93 research outputs found
The Atmospheric Monitoring Strategy for the Cherenkov Telescope Array
The Imaging Atmospheric Cherenkov Technique (IACT) is unusual in astronomy as
the atmosphere actually forms an intrinsic part of the detector system, with
telescopes indirectly detecting very high energy particles by the generation
and transport of Cherenkov photons deep within the atmosphere. This means that
accurate measurement, characterisation and monitoring of the atmosphere is at
the very heart of successfully operating an IACT system. The Cherenkov
Telescope Array (CTA) will be the next generation IACT observatory with an
ambitious aim to improve the sensitivity of an order of magnitude over current
facilities, along with corresponding improvements in angular and energy
resolution and extended energy coverage, through an array of Large (23m),
Medium (12m) and Small (4m) sized telescopes spread over an area of order
~km. Whole sky coverage will be achieved by operating at two sites: one in
the northern hemisphere and one in the southern hemisphere. This proceedings
will cover the characterisation of the candidate sites and the atmospheric
calibration strategy. CTA will utilise a suite of instrumentation and analysis
techniques for atmospheric modelling and monitoring regarding pointing
forecasts, intelligent pointing selection for the observatory operations and
for offline data correction.Comment: 6 pages. To appear in the proceedings of the Adapting to the
Atmosphere conference 201
Diffusion and transport in the human interphase cell nucleus - FCS experiments compared to simulations.
Despite the succesful linear sequencing of the human genome the three-dimensional arrangement of chromatin,
functional, and structural components is still largely unknown. Molecular transport and diffusion are important
for processes like gene regulation, replication, or repair and are vitally influenced by the structure. With a
comparison between fluorescence correlation spectroscopy (FCS) experiments and simulations we show here an
interdisciplinary approach for the understanding of transport and diffusion properties in the human interphase
cell nucleus.
For a long time the interphase nucleus has been viewed as a 'spaghetti soup' of DNA without much internal
structure, except during cell division. Only recently has it become apparent that chromosomes occupy distinct
'territories' also in interphase. Two models for the detailed folding of the 30 nm chromatin fibre within these
territories are under debate: In the Random-Walk/Giant-Loop-model big loops of 3 to 5 Mbp are attached to a
non-DNA backbone. In the Multi-Loop-Subcompartment (MLS) model loops of around 120 kbp are forming
rosettes which are also interconnected by the chromatin fibre. Here we show with a comparison between
simulations and experiments an interdisciplinary approach leading to a determination of the three-dimensional
organization of the human genome: For the predictions of experiments various models of human interphase
chromosomes and the whole cell nucleus were simulated with Monte Carlo and Brownian Dynamics methods.
Only the MLS-model leads to the formation of non-overlapping chromosome territories and distinct functional
and dynamic subcompartments in agreement with experiments. Fluorescence in situ hybridization is used for the
specific marking of chromosome arms and pairs of small chromosomal DNA regions. The labelling is visualized
with confocal laser scanning microscopy followed by image reconstruction procedures. Chromosome arms show
only small overlap and globular substructures as predicted by the MLS-model. The spatial distances between
pairs of genomic markers as function of their genomic separation result in a MLS-model with loop and linker
sizes around 126 kbp. With the development of GFP-fusion-proteins it is possible to study the chromatin
distribution and dynamics resulting from cell cycle, treatment by chemicals or radiation in vivo. The chromatin
distributions are similar to those found in the simulation of whole cell nuclei of the MLS-model. Fractal analysis
is especially suited to quantify the unordered and non-euclidean chromatin distribution of the nucleus. The
dynamic behaviour of the chromatin structure and the diffusion of particles in the nucleus are also closely
connected to the fractal dimension. Fractal analysis of the simulations reveal the multi-fractality of
chromosomes. First fractal analysis of chromatin distributions in vivo result in significant differences for
different morphologies and might favour a MLS-model-like chromatin distribution. Simulations of fragment
distributions based on double strand breakage after carbon-ion irradiation differ in different models. Here again a
comparison with experiments favours a MLS-model.
FCS in combination with a scanning device is a suitable tool to study the diffusion characteristics of fluorescent
proteins in living cell nuclei with high spatial resolution. Computer simulations of the three-dimensional
organization of the human interphase nucleus allows a detailed test of theoretical models in comparison to
experiments. Diffusion and transport in the nucleus are most appropriately described with the concept of
obstructed diffusion. A large volume fraction of the nucleus seems to contain a cytosol-like liquid with an
apparent viscosity 5 times higher than in water. The geometry of particles and structure as well as their
interactions influence the mobilities in terms of speed and spatial coverage. A considerable amount of genomic
sites is accessible for not too large particles. FCS experiments and simulations based on the polymer model are
in a good agreement. Using recently developed in vivo chromatin markers, a detailed study of mobility vs.
structure is subject of current work
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Recommendations for processing atmospheric attenuated backscatter profiles from Vaisala CL31 ceilometers
Ceilometer lidars are used for cloud base height detection, to probe aerosol layers in the atmosphere (e.g. detection of elevated layers of Saharan dust or volcanic ash), and to examine boundary layer dynamics. Sensor optics and acquisition algorithms can strongly influence the observed attenuated backscatter profiles; therefore, physical interpretation of the profiles requires careful application of corrections. This study addresses the widely deployed Vaisala CL31 ceilometer. Attenuated backscatter profiles are studied to evaluate the impact of both the hardware generation and firmware version. In response to this work and discussion within the CL31/TOPROF user community (TOPROF, European COST Action aiming to harmonise ground-based remote sensing networks across Europe), Vaisala released new firmware (versions 1.72 and 2.03) for the CL31 sensors. These firmware versions are tested against previous versions, showing that several artificial features introduced by the data processing have been removed. Hence, it is recommended to use this recent firmware for analysing attenuated backscatter profiles. To allow for consistent processing of historic data, correction procedures have been developed that account for artefacts detected in data collected with older firmware. Furthermore, a procedure is proposed to determine and account for the instrument-related background signal from electronic and optical components. This is necessary for using attenuated backscatter observations from any CL31 ceilometer. Recommendations are made for the processing of attenuated backscatter observed with Vaisala CL31 sensors, including the estimation of noise which is not provided in the standard CL31 output. After taking these aspects into account, attenuated backscatter profiles from Vaisala CL31 ceilometers are considered capable of providing valuable information for a range of applications including atmospheric boundary layer studies, detection of elevated aerosol layers, and model verification
The Dynamic Exponent of the Two-Dimensional Ising Model and Monte Carlo Computation of the Sub-Dominant Eigenvalue of the Stochastic Matrix
We introduce a novel variance-reducing Monte Carlo algorithm for accurate
determination of autocorrelation times. We apply this method to two-dimensional
Ising systems with sizes up to , using single-spin flip dynamics,
random site selection and transition probabilities according to the heat-bath
method. From a finite-size scaling analysis of these autocorrelation times, the
dynamical critical exponent is determined as (12)
Nonequilibrium relaxation of the two-dimensional Ising model: Series-expansion and Monte Carlo studies
We study the critical relaxation of the two-dimensional Ising model from a
fully ordered configuration by series expansion in time t and by Monte Carlo
simulation. Both the magnetization (m) and energy series are obtained up to
12-th order. An accurate estimate from series analysis for the dynamical
critical exponent z is difficult but compatible with 2.2. We also use Monte
Carlo simulation to determine an effective exponent, z_eff(t) = - {1/8} d ln t
/d ln m, directly from a ratio of three-spin correlation to m. Extrapolation to
t = infinity leads to an estimate z = 2.169 +/- 0.003.Comment: 9 pages including 2 figure
Finite Size Scaling and Critical Exponents in Critical Relaxation
We simulate the critical relaxation process of the two-dimensional Ising
model with the initial state both completely disordered or completely ordered.
Results of a new method to measure both the dynamic and static critical
exponents are reported, based on the finite size scaling for the dynamics at
the early time. From the time-dependent Binder cumulant, the dynamical exponent
is extracted independently, while the static exponents and
are obtained from the time evolution of the magnetization and its higher
moments.Comment: 24 pages, LaTeX, 10 figure
Measurement and simulation of the 16/17 April 2010 Eyjafjallajökull volcanic ash layer dispersion in the northern Alpine region
The spatial structure and the progression speed of the first ash layer from the Icelandic Eyjafjallajökull volcano which reached Germany on 16/17 April is investigated from remote sensing data and numerical simulations. The ceilometer network of the German Meteorological Service was able to follow the progression of the ash layer over the whole of Germany. This first ash layer turned out to be a rather shallow layer of only several hundreds of metres thickness which was oriented slantwise in the middle troposphere and which was brought downward by large-scale sinking motion over Southern Germany and the Alps. Special Raman lidar measurements, trajectory analyses and in-situ observations from mountain observatories helped to confirm the volcanic origin of the detected aerosol layer. Ultralight aircraft measurements permitted the detection of the arrival of a second major flush of volcanic material in Southern Germany. Numerical simulations with the Eulerian meso-scale model MCCM were able to reproduce the temporal and spatial structure of the ash layer. Comparisons of the model results with the ceilometer network data on 17 April and with the ultralight aircraft data on 19 April were satisfying. This is the first example of a model validation study from this ceilometer network data
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