the stages of the associated life cycle of the cluster, increases the stage of maturity of the cluster. Companies that do not comply with the principles of clustering are under the influence of advertising and promotional activities. Such enterprises most often arise in the cluster at the stages of its formation
Π’Π΅ΡΠ΅Π½ΠΈΠ΅ ΠΌΡΠΊΠΎΠ²ΠΈΡΡΠΈΠ΄ΠΎΠ·Π° Ρ Π²Π·ΡΠΎΡΠ»ΠΎΠ³ΠΎ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠ° Ρ "ΡΡΠΆΠ΅Π»ΡΠΌ" Π³Π΅Π½ΠΎΡΠΈΠΏΠΎΠΌ
Cystic fibrosis (CF) is the commonest monogenic autosomal recessive disease. CF pathogenesis is based on systemic injury of exocrine glands due to CFTR gene mutation. CF case in an adult patient with "severe" genotype and multiple complications, such as chronic hypercapnic respiratory failure, diabetes mellitus, hepatic cirrhosis, cholelithiasis, pulmonary hypertension, and an episode of bowel obstruction, is described in the article. Despite of a number of complications this case demonstrated good efficacy of home respiratory support during 2.5 years. Novel methods of respiratory support, supplemental oxygen and intensive basic therapy have allowed stabilization his clinical status.ΠΡΠΊΠΎΠ²ΠΈΡΡΠΈΠ΄ΠΎΠ· (ΠΠ) β ΡΠ°ΠΌΠΎΠ΅ ΡΠ°ΡΡΠΎΠ΅ ΠΌΠΎΠ½ΠΎΠ³Π΅Π½Π½ΠΎΠ΅ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠ΅ Ρ Π°ΡΡΠΎΡΠΎΠΌΠ½ΠΎ-ΡΠ΅ΡΠ΅ΡΡΠΈΠ²Π½ΡΠΌ ΡΠΈΠΏΠΎΠΌ Π½Π°ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ, Π² ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠ°ΡΠΎΠ³Π΅Π½Π΅Π·Π° ΠΊΠΎΡΠΎΡΠΎΠ³ΠΎ Π»Π΅ΠΆΠΈΡ ΡΠΈΡΡΠ΅ΠΌΠ½Π°Ρ ΡΠΊΠ·ΠΎΠΊΡΠΈΠ½ΠΎΠΏΠ°ΡΠΈΡ, Π²ΡΠ·Π²Π°Π½Π½Π°Ρ ΠΌΡΡΠ°ΡΠΈΠ΅ΠΈΜ ΠΈ ΠΏΠΎΡΠ»Π΅Π΄ΡΡΡΠ΅ΠΈΜ Π΄ΠΈΡΡΡΠ½ΠΊΡΠΈΠ΅ΠΈΜ Π³Π΅Π½Π° ΠΈ Π±Π΅Π»ΠΊΠ° ΡΡΠ°Π½ΡΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π½ΠΎΠ³ΠΎ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ° ΠΠ. ΠΡΠΎΠ΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΠΎΠ²Π°Π½ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΠΈΜ ΡΠ»ΡΡΠ°ΠΈΜ ΡΠ΅ΡΠ΅Π½ΠΈΡ ΠΠ Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠ° Ρ "ΡΡΠΆΠ΅Π»ΡΠΌ" Π³Π΅Π½ΠΎΡΠΈΠΏΠΎΠΌ Π²ΠΎ Π²Π·ΡΠΎΡΠ»ΠΎΠΌ Π²ΠΎΠ·ΡΠ°ΡΡΠ΅ ΠΈ ΠΌΠ½ΠΎΠ³ΠΎΡΠΈΡΠ»Π΅Π½Π½ΡΠΌΠΈ ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΡΠΌΠΈ (Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠ°Ρ Π³ΠΈΠΏΠ΅ΡΠΊΠ°ΠΏΠ½ΠΈΡΠ΅ΡΠΊΠ°Ρ Π΄ΡΡ
Π°ΡΠ΅Π»ΡΠ½Π°Ρ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎΡΡΡ, ΡΠ°Ρ
Π°ΡΠ½ΡΠΈΜ Π΄ΠΈΠ°Π±Π΅Ρ, ΡΠΈΡΡΠΎΠ· ΠΏΠ΅ΡΠ΅Π½ΠΈ, ΠΆΠ΅Π»ΡΠ½ΠΎΠΊΠ°ΠΌΠ΅Π½Π½Π°Ρ Π±ΠΎΠ»Π΅Π·Π½Ρ, Π»Π΅Π³ΠΎΡΠ½Π°Ρ Π³ΠΈΠΏΠ΅ΡΡΠ΅Π½Π·ΠΈΡ, ΡΠΏΠΈΠ·ΠΎΠ΄ ΠΊΠΈΡΠ΅ΡΠ½ΠΎΠΈΜ Π½Π΅ΠΏΡΠΎΡ
ΠΎΠ΄ΠΈΠΌΠΎΡΡΠΈ). ΠΠ΅ΡΠΌΠΎΡΡΡ Π½Π° Π±ΠΎΠ»ΡΡΠΎΠ΅ ΡΠΈΡΠ»ΠΎ ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠΈΜ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ, ΠΏΡΠΎΠ΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΠΎΠ²Π°Π½Π° Π²ΡΡΠΎΠΊΠ°Ρ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π°ΠΌΠ±ΡΠ»Π°ΡΠΎΡΠ½ΠΎΠΈΜ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎΠΈΜ ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠΊΠΈ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 2,5 Π³ΠΎΠ΄Π°. Π‘ ΠΏΠΎΠΌΠΎΡΡΡ ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎΠΈΜ ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠΊΠΈ ΠΈ ΠΊΠΈΡΠ»ΠΎΡΠΎΠ΄ΠΎΡΠ΅ΡΠ°ΠΏΠΈΠΈ, Π° ΡΠ°ΠΊΠΆΠ΅ Π°Π³ΡΠ΅ΡΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄Π° ΠΊ Π±Π°Π·ΠΈΡΠ½ΠΎΠΈΜ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΡΠΎΡΡΠΎΡΠ½ΠΈΠ΅ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠ° ΡΡΠ°Π±ΠΈΠ»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π»ΠΎΡΡ.
Developing the Creative Potential of Future Engineers: Possibilities of Modern Education
Π ΡΡΠ°ΡΡΠ΅ Π΄Π°Π½ Π°Π½Π°Π»ΠΈΠ· ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΡ
ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½ΠΈΠΉ ΠΎ ΡΠ°Π·Π²ΠΈΡΠΈΠΈ ΠΊΡΠ΅Π°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»Π° ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°. ΠΠ²ΡΠΎΡΡ ΠΏΡΠΈΠ΄Π΅ΡΠΆΠΈΠ²Π°ΡΡΡΡ ΠΏΠΎΠ·ΠΈΡΠΈΠΈ ΠΎ Π½Π΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΠΎΡΡΠΈ ΠΏΡΠ΅ΠΎΠ΄ΠΎΠ»Π΅Π½ΠΈΡ ΡΠ·ΠΊΠΎ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄Π°, ΠΊΠ°ΠΊ Π² ΠΏΠΎΠ½ΠΈΠΌΠ°Π½ΠΈΠΈ ΠΊΡΠ΅Π°ΡΠΈΠ²Π½ΠΎΡΡΠΈ, ΡΠ°ΠΊ ΠΈ Π² ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΠΌ ΠΏΡΠΎΡΠ΅ΡΡΠ΅, ΠΎΡΠΈΠ΅Π½ΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΌ Π½Π° ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ ΠΊΡΠ΅Π°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»Π° ΡΡΡΠ΄Π΅Π½ΡΠΎΠ², ΡΡΠΎ ΡΡΠ°Π½ΠΎΠ²ΠΈΡΡΡ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΡΠΌ Π² ΡΠ°ΠΌΠΊΠ°Ρ
ΡΠΎΡΠΈΠΎΠΊΡΠ»ΡΡΡΡΠ½ΠΎΠΉ ΠΌΠΎΠ΄Π΅Π»ΠΈ ΠΊΡΠ΅Π°ΡΠΈΠ²Π½ΠΎΡΡΠΈ. ΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Π° ΡΡΡΡΠΊΡΡΡΠ° ΡΠΎΡΠΈΠΎΠΊΡΠ»ΡΡΡΡΠ½ΠΎΠΉ ΠΌΠΎΠ΄Π΅Π»ΠΈ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΊΡΠ΅Π°ΡΠΈΠ²Π½ΠΎΡΡΠΈ Π² ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠΈ. ΠΠΏΠΈΡΠ°ΡΡΡ Π½Π° ΡΠΎΠ±ΡΡΠ²Π΅Π½Π½ΡΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ, Π° ΡΠ°ΠΊΠΆΠ΅ Π½Π° ΠΎΡΠ΅ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠΉ ΠΈ Π·Π°ΡΡΠ±Π΅ΠΆΠ½ΡΠΉ ΠΎΠΏΡΡ, Π°Π²ΡΠΎΡΡ Π΄Π΅Π»Π°ΡΡ Π²ΡΠ²ΠΎΠ΄, ΡΡΠΎ ΡΠΎΠ»ΡΠΊΠΎ Π² ΡΠΎΠ²ΠΌΠ΅ΡΡΠ½ΠΎΠΉ ΠΊΡΠ΅Π°ΡΠΈΠ²Π½ΠΎΠΉ Π΄Π΅ΡΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ, Π·Π°Π²Π΅ΡΡΠ°ΡΡΠ΅ΠΉΡΡ ΡΠΎΠ·Π΄Π°Π½ΠΈΠ΅ΠΌ ΠΊΡΠ΅Π°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΠ΄ΡΠΊΡΠ°, ΠΈΠΌΠ΅ΡΡΠ΅Π³ΠΎ ΡΠΎΡΠΈΠ°Π»ΡΠ½ΡΡ Π·Π½Π°ΡΠΈΠΌΠΎΡΡΡ, Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎ Π½Π΅ Π°Π±ΡΡΡΠ°ΠΊΡΠ½ΠΎ - Π°ΠΊΠ°Π΄Π΅ΠΌΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΎΡΠ²ΠΎΠ΅Π½ΠΈΠ΅ ΡΠΎΡΠΌ ΠΊΡΠ΅Π°ΡΠΈΠ²Π½ΠΎΠΉ Π΄Π΅ΡΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ, Π½ΠΎ Π΅Π΅ Π»ΠΈΡΠ½ΠΎΡΡΠ½ΠΎΠ΅ ΠΏΡΠΈΡΠ²ΠΎΠ΅Π½ΠΈΠ΅. ΠΡΠΎΠ±Π΅Π½Π½ΠΎ ΡΡΠΎ Π·Π½Π°ΡΠΈΠΌΠΎ Π΄Π»Ρ Π±ΡΠ΄ΡΡΠΈΡ
ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠΎΠ². Π ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠΈ Π΄Π»Ρ ΡΡΠΎΠ³ΠΎ ΠΎΡΠΊΡΡΠ²Π°ΡΡΡΡ Π½ΠΎΠ²ΡΠ΅ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΠΊΠ°ΠΊ Π² ΡΠ²ΡΠ·ΠΈ Ρ Π²ΠΎΠ²Π»Π΅ΡΠ΅Π½ΠΈΠ΅ΠΌ ΡΡΡΠ΄Π΅Π½ΡΠΎΠ² Π² ΠΏΡΠΎΡΠ΅ΡΡ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΊΡΠ΅Π°ΡΠΈΠ²Π½ΡΡ
ΠΈΠ½Π΄ΡΡΡΡΠΈΠΉ, ΡΠ°ΠΊ ΠΈ Ρ ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ΠΌ ΠΊΠΎΠ½Π²Π΅ΡΠ³Π΅Π½ΡΠ½ΠΎΠΉ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΡΡΠ΅Π΄Ρ.The article analyzes modern ideas about the development of a person's creative potential. The authors adhere to the position on the need to overcome the narrowly technological approach, both in understanding creativity and in the educational process focused on the development of the creative potential of students, which becomes possible within the framework of the socio-cultural model of creativity. The structure of the socio-cultural model for the development of creativity in education is presented. Based on their own research, as well as on domestic and foreign experience, the authors conclude that only in joint creative activity, culminating in the creation of a creative product that has social significance, it is possible not abstractly - academic development of forms of creative activity, but its personal appropriation. This is especially important for future engineers. In education, new opportunities for this are opening up both in connection with the involvement of students in the development of creative industries, and with the development of a convergent educational environment
Prevalence of alcohol use among student youth in Yekaterinburg
The purpose of the study- Π°ssess the prevalence of alcohol use among students and factors influencing alcohol use.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ - ΠΎΡΠ΅Π½ΠΈΡΡ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΠΎΡΡΡ ΡΠΏΠΎΡΡΠ΅Π±Π»Π΅Π½ΠΈΡ Π°Π»ΠΊΠΎΠ³ΠΎΠ»Ρ ΡΡΠ΅Π΄ΠΈ ΡΡΡΠ΄Π΅Π½ΡΠΎΠ² ΠΈ ΡΠ°ΠΊΡΠΎΡΡ, Π²Π»ΠΈΡΡΡΠΈΠ΅ Π½Π° ΡΠΏΠΎΡΡΠ΅Π±Π»Π΅Π½ΠΈΠ΅ Π°Π»ΠΊΠΎΠ³ΠΎΠ»Ρ
ΠΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ Ρ Π²Π·ΡΠΎΡΠ»ΡΡ Π±ΠΎΠ»ΡΠ½ΡΡ ΠΌΡΠΊΠΎΠ²ΠΈΡΡΠΈΠ΄ΠΎΠ·ΠΎΠΌ β Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ "ΠΌΡΠ³ΠΊΠΈΡ " ΠΌΡΡΠ°ΡΠΈΠΉ
Clinical course of cystic fibrosis on adult patients carrying "mild" mutations.ΠΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ Ρ Π²Π·ΡΠΎΡΠ»ΡΡ
Π±ΠΎΠ»ΡΠ½ΡΡ
ΠΌΡΠΊΠΎΠ²ΠΈΡΡΠΈΠ΄ΠΎΠ·ΠΎΠΌ β Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ "ΠΌΡΠ³ΠΊΠΈΡ
" ΠΌΡΡΠ°ΡΠΈΠΉ
ΠΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ ΠΌΡΠΊΠΎΠ²ΠΈΡΡΠΈΠ΄ΠΎΠ·Π° Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ², ΠΆΠΈΡΠ΅Π»Π΅ΠΉ Π§ΡΠ²Π°ΡΡΠΊΠΎΠΉ Π Π΅ΡΠΏΡΠ±Π»ΠΈΠΊΠΈ
Summary. The paper presents an analysis of clinical, genetic, microbiological and functional findings of 51 patients with cystic fibrosis living in the Chuvash Republic. Data of neonatal screening for cystic fibrosis in this region are shown. The findings suggest a significant prevalence of the "mild" E92K mutation in the patients' genetic profile. Clinically, the "mild" genotype was related to the absence of pancreatic injury as one of the major CF signs and significantly fewer digestive complications. Several aspects of Π‘F treatment are highlighted in the article. Early, immediately after diagnosis, administration of dornase alpha (Pulmozyme) could prevent chronic inflammation in the lungs. Regular treatment with dornase alpha could delay development of irreversible pulmonary complications, improve quality of life of the children during an intensive growth and maintain lung function. Improved adherence to treatment in adult patients could improve the prognosis and bring hope to a long active life.Π Π΅Π·ΡΠΌΠ΅. Π ΡΡΠ°ΡΡΠ΅ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Ρ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ Π°Π½Π°Π»ΠΈΠ·Π° ΠΊΠ»ΠΈΠ½ΠΈΠΊΠΎ-Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
, ΠΌΠΈΠΊΡΠΎΠ±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Ρ 51 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠ° Ρ ΠΌΡΠΊΠΎΠ²ΠΈΡΡΠΈΠ΄ΠΎΠ·ΠΎΠΌ (ΠΠ), Π²ΡΠ΅ β ΠΆΠΈΡΠ΅Π»ΠΈ Π§ΡΠ²Π°ΡΡΠΊΠΎΠΉ Π Π΅ΡΠΏΡΠ±Π»ΠΈΠΊΠΈ. ΠΡΠΈΠ²Π΅Π΄Π΅Π½Ρ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ Π½Π΅ΠΎΠ½Π°ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΊΡΠΈΠ½ΠΈΠ½Π³Π° Π½Π° ΠΠ Π² Π΄Π°Π½Π½ΠΎΠΌ ΡΠ΅Π³ΠΈΠΎΠ½Π΅. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠΌ ΠΏΡΠ΅ΠΎΠ±Π»Π°Π΄Π°Π½ΠΈΠΈ "ΠΌΡΠ³ΠΊΠΎΠΉ" ΠΌΡΡΠ°ΡΠΈΠΈ E92K Π² Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΏΡΠΎΡΠΈΠ»Π΅ Π±ΠΎΠ»ΡΠ½ΡΡ
. "ΠΡΠ³ΠΊΠΈΠΉ" Π³Π΅Π½ΠΎΡΠΈΠΏ Π² ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΠ°ΡΡΠΈΠ½Π΅ Π²ΡΡΠ°ΠΆΠ΅Π½ ΠΎΡΡΡΡΡΡΠ²ΠΈΠ΅ΠΌ Π²Π°ΠΆΠ½Π΅ΠΉΡΠ΅ΠΉ Π² Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ΅ ΠΠ ΡΠΈΠΌΠΏΡΠΎΠΌΠ°ΡΠΈΠΊΠΈ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΡ ΠΏΠΎΠ΄ΠΆΠ΅Π»ΡΠ΄ΠΎΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ, Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ ΠΌΠ΅Π½ΡΡΠΈΠΌ ΡΠΈΡΠ»ΠΎΠΌ ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠΉ ΡΠΎ ΡΡΠΎΡΠΎΠ½Ρ ΠΎΡΠ³Π°Π½ΠΎΠ² ΠΏΠΈΡΠ΅Π²Π°ΡΠ΅Π½ΠΈΡ. ΠΡΠ²Π΅ΡΠ΅Π½Ρ Π½Π΅ΠΊΠΎΡΠΎΡΡΠ΅ Π°ΡΠΏΠ΅ΠΊΡΡ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ. Π Π°Π½Π½Π΅Π΅, ΡΡΠ°Π·Ρ ΠΏΠΎΡΠ»Π΅ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΡ Π΄ΠΈΠ°Π³Π½ΠΎΠ·Π°, Π½Π°Π·Π½Π°ΡΠ΅Π½ΠΈΠ΅ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ° ΠΡΠ»ΡΠΌΠΎΠ·ΠΈΠΌ ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΠ΅Ρ ΠΏΡΠΎΡΠΈΠ»Π°ΠΊΡΠΈΠΊΠ΅ ΡΠ°Π·Π²ΠΈΡΠΈΡ Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΡ Π² Π»Π΅Π³ΠΊΠΈΡ
. Π Π΅Π³ΡΠ»ΡΡΠ½ΠΎΠ΅ Π»Π΅ΡΠ΅Π½ΠΈΠ΅ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠΌ ΠΡΠ»ΡΠΌΠΎΠ·ΠΈΠΌ ΠΏΡΠ΅Π΄ΡΠΏΡΠ΅ΠΆΠ΄Π°Π΅Ρ ΡΠ°Π½Π½Π΅Π΅ ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ Π½Π΅ΠΎΠ±ΡΠ°ΡΠΈΠΌΡΡ
Π»Π΅Π³ΠΎΡΠ½ΡΡ
ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠΉ, ΡΠ»ΡΡΡΠ°Π΅Ρ ΠΊΠ°ΡΠ΅ΡΡΠ²ΠΎ ΠΆΠΈΠ·Π½ΠΈ Π΄Π΅ΡΠ΅ΠΉ Π² ΠΏΠ΅ΡΠΈΠΎΠ΄ ΠΈΠ½ΡΠ΅Π½ΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΡΠΎΡΡΠ°, Π΄Π°Π΅Ρ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ ΡΠΎΡ
ΡΠ°Π½ΠΈΡΡ Π»Π΅Π³ΠΎΡΠ½ΡΡ ΡΡΠ½ΠΊΡΠΈΡ, Π±Π»ΠΈΠ·ΠΊΡΡ ΠΊ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΠΎΠΉ. ΠΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ ΠΏΡΠΈΠ²Π΅ΡΠΆΠ΅Π½Π½ΠΎΡΡΠΈ Π»Π΅ΡΠ΅Π½ΠΈΡ ΡΡΠ΅Π΄ΠΈ Π²Π·ΡΠΎΡΠ»ΡΡ
ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² ΡΠ»ΡΡΡΠ°Π΅Ρ ΠΏΡΠΎΠ³Π½ΠΎΠ· ΠΆΠΈΠ·Π½ΠΈ, Π΄Π°Π΅Ρ Π½Π°Π΄Π΅ΠΆΠ΄Ρ Π½Π° Π΄ΠΎΠ»Π³ΡΡ Π°ΠΊΡΠΈΠ²Π½ΡΡ Π²Π·ΡΠΎΡΠ»ΡΡ ΠΆΠΈΠ·Π½Ρ
Asc1 Supports Cell-Wall Integrity Near Bud Sites by a Pkc1 Independent Mechanism
Background: The yeast ribosomal protein Asc1 is a WD-protein family member. Its mammalian ortholog, RACK1 was initially discovered as a receptor for activated protein C kinase (PKC) that functions to maintain the active conformation of PKC and to support its movement to target sites. In the budding yeast though, a connection between Asc1p and the PKC signaling pathway has never been reported. Methodology/Principal Findings: In the present study we found that asc1-deletion mutant (asc1D) presents some of the hallmarks of PKC signaling mutants. These include an increased sensitivity to staurosporine, a specific Pkc1p inhibitor, and susceptibility to cell-wall perturbing treatments such as hypotonic- and heat shock conditions and zymolase treatment. Microscopic analysis of asc1D cells revealed cell-wall invaginations near bud sites after exposure to hypotonic conditions, and the dynamic of cells β survival after this stress further supports the involvement of Asc1p in maintaining the cell-wall integrity during the mid-to late stages of bud formation. Genetic interactions between asc1 and pkc1 reveal synergistic sensitivities of a double-knock out mutant (asc1D/pkc1D) to cell-wall stress conditions, and high basal level of PKC signaling in asc1D. Furthermore, Asc1p has no effect on the cellular distribution or redistribution of Pkc1p at optimal or at cell-wall stress conditions. Conclusions/Significance: Taken together, our data support the idea that unlike its mammalian orthologs, Asc1p act
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