Trypanosome mRNAs share a common 5' spliced leader sequence.

A 5'-terminal leader sequence of 35 nucleotides was found to be present on multiple trypanosome RNAs. Based on its representation in cDNA libraries, we estimate that many, if not all, trypanosome mRNAs contain this leader. This same leader was originally identified on mRNAs encoding the molecules responsible for antigenic variation, variant surface glycoproteins. Studies of selected cDNAs containing this leader sequence revealed that leader-containing transcripts can be stage-specific, stage-regulated, or constitutive. They can be abundant or rare, and transcribed from single or multigene families. No linkage between the genomic leader sequences and the structural gene exons was observed. Possible mechanisms by which the leader sequences are added to trypanosome mRNAs are discussed

Identification of a small RNA containing the trypanosome spliced leader: a donor of shared 5' sequences of trypanosomatid mRNAs?

The 35 nucleotide spliced leader (SL) sequence is found on the 5' end of numerous trypanosome mRNAs, yet the tandemly organized reiteration units encoding this leader are not detectably linked to any of these structural genes. Here we report the presence of a class of discrete small SL RNA molecules that are derived from the genomic SL reiteration units of Trypanosoma brucei, Trypanosoma cruzi, and Leptomonas collosoma. These small SL RNAs are 135, 105, and 95 nucleotides, respectively, and contain a 5'-terminal SL or SL-like sequence. S1 nuclease analyses demonstrate that these small SL RNAs are transcribed from continuous sequence within the respective SL reiteration units. With the exception of the SL sequence and a concensus donor splice site immediately following it, these small RNAs are not well conserved. We suggest that the small SL RNAs may function as a donor of the SL sequence in an intermolecular process that places the SL at the 5' terminus of many trypanosomatid mRNAs

Non-Hermitian Delocalization and Eigenfunctions

Recent literature on delocalization in non-Hermitian systems has stressed criteria based on sensitivity of eigenvalues to boundary conditions and the existence of a non-zero current. We emphasize here that delocalization also shows up clearly in eigenfunctions, provided one studies the product of left- and right-eigenfunctions, as required on physical grounds, and not simply the squared modulii of the eigenfunctions themselves. We also discuss the right- and left-eigenfunctions of the ground state in the delocalized regime and suggest that the behavior of these functions, when considered separately, may be viewed as ``intermediate'' between localized and delocalized.Comment: 8 pages, 11 figures include

Drivers of Microbial Risk for Direct Potable Reuse and de Facto Reuse Treatment Schemes: The Impacts of Source Water Quality and Blending.

Although reclaimed water for potable applications has many potential benefits, it poses concerns for chemical and microbial risks to consumers. We present a quantitative microbial risk assessment (QMRA) Monte Carlo framework to compare a de facto water reuse scenario (treated wastewater-impacted surface water) with four hypothetical Direct Potable Reuse (DPR) scenarios for Norovirus, Cryptosporidium, and Salmonella. Consumer microbial risks of surface source water quality (impacted by 0-100% treated wastewater effluent) were assessed. Additionally, we assessed risks for different blending ratios (0-100% surface water blended into advanced-treated DPR water) when source surface water consisted of 50% wastewater effluent. De facto reuse risks exceeded the yearly 10-4 infections risk benchmark while all modeled DPR risks were significantly lower. Contamination with 1% or more wastewater effluent in the source water, and blending 1% or more wastewater-impacted surface water into the advanced-treated DPR water drove the risk closer to the 10-4 benchmark. We demonstrate that de facto reuse by itself, or as an input into DPR, drives microbial risks more so than the advanced-treated DPR water. When applied using location-specific inputs, this framework can contribute to project design and public awareness campaigns to build legitimacy for DPR

A qualitative analysis of the interfaces between urban underground metro infrastructure and its environment in London

As urban environments densify and cities across the world employ urban underground metros for the effective rapid movement of millions of people a day, there is an essential need to ensure the safe continued presence and operation of those metros. To achieve this, the authors argue there is a need for a more detailed qualitative analysis of how urban underground metro infrastructure and its environment interface. Failure to do so could potentially lead to the development of inaccurate asset management data. This would subsequently lead to the implementation of flawed Building Information Modelling processes for 2, 3, and 4D modelling and mapping, of existing and proposed infrastructure. Following a review of existing asset management approaches which confirms the need for qualitative approaches to the analyses of the interfaces, this paper presents findings from a detailed case study, in the Bayswater area of the City of Westminster in West London, UK.The processes employed for the detailed case study form part of proposed (ultimately) standardised approaches to the gathering, analysis, and sharing, of multi-disciplinary evidence-based data, developed by the authors. Such data is essential to enable effective asset and urban management processes, now and for the future

Symbiotic stars in X-rays III: Suzaku observations

We describe the X-ray emission as observed with Suzaku from five symbiotic stars that we selected for deep Suzaku observations after their initial detection with ROSAT, ASCA and Swift. We find that the X-ray spectra of all five sources can be adequately fit with absorbed, optically thin thermal plasma models, with either single- or multi-temperature plasmas. These models are compatible with the X-ray emission originating in the boundary layer between an accretion disk and a white dwarf. The high plasma temperatures of kT$~>3$ keV for all five targets were greater than expected for colliding winds. Based on these high temperatures, as well as previous measurements of UV variability and UV luminosity, and the large amplitude of X-ray flickering in 4 Dra, we conclude that all five sources are accretion-powered through predominantly optically thick boundary layers. Our X-ray data allow us to observe a small, optically thin portion of the emission from these boundary layers. Given the time between previous observations and these observations, we find that the intrinsic X-ray flux and the intervening absorbing column can vary by factors of three or more on a time scale of years. However, the location of the absorber and the relationship between changes in accretion rate and absorption are still elusive.Comment: 14 pages, 3 figures and 3 tables. Accepted to published 04/15/2016. arXiv admin note: substantial text overlap with arXiv:1505.0063

Topological defects, pattern evolution, and hysteresis in thin magnetic films

Nature of the magnetic hysteresis for thin films is studied by the Monte-Carlo simulations. It is shown that a reconstruction of the magnetization pattern with external field occurs via the creation of vortex-antivortex pairs of a special kind at the boundaries of stripe domains. It is demonstrated that the symmetry of order parameter is of primary importance for this problem, in particular, the in-plane magnetic anisotropy is necessary for the hysteresis.Comment: Accepted to EPL; 7 pages, 3 color figure