Assessing Personality Traits Through Response Latencies Using Item Response Theory

Recent studies have revealed a relation between the given response and the response latency for personality questionnaire items in the form of an inverted-U effect, which has been interpreted in light of schema-driven behavior. In general, more probable responses are given faster. In the present study, the relationship between the probability of the given response and the response latency was investigated. First, a probabilistic model was introduced describing the relationship between response latencies and a latent trait. Second, the model was applied in an empirical study: Employing items from a personality questionnaire and using data from 170 men, the probability of responses were estimated based on the Rasch model. Assuming log-normally distributed response latencies, a linear regression model was fit to the logarithmized response latencies, including the response probability as a predictor. Findings suggested that the quantities are negatively related. This relation can be used to incorporate the response latency into the estimation of trait levels. For the scales used in the study, the results showed that test information could be increased by 13% to 17% when considering response latencies

Stability of nuclear and mitochondrial reference genes in selected tissues of the Ambrosia beetle Xylosandrus germanus

SIMPLE SUMMARY: The ambrosia beetle Xylosandrus germanus (Blandford) is a destructive wood-boring insect of horticultural tree crops. A fungal mutualist is cultivated within host trees that provides the sole source of nutrition for the larvae and adults of this beetle. Female X. germanus adults use a pouch-like structure (i.e., mycangium) to maintain and transport spores of their fungal mutualist. To facilitate future studies examining gene expression of X. germanus’ mycangium, the identification of stable genes unaffected by experimental treatments is needed to provide a standard reference during gene expression studies. Selected tissue types were dissected from laboratory-reared and field-collected specimens of the ambrosia beetle X. germanus to evaluate the stability of five reference genes, namely, 28S ribosomal RNA (28S rRNA), arginine kinase (AK), carbamoyl-phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase (CAD), mitochondrial cytochrome oxidase 1 (CO1), and elongation factor-1α (EF1α). The reference genes CO1 and AK were identified as primary and secondary reference genes. By contrast, EF1α was considered unsuitable for use as a reference gene during gene expression studies with X. germanus. These results will aid in normalizing the expression of target genes during studies with X. germanus. ABSTRACT: The fungus-farming ambrosia beetle Xylosandrus germanus (Blandford) uses a pouch-like structure (i.e., mycangium) to transport spores of its nutritional fungal mutualist. Our current study sought to identify reference genes necessary for future transcriptome analyses aimed at characterizing gene expression within the mycangium. Complementary DNA was synthesized using selected tissue types from laboratory-reared and field-collected X. germanus consisting of the whole body, head + thorax, deflated or inflated mycangium + scutellum, inflated mycangium, and thorax + abdomen. Quantitative reverse-transcription PCR reactions were performed using primers for 28S ribosomal RNA (28S rRNA), arginine kinase (AK), carbamoyl-phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase (CAD), mitochondrial cytochrome oxidase 1 (CO1), and elongation factor-1α (EF1α). Reference gene stability was analyzed using GeNorm, NormFinder, BestKeeper, ΔCt, and a comprehensive final ranking by RefFinder. The gene CO1 was identified as the primary reference gene since it was generally ranked in first or second position among the tissue types containing the mycangium. Reference gene AK was identified as a secondary reference gene. In contrast, EF1α was generally ranked in the last or penultimate place. Identification of two stable reference genes will aid in normalizing the expression of target genes for subsequent gene expression studies of X. germanus’ mycangium

In situ study of soil processes in an oxisol-spodosol sequence of Amazonia (Brazil)

L'étude in situ de l'évolution d'échantillons de vermiculite et de résines à cations échangeables et kelates insérés dans le sol met en évidence les processsus pédogénétiques caractérisant une séquence de sols de la région de Manaus (Brésil

Monitoring Handbook 6: Analyzing and interpreting monitoring data

The basic purpose of data analysis is to identify patterns of change in your indicator over time, and to evaluate these changes. Without doing some kind of analysis, it will be difficult for you to know the effect your project is actually having. The data analysis techniques presented in this handbook are not difficult. Most of them can be easily done using little more than a calculator and scratch paper. If necessary, there are resources listed in the handbook for additional assistance analyzing your data

Greenhouse Evaluation of Air-Assisted Delivery Parameters for Mature Poinsettias

Understanding the performance characteristics of application equipment is important for helping make the most efficacious applications. While handguns making high volume applications are common in greenhouse production, it is difficult to achieve uniform distribution of product in a timely manner. Broadcast applications made using air-assistance can help aid canopy penetration and the volume of carrier required to make applications. The objectives of this research were to determine how air-assist sprayer application parameters influence spray deposits on the undersides of leaves in a mature poinsettia canopy. Bench-top trials were conducted using a motorized boom inside a greenhouse to treat a mature and dense poinsettia canopy. Sprayer treatments applied a tank mix of water and fluorescent tracer. Nylon screen targets were secured to the underside surfaces of leaves in the upper and lower elevation of target plants. A five-port, air-assist nozzle with flat fan nozzle tips was used to make the applications. Three air outlet speeds, two travel speeds, and three nozzle flow rates were evaluated. Each treatment was replicated three times. Spray deposits were highly variable. Upper elevation spray deposits were significantly greater than lower elevation deposits. Individually, higher air outlet speed (36.0 m s(-1)), slower travel speed (3.2 km h(-1)), and higher nozzle flow rate (1.17 L min(-1)) tended to produce higher sprayer deposits on the underside surfaces of leaves. The combination of travel speed and nozzle flow rate that produced the highest application rate (900 L ha(-1)) also produced the highest deposits. There was a 500% increase in underside leaf surface deposits in the lower canopy area for a corresponding 500% increase in application rate. However, the main effects produced no significant differences in spray deposits in the lower canopy area. Further improvements in directing sprays or providing canopy turbulence are necessary to improve deposition and management of insect pests feeding on the underside of poinsettia leaves

Ethanol Injection of Ornamental Trees Facilitates Testing Insecticide Efficacy Against Ambrosia Beetles (Coleoptera: Curculionidae: Scolytinae)

Exotic ambrosia beetles are damaging pests in ornamental tree nurseries in North America. The species Xylosandrus crassiusculus (Motshulsky) and Xylosandrus germanus (Blandford) are especially problematic. Management of these pests relies on preventive treatments of insecticides. However, field tests of recommended materials on nursery trees have been limited because of unreliable attacks by ambrosia beetles on experimental trees. Ethanol-injection of trees was used to induce colonization by ambrosia beetles to evaluate insecticides and botanical formulations for preventing attacks by ambrosia beetles. Experiments were conducted in Ohio, Tennessee, and Virginia. Experimental trees injected with ethanol had more attacks by ambrosia beetles than un-injected control trees in all but one experiment. Xylosandrus crassiusculus and X. germanus colonized trees injected with ethanol. In most experiments, attack rates declined 8 d after ethanol-injection. Ethanol-injection induced sufficient pressure from ambrosia beetles to evaluate the efficacy of insecticides for preventing attacks. Trunk sprays of permethrin suppressed cumulative total attacks by ambrosia beetles in most tests. Trunk sprays of the botanical formulations Armorex and Veggie Pharm suppressed cumulative total attacks in Ohio. Armorex, Armorex + Permethrin, and Veggie Pharm + Permethrin suppressed attacks in Tennessee. The bifenthrin product Onyx suppressed establishment of X. germanus in one Ohio experiment, and cumulative total ambrosia beetle attacks in Virginia. Substrate drenches and trunk sprays of neonicotinoids, or trunk sprays of anthranilic diamides or tolfenpyrad were not effective. Ethanol-injection is effective for inducing attacks and ensuring pressure by ambrosia beetles for testing insecticide efficacy on ornamental trees

Preharvest Quarantine Treatments of Chlorantraniliprole, Clothianidin, and Imidacloprid-Based Insecticides for Control of Japanese Beetle (Coleoptera: Scarabaeidae) and Other Scarab Larvae in the Root Zone of Field-Grown Nursery Trees

Japanese beetle, Popillia japonica Newman (Coleoptera: Scarabaeidae), is an important quarantine pest of nurseries. Nursery plant movement from P. japonica-infested regions is regulated by the U.S. Domestic Japanese Beetle Harmonization Plan (DJHP), which classifies states by risk categories. Treatments for category 2 states include preharvest soil surface treatment of nursery plants grown in field soil using Discus SC, Marathon (1G or 60 WP), or Flagship (0.22G or 25 WG). In this study, Discus, Marathon 60 WP, or Flagship 0.22G DJHP standards were compared with labeled rates of non–DJHP-approved insecticides, including neonicotinoids clothianidin (Arena 50WDG), generic imidacloprid (Quali-Pro Imidacloprid 2 F T&O Insecticide, Mallet 2 F T&O Insecticide, and Lada 2 F Insecticide), and imidacloprid + bifenthrin (Allectus SC), as well as the anthranilic diamide, chlorantraniliprole (Acelepryn Insecticide). Arena provided 100% P. japonica control in May, June, and July over four test years, but had one larva recovered during August in two of those 4 yr. Acelepryn did not provide DJHP-acceptable P. japonica control. During July, Allectus provided 100% P. japonica control in three of four test years, but had four larvae in one test year. Other treatments tested only during July, which provided 100% P. japonica control, included Discus (five tests); Marathon, Quali-Pro, and Mallet (two tests); and Lada and Flagship (one test). Generic imidacloprid 2 F formulations were equivalent in P. japonica control to DJHP-approved insecticides. Insecticides generally performed poorly on other scarabs or curculionid larvae. The study supports Arena, Allectus, and generic imidacloprid 2 F products as suitable candidates for the DJHP