Morphology of Camellia Sinensis L. leaves as marker of white tea authenticity

Received: February 1st, 2021 ; Accepted: April 24th, 2021 ; Published: August 18th, 2021 ; Correspondence: [email protected] is one of the most common drinks in the world. Classic tea is obtained by brewing the leaves of the Camellia sinensis L plant in hot water. However, even the leaves collected from the same branch of the same tea bush can have completely different anatomical, biochemical and taste characteristics. White tea is the youngest, immature apical leaves of the tea bush (fleshes) together with leaf buds (tips) which are is considered the most valuable parts of teaplant. The chemical composition of tea is studied in sufficient detail, however, there are still no uniform criteria for determining the authenticity of white tea leaves, which creates great preconditions for falsifying this most valuable type of raw material. The aim of this study was to study the macroand microstructure of white tea leaves from different manufacturers and to determine the morphological markers of the authenticity of white tea leaves. The objects of research were white tea from the Nandana Tea Factory (Sri Lanka) and white tea from an unknown manufacturer, purchased from a local tea shop. The study of raw materials was carried out in accordance with the requirements of GF XIV OFS 1.5.1.0003.15 ‘Leaves’ and OFS 1.5.3.0003.15 ‘Technique of microscopic and microchemical examination of medicinal plants and herbal medicinal products.’ The work was carried out on the basis of the laboratories of the Department of Food Technologies of FGBOU VO Saratov GAU named after N.I. Vavilov, and the Department of General Biology, Pharmacognosy and Botany, Saratov State Medical University named after V.I. Razumovsky Ministry of Health of Russia. Studies of the structure of white tea leaves from various manufacturers have shown that the structure and presence of morphological elements of leaves, such as hairs, stomata, leaf edge, druses, sclereids, differ markedly and can serve as reliable markers for identifying the variety of tea

Transbronchial Guidance for Balloon Pulmonary Angioplasty in CTEPH: The Safe Guidewire Passage Through the Occlusion

[No abstract available

The role of non-commercial cyprinids in maintenance and spread of the opisthorchiasis focus in the middle Ob River basin (Tomsk region, Russia)

The study assessed the role of non-commercial cyprinid species in maintaining the opisthorchiasis focus in the middle Ob River basin, Tomsk region, Russia. The source of O. felineus infection for humans and carnivores is fish of the family Cyprinidae. This is the most numerous family, 14 species live in the middle Ob River basin, which includes 6 commercial species and 8 non-commercial species. This study aimed to investigate the current situation on infestation of non-commercial cyprinids with O. felineus metacercariae and their role in maintaining and spreading the natural focus of opisthorchiasis in the middle Ob River basin. We investigated 4 non-commercial species (tench, sunbleak, common bleak, gudgeon), which are highly abundant in water bodies. Tench, common bleak and gudgeon are objects of amateur fishing. These species are traditionally included in the diet of the local population. Opisthorchis felineus metacercariae were recorded in muscles of all the examined fish species. The identification of metacercariae was confirmed by morphological methods and PCR diagnostics. Tench and sunbleak are the main sources of opisthorchiasis infection in the floodplain lakes of the Ob River basin (the prevalence of tench infection is 89.3% and mean intensity of infection is 11.2 metacercariae per fish, the prevalence of sunbleak infection is 50.9% and the intensity of infection is 4.25 metacercariae per fish). The prevalence of infection in the introduced common bleak from the rivers of the middle Ob River basin is rapidly increasing from 2.4 (2016-2018) to 37.5% (2020-2021), and mean intensity of infection increased from 1 to 4.15. The epizootic state of water bodies in the middle Ob River basin remains unfavorable in relation to opisthorchiasis. Tench, common bleak and sunbleak, along with ide and dace, are the main source of infection for humans and animals, which is evidenced by high infection with Opisthorchis felineus metacercariae in these numerous fish species. They pose the greatest danger of infection of people and animals with opisthorchiasis. These species should be included in the campaign to avoid raw and poorly cooked fish in the diet. In addition, such species as roach, bream and sunbleak also pose the danger of infection with opisthorchiasis, but to a lesser extent

Principles of healthcare management for patients with pulmonary hypertension: focus on continuity

In managing care for patients with rare, rapidly progressive pathologies such as pulmonary hypertension (PH) and chronic thromboembolic hypertension (CTEPH), early diagnosis and speed of routing are of particular importance. Reducing the time spent on patient routing reduces the risk of disease progression, the rate of hospitalizations, and thereby the socioeconomic burden of the disease.In the Republic of Karelia, an algorithm for routing patients with PH and CTEPH has been created, which is an example of a successful healthcare management

The World Largest Focus of the Opisthorchiasis in the Ob-Irtysh Basin, Russia, Caused by <em>Opisthorchis felineus</em>

The world’s largest focus of opisthorchiasis caused by cat fluke Opisthorchis felineus Rivolta, 1884, is associated with the Ob-Irtysh basin (Russia). The chapter provides data on the history of discovery and the study of opisthorchiasis. Features of the morphology and life cycle of O. felineus are described. Data on the infection of intermediate hosts (mollusks and cyprinids fish) are provided. Species of fish that have important epizootological significance are indicated. The incidence of opisthorchiasis in the people of different age and social groups, clinical manifestations, pathogenesis, and complications is discussed. The climatic and social factors that contribute to maintaining the focus of opisthorchiasis are described. The measures of personal and social prevention of the people are given

Experimental Stand for Investigations of Insulator Degradation and Electrode Erosion in High-current Breaker

An experimental stand for studies of electric arc, electrode erosion and insulator degradation processes in high-current circuit breakers and some preliminary experimental data is described. The setup includes a discharge chamber, a capacitive energy storage with capacitance of 0.11 F, voltage up to 10 kV, and all necessary diagnostic techniques. The stand is designed for modeling current pulse with amplitude of 3–150 kA and duration of the first half period of 1.0–3.0  ms during the process of disconnecting the ring and the pin contacts. The arc is cooled by transverse gas blowing at pressure in the chamber of 0.5–3 MPa. Acquired experimental data can be used for verification of the modelling results of the heat transfer processes in the discharge chamber. At the stand, advanced composite materials based on carbon and iron-copper pseudoalloy are studied

Analysis of Multiple Infection in Ixodic Ticks <i>Dermacentor reticulatus</i> in a Combined Natural Focus of Vector-Borne Infections in the Tomsk Region

The aim of the study was to characterize mono- and mixed infection with causative agents of vector-borne infections in Dermacentor reticulatus ticks inhabiting one of the anthropourgic foci of the Tomsk Region. Materials and methods. D. reticulatus ticks were collected from vegetation “on the flag” in the forest park zones of the southern part of Tomsk in August and September, 2020–2021. To isolate nucleic acids from homogenates of mites and subsequent PCR diagnostics, kits of the RealBest series (“Vector-Best”, Novosibirsk) were used. 184 individual samples of nucleic acids were examined for the presence of molecular-genetic markers of tick-borne encephalitis virus (TBEV), pathogenic borrelia, babesia, rickettsia, anaplasma, and ehrlichia. Results and discussion. The spectrum of pathogens detected through PCR assay of ticks collected in one of the anthropurgic foci of the Tomsk Region in 2020 and 2021 includes TBEV, pathogenic borrelia, rickettsia, and babesia. The highest rate of D. reticulatus infection has been established in relation to candidate pathogens of tick-borne rickettsioses (Rickettsia sp.) and babesioses (Babesia sp.). The overall frequency of co-infection in D. reticulatus was 12.88 %. Seven different associations of pathogens infecting D. reticulatus have been identified. The majority (88.24 %) of the detected cases of mixed infection were rickettsia associations. Double infection has been registered in 94 % of the total number of mixed variants. Markers of four tick-borne infection pathogens simultaneously (Rickettsia species DNA, Babesia species DNA, Borrelia burgdorferi s.l. DNA and TBEV RNA) have been found in one sample. Changes in areal of the D. reticulatus against the background of an increase in their numbers, determines the need to analyze the epidemiological significance of the ticks of this species in combined foci of tick-borne infections, especially in the zone of sympatry with ixodic ticks of other species

2020 Clinical practice guidelines for Pulmonary hypertension, including chronic thromboembolic pulmonary hypertension

Russian Society of Cardiology (RSC)With the participation: Association of Cardiovascular Surgeons of Russia, Russian Respiratory Society, Federation of Anesthesiologists and Resuscitators, Association of Rheumatologists of Russia, National Congress of Radiation Diagnosticians