Chromatographic Separation of Te(IV) & Te(VI)

443-44

Development of a RAD-Seq Based DNA Polymorphism Identification Software, AgroMarker Finder, and Its Application in Rice Marker-Assisted Breeding

Abstract Rapid and accurate genome-wide marker detection is essential to the marker-assisted breeding and functional genomics studies. In this work, we developed an integrated software, AgroMarker Finder (AMF: http://erp.novelbio.com/AMF), for providing graphical user interface (GUI) to facilitate the recently developed restriction-site associated DNA (RAD) sequencing data analysis in rice. By application of AMF, a total of 90,743 high-quality markers (82,878 SNPs and 7,865 InDels) were detected between rice varieties JP69 and Jiaoyuan5A. The density of the identified markers is 0.2 per Kb for SNP markers, and 0.02 per Kb for InDel markers. Sequencing validation revealed that the accuracy of genome-wide marker detection by AMF is 93%. In addition, a validated subset of 82 SNPs and 31 InDels were found to be closely linked to 117 important agronomic trait genes, providing a basis for subsequent marker-assisted selection (MAS) and variety identification. Furthermore, we selected 12 markers from 31 validated InDel markers to identify seed authenticity of variety Jiaoyuanyou69, and we also identified 10 markers closely linked to the fragrant gene BADH2 to minimize linkage drag for Wuxiang075 (BADH2 donor)/Jiachang1 recombinants selection. Therefore, this software provides an efficient approach for marker identification from RAD-seq data, and it would be a valuable tool for plant MAS and variety protection

Maize (Zea mays L.) Genome Diversity as Revealed by RNA-Sequencing

Maize is rich in genetic and phenotypic diversity. Understanding the sequence, structural, and expression variation that contributes to phenotypic diversity would facilitate more efficient varietal improvement. RNA based sequencing (RNA-seq) is a powerful approach for transcriptional analysis, assessing sequence variation, and identifying novel transcript sequences, particularly in large, complex, repetitive genomes such as maize. In this study, we sequenced RNA from whole seedlings of 21 maize inbred lines representing diverse North American and exotic germplasm. Single nucleotide polymorphism (SNP) detection identified 351,710 polymorphic loci distributed throughout the genome covering 22,830 annotated genes. Tight clustering of two distinct heterotic groups and exotic lines was evident using these SNPs as genetic markers. Transcript abundance analysis revealed minimal variation in the total number of genes expressed across these 21 lines (57.1% to 66.0%). However, the transcribed gene set among the 21 lines varied, with 48.7% expressed in all of the lines, 27.9% expressed in one to 20 lines, and 23.4% expressed in none of the lines. De novo assembly of RNA-seq reads that did not map to the reference B73 genome sequence revealed 1,321 high confidence novel transcripts, of which, 564 loci were present in all 21 lines, including B73, and 757 loci were restricted to a subset of the lines. RT-PCR validation demonstrated 87.5% concordance with the computational prediction of these expressed novel transcripts. Intriguingly, 145 of the novel de novo assembled loci were present in lines from only one of the two heterotic groups consistent with the hypothesis that, in addition to sequence polymorphisms and transcript abundance, transcript presence/absence variation is present and, thereby, may be a mechanism contributing to the genetic basis of heterosis

Oxidation and estimation of bilirubin by using carbon microelectrode

1239-1244Bilirubin is one of the major bile pigment, clinically and biologically important among the bile pigments present in the mammal. When the concentration or bilirubin exeeds the allowable limit, it leads to a number or disorders and ailments in the human beings including jaundice. Bi1irubin is present in the reduced form which is highly toxic whereas its oxidized form biliverdin is nontoxic. Hence the oxidation and estimation of bilirubin is essential in the clinical arena. A differential pulse voltammetric method has been recommended for<span style="font-size:13.0pt;mso-bidi-font-size: 7.0pt"> the estimation of bilirubin with a bare carbon microelectrode in the aqueous medium and indirect oxidation of bilirubin to biliverdin. The oxidation has been carried out using <i style="mso-bidi-font-style: normal">in situ electrogenerated hydrogen peroxide produced from the continuous reduction of oxygen at its reduction potential. Estimation of bilirubin has been done using its reduction peak observed at around -1.1V. The kinetic studies of indirect oxidation of bilirubin has been followed spectrophotometrically in the visible region. </span

Thin Layer Chromatographic Behaviour of Dithiocarbamates of Arsenic(III), Antimony (III) & Bismuth(III)

311-31

Paper Chromatographic Separation of Iron Valences via Their Anthranilates & Their Photometric Estimation as Pyridine Adducts

605-60

Thin Layer Chromatographic Behaviour of Dithiocarbamates of Zinc(II), Cadmi um(II) & Mercury(II)

402-40

Thin Layer Chromatographic Behaviour of Piperidinel-carbodithioates of Nickel(II), Copper(II), Zinc(II), Cobalt(III), Chromium(III), Manganese(III) & Iron(III)

399-40

Chromium(III) Dithiocarbamates-A Thin Layer Chromatographic Study & Characterisation of Some New Binuclear Mixed Ligand Dithiocarbarnates

1058-106