36 research outputs found
Implementasi manajemen dakwah pada Koperasi Serba Usaha Syariah (KSUS) Surya Perkasa Lamongan
Penelitian ini ditulis oleh M. Ardian Choiru Tasbihi Hisbullah (1601036032) dengan judul: IMPLEMENTASI MANAJEMEN DAKWAH PADA KOPERASI SERBA USAHA SYARIAH (KSUS) SURYA PERKASA LAMONGAN. Penelitian ini merupakan salah satu upaya penulis untuk mencoba mengetahui bagaimana implementasi manajemen dakwah di Koperasi Serba Usaha Syariah (KSUS) Surya Perkasa Lamongan dan apa saja faktor pendukung dan penghambat di Koperasi Serba Usaha Syariah (KSUS) Surya Perkasa Lamongan.
Jenis penelitian ini adalah penelitian kualitatif dengan metode penelitian kualitatif deskriptif yaitu data yang terkumpul berbentuk kata-kata, gambar bukan angka-angka. Jika terdapat data yang berbentuk angka-angka, sifatnya hanya sebagai penunjang. Data primer adalah data yang diperoleh dari Pengurus Koperasi Serba Usaha Syariah (KSUS) Surya Perkasa Lamongan. Data sekunder adalah data yang diperoleh dari dokumentasi, arsip, struktur organisasi, internet dan buku-buku yang ada relevansinya dengan Implementasi manajemen dakwah. Metode pengumpulan data yang digunakan oleh peneliti adalah observasi, wawancara dan dokumentasi.
Hasil penelitian menunjukkan bahwa Implementasi Manajemen Dakwah dalam Pengelolaan Koperasi Serba Usaha Syariah (KSUS) Surya Perkasa Lamongan adalah: (1) Dalam Implementasi Manajemen Dakwah di Koperasi Serba Usaha Syariah (KSUS) Surya Perkasa Lamongan menggunakan fungsi manajemen dakwah yaitu: perencanaan dakwah (takhthith), pengorganisasian dakwah (Thanzim), penggerakan dakwah (tawjih), dan pengendalian dan evaluasi dakwah (Riqabah). (2) Sedangkan factor pendukung dan penghambat adalah, factor pendukung: lokasi yang mudah dijangkau, pelayanan prima terhadap nasabah, masyarakat mulai tertarik pada koperasi yang sistem operasionalnya berbasis syariah. Adapun factor penghambatnya adalah: Pengetahuan masyarakat masih minim tentang koperasi simpan pinjam Koperasi Serba Usaha Syariah (KSUS) Surya Perkasa Lamongan, tingkat pesaing tinggi, berprasangka baik terhadap nasabah, kurangnya kesadaran dari anggota
The Role of Liposomal CpG ODN on the Course of L. major Infection in BALB/C Mice
"nBackground: Historically, leishmanization is the most effective protective measure against Cutaneous Leishmaniasis (CL), CL lesion induced by leishmanization sometimes takes a long time to heal. Manipulation of leishmanization inoculums needed to induce a mild and acceptable CL lesion. The aim of this study was to explore if liposomal form of CpG ODN (Cytosin phosphate Guanin Oligodeoxynucleotides) mixed with Leishmania major would induce a milder lesion size in Balb/c mice."nMethods: This study was performed in Biotechnology Research Center, Mashhad, and Center for Research and Training in Skin Diseases and Leprosy, Tehran, Iran during 2008-2009. mice were subcutaneously (SC) inoculated with L. major mixed with liposomal form of CpG ODN, or L. major plus free CpG ODN, or L. major mixed with empty liposomes or L. major in PBS. The lesion onset and the size of lesion were recorded; the death rate was also monitored. "nResult: Footpad thickness was significantly (P<0.01) smaller, death rate was also significantly (P<0.05) lower in the mice received L. major mixed with liposomal CpG ODN or free CpG ODN than control groups received L. major in PBS or L. major plus liposomes, also mice which received L. major mixed with CpG ODN in soluble form showed a significantly (P < 0.001) smaller lesion size than control groups."nConclusion: CpG ODN seems to be an appropriate immunopotentiator mixed with Leishmania stabilate in leishmanization
Screening of Heterogeneous Photocatalysts for Water Splitting
In this contribution, a simple method for the screening of photocatalytic activity of catalyst materials is presented. The method is based on two steps the immobilization of the photocatalyst and the subsequent testing of their photocatalytic activity, using the gas evolution at the solid liquid interface. Up to four catalysts can be tested under the same conditions. The observed gas evolution for selected photocatalysts is consistent with trends reported in the literature from conventional photocatalytic reactor
Selective isolation and characterization of primary cells from normal breast and tumors reveal plasticity of adipose derived stem cells
Low-temperature inorganic synthesis and photocatalytic activity of anatase TiO2 powders
Nowadays, it is a great challenge to synthesize different crystalline TiO2 using low temperature methods without calcination step. In our study, the nanocrystalline titanium dioxide sols were prepared from TiCl4 under acidic conditions. The powder was obtained with consequent drying of the sols at temperatures below 100 oC and these samples were then characterized using different techniques. The results show that the properties of the asprepared powders depend mainly on the type of the acid and less on the amount of the acid. The usage of HClO4 instead of HNO3 as the peptizing agent results in colloids with higher photocatalytic activit
Comparative mitochondrial proteomics of Leishmania tropica clinical isolates resistant and sensitive to meglumine antimoniate
Antimony is an important drug for the treatment of Leishmania parasite infections. In several countries, the emergence of drug-resistant Leishmania species has reduced the effectiveness of this drug. The mechanism of clinical drug resistance is unclear. The aim of this work was to identify mitochondrial proteome alterations associated with resistance against antimonial. A combination of cell fractionation, liquid chromatography-tandem mass spectrometry (LC-MS/MS), and Label-Free Quantification was used to characterize the mitochondrial protein composition of Leishmania tropica field isolates resistant and sensitive to meglumine antimoniate. LC-MS/MS analysis resulted in the identification of about 1200 proteins of the Leishmania tropica mitochondrial proteome. Various criteria were used to allocate about 40 proteins to mitochondrial proteome. Comparative quantitative proteomic analysis of the sensitive and the resistant strains showed proteins with differential abundance in resistance species are involved in TCA and aerobic respiration enzymes, stress proteins, lipid metabolism enzymes, and translation. These results showed that the mechanism of antimony resistance in Leishmania spp. field isolate may be associated with alteration in enzymes involved in mitochondrial pathways. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature
A short review on photocatalytic degradation of formaldehyde
Nowadays, it is a great challenge to eliminate toxic and harmful organic pollutants from air and water. This paper reviews the role of TiO2 as a photocatalyst, light source and photoreactor in the particular case of removal of formaldehyde using the photocatalytic reaction by titanium dioxide (TiO2 in aqueous and gaseous systems. The reaction mechanisms of the photocatalytic oxidation of gaseous formaldehyde are given. We also present a detailed review of published articles on photocatalytic degradation of formaldehyde by modified titanium dioxide doped with foreign species such as metal and non-metal components. We point out the most prospective developments of the photocatalyst compositions for the future potential commercial applications
Mitochondrial proteome profiling of Leishmania tropica
The mitochondrion of kinetoplastida has unique characteristics both in structure and function. To better understand the mitochondrial proteome of the Leishmania tropica promastigote stage, liquid chromatography coupled with mass spectrometry (LC/MS/MS) approach was used. In the wake of mitochondria isolation and purity validation, 1212 proteins were identified, among which approximately 44 of proteins belonged to the mitochondrial proteome. Several functions were enriched in mitochondrial proteome including tricarboxylic acid cycle and respiratory chain, protein folding, signalling, transport, lipid metabolism, amino acid, and nucleotide metabolism. Furthermore, the result of the present research was compared with the previous related studies. Gaining more information about vital metabolism of the cell and molecules can be used for therapeutic purposes. © 2019 Elsevier Lt
Selective isolation and characterization of primary cells from normal breast and tumors reveal plasticity of adipose derived stem cells
Background
There is a need to establish more cell lines from breast tumors in contrast to immortalized cell lines from metastatic effusions in order to represent the primary tumor and not principally metastatic biology of breast cancer. This investigation describes the simultaneous isolation, characterization, growth and function of primary mammary epithelial cells (MEC), mesenchymal cells (MES) and adipose derived stem cells (ADSC) from four normal breasts, one inflammatory and one triple-negative ductal breast tumors.
Methods
A total of 17 cell lines were established and gene expression was analyzed for MEC and MES (n = 42) and ADSC (n = 48) and MUC1, pan-KRT, CD90 and GATA-3 by immunofluorescence. DNA fingerprinting to track cell line identity was performed between original primary tissues and isolates. Functional studies included ADSC differentiation, tumor MES and MEC invasion co-cultured with ADSC-conditioned media (CM) and MES adhesion and growth on 3D-printed scaffolds.
Results
Comparative analysis showed higher gene expression of EPCAM, CD49f, CDH1 and KRTs for normal MEC lines; MES lines e.g. Vimentin, CD10, ACTA2 and MMP9; and ADSC lines e.g. CD105, CD90, CDH2 and CDH11. Compared to the mean of all four normal breast cell lines, both breast tumor cell lines demonstrated significantly lower ADSC marker gene expression, but higher expression of mesenchymal and invasion gene markers like SNAI1 and MMP2. When compared with four normal ADSC differentiated lineages, both tumor ADSC showed impaired osteogenic and chondrogenic but enhanced adipogenic differentiation and endothelial-like structures, possibly due to high PDGFRB and CD34. Addressing a functional role for overproduction of adipocytes, we initiated 3D-invasion studies including different cell types from the same patient. CM from ADSC differentiating into adipocytes induced tumor MEC 3D-invasion via EMT and amoeboid phenotypes. Normal MES breast cells adhered and proliferated on 3D-printed scaffolds containing 20 fibers, but not on 2.5D-printed scaffolds with single fiber layers, important for tissue engineering.
Conclusion
Expression analyses confirmed successful simultaneous cell isolations of three different phenotypes from normal and tumor primary breast tissues. Our cell culture studies support that breast-tumor environment differentially regulates tumor ADSC plasticity as well as cell invasion and demonstrates applications for regenerative medicine