Biochemical Investigation on the activities of Acid and Alkaline Phosphatases in two varieties of Carica papaya L. (Pawpaw) during ripening

The activities of acid phosphatase and alkaline phosphatase were investigated in two varieties of ripening Carica papaya fruit; Oblong-shaped variety which is also known as ‘Agric pawpaw’ and Pear-shaped variety which is also known as ‘Local pawpaw’. Acid phosphatase activity decreased significantly (p < 0.01) throughout the ripening stages from 0.0186±0.0006 to 0.0037 ± 0.0002μmol/min/g fresh weight in the Oblong-shaped variety: ‘Agric pawpaw’ and from 0.0134 ± 0.0008 to 0.0068 ± 0.0004μmol/min/g fresh weight in the Pear-shaped variety: ‘Local pawpaw’. Conversely, alkaline phosphatase activity was found to increase significantly (p < 0.01) throughout the ripening stages from 0.0044 ± 0.0006 to 0.0135 ± 0.0006μmol/min/g fresh weight in the Oblong-shaped variety: ‘Agric pawpaw’ and from 0.0074 ± 0.0012 to 0.033 ± 0.0023μmol/min/g fresh weight in the Pear-shaped variety: ‘Local pawpaw’. These results suggest that acid phosphatase is the main non-specific phosphatase that is responsible for the production and supply of inorganic phosphate in the unripe stage of Carica papaya fruits, while alkaline phosphatase is responsible for the production and supply of inorganic phosphate in the overripe stage. Acid phosphatase and alkaline phosphatase could be obtained from the unripe and overripe stages of Carica papaya fruits respectively for research purposes, for commercial exploitation and industrial gains.Keywords: Ripening; Phosphatases; Carica papaya; Agric pawpaw; Local pawpa

Geological and Geotechnical Evaluation of Gully Erosion at Nguzu Edda, Afikpo Sub-Basin, Southeastern Nigeria

A detailed geological field mapping of Nguzu Edda has revealed that gully erosion and landslides have remained active over the years, posing serious threats to human life, agricultural land, infrastructure and socio-economic activities within the area. Consequently, twelve true representative soil samples from different locations within gully sites have been analyzed based on ASTM and British Standards, to evaluate geologic conditions and geotechnical parameters that influence the gullies problems. The results of the laboratory tests revealed that the soil at the gully sites are predominantly sands (57~99.5 % with mean of 69.7%) with low amount of fines (silt; 0.3~ 22% with average value of 14.6% and clay: 0.2 ~ 21% with average value of 13.9%). The liquid limit (LL) ranged from 15 ~ 31.50 % with mean of 23.32% and the plasticity index (PI) ranged from 2.92~14.42% with mean of 8.95%. The moisture content, permeability, bulk density, porosity, organic matter and pH have average values of 25.31%, 6.04×10-4 cm/sec, 1.55 kg/m3, 40.40, 1.26% and 5.12, respectively. The cation exchange capacity (CEC) is low, which could be attributed to low organic matter, pH and non active clay minerals. With regard to the aforementioned characteristics, the soils at gully sites are loose sands with low amount of fines fraction, hence cohesionless, and are easily exposed to agents/factors of gully erosion. Thus, provides insights about the vulnerable causes of gully erosion and landslides problems that are prevalent in the area. This research addresses, to a great extent the effects of local geology, geotechnical properties of the underlying soil and associated human activities on the formation of gully erosion, in-turn results to landslides within in the study area. Engineering-geologic aspects of soil erosion control were also recommended. Keywords: Afikpo Sub-basin, Geologic conditions, Geotechnical analysis, Gully erosion and Erosion control measures

Effects of Aqueous Extract of Moringa oleifera on Phenylhydrazine-induced Liver Toxicity in Wistar Rats

Moringa oleifera has been shown to have a variety of medicinal benefits, including hepatoprotective effects. Phenylhydrazine has been used to cause hepatotoxicity in experimental models. In this study, an aqueous extract of Moringa oleifera was used to evaluate the ameliorating properties of phenylhydrazine-induced liver damage by assessing liver enzymes and histoarchitecture employing twenty-five (25) mature Wistar rats were divided into five (5) groups. Group A received 1 ml distilled water, Group B received 50 mg/kg phenylhydrazine twice in 48 hours, Group C received 500 mg/kg body weight of Moringa oleifera aqueous extract for four (4) weeks, Group D received 50 mg/kg phenylhydrazine twice in 48 hours and 500 mg/kg body weight of Moringa oleifera aqueous extract for four (4) weeks, and Group E received 50 mg/kg of phenylhydrazine twice in forty-eight (48) hours and 70 mg/kg body weight of Silymarin for four (4) weeks.  After the administration of phenylhydrazine, significant increases (P<0.05) in the mean concentrations of liver enzymes (ALP, AST, ALT) and total protein were observed, whereas treatment with Moringa oleifera resulted in a reversal of those parameters to values comparable to the control and the standard drug - Silymarin. Overall, the results showed that Moringa oleifera aqueous extract had considerable hepatoprotective capability against phenylhydrazine-induced hepatotoxicity

Possible association between ABCC8 C49620T polymorphism and type 2 diabetes in a Nigerian population

The association between ABCC8 gene C49620T polymorphism and type 2 diabetes (T2D) in populations of diverse ethnic backgrounds has been reported. However, such occurrence in an African population is yet to be established. This case-control study involving 73 T2D and 75 non-diabetic (ND) patients investigated the occurrence of this polymorphism among T2D patients in Nigeria and assessed its relationship with body lipids of patients. Demographic and clinical characteristics of patients were collected and lipid profile indices including total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL) and high density lipoprotein (HDL) were assayed. Restriction fragment length polymorphism-PCR (RFLP-PCR) was employed to genotype the ABCC8-C49620T polymorphism using PstI restriction enzyme. This study revealed significantly (p 0.05) of T2D for the unadjusted codominant, dominant and recessive models. Following age adjustment, the mutant genotypes (CT and TT) showed significant (p<0.05) risk of T2D for all the models with the recessive model presenting the greatest risk of T2D (OR: 2.39, 95% CI: 1.16-4.91, p<0.018). The TT genotype significantly (p<0.05) associated with high level of HDL and reduced levels of TC, TG and LDL in non-diabetic patients but was not associated with any of the demographic and clinical characteristics among T2D patients. ABCC8 C49620T polymorphism showed possible association with T2D marked by predominance of the mutant TT genotype in T2D patients. However, the relationship between TT genotype and lipid abnormalities for possible beneficial effect on people suffering from T2D is unclear

Cucumeropsis mannii seed oil ameliorates Bisphenol‐A‐induced adipokines dysfunctions and dyslipidemia

From Wiley via Jisc Publications RouterHistory: received 2022-12-25, rev-recd 2023-01-07, accepted 2023-02-06, pub-electronic 2023-02-18Article version: VoRPublication status: PublishedThis study demonstrated the therapeutic potentials of Cucumeropsis mannii seed oil (CMSO) capable of alleviating BPA‐induced dyslipidemia and adipokine dysfunction. In this study, we evaluated the effects of CMSO on adipokine dysfunctions and dyslipidemia in bisphenol‐A (BPA)‐induced male Wistar rats. Six‐week‐old 36 albino rats of 100–200 g weight were assigned randomly to six groups, which received varied doses of BPA and/or CMSO. The administration of BPA and CMSO was done at the same time for 42 days by oral intubation. The adipokine levels and lipid profile were measured in adipose tissue and plasma using standard methods. BPA induced significant (p < .05) increases in triglycerides, cholesterol, leptin, LDL‐C, and atherogenic and coronary risk indices in adipose tissue and plasma, as well as a decrease in adiponectin and HDL‐C levels in Group II animals. BPA administration significantly (p < .05) elevated Leptin levels and reduced adiponectin levels. BPA plus CMSO reduced triglycerides, cholesterol, leptin, LDL‐C, and atherogenic and coronary risk indices while increasing adiponectin levels and HDL‐C in adipose tissue and plasma (p < .05). The results showed that BPA exposure increased adipose tissue as well as serum levels of the atherogenic index, triglycerides, cholesterol, coronary risk index, LDL‐C, leptin, and body weight with decreased adiponectin levels and HDL‐C. Treatment with CMSO reduced the toxicities caused by BPA in rats by modulating the body weight, adiponectin/leptin levels, and lipid profiles in serum and adipose tissue. This study has shown that CMSO ameliorates BPA‐induced dyslipidemia and adipokine dysfunctions. We suggest for further clinical trial to establish the clinical applications

Monitoring of microbial hydrocarbon remediation in the soil

Bioremediation of hydrocarbon pollutants is advantageous owing to the cost-effectiveness of the technology and the ubiquity of hydrocarbon-degrading microorganisms in the soil. Soil microbial diversity is affected by hydrocarbon perturbation, thus selective enrichment of hydrocarbon utilizers occurs. Hydrocarbons interact with the soil matrix and soil microorganisms determining the fate of the contaminants relative to their chemical nature and microbial degradative capabilities, respectively. Provided the polluted soil has requisite values for environmental factors that influence microbial activities and there are no inhibitors of microbial metabolism, there is a good chance that there will be a viable and active population of hydrocarbon-utilizing microorganisms in the soil. Microbial methods for monitoring bioremediation of hydrocarbons include chemical, biochemical and microbiological molecular indices that measure rates of microbial activities to show that in the end the target goal of pollutant reduction to a safe and permissible level has been achieved. Enumeration and characterization of hydrocarbon degraders, use of micro titer plate-based most probable number technique, community level physiological profiling, phospholipid fatty acid analysis, 16S rRNA- and other nucleic acid-based molecular fingerprinting techniques, metagenomics, microarray analysis, respirometry and gas chromatography are some of the methods employed in bio-monitoring of hydrocarbon remediation as presented in this review

Globally, songs and instrumental melodies are slower and higher and use more stable pitches than speech: A Registered Report

Both music and language are found in all known human societies, yet no studies have compared similarities and differences between song, speech, and instrumental music on a global scale. In this Registered Report, we analyzed two global datasets: (i) 300 annotated audio recordings representing matched sets of traditional songs, recited lyrics, conversational speech, and instrumental melodies from our 75 coauthors speaking 55 languages; and (ii) 418 previously published adult-directed song and speech recordings from 209 individuals speaking 16 languages. Of our six preregistered predictions, five were strongly supported: Relative to speech, songs use (i) higher pitch, (ii) slower temporal rate, and (iii) more stable pitches, while both songs and speech used similar (iv) pitch interval size and (v) timbral brightness. Exploratory analyses suggest that features vary along a “musi-linguistic” continuum when including instrumental melodies and recited lyrics. Our study provides strong empirical evidence of cross-cultural regularities in music and speech

The potential of cement stabilized shale quarry dust for possible use as road foundation material

Abstract This research investigated the potential of cement stabilized shale quarry dust for possible use as road foundation material. From the results, the shale consists mainly of SiO2 (27.7–60.1%), Al2O3 (7.30–17.9%), CaO (3.91–32.3%) and LOI (4.93–18.8%). The shale quarry dust (SQD) in its natural state had an average liquid limit (LL) as 38%, plasticity index (PI) as 24% and fines fraction as 14%, which is regarded as poor material according to American Society for Testing and Materials recommendation. The plasticity, compaction, California bearing ratio (CBR) and unconfined compressive strength (UCS) of the SQD were evaluated in its natural state and when mixed with varying percentages of cement. When stabilized with 3% cement, the strength parameters such as CBR and UCS showed improvement by about 20 and 25%, respectively, while the swellability indicators; plasticity and shrinkage limit reduced by about 56.2 and 46.6%, respectively, suggesting substantial improvement in the volume stability, strength and stress–strain properties and continued strength gain with time of the cement treated materials. The presence of the carbonate minerals contributed to the improved strength of the SQD for a given cement type and dosage. The beneficial changes in geotechnical properties of SQD when treated with 6% Portland cement, indicate that treated shale material satisfy most Nigerian specifications for highway fill and embankment, base and sub-base material. Their use, in heavy traffic highway, however, may be handled with caution as the continued high LL may likely imply some swelling on moisture influx