Disrupting Childhood Trauma with Holistic Health Practices in Low SES Elementary Schools

It is evident that adverse childhood experiences (ACE) are a large challenge to tackle in the U.S., with 61% of adults reporting that they’ve experienced one ACE and 16% reporting four or more ACEs by age 18 (Centers for Disease Control and Prevention, 2019). ACE score items include, but are not limited to experiencing verbal, physical, sexual abuse, physical and/or emotional neglect, having a mentally ill family member, witnessing violence in the home towards a parent, and/or having a family member in prison. Such experiences serve as a public health threat that, without early intervention, bring about long-term effects biologically, psychologically, and socially; with this, an adequate comprehension of the lasting implications of trauma can enhance and serve as foundational knowledge in establishing potential interventions (Watters & Martin, 2021; van der Kolk, 2014). Fortunately, the effects of ACEs can be disrupted, and research shows that schools could be a primary resource for children to get the support they need. According to the National Center for Education, on average, children in the United States spend 6.64 hours daily in school, with 180 days (about 6 months) out of the year being school days (Xianglei et al., 2019). More than half of a child’s life is spent within the school setting, making elementary school settings a crucial candidate for preventing and treating mental health issues and disorders from a young age. Unfortunately, schools often lack the capacity to provide adequate trauma informed care, especially schools located in areas of low socioeconomic status. Research shows that current education systems can be revised to support traumatized youth and improve emotional, physical, and academic well-being. More studies point to the association between holistic healing practices, a form of trauma-informed care that can be accessible to all communities, and effective biopsychosocial regulation (Bazzano, et al., 2018; Cozzolino et al., 2022; Eads, 2022; Mayer, 2019; Sarkissian Leadership, 2018). Hence, in order to disrupt the effects of trauma on a child’s body, mind, overall wellbeing, and enhance success in school, elementary schools should implement strategic trauma informed protocols in order to holistically support their students. The purpose of this paper is to offer a review of the literature that studied accessible holistic health practices for low SES elementary school settings to support healthy development of children who have experienced and are experiencing adversity

TransRate: reference-free quality assessment of de novo transcriptome assemblies.

TransRate is a tool for reference-free quality assessment of de novo transcriptome assemblies. Using only the sequenced reads and the assembly as input, we show that multiple common artifacts of de novo transcriptome assembly can be readily detected. These include chimeras, structural errors, incomplete assembly, and base errors. TransRate evaluates these errors to produce a diagnostic quality score for each contig, and these contig scores are integrated to evaluate whole assemblies. Thus, TransRate can be used for de novo assembly filtering and optimization as well as comparison of assemblies generated using different methods from the same input reads. Applying the method to a data set of 155 published de novo transcriptome assemblies, we deconstruct the contribution that assembly method, read length, read quantity, and read quality make to the accuracy of de novo transcriptome assemblies and reveal that variance in the quality of the input data explains 43% of the variance in the quality of published de novo transcriptome assemblies. Because TransRate is reference-free, it is suitable for assessment of assemblies of all types of RNA, including assemblies of long noncoding RNA, rRNA, mRNA, and mixed RNA samples

Військовополонені Першої світової війни в Кременчуцькому гарнізоні

У статті досліджено правове становище та умови перебування військовополонених Першої світової війни на території Кременчуцького гарнізону в другій половині 1916 року. На основі архівних даних визначено кількісний склад полонених, його розподіл за національною, соціальною та конфесійною ознаками. Досліджено динаміку чисельності, побутові умови та використання праці військовополонених.В статье исследовано правовое положение и условия пребывания военнопленных Первой мировой войны на территории Кременчугского гарнизона во второй половине 1916 года. На основе архивных данных определен количественный состав пленных, его распределение по национальной, социальной и конфессиональной принадлежности. Исследована динамика численности, бытовые условия и использования труда военнопленных.In article the legal status and conditions of stay of prisoners of war of the First World War in territory of the Kremenchug garrison in second half of 1916 is investigated. On the basis of contemporary records the quantitative structure captured, its distribution on a national, social and confessional accessory is defi ned. Dynamics of number, conditions of life and uses of work of prisoners of war is investigated

Проблемы специализированной помощи обожженным в России и пути их решения

На основании изучения динамики ожогового травматизма в России, тяжести травм и летальности среди больных с ожогами отмечено увеличение тяжести термической травмы, частоты раннего ожогового сепсиса и летальности среди взрослого населения, определены организационные и научные пути решения проблемы, повышения эффективности работы ожоговых центров.Basing on the study of the dynamics of burns in Russia, severity of the injury and death rate in the patients with burns the authors emphasize the increase in severity of thermal injury, incidence of early burn sepsis and death rate in adults. Organizational and scientific ways to solve the problem as well as to increase the efficacy of work of burn centers are defined

Shared characteristics underpinning C 4 leaf maturation derived from analysis of multiple C 3 and C 4 species of Flaveria

Most terrestrial plants use C3 photosynthesis to fix carbon. In multiple plant lineages a modified system known as C4 photosynthesis has evolved. To better understand the molecular patterns associated with induction of C4 photosynthesis, the genus Flaveria that contains C3 and C4 species was used. A base to tip maturation gradient of leaf anatomy was defined, and RNA sequencing was undertaken along this gradient for two C3 and two C4Flaveria species. Key C4 traits including vein density, mesophyll and bundle sheath cross-sectional area, chloroplast ultrastructure, and abundance of transcripts encoding proteins of C4 photosynthesis were quantified. Candidate genes underlying each of these C4 characteristics were identified. Principal components analysis indicated that leaf maturation and the photosynthetic pathway were responsible for the greatest amount of variation in transcript abundance. Photosynthesis genes were over-represented for a prolonged period in the C4 species. Through comparison with publicly available data sets, we identify a small number of transcriptional regulators that have been up-regulated in diverse C4 species. The analysis identifies similar patterns of expression in independent C4 lineages and so indicates that the complex C4 pathway is associated with parallel as well as convergent evolution

Deep evolutionary comparison of gene expression identifies parallel recruitment of trans-factors in two independent origins of C4 photosynthesis

With at least 60 independent origins spanning monocotyledons and dicotyledons, the C(4) photosynthetic pathway represents one of the most remarkable examples of convergent evolution. The recurrent evolution of this highly complex trait involving alterations to leaf anatomy, cell biology and biochemistry allows an increase in productivity by ∼50% in tropical and subtropical areas. The extent to which separate lineages of C(4) plants use the same genetic networks to maintain C(4) photosynthesis is unknown. We developed a new informatics framework to enable deep evolutionary comparison of gene expression in species lacking reference genomes. We exploited this to compare gene expression in species representing two independent C(4) lineages (Cleome gynandra and Zea mays) whose last common ancestor diverged ∼140 million years ago. We define a cohort of 3,335 genes that represent conserved components of leaf and photosynthetic development in these species. Furthermore, we show that genes encoding proteins of the C(4) cycle are recruited into networks defined by photosynthesis-related genes. Despite the wide evolutionary separation and independent origins of the C(4) phenotype, we report that these species use homologous transcription factors to both induce C(4) photosynthesis and to maintain the cell specific gene expression required for the pathway to operate. We define a core molecular signature associated with leaf and photosynthetic maturation that is likely shared by angiosperm species derived from the last common ancestor of the monocotyledons and dicotyledons. We show that deep evolutionary comparisons of gene expression can reveal novel insight into the molecular convergence of highly complex phenotypes and that parallel evolution of trans-factors underpins the repeated appearance of C(4) photosynthesis. Thus, exploitation of extant natural variation associated with complex traits can be used to identify regulators. Moreover, the transcription factors that are shared by independent C(4) lineages are key targets for engineering the C(4) pathway into C(3) crops such as rice

Evolutionary convergence of cell-specific gene expression in independent lineages of C-4 grasses

Leaves of almost all C(4) lineages separate the reactions of photosynthesis into the mesophyll (M) and bundle sheath (BS). The extent to which messenger RNA profiles of M and BS cells from independent C(4) lineages resemble each other is not known. To address this, we conducted deep sequencing of RNA isolated from the M and BS of Setaria viridis and compared these data with publicly available information from maize (Zea mays). This revealed a high correlation (r = 0.89) between the relative abundance of transcripts encoding proteins of the core C(4) pathway in M and BS cells in these species, indicating significant convergence in transcript accumulation in these evolutionarily independent C(4) lineages. We also found that the vast majority of genes encoding proteins of the C(4) cycle in S. viridis are syntenic to homologs used by maize. In both lineages, 122 and 212 homologous transcription factors were preferentially expressed in the M and BS, respectively. Sixteen shared regulators of chloroplast biogenesis were identified, 14 of which were syntenic homologs in maize and S. viridis. In sorghum (Sorghum bicolor), a third C(4) grass, we found that 82% of these trans-factors were also differentially expressed in either M or BS cells. Taken together, these data provide, to our knowledge, the first quantification of convergence in transcript abundance in the M and BS cells from independent lineages of C(4) grasses. Furthermore, the repeated recruitment of syntenic homologs from large gene families strongly implies that parallel evolution of both structural genes and trans-factors underpins the polyphyletic evolution of this highly complex trait in the monocotyledons

The Stern-Gerlach Experiment Revisited

The Stern-Gerlach-Experiment (SGE) of 1922 is a seminal benchmark experiment of quantum physics providing evidence for several fundamental properties of quantum systems. Based on today's knowledge we illustrate the different benchmark results of the SGE for the development of modern quantum physics and chemistry. The SGE provided the first direct experimental evidence for angular momentum quantization in the quantum world and thus also for the existence of directional quantization of all angular momenta in the process of measurement. It measured for the first time a ground state property of an atom, it produced for the first time a `spin-polarized' atomic beam, it almost revealed the electron spin. The SGE was the first fully successful molecular beam experiment with high momentum-resolution by beam measurements in vacuum. This technique provided a new kinematic microscope with which inner atomic or nuclear properties could be investigated. The original SGE is described together with early attempts by Einstein, Ehrenfest, Heisenberg, and others to understand directional quantization in the SGE. Heisenberg's and Einstein's proposals of an improved multi-stage SGE are presented. The first realization of these proposals by Stern, Phipps, Frisch and Segr\`e is described. The set-up suggested by Einstein can be considered an anticipation of a Rabi-apparatus. Recent theoretical work is mentioned in which the directional quantization process and possible interference effects of the two different spin states are investigated. In full agreement with the results of the new quantum theory directional quantization appears as a general and universal feature of quantum measurements. One experimental example for such directional quantization in scattering processes is shown. Last not least, the early history of the `almost' discovery of the electron spin in the SGE is revisited.Comment: 50pp, 17 fig

Mental capacity to consent to treatment and the association with outcome:A longitudinal study in patients with anorexia nervosa

BACKGROUND: Relevance of diminished mental capacity in anorexia nervosa (AN) to course of disorder is unknown. AIMS: To examine prognostic relevance of diminished mental capacity in AN. METHOD: A longitudinal study was conducted in 70 adult female patients with severe AN. At baseline, mental capacity was assessed by psychiatrists, and clinical and neuropsychological data (decision-making) were collected. After 1 and 2 years, clinical and neuropsychological assessments were repeated, and remission and admission rates were calculated. RESULTS: People with AN with diminished mental capacity had a less favourable outcome with regard to remission and were admitted more frequently. Their appreciation of illness remained hampered. Decision-making did not improve, in contrast to people with full mental capacity. CONCLUSIONS: People with AN with diminished mental capacity seem to do less well in treatment and display decision-making deficiencies that do not ameliorate with weight improvement